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Fluorescent probes for the detection of reactive oxygen species in human spermatozoa

Asian Journal of Andrology 2020年第5期22卷 465-471页

作者： Sara Escada-Rebelo Francisca G Mora Ana P Sousa teresa almeida-santos Artur Paiva Joao Ramalho-santos phD Programme in Experimental Biology and Biomedicine CNC-Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbra 3004-504Portugal Biology of Reproduction and Stem Cell Group CNC-Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbra 3004-504Portugal IIIUC-Institute for Interdisciplinary Research Casa Costa AlemaoUniversity of CoimbraCoimbra 3030-789Portugal Reproductive Medicine Unit University Hospitals of CoimbraCoimbra 3004-561Portugal Faculty of Medicine University of CoimbraCoimbra 3000-370Portugal Clinical Pathology Unit University Hospitals of CoimbraCoimbra 3004-561Portugal Department of Life Sciences University of CoimbraCoimbra 3000-456Portugal

Reactive oxygen species(ROS)production is a by-product of mitochondrial activity and is necessary for the acquisition of the capacitated state,a requirement for functional ***,an increase in oxidative stress,due to an abnormal production of ROS,has been shown to be related to loss of sperm function,highlighting the importance of an accurate detection of sperm ROS,given the specific nature of this *** this work,we tested a variety of commercially available fluorescent probes to detect ROS and reactive nitrogen species(RNS)in human sperm,to define their *** both flow cytometry(FC)and fluorescence microscopy(FM),we confirmed that MitoSOX™Red and dihydroethidium(DHE)detect superoxide anion(as determined using antimycin A as a positive control),while DAF-2A detects reactive nitrogen species(namely,nitric oxide).For the first time,we also report that RedoxSensor™Red CC-1,CellROX®Orange Reagent,and MitoPYl seem to be mostly sensitive to hydrogen peroxide,but not ***,mean fluorescence intensity(and not percentage of labeled cells)is the main parameter that can be reproducibly monitored using this type of methodology.

关键词： flow cytometry fluorescent probes human spermatozoa oxidative stress reactive oxygen species

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3D City Models as a Visual Support Tool for the Analysis of Buildings Seismic Vulnerability: The Case of Lisbon

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International Journal of Disaster Risk Science 2017年第3期8卷 308-325页

作者： Paula Redweik Paula Teves-Costa Inês Vilas-Boas teresa santos Instituto Dom Luiz Faculdade de CiênciasUniversidade de Lisboa ESRI Portugal CICS.NOVA–Centro Interdisciplinar de Ciências Sociais Faculdade de Ciências Sociais e HumanasUniversidade Nova de Lisboa

3D city models associate a database of a city to a rigorous geospatial representation that is close to the visible reality by combining appearance, geometry, and semantics. As such, these models may include tools that convey attribute analysis, not only at the statistical level but also in terms of visual appearance of the objects, thus allowing the generation of new knowledge about a phenomenon or its effects. Lisbon is a city with a moderate level of seismic risk, and has been hit in the past by strong earthquakes. Several seismic vulnerability studies of buildings have been carried out in Lisbon. These studies generate different scenarios that consider the magnitude and source location of potential earthquakes. The results of these studies were presented as maps obtained from crossing information contained in 2D layers and presented in a GIS environment. In the present study a seismic vulnerability assessment of Lisbon's buildings held in 2D is extended to the third dimension, which refines the resolution and the set of parameters and explores the gains of spatial analysis in 3D representations. The numerous parameters that contribute to assess seismic vulnerability of buildings may be analyzed individually or simultaneously as well as in multiple seismic scenarios. Although covering all residential buildings in Lisbon, the application of the 3Dcity model is demonstrated with more detail in three pilot areas.

