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Human bone graft cytocompatibility with mesenchymal stromal cells is comparable after thermal sterilization and washing followed byγ-irradiation:an in vitro study

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Regenerative Biomaterials 2018年第2期5卷 85-92页

作者： Dmitry Labutin Konstantin Vorobyov Svetlana Bozhkova Ekaterina Polyakova tatyana Vodopyanova Division of Wound Infection Treatment and Prevention Vreden Russian Research Institute of Traumatology and OrthopedicsSt.PetersburgRussian Federation

Human bone allografts present a better alternative to autografts in terms of minimization of the harvesting procedure *** to the use in clinical applications,they require sterilization which aims to reduce *** often comes at the expense of their biological properties as carriers of *** this study,we evaluated the cytocompatibility of human bone allografts processed and sterilized by three different methods withmesenchymal stromal *** morphology,biological and biochemical properties of the extracted bone-conditionedmedium and viability of cells were *** found that chemical sterilization had a strong negative effect on cell viability,whereas thermal sterilization and washing with subsequentγ-irradiation both resulted in a bone graft compatible with the progenitor ***,washing of the bone prior to sterilization allowed solid removal of cell debris and other bone marrow *** together,our findings demonstrate the importance of a proper choice of the bone graft processing method for the production of the biomaterial suitable for tissue engineering.

关键词： bone grafts bone sterilization mesenchymal stromal cells biocompatibility testing

Benefits of Ilizarov automated bone distraction for nerves and articular cartilage in experimental leg lengthening

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World Journal of Orthopedics 2017年第9期8卷 688-696页

作者： Nathalia Shchudlo tatyana Varsegova tatyana Stupina Michael Shchudlo Marat Saifutdinov Andrey Yemanov Laboratory of Morphology FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To determine peculiarities of tissue responses to manual and automated Ilizarov bone distraction in nerves and articular *** Twenty-nine dogs were divided in two experimental groups: Group M-leg lengthening with manual distraction(1 mm/d in 4 steps), Group A-automated distraction(1 mm/d in 60 steps) and intact group. Animals were euthanized at the end of distraction, at 30 th day of fixation in apparatus and 30 d after the fixator removal. M-responses in gastrocnemius and tibialis anterior muscles were recorded, numerical histology of peronealand tibialis nerves and knee cartilage semi-thin sections, scanning electron microscopy and X-ray electron probe microanalysis were *** Better restoration of M-response amplitudes in leg muscles was noted in A-group. Fibrosis of epineurium with adipocytes loss in peroneal nerve, subperineurial edema and fibrosis of endoneurium in some fascicles of both nerves were noted only in M-group, shares of nerve fibers with atrophic and degenerative changes were bigger in M-group than in A-group. At the end of experiment morphometric parameters of nerve fibers in peroneal nerve were comparable with intact nerve only in A-group. Quantitative parameters of articular cartilage(thickness, volumetric densities of chondrocytes, percentages of isogenic clusters and empty cellular lacunas, contents of sulfur and calcium) were badly changed in M-group and less changed in *** Automated Ilizarov distraction is more safe method of orthopedic leg lengthening than manual distraction in points of nervous fibers survival and articular cartilage arthrotic changes.

关键词： Limb lengthening Articular cartilage Nerve Histomorphometry Dog

Endoparasites of the Siberian tiger(Panthera tigris altaica)

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Integrative Zoology 2018年第5期13卷 507-516页

作者： tatyana V.MOSKVINA Michail Yu.SCHELKANOV Mariya A.BEGUN Far Eastern Federal University VladivostokPrimorsky KraiRussia Federal Scientific Center of Terrestrial Biodiversity of Eastern Asia Far Eastern Branch of Russian Academy of SciencesVladivostokPrimorsky KraiRussia

There have been few reports on the diversity and prevalence of parasitic fauna of the endangered Siberian tiger,which inhabits the territory of the Russian Far *** present review attempts to summarize the information about the parasitic fauna of wild Siberian tigers,which includes 15 helminths and 3 protozoan *** most prevalent parasitic species was found to be Toxocara cati,followed by Toxascaris *** commonly recorded Platyhelminth species is Paragonimus westermani,which causes a lethal infection of the lung parenchyma in Siberian ***,the information about infections by this fluke in the Siberian tigers is scarce,although *** infections pose a serious health hazard to tiger *** nematodes Aelurostrongylus abstrusus and Thominx aerophilus are found in Siberian tigers with an occurrence rate of 2.3%and 19%,*** information on the parasitic infestations of captive populations of Siberian tigers is also presented along with the sources of infection and hazards for the wild tiger populations in their natural environment.

