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Bioinformatic analysis of the ssc-miR-146b upstream promoter region

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Animal Nutrition 2015年第4期1卷 368-372页

作者： Xin tao xiaoming Men Ziwei Xu Institute of Animal Husbandry and Veterinary Science Zhejiang Academy of Agricultural Sciences

Sus Scrofa microRNA-146b-5p(ssc-miR-146b) was found to be one of differentially expressional microRNAs(miRNA) in our previous study. Not only it is highly expressed but also it maintains the largest up-regulated differences on the expressional level at different time points in the small intestinal mucosa of weaned piglets. To further explore the regulation mechanism of microRNA-146b-5 p(miR-146b)during the stressful progress in weaned piglets, the present study predicted the functions of the ssc-miR-146b upstream promoter region using biological analysis. The analytical results showed that ssc-miR-146b is an intergenic miRNA. The length of the promoter region of ssc-miR-146b was predicted to be2,249 bp using the Ensemble database. The length of the CpG island in the ssc-miR-146b promoter region was found to be 167 bp and it was located from 464 to 630 bp. Twenty six binding sites of 9 transcription factors in the upstream promoter region, including the sites of genes such as Spl, AP-1, MyoD, GATA etc,were discovered using different kinds of analytical software. The predictions of the CpG island and transcription factor binding sites provided significant information for further studying the transcriptional regulation mechanism of ssc-miR-146b on the small intestinal injury due to weaning stress.

关键词： microRNA-146b-5p Bioinformatic analysis Promoter Swine Intestine

激光工艺参数对铝合金烧蚀特征尺寸的影响研究

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机械工程师 2024年第2期 132-135,139页

作者：梁民徐涛王梦华李晓明航空工业西安航空计算技术研究所西安710068

相较于传统的人工标记、喷墨标记、电脑刻字标记等对产品进行唯一性标记的方式,激光标记具有速率快、加工精度高、热影响区小且经济实惠的特点,从而具有较大的实用价值。而在激光标记过程中,工艺参数选择不当容易造成标记不清晰,难以识... 详细信息

相较于传统的人工标记、喷墨标记、电脑刻字标记等对产品进行唯一性标记的方式,激光标记具有速率快、加工精度高、热影响区小且经济实惠的特点,从而具有较大的实用价值。而在激光标记过程中,工艺参数选择不当容易造成标记不清晰,难以识别的问题。文中通过数值模拟与试验研究激光工艺参数与烧蚀特征尺寸之间的关系,结论如下:激光功率、激光频率及脉冲宽度与烧蚀深度、宽度成正比,然而激光功率、频率过大,会产生难以消除的多余物,在实际应用中推荐使用激光功率60 W、脉冲频率40 k Hz、脉冲宽度500 ns作为激光标记参数。

关键词：激光标记烧蚀特征激光工艺参数

不同体质量指数老年2型糖尿病患者血糖波动的特点分析

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老年医学与保健 2023年第2期29卷 284-289页

作者：张菁黄宇新陈芳陶晓明蒋翠萍孙皎谭雯复旦大学附属华东医院内分泌科上海200040

目的通过连续血糖监测方法,分析不同体质量指数(BMI)的老年2型糖尿病患者血糖波动特点。方法回顾性分析2021年于复旦大学附属华东医院内分泌科住院、65岁及以上并行动态血糖监测的146例2型糖尿病患者,所有患者均记录人口统计学及实验... 详细信息