关键词： 3D-GIS 3D models Building vulnerability Lisbon Seismic scenarios

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Mangiferin,a natural xanthone,accelerates gastrointestinal transit in mice involving cholinergic mechanism

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World Journal of Gastroenterology 2012年第25期18卷 3207-3214页

作者： Talita Cavalcante Morais Synara Cavalcante Lopes Karine Maria Martins Bezerra Carvalho Bruno Rodrigues Arruda Francisco Thiago Correia de Souza Maria teresa Salles Trevisan Vietla Satyanarayana Rao Flávia almeida santos Department of Physiology and Pharmacology Brazilian SemiArid Institute of BiomedicineFaculty of MedicineFederal University of CearáFortaleza 60430-270Brazil Post-Graduate Programme in Medical Sciences Faculty of MedicineFederal University of CearáFortaleza 60430-140Brazil Department of Organic and Inorganic Chemistry Federal University of CearáFortaleza 60451-970Brazil

AIM： To investigate the effects of mangiferin on gas- trointestinal transit （GIT） in normal and constipated mice, together with the possible ***： Intragastrically-administered charcoal mealwas used to measure GIT in overnight starved Swiss mice. In the first experiments, mangiferin （3 mg/kg, 10 mg/kg, 30 mg/kg, and 100 mg/kg, po） or tegaserod （1 mg/kg, ip） were administered 30 min before the char- coal meal to study their effects on normal transit. In the second series, mangiferin （30 mg/kg） was tested on delayed GIT induced by several different pharma- cological agonists （morphine, clonidine, capsaicin） or antagonists （ondansetron, verapamil, and atropine） whereas in the third series, mangiferin （30 mg/kg, 100 mg/kg and 300 mg/kg） or tegaserod （1 mg/kg） were tested on 6 h fecal pellets outputted by freely fed mice. The ratio of wet to dry weight was calculated and used as a marker of fecal water content. RESULTS： Mangiferin administered orally significantly （P 〈 0.05） accelerated GIT at 30 mg/kg and 100 mg/kg （89% and 93%, respectively）, similarly to 5-hydroxytrypta- mine4 （5-H%） agonist tegaserod （81%） when compared to vehicle-treated control （63%）. Co-administered man- giferin （30 mg/kg） totally reversed the inhibitory effect of opioid agonist morphine, 5-HT3-receptor antagonist ondansetron and transient receptor potential vanilloid-1 receptor agonist capsaicin on GIT, but only to a partial extent with the GIT-delay induced by ~2-adrenoceptor agonist clonidine, and calcium antagonist verapamil. However, co-administered atropine completely blocked the stimulant effect of mangiferin on GIT, suggesting the involvement of muscarinic acetylcholine receptor activation. Although mangiferin significantly enhanced the 6 h fecal output at higher doses （245.5±10.43 mg vs 161.9±10.82 mg and 227.1±20.11 mg vs 161.9±10.82 mg of vehicle-treated control, at 30 and 100 mg/ kg, P 〈 0.05, respectively）, the effect of tegaserod was more potent （297.4±7.42 mg vs 161.9±1

关键词： Mangiferin Glucosylxanthone Gastrointes-tinal transit Prokinetic action Cholinergic mechanism

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Sustainable living neighbourhoods:Measuring public space quality and walking environment in Lisbon

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Geography and Sustainability 2022年第4期3卷 289-298页

作者： teresa santos Filipa Ramalhete Rui Pedro Julião Nuno Pires Soares Interdisciplinary Centre of Social Sciences(CICS.NOVA) Faculty of Social Sciences and Humanities(NOVA FCSH)Universidade NOVA de LisboaAv.de Berna26 CLisboa 1069-061Portugal Studies Centre on Architecture City and Territory(CEACT)Universidade Autónoma de LisboaRua Fernando Assis Pacheco207Lisboa 1350-300Portugal