关键词： helminths infection parasites protozoan Siberian tiger

Monitoring cellular metabolism of 3T3 upon wild type E. coli infection by mapping NADH with FLIM

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作者： tatyana Tolstaya

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Chinese Optics Letters 2010年第10期8卷 931-933页

作者： tatyana Buryakina Pin-Tzu Su Vladimir Gukassyan Wan-Jr Syu Fu-Jen Kao Institute of Biophotonics National Yang-Ming University Institute of Microbiology and Immunology National Yang-Ming University Department of Photonics National Sun Yat-Sen University

Fluorescence lifetime imaging microscopy （FLIM） has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide （NADH）, one of the main compounds of oxidative phosphorylation. In this letter, we apply the technique to characterize the metabolic changes in mouse embryonic fibroblast 3T3 cells upon bacterial infection. A gradual shortening of the decaying time constants in both the short and the long lifetime components of NADH＇s autofluorescence is detected. The ratio of the short and the long lifetime components＇ relative contributions, however, shows a rapid increase, indicating the rise of cellular metabolic activity over the course of infection.

关键词： Amides Cell culture Phosphorylation Tissue

Structure-activity relationship in water-gas shift reaction over gold catalysts supported on Y-doped ceria

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Journal of Rare Earths 2019年第4期37卷 383-392页

作者： tatyana Tabakova Lyuba Ilieva Ivan Ivanov Maela Manzoli Rodolfo Zanella Petya Petrova Zbigniew Kaszkur Institute of Catalysis Bulgarian Academy of Sciences Department of Drug Science and Technology & Centre for Nanostructured Interfaces and Surfaces University of Turin Centro de Ciencias Aplicadas y Desarrollo Tecnologico Universidad Nacional Autonoma de Mexico Institute of Physical Chemistry PAS

The utilization of pure hydrogen as an energy source in fuel cells gave rise to renewed interest in developing active and stable water-gas shift catalysts. Gold catalysts have proven to be very efficient for water-gas shift reaction at low temperature. The aim of the present study was to investigate the effect of:(i) different preparation methods(impregnation and coprecipitation) to obtain a modified ceria support,and(ii) the amount of Y_2 O_3(1.0 wt%, 2.5 wt%, 5.0 wt% and 7.5 wt%) as dopant on the water-gas shift activity of Au/CeO_2 catalysts. An extended characterization by means of S_(BET), XRD, HRTEM/HAADF, FTIR,H_2-TPR and CO-TPR measurements in combination with careful evaluation of the catalyst behavior allowed to shed light on the parameters governing the water-gas shift activity. The catalysts show very high activity(>90% CO conversion) in the temperature range 180-220 ℃,with a slightly better performance of the gold catalysts on supports prepared by impregnation. The decreased activity with increasing Y_2 O_3 concentration is related to the hindering of oxygen mobility due to ordering of surface oxygen vacancies in vicinity of segregated Y^(3+). The effect of catalyst pre-treatments and the stability of the best performing samples were examined as well.

关键词： Gold catalyst Water gas shift reaction Doped ceria Yttrium Hydrogen production Rare earths

Assessment of palmar subcutaneous tissue vascularization in patients with Dupuytren's contracture

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World Journal of Orthopedics 2018年第9期9卷 130-137页

作者： Nathalia Shchudlo tatyana Varsegova tatyana Stupina Tamara Dolganova Michael Shchudlo Nathalia Shihaleva Vadim Kostin Clinics and Experimental Laboratory for Reconstructive Microsurgery and Hand Surgery Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics" Laboratory of Morphology of Federal State Budget Institution Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different *** Eighty-seven Dupuytren's contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old(Y-group, n = 22); the others were 55 and older(O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the *** Frequency of cases with rapid development of contracture(less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P < 0.05. The external and luminal diameters of perforating arteries in palmar fascia were decreased more severely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels(more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with *** Compared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.