目的通过连续血糖监测方法,分析不同体质量指数(BMI)的老年2型糖尿病患者血糖波动特点。方法回顾性分析2021年于复旦大学附属华东医院内分泌科住院、65岁及以上并行动态血糖监测的146例2型糖尿病患者,所有患者均记录人口统计学及实验室指标等数据。按照BMI将患者分为3组,体重正常组BMI<24 kg/m^(2),超重组BMI 24~28 kg/m^(2)之间,肥胖组的BMI≥28 kg/m^(2)。比较不同BMI患者在年龄、性别、化验指标、血糖波动、用药及病程等方面的差异。在亚组中检测体重正常和超重组+肥胖组患者血清成纤维细胞生长因子19(FGF19)水平。结果 (1)3组患者病程、年龄、糖化血红蛋白、空腹血糖及餐后2 h血糖差异无统计学意义。体重正常组空腹及餐后2 h C肽水平低于超重及肥胖组,最低血糖从体重正常组到超重组再到肥胖组,逐渐递增。在血糖波动的参数上,平均血糖水平、葡萄糖目标范围内时间在3组之间差异无统计学意义;而日内血糖波动最大幅度从体重正常组到超重组再到肥胖组,逐渐递减。体重正常组黎明现象发生率高于另外2组。BMI与最低血糖呈正相关(r=0.187,P=0.024)、与日内血糖波动最大幅度呈负相关(r=-0.222,P=0.007)。(2)易发生低血糖风险的药物如胰岛素、磺脲类使用率在3组之间差异无统计学意义,而二甲双胍及DPP4-i使用率在3组间差异有统计学意义,超重组及肥胖组二甲双胍及DPP4-i使用率均高于体重正常组。(3)在亚组中检测体重正常组和超重+肥胖组血清FGF19水平,发现体重正常组FGF19水平较超重+肥胖组显著升高。结论体重正常的老年2型糖尿病患者夜间血糖偏低、血糖波动大,偏低的血糖受降糖药物影响小,可能受FGF19影响。

关键词：老年连续血糖监测 2型糖尿病体质量指数

松辽盆地北部深层火山岩气藏勘探开发关键技术

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大庆石油地质与开发 2024年第3期43卷 172-182页

作者：高涛曹宝军纪学雁刘家军李伟毕晓明徐岩狄嘉祥唐亚会中国石油大庆油田有限责任公司勘探开发研究院黑龙江大庆163712

松辽盆地北部徐家围子断陷火山岩气藏是徐深气田的主力气藏,具有岩性多样、储层非均质性强的特点,面临气藏富集规律不清、优质储层预测难、气井产能和井控储量变化规律复杂、剩余气挖潜难度大等一系列技术难点和挑战。通过开展火山岩气... 详细信息

松辽盆地北部徐家围子断陷火山岩气藏是徐深气田的主力气藏,具有岩性多样、储层非均质性强的特点,面临气藏富集规律不清、优质储层预测难、气井产能和井控储量变化规律复杂、剩余气挖潜难度大等一系列技术难点和挑战。通过开展火山岩气藏成藏演化过程分析、火山岩喷发期次描述、有效储层预测、井控储量评价、剩余潜力描述等工作,攻关形成了火山岩气藏成藏评价、三级火山体精细刻画、动态储量评价、剩余潜力评价及挖潜等技术。建立了“低渗增长型+高渗稳定型”2类动态储量模式及动静结合“六参数”挖潜技术政策界限,落实了烃源岩生气期及气藏成藏期,明确了气藏富集受源岩、断裂、储层、盖层四因素的联合控制,实现了对火山岩储层内部结构的精细表征,搞清了2类气井动态储量增长规律,形成了不同类型剩余气的挖潜技术对策。基于火山岩气藏一系列勘探开发关键技术的创新,徐深气田的天然气产量逐年攀升,形成的系列技术在大庆油田已得到全面推广应用,并取得了明显效果,有效支撑了大庆油田天然气产量的持续稳定增长。研究成果可为同类型气藏的勘探开发提供有效的技术指导。

关键词：火山岩气藏富集规律火山体刻画动态储量剩余气挖潜技术松辽盆地

Enrichment of beneficial cucumber rhizosphere microbes mediated by organic acid secretion

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Horticulture Research 2020年第1期7卷 662-674页

作者： tao Wen Jun Yuan xiaoming He Yue Lin Qiwei Huang Qirong Shen Jiangsu Provincial Key Lab for Organic Solid Waste Utilization Key Laboratory of Plant ImmunityJiangsu Collaborative Innovation Center for Solid Organic WastesEducational Ministry Engineering Center of Resource-saving FertilizersNanjing Agricultural University210095 NanjingChina Vegetable Research Institute Guangdong Academy of Agricultural SciencesGuangzhou510640 GuangdongChina

