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earthworm functional groups are related to denitrifier activity in riparian soils

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Pedosphere 2021年第3期31卷 413-422页

作者： Chen CHeN Leanne eJACK martin RCHÉNIeR Joann KWHALeN State environmental Protection Key Laboratory of Urban ecological environment Simulation and Protection South China Institute of Environmental SciencesMinistry of Ecology and Environment of ChinaGuangzhou 510535China Department of Natural Resource Sciences Macdonald CampusMcGill University21111 Lakeshore RoadSte-Anne-de-BellevueQuebec H9X 3V9Canada Department of Food Science and Agricultural Chemistry and Department of Animal Science Macdonald CampusMcGill University21111 Lakeshore RoadSte-Anne-de-BellevueQuebec H9X 3V9Canada

Riparian buffers,located in the transition zone between terrestrial and aquatic ecosystems,are a hotspot for nitrogen(N)removal through *** are abundant in riparian buffers and may enhance *** study investigated earthworm demographics of three earthworm functional groups(anecic,epigeic,and endogeic)and denitrifier activity in temporarily flooded and non-flooded riparian soils from April to October 2012 in southern Quebec,*** earthworm species,mostly endogeic,were found in the temporarily flooded soil,while only six earthworm species were found in the non-flooded *** average,there were 11.7 times more earthworms with 12.4 times greater biomass(Pe temporarily flooded soil than in the non-flooded *** denitrification enzyme activity(DeA)was of similar magnitude in temporarily flooded and non-flooded soils,with temporal variation associated with rainfall *** earthworm biomass was positively correlated(PeA,while epigeic earthworm biomass was positively correlated(Pene copies and nosZ gene copies from bacteria,indicating an association between earthworm functional groups and denitrifier activity in riparian *** multiple regressions showed that DeA in riparian soils could be predicted using soil moisture,inorganic N concentration,and earthworm functional groups,suggesting that endogeic and epigeic earthworms contributed to denitrifier activity in riparian soils.

关键词： endogeic earthwormsepigeic earthwormsriparian areasriparian buffermacrofaunaseasonal changedenitrification enzyme activitydenitrifier gene copy

Geographically distributed hybrid testing & collaboration between geotechnical centrifuge and structures laboratories

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earthquake engineering and engineering Vibration 2018年第1期17卷 53-71页

作者： Mobin Ojaghi Ignacio Lamata Martínez Matt S.Dietz martin S.Williams Anthony Blakeborough Adam J.Crewe Colin A.taylor S.P.Gopal Madabhushi Stuart K.Haigh Department of engineering Science University of Oxford Department of Civil engineering University of Bristol Department of engineering University of Cambridge

Distributed Hybrid Testing（DHT） is an experimental technique designed to capitalise on advances in modern networking infrastructure to overcome traditional laboratory capacity limitations. By coupling the heterogeneous test apparatus and computational resources of geographically distributed laboratories, DHT provides the means to take on complex, multi-disciplinary challenges with new forms of communication and collaboration. To introduce the opportunity and practicability afforded by DHT, here an exemplar multi-site test is addressed in which a dedicated fibre network and suite of custom software is used to connect the geotechnical centrifuge at the University of Cambridge with a variety of structural dynamics loading apparatus at the University of Oxford and the University of Bristol. While centrifuge time-scaling prevents real-time rates of loading in this test, such experiments may be used to gain valuable insights into physical phenomena, test procedure and accuracy. These and other related experiments have led to the development of the real-time DHT technique and the creation of a flexible framework that aims to facilitate future distributed tests within the UK and beyond. As a further example, a real-time DHT experiment between structural labs using this framework for testing across the Internet is also presented.

关键词： centrifuge distributed dynamics geotechnical hardware-in-the-loop real-time hybrid testing

An elevator Button Recognition Method Combining YOLOv5 and OCR

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计算机、材料和连续体（英文） 2023年第4期75卷 117-131页

作者： Xinliang Tang Caixing Wang Jingfang Su Cecilia taylor School of Information Science and engineering Hebei University of Science and TechnologyShijiazhuang050000China Nankai-Birmingham Institute of Data Science Intelligence BirminghamB100ABBritain

Fast recognition of elevator buttons is a key step for service robots toride elevators automatically. Although there are some studies in this field, noneof them can achieve real-time application due to problems such as recognitionspeed and algorithm complexity. elevator button recognition is a comprehensiveproblem. Not only does it need to detect the position of multiple buttonsat the same time, but also needs to accurately identify the characters on eachbutton. The latest version 5 of you only look once algorithm (YOLOv5) hasthe fastest reasoning speed and can be used for detecting multiple objects inreal-time. The advantages ofYOLOv5 make it an ideal choice for detecting theposition of multiple buttons in an elevator, but it’s not good at specific wordrecognition. Optical character recognition (OCR) is a well-known techniquefor character recognition. This paper innovatively improved the YOLOv5network, integrated OCR technology, and applied them to the elevator buttonrecognition process. First, we changed the detection scale in the YOLOv5network and only maintained the detection scales of 40 ∗ 40 and 80 ∗ 80, thusimproving the overall object detection speed. Then, we put a modified OCRbranch after the YOLOv5 network to identify the numbers on the ***, we verified this method on different datasets and compared it withother typical methods. The results show that the average recall and precisionof this method are 81.2% and 92.4%. Compared with others, the accuracyof this method has reached a very high level, but the recognition speed hasreached 0.056 s, which is far higher than other methods.