Obesity and global warming are two major challengesthat affect communities’health and quality of *** good walking environments,with appropriate pedestrian infrastructure,green areas and access to commercial retail and transport hubs favour liveable,healthy,and sustainable *** Lisbon City Hall has implemented an intervention program in the public space as a strategy to improve environmental quality and mitigate the effects of climate *** program“A square in each neighbourhood”intends not only to reinforce the economic base of Lisbon,namely tourism,restaurants,commerce,and creative activities but also to promote physical activity and improve the quality of living of all *** studying the impact of the public space quality on the walking environment,Campolide,a typical Lisbon neighbourhood,Portugal,which includes one of the requalified squares,was selected.A two-step methodology based on spatial modelling is *** the first step,the public space quality was evaluated based on a set of 13 urban quality ***,intended for the street level,were collected from open data platforms and complemented with a field ***,through spatial analysis,these attributes were used to build the Pedestrian Environment Quality Indicator and the Priority Intervention *** these indicators,walking-friendly streets were mapped and the most critical sites that require priority intervention were identified to prepare for the requalification planning *** street with the highest quality in the study area according to the dimensions evaluated-connectivity,convenience,comfort,cleanliness,and conviviality-has a great offer of trees,bus stops,commercial establishments,commercial diversity,crossings,paper bins/eco points and *** the opposite side of the rank,the street with the lowest quality has no trees or green areas,transport stops,benches and tables,or commercial *** urban quality indicators constitu

关键词： Public space Walking environment Spatial modelling Urban quality indicators Geographic Information Systems

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The road to sustainable use and waste management of plastics in Portugal

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Frontiers of Environmental Science & Engineering 2022年第1期16卷 91-106页

作者： Joana C.Prata Ana LPatrício Silva Armando CDuarte teresa Rocha-santos Centre for Environmental and Marine Studies(CESAM)&Department of Chemistry University of Aveiro3810-193 AveiroPortugal Centre for Environmental and Marine Studies(CESAM)&Department of Biology University of Aveiro3810-193 AveiroPortugal

As a European Union(EU)member,Portugal must comply with reductions in plastic *** Portugal,the 330 items/100 m of beach litter,comprising up to 3.9 million pieces and of which 88%is plastic,is higher than the EU median(149 items/100 m)and must be reduced to 20 items/100 m(94%).Integrative measures are needed to reduce littering and improve plastics’use and disposal under the circular *** this 414 kt of plastic packaging waste,163 kt were declared plastic packaging,140 kt subjected to recycling,and 94 kt to energy *** current recycling rate of plastic packaging(34%)should be improved to reach EU recycling averages(42%)and goals and to provide widespread benefits,considering revenues of 167€/*** a net importer of waste,Portugal could benefit from the valorization of imported *** increased recycling,pyrolysis and gasification could provide short-term alternatives for producing value-added substances from plastic waste,such as hydrogen,consistent with the National Plan of Hydrogen and improving ongoing regulations on single-use *** manuscript provides an integrative view of plastics in Portugal,from use to disposal,providing specific recommendations under the circular economy.

关键词： Single-use plastics Plastic packaging Plastic waste Waste management Waste shipment Lightweight plastic bags

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Plasma glial fibrillary acidic protein and neurofilament light chain for the diagnostic and prognostic evaluation of frontotemporal dementia

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Translational Neurodegeneration 2021年第4期10卷 666-677页

作者： Nuole Zhu Miguel santos-santos Ignacio lllán-Gala Victor Montal teresa Estellés Isabel Barroeta Miren Altuna Javier Arranz Laia Muñoz Olivia Belbin Isabel Sala Maria Belén Sánchez-Saudinós Andrea Subirana Laura Videla Jordi Pegueroles Rafael Blesa Jordi Clarimón Maria Carmona-Iragui Juan Fortea Alberto Lleó Daniel Alcolea Sant Pau Memory Unit Department of NeurologyInstitut d'Investigacions Biomèdiques Sant Pau-Hospital de Sant PauUniversitat Autònoma de Barcelona08041 BarcelonaSpain Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas(CIBERNED) 28031 MadridSpain Autonomous University of Barcelona 08913 BarcelonaSpain Fundación Catalana Sindrome de Down Centre Mèdic Down08029 BarcelonaSpain

