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Barley chitinase genes expression revamp resistance against whitefly (Bemisia Tabaci) in transgenic cotton (Gossypium hirsutum L.)

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Journal of Cotton Research 2024年第1期7卷 90-100页

作者： BASHIR Samina YAQOOB Amina BASHIR Rohina BUKHARI Shanila SHAHID Naila AZAM Saira BAKHSH Allah husnain tayyab SHAHID Ahmad Ali RAO Abdul Qayyum Centre of Excellence in Molecular Biology University of the PunjabLahore 53700Pakistan Department of Botany Kinnaird College for Women UniversityLahore 54000Pakistan Department of Botany Government College UniversityFaisalabad 38000Pakistan Institute of Botany University of the PunjabLahore 54590Pakistan.

Background Chitinase is an enzyme that hydrolyzes chitin,a major component of the exoskeleton of insects,including plant pests like *** present study aimed to investigate the expression of chemically synthesized barley ch1 and chi2 genes in cotton(Gossypium hirsutum)through Agrobacterium-mediated ***-five putative transgenic cotton plants were obtained,out of which fifteen plants successfully survived and were shifted to the *** gene-specific primers,amplification of 447 bp and 401 bp fragments confirmed the presence of the ch1 and chi2 genes in five transgenic cotton plants of the T0 *** five plants were further evalu-ated for their mRNA expression *** T0 transgenic cotton plants with the highest mRNA expression level and better yield performance in field,were selected to raise their subsequent *** The T1 cotton plants showed the highest mRNA expression levels of 3.5-fold in P10(2)for the ch1 gene and 3.7-fold in P2(1)for the chi2 *** in situ hybridization(FISH)confirmed a single copy number of ch1 and chi2(hemizygous)on chromosome ***,the efficacy of transgenes on whitefly was evaluated through an insect bioassay,where after 96 h of infestation,mortality rates of whitefly were calculated to be 78%–80%in transgenic cotton *** number of eggs on transgenic cotton plants were calculated to be 0.1%–0.12 per plant compared with the non-transgenic plants where egg number was calculated to be 0.90–1.00 per *** Based on these findings,it can be concluded that the chemically synthesized barley chitinase genes(ch1 and chi2)have the potential to be effective against insects with chitin exoskeletons,including *** transgenic cotton plants expressing these genes showed increased resistance to whiteflies,resulting in reduced egg numbers and higher mortality rates.

关键词： Chitinase Cotton White fly Transgene Bioassay

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Potato Virus Y mRNA Expression Knockdown Mediated by siRNAs in Cultured Mammalian Cell Line

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Virologica Sinica 2011年第2期26卷 105-113页

作者： Bushra Tabassum Idrees Ahmad Nasir tayyab husnain National Centre of Excellence in Molecular Biology(CEMB) University of the Punjab

RNA interference(RNAi) is a powerful tool for functional gene analysis which has been successfully used to downregulate the expression levels of target *** goal of this research was to provide a highly robust and concise methodology for in-vitro screening of efficient siRNAs from a bulk to be used as a tool to protect potato plants against PVY *** our study,a 480bp fragment of the capsid protein gene of potato virus Y(CP-PVY) was used as a target to downregulate PVY mRNA expression in-vitro,as the CP gene interferes with viral uncoating,translation and replication.A total of six siRNAs were designed and screened through transient transfection assay and knockdown in expression of CP-PVY mRNA was calculated in CHO-k ***-PVY mRNA knockdown efficiency was analyzed by RT-PCR and real-time PCR of CHO-k cells co-transfected with a CP gene construct and *** biological replicates were performed in this *** our findings,one CP gene specific siRNA out of a total of six was found to be the most effective for knockdown of CP-PVY mRNA in transfected CHO-k cells by up to 80%-90%.

关键词：马铃薯Y病毒 siRNAs 细胞株哺乳动物基因表达 CP基因 RNA干扰 PCR技术

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HCV infection causes cirrhosis in human by step-wise regulation of host genes involved in cellular functioning and defense during fibrosis:Identification of bio-markers

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Genes & Diseases 2019年第3期6卷 304-317页

作者： Bushra Ijaz Waqar Ahmad Trina Das Khadija Shabbiri tayyab husnain Sajida Hassan Centre of Excellence in Molecular Biology University of the PunjabLahorePakistan School of Biological Sciences The University of QueenslandAustralia College of Medicine and Health Sciences UAE UniversityAl AinUnited Arab Emirates Division of Transplantation Department of SurgerySchool of MedicineUniversity of WashingtonSeattleWAUSA Department of Laboratory Medicine University of WashingtonSeattleWAUSA

Chronic Hepatitis C Viral(HCV)infection is a leading health problem worldwide and resulted in fibrotic scar formation,and finally *** contemporary therapies can partially reverse this destructive process,the rehabilitation is too slow and unsuitable for all chronic *** current study elucidates the mechanism of disease progression from early(F1)to moderate(F2,F3),and to severe fibrosis(F4)/cirrhosis in HCV genotype 3a infected patients to find out new candidates as potential disease progression markers and antiviral therapeutic agents.A total of 550 genes were found differentially regulated in the four fibrosis stages and grouped in 22 classes according to their biological *** set enrichment(GSEA)and Ingenuity pathway analysis(IPA)were used to identify the regulation of crucial biological functions and pathways involved in HCV *** differentially regulated the expression of genes involved in apoptosis,cell structure,signal transduction,proliferation,metabolism,cytokine signaling,immune response,cell adhesion and maintenance,and post translational modifications by pathway *** was an increasing trend of proliferative and cell growth related genes and shutting down of immune response as the disease progress mild to moderate to advanced stage *** myriad of changes in gene expression showed more chances of developing liver cancer in patients infected with HCV genotype 3a in a systematic *** identified gene set can act as disease markers for prediction,whether the fibrosis lead to cirrhosis and its association with end stage liver disease development.

