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Carbon-Ion Beams Efficiently Induce Cell Killing in X-Ray Resistant Human Squamous Tongue Cancer Cells

International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2014年第3期3卷 133-142页

作者： Akihisa Takahashi Hongyu Ma Akiko Nakagawa Yukari Yoshida Tatsuaki Kanai tatsuya Ohno Yoshikazu Kuwahara Manabu Fukumoto Takashi Nakano Advanced Scientific Research Leaders Development Unit Gunma University Gunma Japan Department of Pathology IDAC Tohoku University Sendai Japan Department of Radiation Oncology Gunma University Graduate School of Medicine Gunma Japan Gunma University Heavy Ion Medical Center Gunma Japan

In order to see whether carbon ion (C-ion) beams have a biological advantage over X-rays, studies were designed to examine the effects of C-ion beams on radiosensitivity in X-ray resistant cells. Clinically relevant X-ray resistant SAS-R cells derived from human tongue cancer SAS cells were used. The cells were exposed to X-rays or Spread-Out Bragg peak (SOBP) beam C-ions. Cell survival was measured using a modified high-density survival assay. Cell survival signaling and cell death signaling were analyzed using flow cytometry. The cells were labeled with putative cancer stem cell markers such as CD44 and CD326. SAS-R cells were 1.6 times more radioresistant than SAS cells after exposure to X-rays. Cell survival was similar in each cell line after exposure to C-ion beams. SAS-R cells displayed enhanced cell survival signaling when compared to SAS cells under normal conditions. On the other hand, the phosphorylation of AKT-related proteins decreased and polycaspase activities were enhanced when cells were irradiated with C-ion beams in both cell lines. More CD44 and CD326 positive cells were seen in SAS-R cells than in SAS cells. Moreover, the marker positive cell numbers significantly decreased after exposure to C-ion beams when compared to X-rays at iso-survival doses in SAS-R cells. C-ion beams efficiently induced cell killing in X-ray resistant cells which displayed activated cell survival signaling and contained more numerous cancer stem-like cells.

关键词： Carbon-Ion Beams X-Ray Resistant Cells Cell Survival and Death Signaling Cancer Stem-Like Cells

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Lung cancer biopsy dislodges tumor cells into circulating blood

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Journal of Cancer Metastasis and Treatment 2017年第1期3卷 16-20页

作者： Noriyoshi Sawabata Tomotaka Kitamura Yuko Nitta Tomoyo Taketa Takeshi ohno tatsuya Fukumori Takeru Hyakutake Takahito Nakamura Division of General Thoracic Surgery Respiratory CenterHoshigaoka Medical CenterHirakataOsaka 574-8511Japan.

Aim:A“seed”of lung cancer metastasis is circulating tumor cells(CTCs),which may be dislodged from a tumor during biopsy.This possibility was assessed among patients who underwent lung tumor biopsy using flexible fiber-topic bronchoscopy(FFB).Methods:The study involved six patients with non-small cell lung cancer who underwent FFB biopsy to diagnose a lesion pathologically(5 males and 1 female,median age 63 years,6 adenocarcinomas,of 4 clinical-stage IA,1 stage IB,and 1 stage IIIA),CTCs were extracted from the peripheral vein blood at pre-FFB and at post-FFB using a size selection method.Results:No t umor c ell was d etected a t p re-a nd p ost-FFB was i n t hree c ases(50%);no tumor cells were detected pre-FFB while CTCs were detected at post-FFB in two cases(33.3%);and CTCs were detected at pre-FFB with numerous CTCs detected at post-FFB in one case(17.7%).In addition,similar tendencies were observed in each analysis of single-cell and clustered-cell categories.Conclusion:These results suggest that a FFB biopsy of lung cancer may potentially dislodge CTCs from a tumor into the circulating peripheral blood.

关键词： Lung cancer biopsy circulating tumor cells

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Development of Nursing Protocol for Preventing Discontinuation of Treatments by Methods Other than Physical Restraint during Acute Exacerbation of Chronic Heart Failure in Patients with Impaired Cognitive Function

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Health 2018年第6期10卷 789-815页

作者： Haruka Otsu Shiori Fujimoto Nozomi Murakami Tatsuki Ohhara Yoko Takeya tatsuya Ohno Chieko Suzuki Sanae Takahashi Graduate School of Health Sciences Hirosaki University Hirosaki City Japan Japan Community Health Care Organization Shiga Hospital Shiga Japan Akita Prefecture Health Agricultural Cooperative Association Hiraka General Hospital Yokote Japan Akita Prefecture Health Agricultural Cooperative Association Koto Kosei Hospital Hachirogata Japan Aomori Prefectural Central Hospital Aomori Japan Medical Corporation Association Seikokai Hirano Synthesis Hospital Gifu Japan Ugo Municipal Hospital Ugo Japan Omagari Nakadori Hospital Omagari Japan

The purpose of this study is to prepare a nursing protocol for preventing discontinuation of treatments using a method other than physical restraint during acute exacerbation of chronic heart failure in patients with impaired cognitive function. For the first stage of the study, we prepared a draft of the nursing protocol based on a basic survey. For the second stage, semi-structured interviews were conducted with 5 nurses specialized in chronic heart failure and 10 nurses in dementia case to ensure content validity of the draft protocol. For the third stage, we examined the possibility of clinical application of the revised version of the protocol draft prepared in the second stage of the study. For assessment items, significant points of nursing care, and specific nursing care practice in this revised version, 154 subjects (93.9%) considered effective for patients, in terms of prevention of treatment discontinuation using a method other than physical restraint. All items and contents were considered useful by more than 60% of the nurses. Considering that the nurses working in the clinical setting reported 93.9% of usefulness, we concluded that this nursing protocol remained valid at a certain level. We also received a comment from the certified nurses that we should include the basic contents for newly graduated nurses. We consider that this nursing protocol will be also useful for newly graduated nurses to acquire knowledge. It helped to standardize nursing care in order to predict potential risks for patients with impaired cognitive function.

关键词： Dementia Impaired Cognitive Function Acute Exacerbation of Chronic Heart Failure Physical Restraint Nursing Protocol

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