Background:Astrocytes play an essential role in neuroinflammation and are involved in the pathogenesis of neurodenegerative *** of glial fibrillary acidic protein(GFAP),an astrocytic damage marker,may help advance our understanding of different neurodegenerative *** this study,we investigated the diagnostic performance of plasma GFAP(pGFAP),plasma neurofilament light chain(pNfL)and their combination for frontotemporal dementia(FTD)and Alzheimer's disease(AD)and their clinical utility in predicting disease ***:pGFAP and pNfL concentrations were measured in 72 FTD,56 AD and 83 cognitively normal(CN)participants using the Single Molecule Array *** the 211 participants,199 underwent cerebrospinal(CSF)analysis and 122 had magnetic resonance *** compared cross-sectional biomarker levels between groups,studied their diagnostic performance and assessed correlation between CSF biomarkers,cognitive performance and cortical *** prognostic performance was investigated,analyzing cognitive decline through group comparisons by ***:Unlike pNfL,which was increased similarly in both clinical groups,pGFAP was increased in FTD but lower than in AD(all P<0.01).Combination of both plasma markers improved the diagnostic performance to discriminate FTD from AD(area under the curve[AUC]:combination 0.78;pGFAP 0.7;pNfL 0.61,all P<0.05).In FTD,pGFAP correlated with cognition,CSF and plasma NfL,and cortical thickness(all P<0.05).The higher tertile of pGFAP was associated with greater change in MMSE score and poor cognitive outcome during follow-up both in FTD(1.40 points annually,hazard ratio[HR]3.82,P<0.005)and in AD(1.20 points annually,HR 2.26,P<0.005).Conclusions:pGFAP and pNfL levels differ in FTD and AD;and their combination is useful for distinguishing between the two *** could also be used to track disease severity and predict greater cognitive decline during follow-up in patients with FTD.

关键词： Glial fibrillary acidic protein Neurofilament Frontotemporal dementia Plasma biomarkers

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Individual factors related to chronic condition in Portuguese adolescents: Highlights from the HBSC/WHO study

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Health 2013年第11期5卷 25-34页

作者： teresa santos Margarida Gaspar de Matos Maria Celeste Simoes Helena Fonseca Maria do Ceu Machado Faculdade de Motricidade Humana(“Social Adventure”Team) Universidade de LisboaLisboaPortugal Centro de Malaria e Doencas Tropicais/IHMT Universidade Nova de LisboaLisboaPortugal Departamento de Pediatria do Hospital de Santa Maria

Background: Adolescence includes a period (10 to 19 years) of profound biopsychosocial changes, constituting potentially difficult challenges, which may become more pronounced in the presence of a chronic condition and its limitations. Responses are not homogeneous and can be quite variable, depending on various specific individual factors. Research comparing adolescents with or without chronic illness, or comparing across different conditions, has been contradictory, not confirming a direct relationship between the degree of suffering and the chronic condition. Objective: To characterize and assess the impact of 1) having a chronic condition (CC) and 2) how CC affects school participation;and its association with life satisfaction and perception of wellness, controlling for demographic factors: age, gender and family socioeconomic status (SES). Methods: 5050 Portuguese adolescents with an average age of 14 years participated in the Health Behaviour in School-aged Children/WHO (HBSC). Results: The majority of the adolescents with CC reported that their conditions did not affect school participation. Adolescents with CC who indicated that CC affected school participation felt more frequently unwell and presented lower life satisfaction. Being a boy, younger and having high family socio economic status (SES) were identified as predictors of higher life satisfaction;on the other hand, being a girl, older, having lower SES, living with CC and feeling that CC affects school participation are predictors for feeling more frequently unwell. Conclusions: These adolescents showed an increased vulnerability, presenting internalized symptoms and lower life satisfaction. Furthermore, when there was simultaneous occurrence of living with CC and that CC affected school participation, the impact was even higher. Thus, it is crucial that future interventions should include the identified predictors, combined with “listen to the voice” of adolescents, throughout the adaptation proces

