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Triple signal amplification electrochemical sensing platform for Hg^(2+) in water without direct modification of the working electrode

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Frontiers of Environmental Science & Engineering 2024年第7期18卷 157-167页

作者： Liuyin Hu Jiahua Cui tao lu Yalin Wang Jinping Jia School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240China International Copper Association Ltd.Shanghai 200020China

An ultrasensitive electrochemical biosensor to detect trace Hg^(2+)in environmental samples was developed utilizing nanogold-decorated magnetic reduced graphene oxide(MrGO-AuNPs),exonuclease III-assisted target cycle(Exo Ⅲ-ATC)and hybridization chain reaction(HCR)synergistic triple signal *** MrGO-AuNPs is a superior carrier for capture DNA(cDNA)and acts as magnetic media for automatic separation and *** innovative utilization of the magnetism and improved sensing efficiency obviates the need for direct modification and repeated polishing of the working ***,the three DNA hairpins(cDNA,methylene blue(MB)labeled HP1 and HP2)further contribute to biosensor specificity and *** cDNA captures Hgt,it activates Exo Ⅲ-ATC due to the formation of a sticky end in the DNA stem via thymine-Hig-thymidine(T-Hg^(2+)-T),this leads to the hydrolysis of self-folded DNA by Exo Ⅲ-ATC to form"key"DNA(kDNA).The kDNA subsequently initiates HCR,resulting in massive super-sandwich structures(kDNA-[HP1/HP2])carrying signaling molecules on MrGO-AuNPs,and this overall structure serves as a signal probe(SP).Leveraging magnetic adsorption,the SP was automatically adsorbed onto the magneto-glass carbon electrode(MGCE),generating an amplified *** biosensor's detection limit(LOD)was 3.14 pmol/L,far below the limit of 10 nmol/L for mercury in drinking water set by the US *** biosensor also showed excellent selectivity when challenged by interfering ions,and the results of its application in actual samples indicate that it has good potential for practical applications in environmental monitoring.

关键词： Electrochemical sensing Hg^(2+) Amplification Graphene Hybridization chain reaction Exonuclease Ⅲ

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Material flow analysis and global warming potential assessment of an industrial insect-based bioconversion plant using housefly larvae

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Journal of Environmental Sciences 2024年第5期139卷 483-495页

作者： tao lu Fan Lü Nanlin Liao Honghui Chai Hua Zhang Pinjing He Institute of Waste Treatment and Reclamation Tongji UniversityShanghai 200092China State Key Laboratory of Pollution Control and Source Reuse Tongji UniversityShanghai 200092China Zhejiang Qihe Ecological Technology Co. Ltd.Hangzhou 310052China

The significant increase in the demand for biomass waste treatment after garbage classification has led to housefly larvae treatment becoming an attractive treatment *** can provide a source of protein while treating biomass waste,which means that nutrients can be returned to the natural food ***,the performance of this technology in terms of its environmental impacts is still unclear,particularly with regards to global warming potential(GWP).This study used a life cycle assessment(LCA)approach to assess a housefly larvae treatment plant with a treatment capacity of 50 tons of biomass waste per *** LCA results showed that the 95% confidence intervals for the GWP in summer and winter were determined to be 24.46-32.81 kg CO_(2) equivalent(CO_(2)-eq)/ton biomass waste and5.37-10.08 kg CO_(2)-eq/ton biomass waste,*** greater GWP value in summer is due to the longer ventilation time and higher ventilation intensity in summer,which consumes more *** main GWP contributions are from(1)electricity needs(accounting for 78.6% of emissions in summer and 70.2%in winter)and(2)product substitution by mature housefly larvae and compost(both summer and winter accounting for 96.8% of carbon reduction).