关键词： Button recognition deep learning multi-object detection

Combined effects of chitosan and microencapsulated enterococcus faecalis CG1.0007 probiotic supplementation on performance and diarrhea incidences in enterotoxigenic escherichia coli K88^+ challenged piglets

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Animal Nutrition 2017年第4期3卷 366-371页

作者： Kolawole Aluko Deepak e.Velayudhan ehsan Khafipour Aike Li Yulong Yin martin Nyachoti Department of Animal Science University of Manitoba Academy of Science and Technology of State Administration of Grain Institute of Subtropical Agriculture Chinese Academic of Sciences

The aim of this study was to investigate the combined effects of chitosan oligosaccharide(COS) and a microencapsulated enterococcus faecalis CG1.0007 probiotic(PRO) on growth performance and diarrhea incidences in enterotoxigenic escherichia coli(eTeC) K88^+ challenged piglets in a 14-d study. Thirty piglets,7.19 ± 0.52 kg initial BW weaned at 21 ± 1 *** allotted to 5 treatment groups(n = 6)consisting of a corn-soybean meal diet with no additive(negative control, NC), NC + 0.25% chlortetracycline(positive control, PC), NC + 400 mg/kg COS(COS), NC + 100 mg/kg PRO(PRO) and NC + a combination of COS and PRO(CPRO). Pigs were individually housed in cages, acclimated to treatments for a 7-d period and had ad libitum access to feed and water throughout the study, On d 8, pigs were weighed, blood samples were collected, and then orally challenged with 6 mL(1 ×10^(11) cfu/mL) of freshly grown eTeC inoculum. During post-challenge period, blood was sampled at 24 and 48 h to determine plasma urea nitrogen(PUN), and diarrhea incidences and fecal consistency scores were recorded from d 9 to 12. On d 14, all pigs were weighed and then euthanized to obtain intestinal tissue samples for histomorphometric measurements. Growth performance responses were similar among treatments during the pre-and post-challenge periods. There were no significant differences in PUN content, incidences of diarrhea, and fecal consistency scores among treatments. The intestinal histomorphology results did not differ significantly among treatments except for PC with increased(P = 0.0001) villus:crypt ratio compared with the NC. Under the conditions of the present study, it can be concluded that supplementation of piglet diets with 400 mg/kg COS, 100 mg/kg microencapsulated PRO or their combination did not significantly improve piglet growth performance both during the pre-and post-eTeC K88+ oral inoculation. Also, there were no significant reduction of incidences and severity of diarrhea after challenge

关键词： enterotoxigenic escherichia coli K88^+ Chitosan oligosaccharide enterococcus faecalis CG1 0007 probiotic Growth performance Diarrhea incidences Piglets

Spectrum and visual outcomes of Vogt-Koyanagi-Harada disease in Argentina

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International Journal of Ophthalmology(english edition) 2017年第1期10卷 98-102页

作者： Veronica e.Giordano Ariel Schlaen martin J.Guzman-Sanchez Cristobal Couto Department of Ophthalmology Inflammatory Eye Diseases ServiceHospital de Clinicas Jose de San MartinBuenos Aires C1120AARArgentina Department of Ophthalmology Oculoplastic ServiceAssociation for the Prevention of Blindness in MexicoVicente Garcia Torres 46San Lucas 04030CoyoacanMexico CityMexico

AIM： To review all cases of Vogt-Koyanagi-Harada （VKH） disease in an Inflammatory eye Disease Service in Argentina and to describe the clinical profile and outcomes of ***： The records from patients with VKH disease between January 1980 and December 2008 were retrospectively analyzed for clinical profile, complications, and treatment. Patients were classified according to their initial treatment in group 1： high corticosteroid dose [≥1 mg/（kg·d）] within 2wk of illness onset; group 2： high corticosteroid dose, 2 to 4wk of onset and group 3： patients who received the high dose after 1mo of illness onset, patients who received lower oral doses than 1 mg/（kg·d） without regarding the time of beginning of the ***： A total of 210 eyes of 105 patients were included. The mean age at presentation was 32.6±13y （range： 10-74y）, and 86.7% were female. The mean duration of follow up was 144±96.6mo. Patients in the group 1 had significantly higher visual acuity than the other groups （P〈0.0001）, none had （loss of, or no） light perception at the end of follow up, whereas 24.7% patients in group 3 ended in light perception （P〈0.004）.CONCLUSION： Patients with early high dose corticosteroid treatment have better visual acuity and fewer complications. Proper timing in referral and treatment is critical for better visual outcome in VKH disease.