关键词： Adolescent Health Chronic Condition Health Promotion and Prevention Quality of Life

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Emerging contaminants:A One Health perspective

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The Innovation 2024年第4期5卷 140-170,139页

作者： Fang Wang Leilei Xiang Kelvin Sze-Yin Leung Martin Elsner Ying Zhang Yuming Guo Bo Pan Hongwen Sun Taicheng An Guangguo Ying Bryan WBrooks Deyi Hou Damian EHelbling Jianqiang Sun Hao Qiu Timothy MVogel Wei Zhang Yanzheng Gao Myrna JSimpson Yi Luo Scott XChang Guanyong Su Bryan MWong Tzung-May Fu Dong Zhu Karl JJobst Chengjun Ge Frederic Coulon Jean Damascene Harindintwali Xiankui Zeng Haijun Wang Yuhao Fu Zhong Wei Rainer Lohmann Changer Chen Yang Song Concepcion Sanchez-Cid Yu Wang Ali El-Naggar Yiming Yao Yanran Huang Japhet Cheuk-Fung Law Chenggang Gu Huizhong Shen Yanpeng Gao Chao Qin Hao Li Tong Zhang Natàlia Corcoll Min Liu Daniel SAlessi Hui Li Kristian KBrandt Yolanda Pico Cheng Gu Jianhua Guo Jianqiang Su Philippe Corvini Mao Ye teresa Rocha-santos Huan He Yi Yang Meiping Tong Weina Zhang Fidèle Suanon Ferdi Brahushi Zhenyu Wang Syed AHashsham Marko Virta Qingbin Yuan Gaofei Jiang Louis A.Tremblay Qingwei Bu Jichun Wu Willie Peijnenburg Edward Topp Xinde Cao Xin Jiang Minghui Zheng Taolin Zhang Yongming Luo Lizhong Zhu Xiangdong Li DamiàBarceló Jianmin Chen Baoshan Xing Wulf Amelung Zongwei Cai Ravi Naidu Qirong Shen Janusz Pawliszyn Yong-guan Zhu Andreas Schaeffer Matthias C.Rillig Fengchang Wu Gang Yu James M.Tiedje State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China Department of Chemistry Hong Kong Baptist UniversityHong KongChina HKBU Institute of Research and Continuing Education Shenzhen Virtual University ParkShenzhenChina Technical University of Munich TUM School of Natural SciencesInstitute of Hydrochemistry85748 GarchingGermany School of Resources&Environment Northeast Agricultural UniversityHarbin 150030China Climate Air Quality Research UnitSchool of Public Health and Preventive MedicineMonash UniversityMelbourneVICAustralia Faculty of Environmental Science&Engineering Kunming University of Science&TechnologyKunming 650500China Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300350China Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control School of Environmental Science and EngineeringInstitute of Environmental Health and Pollution ControlGuangdong University of TechnologyGuangzhou 510006China Ministry of Education Key Laboratory of Environmental Theoretical Chemistry South China Normal UniversityGuangzhouGuangdong 510006China Department of Environmental Science Baylor UniversityWacoTXUSA Center for Reservoir and Aquatic Systems Research(CRASR) Baylor UniversityWacoTXUSA School of Environment Tsinghua UniversityBeijing 100084China School of Civil and Environmental Engineering Cornell UniversityIthacaNY 14853USA Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province Zhejiang University of TechnologyHangzhou 310014China School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Laboratoire d’Ecologie Microbienne Universite Claude Bernard Lyon 1UMR CNRS 5557UMR INRAE 1418VetAgro Sup69622 VilleurbanneFrance Department of Pla

Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary *** global efforts to mitigate legacy pollutants,the continuous introduction of new substances remains a major threat to both people and the *** response,global initiatives are focusing on risk assessment and regulation of emerging contaminants,as demonstrated by the ongoing efforts to establish the UN’s Intergovernmental Science-Policy Panel on Chemicals,Waste,and Pollution *** review identifies the sources and impacts of emerging contaminants on planetary health,emphasizing the importance of adopting a One Health *** for monitoring and addressing these pollutants are discussed,underscoring the need for robust and socially equitable environmental policies at both regional and international *** actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.

关键词： pollution planet contaminants

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Effects of Physical Activity Levels on Fatigue Perception in Patients with Parkinson’s Disease and Neurologically Healthy Individuals

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Health 2014年第21期6卷 2927-2933页

作者： Paulo Cezar Rocha dos santos Fabio Augusto Barbieri Diego Orcioli-Silva Lucas Simieli Lilian teresa Bucken Gobbi UNESP Univ Estadual Paulista at Rio Claro Rio Claro/SP Brazil UNESP Univ Estadual Paulista at Bauru Bauru/SP Brazil

Background: Recent researches involving fatigue and Parkinson’s disease (PD) sought to verify its incidence and the impacts of fatigue on quality life of patients with PD. Despite the importance of regular physical activity practice, there are only few studies that verified the influence of the levels of physical activity on fatigue in patients with PD. Objective: The aim of this study was to compare perception of fatigue between individuals with PD and neurologically healthy individuals (control group), considering the physical activity levels;and to verify the relation between physical activity levels and fatigue dimensions for individuals with PD and control group. Methods: Eighty individuals (40 patients with PD and 40 control individuals) participated in this study. Physical activity levels were evaluated through Modified Baecke Questionnaire for Older Adults. Multidimensional Fatigue Inventory (MFI) was used in order to evaluate fatigue dimensions (general, physical and mental fatigue, reduced motivation and activity). MANOVA two-way and Pearson linear correlation test were performed to analyze the data. Results: Patients with PD presented higher levels of fatigue, in all dimensions evaluated by MFI, comparing to control individuals. There was no association between physical activity levels and perception of fatigue for patients with PD and control individuals. Conclusion: Patients with PD showed increased perception of fatigue when compared to control individuals, due to PD characteristics. In addition, the perception of fatigue’s symptom was not influenced and had no relation by the level of physical activity.

关键词： Parkinson’s Disease Symptom of Fatigue Physical Activity Levels Non-Motor Symptom

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Pervasive Business Intelligence: a Marketing Intelligence Framework Proposal

Pervasive Business Intelligence: a Marketing Intelligence Fr...

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International Conference on Business,Economics and Tourism Management（CBETM2012）

作者： teresa Guarda Manuel Filipe santos Filipe Pinto Carlos Silva Joo Loureno Isla - Superior Institute of Languages and Administration of Leiria Universidade do Minho of Guimares Polytechnic Institute of Leiria Isla - Superior Institute of Languages and Administration of Leiria and CIEO - Centre for Spatial and Organizational Dynamics

Nowadays, customers demand faster customer service, and expect that organizations know them and provide appropriate services and recommendations for products quickly. Many organizations are reacting to these market needs by driving toward pervasive business intelligence, augmenting traditional business intelligence (BI) with the ability to capture, interpret and act immediately on data to make faster decisions. With pervasive business intelligence, organizations data warehouse changes to a system that can proactively and reactively interact with the business stakeholders, and it provides appropriate decision options to help marketers to respond and take action based on knowledge discovery in current integrated data. This work based on the literature review presents the Pervasive Business Intelligence state of the art and related areas, leaving open for proposing a framework to guide the development of activities of Marketing Intelligence.

关键词： Business Intelligence, Marketing Intelligence, Pervasive Business Intelligence.

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