关键词： Biomass waste Musca domestica Housefly larvae Life cycle assessment Global warming potential

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Immediate implantation of ultrafine fiber slow-release system based on cell electrospinning to induce osteogenesis of mesenchymal stem cells

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Regenerative Biomaterials 2024年第2期3卷 76-87页

作者： tao lu Long Yang Zhuoyang Li Yin Liu Shun'en Xu Chuan Ye Department of Orthopaedics The Affliated Hospital of Guizhou Medical UniversityGuiyang 550004China Department of Orthopaedics The First People's Hospital of GuiyangGuiyang 550004China Center for Tissue Engineering and Stem Cell Research Guizhou Medical UniversityGuiyang 550004China Department of Otolaryngology Head and Neck Surgery Xinqiao HospitalArmy Medical University(Third Military Medical University)Chongqing 400037China Department of Dental implant Stomatological Hospital of GuiyangGuiyang 550000China

This study presents the development and evaluation of a poly(3-hydroxybutyrate-co-4-hydroxybutyrate)(P34HB)ultrafine fiber slow-release system for in vivo osteogenic induction of human umbilical cord mesenchymal stem cells(HUCMSCs).Utilizing dual-nozzle and cell electrospinning techniques,the system encapsulates L-ascorbic acid-2-phosphate magnesium(AsP),βB-glycerophosphate sodium and dexamethasone(DEX)within the'fbers,ensuring sustained osteogenic *** scaffold's morphology,characterization,hydrophilicity,mechanical properties and cellular behavior were *** subcutaneous implantation in rabbits was con-ducted to observe its ectopic osteogenic induction *** fabricated P34HB ultrafine fiber slow-release *** ization *** distribution of HUCMSCs and inducing components within the scaffold,with no chemical reactions affecting the active *** vitro tests showcased a prolonged release of DEX and ASP,while biocompatibility assays highlighted the scaffold's suitability for cellular *** Red,type I collagen,and osteopontin(OPN)staining verifed the scaffold's potent osteogenic induction effect on ***,immediate implantation into New Zealand White rabbits led to significant new bone formation within 8 *** findings underscore the system's potential for immediate in vivo implantation without prior in vitro induction,marking a promising advancement in bone tissue engineering.

关键词： bone tissue engineering cell electrospinning in vivo induction immediate implantation biomaterials

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Gas sensor array based on carbon-based thin-film transistor for selective detection of indoor harmful gases

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Rare Metals 2024年第9期43卷 4401-4411页

作者： Can Liu Yu Sun Jia-Yi Guo Xiu-Lei Li lu tao Jin-Yong Hu Jue-Xian Cao Ping-Hua Tang Yong Zhang School of Electrical and Information Engineering Hunan Institute of EngineeringXiangtan 411104China Physics and Optoelectronics&Hunan Institute of Advanced Sensing and Information Technology Xiangtan UniversityXiangtan 411105China

The identification of indoor harmful gases is imperative due to their significant threats to human health and *** achieve accurate identification,an effective strategy of constructing a sensor array combined with the pattern recognition algorithm is ***-based thin-film transistors are selected as the sensor array unit,with semiconductor carbon nanotubes(CNTs)within the TFT channels modified with different metals(Au,Cu and Ti)for selective responses to NH_(3),H_(2)S and HCHO,*** accurate gas species identification,an identification mode that combines linear discriminant analysis algorithms and logistic regression classifier is *** test results demonstrate that by preprocessing the sensor array’s sensing data with the LDA algorithm and subsequently employing the LR classifier for identification,a 100%recognition rate can be achieved for three target gases(NH3,H2S and HCHO).This work provides significant guidance for future applications of chip-level gas sensors in the realms of the Internet of Things and Artificial Intelligence.

关键词： Carbon-based thin-film transistors Gas sensor arrays Semiconductor carbon nanotubes Multi-gas detection Linear discriminant analysis

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Modeling the dynamic impacts of maritime network blockage on global supply chains

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The Innovation 2024年第4期5卷 103-109,102页

作者： Shen Qu Yunlei She Qi Zhou Jasper Verschuur lu-tao Zhao Huan Liu Ming Xu Yi-Ming Wei Center for Energy and Environmental Policy Research Beijing Institute of TechnologyBeijing 100081China School of Management Beijing Institute of TechnologyBeijing 100081China Beijing Key Lab of Energy Economics and Environmental Management Beijing 100081China Oxford Programme for Sustainable Infrastructure Systems(OPSIS) Environmental Change InstituteUniversity of OxfordOX12JD OxfordUK State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex Beijing 100084China School of Environment Tsinghua UniversityBeijing 100084China