关键词： Vogt-Koyanagi-Harada disease Argentine population clinical spectrum treatment

基于系数匹配与参数整合的P波旅行时计算方程

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吉林大学学报（地球科学版） 2024年第1期54卷 337-344页

作者：魏建孙祥娥长江大学电子信息学院湖北荆州434023

基于泰勒展开的旅行时公式是计算P波旅行时的一种常用方法。泰勒展开的结果仅在计算点附近准确,而地层中具有垂直对称轴的横向各向同性(transverse isotropy with vertical symmetry axis,VTI)介质进一步放大了旅行时计算的不准确性。... 详细信息

基于泰勒展开的旅行时公式是计算P波旅行时的一种常用方法。泰勒展开的结果仅在计算点附近准确,而地层中具有垂直对称轴的横向各向同性(transverse isotropy with vertical symmetry axis,VTI)介质进一步放大了旅行时计算的不准确性。本文提出了一种改进的P波旅行时计算方法。该方法以基于泰勒展开的偏移距八阶旅行时方程为基础,利用系数匹配方法分别处理偏移距八阶项与六阶项的系数、偏移距六阶项与四阶项的系数,用整合处理优化得到的参数结构,形成了基于系数匹配与参数整合的偏移距四阶P波旅行时计算方法。水平层状VTI介质模型测试结果表明,该方法能够得到更小的计算误差,保证其在远偏移距处具有较好的表现。三维垂直地震剖面数据实验结果表明,该方法经参数调整后能够进行数据分析,在中偏移距处的表现相对稳定,在远偏移距处具有一定优势。

关键词：旅行时计算系数匹配参数整合 VTI介质

Laser capture microdissection and genetic analysis of carbon-labeled Kupffer cells

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World Journal of Gastroenterology 2009年第14期15卷 1708-1718页

作者： Stephan Gehring edmond Sabo Maryann e San martin elizabeth M Dickson Chao-Wen Cheng Stephen H Gregory Department of Medicine Rhode Island Hospital and The Warren Alpert Medical School of Brown University Providence RI 02903 United States Department of Pathology Rhode Island Hospital and The Warren Alpert Medical School of Brown University Providence RI 02903 United States zentrum fur Kinder- und Jugendmedizin der Universitaitsmedizin der Johannes Gutenberg-Universitait 55101 Mainz Germany

AIM： To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection （LCM）. MeTHODS： Tissues are complex structures comprised of a heterogeneous population of interconnected cells. LCM offers a method of isolating a single cell type from specific regions of a tissue section. LCM is an essential approach used in conjunction with molecular analysis to study the functional interaction of cells in their native tissue environment. The process of labeling and acquiring cells by LCM prior to mRNA isolation can be elaborate, thereby subjecting the RNA to considerable degradation. Kupffer cell labeling is achieved by injecting India ink intravenously, thus circumventing the need for in vitro staining. The significance of this novel approach was validated using a cholestatic liver injury model. ReSULTS： mRNA extracted from the microdissected cell population displayed marked increases in colonystimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer ceils derived from bile-duct-ligated, versus sham-operated, mice was compared. Microarray analysis revealed a significant （2.5-fold, q value 〈 10） change in 493 genes. Based on this fold-change and a standardized PubMed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury. CONCLUSION; The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.

关键词： Kupffer cells India ink Laser capturemicrodissection Bile duct ligation DNA microarray

Facile Synthesis of Naphtho[2,3-dJthiazoles, Naphtho[2,3-e]-[1,3,4]thiadiazines and Bis（naphtho[2,3-d]thiazolyl）copper（ll） Derivatives from Heteroylthiosemicarbazides

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Chinese Journal of Chemistry 2016年第8期34卷 814-822页

作者： Alaa A. Hassan Nasr K. Mohamed Lamiaa e. Abd eI-Haleem Stefan Brase.martin Nieger Chemistry Department Faculty of Science Minia University 61519 EI-Minia A. R. Egypt Institute of Organic Chemistry Karlsruhe Institute of Technology Fritz-Haber-Weg 6 76131 Karlsruhe Germany Laboratory of Inorganic Chemistry Department of Chemistry University of Helsinki P.O.Box55 (A. I. Virtasenaukio 1) 00014 Helsinki Finland

A one step synthesis protocol for the conversion of heteroylthiosemicarbazides and 2,3‐dichloro‐1,4‐naphthoquinone to naphtho[2,3‐ d ]thiazoles, naphtho[2,3‐ e ][1,3,4]thiadiazines as well as bis(naphtho[2,3‐ d ]thiazolyl)copper(II) derivatives is described. The products were conclusively confirmed by single crystal X‐ray analyses. A mechanism for the formation of the products is presented.

关键词： heteroylthiosemicarbazides 2,3‐dichloro‐1,4‐naphthoquinone naphtho[2,3‐d]thiazoles naphtho[2,3‐e]thiadiazines bis(naphtho[2,3‐d]thiazolyl)copper(II) derivatives

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