关键词： Dupuytren’s contracture Laser Doppler flowmetry Hypodermis Histo-morphometry Palmar fascia

Influence of 10-(6-plastoquinonyl) decyltriphenylphosphonium on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced hyperglycemia

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World Journal of Diabetes 2019年第12期10卷 546-559页

作者： Aleksander A Agarkov tatyana N Popova Yana G Boltysheva Department of Medical Biochemistry and Microbiology Voronezh State University

BACKGROUND It is known that under conditions of tissue tolerance to insulin,observed during type 2 diabetes mellitus(DM2),there is an increased production of reactive oxygen ***,the free radicals can initiate lipid peroxidation(LPO)in lipoprotein *** concentration of LPO products can influence the state of insulin receptors,repressing their hormone connection activity,which is expressed as a reduction of the glucose consumption by *** is possible that reduction in glucose concentration during administration of 10-(6-plastoquinonyl)decyltriphenylphosphonium(SkQ1)to rats with DM2 may be related to the antioxidant properties of this *** To establish the influence of SkQ1 on free-radical homeostasis in the heart and blood serum of rats with streptozotocin-induced *** To induce hyperglycemia,rats were fed a high-fat diet for 1 mo and then administered two intra-abdominal injections of streptozotocin with a 7-d interval at a 30 mg/kg of animal weight dose with citrate buffer equal to pH ***1 solution was administered intraperitoneally at a 1250 nmol/kg dose per *** samples were taken from control animals,animals with experimental hyperglycemia,rats with streptozotocin-induced glycemia that were administered SkQ1 solution,animals housed under standard vivarium conditions that were administered SkQ1,rats that were administered intraperitoneally citrate buffer equal to pH 4.4 once a week during 2 wk after 1-mo high-fat diet,and animals that were administered intraperitoneally with appropriate amount of solution without SkQ1(98%ethanol diluted eight times with normal saline solution).To determine the intensity of free radical oxidation and total antioxidant activity,we used the biochemiluminescence *** hydratase(AH),superoxide dismutase,and catalase activities were estimated using the Hitachi U-1900 spectrophotometer supplied with *** amount of citrate was determined by means of the Natel

关键词： Diabetes mellitus Free radical oxidation Antioxidants 10-(6-plastoquinonyl)decyltriphenylphosphonium

Electron probe microanalysis оf experimentally stimulated osteoarthrosis in dogs

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World Journal of Orthopedics 2017年第9期8卷 681-687页

作者： tatyana Stupina Michael Shchudlo Michael Stepanov Laboratory of Morphology FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To develop methods of articular cartilage preparation for X-ray-electron probe microanalysis and to study its elements content in experimental *** Twenty dogs aged 2-8 years were divided in research(aged 2 years, induction of osteoarthrosis-IOA) and intact group. Intact group included three subgroups(aged 2, 5 and 8 years). Samples of cartilage after araldite saturation and pouring were partially cut into semithin sections stained with methylene blue and with methylene blue-basic fuchsin. Their smooth surfaces were investigated by X-ray-electron probe microanalysis. Spatial distribution of sulfur, calcium and phosphorus and their concentrations(weight %) were *** X-ray electron probe microanalysis revealed non-uniformsulfur distribution in cartilage of intact animals: Its content increases from superficial zone to deep one, this regularity was preserved in animals with IOA. Differences of IOA with spontaneous chondropathy were revealed. Spontaneous aging was characterized by calcium and phosphorus storage in deep and calcified zones and compensatory increase of sulfated glycosaminoglycans in intermediate and deep cartilage zones as evidenced by the metachromatic reaction and microanalysis data. Unlike spontaneous chondropathy connected with aging in experimentally stimulated osteoarthrosis more intensive storage of calcium but minor phosphorus in intermediate zone were marked. In IOA the calcified cartilage thinning and osteoclastic resorption are apparent with few changes of elements composition; the only difference from control is minority phosphorus *** The obtained results demonstrate specific tricks of X-ray electron probe microanalysis and its possibility in the research of mechanisms of articular cartilage alterations in osteoarthrosis.

关键词： Osteoarthrosis Articular cartilage Dog X-ray electron probe microanalysis