Recent phenomena such as pandemics,geopolitical tensions,and climate change-induced extreme weather events have caused transportation network interruptions,revealing vulnerabilities in the global supply chain.A salient example is the March 2021 Suez Canal blockage,which delayed 432 vessels carrying cargo valued at$92.7 billion,triggering widespread supply chain *** ability to model the spatiotemporal ramifications of such incidents remains *** fill this gap,we develop an agent-based complex network model integrated with frequently updated maritime *** Suez Canal blockage is taken as a case *** results indicate that the effects of such blockages go beyond the directly affected countries and *** Suez Canal blockage led to global losses of about$136.9($127.5–$147.3)billion,with India suffering 75%of these *** losses show a nonlinear relationship with the duration of blockage and exhibit intricate trends post *** proposed model can be applied to diverse blockage scenarios,potentially acting as an earlyalert system for the ensuing supply chain ***,high-resolution daily data post blockage offer valuable insights that can help nations and industries enhance their resilience against similar future events.

关键词： chains valued extreme

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Image Enhancement via Associated Perturbation Removal and Texture Reconstruction Learning

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IEEE/CAA Journal of Automatica Sinica 2024年第11期11卷 2253-2269页

作者： Kui Jiang Ruoxi Wang Yi Xiao Junjun Jiang Xin Xu tao lu IEEE the School of Computer Science and Technology Harbin Institute of Technology Zhengzhou Research Institute Harbin Institute of Technology the School of Artificial Intelligence Jianghan University the School of Geodesy and Geomatics Wuhan University the Hubei Province Key Laboratory of Intelligent Information Processing and Real-Time Industrial System Wuhan University of Science and Technology the School of Computer Science and Engineering and also with the Hubei Province Key Laboratory of Intelligent Robot Wuhan Institute of Technology

Degradation under challenging conditions such as rain, haze, and low light not only diminishes content visibility, but also results in additional degradation side effects, including detail occlusion and color distortion. However, current technologies have barely explored the correlation between perturbation removal and background restoration, consequently struggling to generate high-naturalness content in challenging scenarios. In this paper, we rethink the image enhancement task from the perspective of joint optimization: Perturbation removal and texture reconstruction. To this end, we advise an efficient yet effective image enhancement model, termed the perturbation-guided texture reconstruction network(PerTeRNet). It contains two subnetworks designed for the perturbation elimination and texture reconstruction tasks, respectively. To facilitate texture recovery,we develop a novel perturbation-guided texture enhancement module(PerTEM) to connect these two tasks, where informative background features are extracted from the input with the guidance of predicted perturbation priors. To alleviate the learning burden and computational cost, we suggest performing perturbation removal in a sub-space and exploiting super-resolution to infer high-frequency background details. Our PerTeRNet has demonstrated significant superiority over typical methods in both quantitative and qualitative measures, as evidenced by extensive experimental results on popular image enhancement and joint detection tasks. The source code is available at https://***/kuijiang94/PerTeRNet.

关键词： Association learning attention mechanism image enhancement perturbation modeling

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A naturally derived small molecule compound suppresses tumor growth and metastasis in mice by relieving p53-dependent repression of CDK2/Rb signaling and the Snail-driven EMT

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Chinese Journal of Natural Medicines 2024年第2期22卷 112-126页

作者： REN Boxue LI Yang DI Lei CHENG Ranran LIU Lijuan LI Hongmei LI Yi TANG Zhangrui YAN Yongming lu tao FU Rong CHENG Yongxian WU Zhaoqiu State Key Laboratory of Natural Medicines Department of PharmacologySchool of PharmacyChina Pharmaceutical UniversityNanjing 211198China Institute for Inheritance-Based Innovation of Chinese Medicine School of Pharmaceutical SciencesShenzhen University Health Science CenterShenzhen 518060China