Resistant cultivars have played important roles in controlling Fusarium wilt disease,but the roles of rhizosphere interactions among different levels of resistant cultivars are still ***,two phenotypes of cucumber,one resistant and one with increased susceptibility to Fusarium oxysporum ***(Foc),were grown in the soil and hydroponically,and then 16S rRNA gene sequencing and nontargeted metabolomics techniques were used to investigate rhizosphere microflora and root exudate *** high microbial community evenness for the Foc-susceptible cultivar was detected,and the relative abundances of Comamonadaceae and Xanthomonadaceae were higher for the Foc-susceptible cultivar than for the other *** analysis revealed that specific functional traits,such as protein synthesis and secretion,bacterial chemotaxis,and small organic acid metabolism pathways,were significantly upregulated in the rhizobacterial community of the Foc-susceptible cultivar.A machinelearning approach in conjunction with FishTaco plus metabolic pathway analysis revealed that four organic acids(citric acid,pyruvate acid,succinic acid,and fumarate)were released at higher abundance by the Foc-susceptible cultivar compared with the resistant cultivar,which may be responsible for the recruitment of Comamonadaceae,a potential beneficial microbial *** validation demonstrated that Comamonadaceae can be“cultured”by these organic ***,compared with the resistant cultivar,the susceptible cucumber tends to assemble beneficial microbes by secreting more organic acids.

关键词： group susceptibility synthesis

Design and experimental performance verification of a thermal property test-bed for lunar drilling exploration

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Chinese Journal of Aeronautics 2016年第5期29卷 1455-1468页

作者： Zhang tao Zhao Zeng Liu Shuting Li Jinglin Ding Xilun Yin Shen Wang Guoxin Lai xiaoming School of Mechanical Engineering and Automation Beihang University Beijing 100083 China China Academy of Space Technology Beijing 100094 China

Chinese Chang＇e lunar exploration project aims to collect and return subsurface lunar soil samples at a minimum penetration depth of 2 m in 2017. However, in contrast to those on the Earth, automated drilling and sampling missions on the Moon raise the risk of burning bits. Test-beds are required for testing the thermal properties of drill tools in a lunar environment. In this paper, a novel temperature measuring method based on thermocouples and a slip ring was proposed. Furthermore, a data acquisition system for a drilling process was designed. A vacuous, cryogenic, and anhydrous soil environment simulating the lunar surface was established. A drilling test-bed that can reach a depth of 2.2 m was developed. A control strategy based on online monitoring signals was proposed to improve the drilling performance. Vacuum and non-vacuum experiments were performed to test the temperature rising effect on drill tools. When compared with the non-vacuum experiment, the vacuum temperature rise resulted in a 12 ℃ increase. These experimental results provide significant support for Chinese lunar exploration missions.

关键词： Drilling test bed Lunar drill Lunar environment simulator Temperature measurement Thermal property

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四种红豆属植物耐旱性综合评价

浙江农业学报 2024年第2期36卷 308-324页

作者：田晓明向光锋牟村吕浩马涛朱路彭静张敏何艳湖南省植物园湖南长沙410116

为探究软荚红豆(Ormosia semicastrata)、花榈木(Ormosia henryi)、木荚红豆(Ormosia xylocarpa)和鄂西红豆(Ormosia hosiei)等4种红豆属植物在干旱胁迫与复水处理条件下的生理响应机制和种间耐旱性差异,采用盆栽控水试验法进行干旱胁... 详细信息

为探究软荚红豆(Ormosia semicastrata)、花榈木(Ormosia henryi)、木荚红豆(Ormosia xylocarpa)和鄂西红豆(Ormosia hosiei)等4种红豆属植物在干旱胁迫与复水处理条件下的生理响应机制和种间耐旱性差异,采用盆栽控水试验法进行干旱胁迫试验。试验分别设置了对照组(CK)、轻度干旱组(LD)、中度干旱组(MD)和重度干旱组(SD)4个处理组,干旱胁迫28 d后进行复水处理,分别于第0、7、14、21、28、42天(复水后第14天)测定植物形态、生理和光合特性等指标的变化,利用主成分分析法和隶属函数分析法对4种植物的耐旱性进行综合评价。结果表明,SD处理组4种红豆属植物均出现明显的旱害症状,木荚红豆旱害症状出现最早也最严重,鄂西红豆则表现最轻。4种植物生理指标随干旱胁迫程度增加和胁迫时间延长,叶片相对含水量(RWC)呈降低趋势,电导率(REC)和丙二醛(MDA)含量则逐渐升高,超氧化物歧化酶(SOD)活性、可溶性蛋白(SP)和叶绿素含量(CHL)呈先升后降趋势,叶片净光合速率(P_(n))、气孔导度(G_(s))和蒸腾速率(T_(r))逐渐降低,胞间CO_(2)浓度(Ci)呈先降后升趋势。复水处理14 d后,除木荚红豆SD处理组植株死亡无法恢复外,4种红豆属植物各处理组出现的旱害症状均得到缓解;同时,RWC、SOD活性、SP和CHL含量、P_(n)、G_(s)、T_(r)呈上升趋势,而REC、MDA含量呈下降趋势,各树种SD处理组指标变化最为显著。通过主成分分析法筛选出REC、P_(n)、G_(s)和T_(r)为4种红豆属植物耐旱性评价关键指标;通过隶属函数分析法综合评价4种红豆属植物的耐旱能力,从强到弱排序依次为:鄂西红豆、花榈木、木荚红豆、软荚红豆。研究结果可为红豆属植物的保护、耐旱性育种和栽培应用提供理论依据。