关键词： Gene expression profiling Hepatitis C Liver cirrhosis Liver fibrosis Viral infection

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Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第4期12卷 326-334页

作者： Abdul Qayyum RAO Muhammad IRFAN Zafar SALEEM Idrees Ahmad NASIR Sheikh RIAZUDDIN tayyab husnain National Centre of Excellence in Molecular Biology

The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium *** and expression of PHYB gene in cotton plants were confirmed by molecular *** RNA (mRNA) expression in one of the transgenic lines,QCC11,was much higher than those of control and other transgenic *** cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal *** increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation *** recorded up to two generations,both in the greenhouse and in the field,revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,with 35% more ***,the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism,decrease in apical dominance,and increase in boll size.

关键词： Transformation Gossypium hirsutum Phytochrome B Overexpression Plant growth Yield

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Growth, Physiological and Molecular Responses of Cotton (Gossypium arboreum L.) under NaCl Stress

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American Journal of Plant Sciences 2014年第5期5卷 605-614页

作者： Sameera Hassan Muhammad Bilal Sarwar Sajjad Sadique Bushra Rashid Beenish Aftab Bahaeldeen Babiker Mohamed tayyab husnain Center of Excellence in Molecular Biology University of the Punjab Lahore Pakistan

Salinity is one of the most serious growth limiting factor, therefore, no longer being ignored. Although, cotton is fairly salt tolerant;its sensitivity at crop stand and yield is affected. This study is planned to identify the growth, physiological and molecular parameters in local cotton varieties FDH 171 and FDH 786 under NaCl stress. There was 100% seed germination but hypocotyl length was reduced at increasing level of NaCl. Plant height, fresh and dry biomass were reduced as the plants were subjected to increased stress of NaCl. Stomatal conductance, transpiration and photosynthetic rate and ionic imbalance were found to be reduced under the gradual increase in NaCl stress and affected the plant’s overall physiological processes. PCR product of AtNHX3 has been identified in stressed and non-stressed plants. Thus, the genotypes FDH 171 & FDH 786 were found tolerant to adoption of salt stress and could be used as a source in crop improvement.

关键词： Salt Stress Gossypium arboretum Cotton Gas Exchange Parameters Growth Analysis

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Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第4期7卷 291-298页

作者： RAO Abdul Qayyum HUSSAIN S. Sarfraz SHAHZAD M. Saqib BOKHARI S. Yassir Abbas RAZA M. Hashim RAKHA Allah MAJEED A. SHAHID A. Ali SALEEM Zafar husnain tayyab RIAZUDDIN S. Institution Centre of Excellence in Molecular Biology University of the Punjab Lahore 53700 Pakistan

Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypo-cotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.

关键词：愈伤组织棉花野生种细胞悬浮培养杂交

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GENE-CHIP TECHNOLOGY AND ITS APPLICATION

GENE-CHIP TECHNOLOGY AND ITS APPLICATION

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The 3rd Session of 2005 Workshop of Pakistan Academy of Sciences

作者： Muhammad Irfan tayyab husnain Penny J.Tricker Gail Taylor S.Riazuddin National Centre of Excellence in Molecular Biology 87-W Canal Bank RoadLahore-53700Pakistan School of Biological Sciences University of SouthamptonBassett Crescent EastSO16 7PXUK

Gene Chips are finding extensive use in animal and plant *** microarrays are of two kind,c DNA or oligonucleotide.c DNA microarrays were developed at Stanford University,whereas oligonucleotide were developed by *** construction of c DNA or oligonucleotide on a glass slide helps to compare the gene expression level of treated and control samples by labeling m RNA with green（Cy3） and red（Cy5） *** hybridized gene chip emit fluorescence whose intensity and colour can be *** labeling can be done directly or *** method involves amino allyle modified d UTP instead of pre-labelled *** of gene chip generally occurs in a minimum volume possible and to ensure the hetroduplex formation,a ten fold more DNA is spotted on slide than in the solutions.A confocal or semi confocal laser technologies coupled with CCD camera are used for image *** standardization,house keeping genes are used or c DNA are spotted in gene chip that are not present in treated or control ***,statistical analysis（image analysis） and cluster analysis softwares have been developed by Stanford *** gene-chip technology has many applications like expression analysis,gene expression signatures（molecular phenotypes） and promoter regulatory element co-expression.

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Genes and Transcriptional Factors in Chili Plant with Aspect to Metabolism and Resistance against Virus, Bacteria and Fungi： A Review

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Journal of Agricultural Science and Technology(B) 2014年第7期4卷 509-517页

作者： Arfan Ali Muhammad Sarmad Iftikhar Muhammad Munawar Muqadas Aleem Sajed Ali Saira Azam Idrees Ahmad Nasir tayyab husnain Umair Majid Muhammad Sameer Akram Tajasar Kamran Shehzad Bajwa Tahir Rehman Samiullah National Centre of Excellence in Molecular Biology University of the Punjab Lahore 53700 Pakistan Department of Plant Breeding and Genetics University of Agriculture Faisalabad 38000 Pakistan

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