关键词：红豆属干旱胁迫生理响应复水综合评价

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正常范围内轻度升高的丙氨酸转氨酶与2型糖尿病患者夜间血糖升高的相关性

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上海医学 2019年第4期42卷 193-197页

作者：黄宇新陶晓明蒋翠萍邱婕萸真谭雯冯强保志军汪海东顾芹孙皎复旦大学附属华东医院内分泌科上海200040 复旦大学附属华东医院消化内科上海200040

目的探讨在正常范围内轻度升高的丙氨酸转氨酶(ALT)水平与2型糖尿病患者夜间血糖之间的关系。方法将185例ALT在正常范围内的2型糖尿病患者分为低ALT组(ALT0.05)。Pearson相关性分析显示,血清ALT与夜间平均血糖(r=0.206,P=0.005)、夜间... 详细信息

目的探讨在正常范围内轻度升高的丙氨酸转氨酶(ALT)水平与2型糖尿病患者夜间血糖之间的关系。方法将185例ALT在正常范围内的2型糖尿病患者分为低ALT组(ALT0.05)。Pearson相关性分析显示,血清ALT与夜间平均血糖(r=0.206,P=0.005)、夜间最低血糖(r=0.215,P=0.003)和胰岛素抵抗指数(r=0.200,P=0.006)均相关。多元线性回归分析显示,ALT是夜间血糖的独立影响因素(标准化β=0.041,P=0.003)。结论正常范围内轻度升高的血清ALT水平与糖尿病患者夜间血糖升高有关,但与日间血糖无关。

关键词：糖尿病,2型丙氨酸转氨酶动态血糖监测

Connotation analysis, source-reservoir assemblage types and development potential of unconventional hydrocarbons in China

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Petroleum Research 2016年第2期1卷 135-148页

作者： Jianzhong Li Min Zhen xiaoming Chen Denghua Li Shaoyong Wang tao Song PetroChina Research Institute of Petroleum Exploration and Development Beijing 100083China

Through analysis of the connotation of unconventional hydrocarbons,the source-reservoir assemblages of unconventional hydrocarbons are divided into three types,i.e.,source-reservoir neighboring type,source-reservoir integration type,and source-reservoir separation *** source-reservoir neighboring type resources mainly include tight oil and tight gas with the following formation conditions and characteristics:(1)efficient near-source accumulation close to high-quality source rocks with the pressure difference between source and reservoir as the dynamic force;(2)large-area tight reservoirs with certain porosity,dominated by micro-nanopores of inorganic origin;(3)distributed in depressions and slopes with large hydrocarbon-bearing area and great resource quantity,low abundance and local development of“sweet spots”;(4)weak *** source-reservoir integration type resources mainly include coalbed methane,shale gas,shale oil and oil *** formation conditions and characteristics are:(1)high abundance of organic matter,different types of organic matter and thermal evolution grades leading to formation of different resources types;(2)organic-rich sections are distributed continuously in the reservoiring space which is dominated by nanometer organic pores,interparticle pores and microfissures;(3)hydrocarbons mainly occur in free or adsorbed state,varying much with resource type;(4)the resources are self-conserved,and enriched in depressions and slopes,the conservation conditions are controlled by tectonics,temperature and pressure;(5)the production per well can be enhanced by volume fracturing,which increases the permeability of the *** source-reservoir separation type resources are more special,mainly including(1)transformed conventional resources such as oil sand and heavy oil;(2)unsteady resources formed under special conditions of temperature and pressure,among which natural gas hydrate is a *** three types of resources in Chin

关键词： Unconventional hydrocarbons connotation source-reservoir assemblage source-reservoir neighboring type source-reservoir integration type source-reservoir separation type development potential