The tumor suppressor protein p53 is central to cancer biology,with its pathway reactivation emerging as a promising therapeutic strategy in *** study introduced LZ22,a novel compound that selectively inhibits the growth,migration,and metastasis of tumor cells expressing wild-type p53,demonstrating ineffectiveness in cells devoid of p53 or those expressing mutant ***22’s mechanism of action involves a high-affinity interaction with the histidine-96 pocket of the MDM2 *** interaction disrupted the MDM2-p53 binding,consequently stabilizing p53 by shielding it from proteasomal ***22 impeded cell cycle progression and diminished cell proliferation by reinstating the p53-dependent suppression of the CDK2/Rb signaling ***,LZ22 alleviated the p53-dependent repression of Snail transcription factor expression and its consequent EMT,effectively reducing tumor cell migration and distal ***,LZ22 administration in tumor-bearing mice did not manifest notable side *** findings position LZ22 as a structurally unique reactivator of p53,offering therapeutic promise for the management of human cancers with wild-type TP53.

关键词： LZ22 Wild-type p53 p53 Reactivator Snail-driven EMT Tumor growth and metastasis

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日常话语中致歉套语「ごめん」的叠用现象研究

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日语学习与研究 2024年第1期 57-68页

作者：白雪卢涛黑龙江大学东语学院广岛大学大学院人文社会科学研究科

致歉话语研究一直是日语学界关注的重点之一。文章基于互动视角,以『日本語日常会話コーパス』中的日常话语为语料,从叠用形式、次数、话轮位置、互动功能、语用效果五方面考察了致歉套语「ごめん」的叠用情况。研究表明致歉套语「ごめ...

致歉话语研究一直是日语学界关注的重点之一。文章基于互动视角,以『日本語日常会話コーパス』中的日常话语为语料,从叠用形式、次数、话轮位置、互动功能、语用效果五方面考察了致歉套语「ごめん」的叠用情况。研究表明致歉套语「ごめん」主要表现为同语叠用,叠用次数变化阈值较大,且话轮位置灵活,不仅可以出现在话轮首、中、尾部,也可以独占话轮,当该套语位于话轮不同位置时互动功能也会随之改变,同时「ごめん」叠用也具有扩展意向、强化信息与人际关系、渲染心理状态的独特语用效果。

关键词：日语致歉ごめん叠用形式互动功能语用效果

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现代性、景观消费和身体战争——关于身体问题的历时性考察

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内蒙古社会科学 2024年第3期45卷 134-140页

作者：陆涛江西师范大学新闻与传播学院江西南昌330022

从工业时代起,机器剥离了人的工具性,身体重新在文化中找到自己的主体性。自此,身体就一直处于祛魅—赋魅的循环之中。工业文化使得身体失去了自己的劳动地位,只有被附会成商品,才能在拜物教中寻找到自己的一席之地。从美颜相机到短视...

从工业时代起,机器剥离了人的工具性,身体重新在文化中找到自己的主体性。自此,身体就一直处于祛魅—赋魅的循环之中。工业文化使得身体失去了自己的劳动地位,只有被附会成商品,才能在拜物教中寻找到自己的一席之地。从美颜相机到短视频直播,21世纪发生了一场以身体消费为主角的狂欢。身体作为视觉文化的重要组成部分,在信息科学时代沦为赋魅的他者,被随意地修改和扭曲。身体或者说身体的符号价值成为橱窗中的商品,而贩卖这种价值的电影院与信息网络则成为21世纪的“巴黎拱廊”。二者都表现出现代性对物的膜拜和对主体的消解。身体时刻处于看与被看、指涉与被指涉的关系中,数字资本掠夺走了身体在虚体中产生的价值,一场对身体价值进行争夺的战争在后人类时代开始了。

关键词：现代性景观消费身体战争

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两种智慧:作为复合创新文化的设计智造

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装饰 2024年第7期 54-65页

作者：卢涛熊娜中国美术学院

随着技术环境与文化语境的转变,当代设计文化在数据驱动下不可避免地介入到技术变革与文化转向的互动中。本文通过对不同时期设计文化内涵与价值的探讨,思考并审视了由科技智造与人文智性两种智慧交融共生所形成的设计智造复合创新文化...

随着技术环境与文化语境的转变,当代设计文化在数据驱动下不可避免地介入到技术变革与文化转向的互动中。本文通过对不同时期设计文化内涵与价值的探讨,思考并审视了由科技智造与人文智性两种智慧交融共生所形成的设计智造复合创新文化,并结合DIA大奖多组得奖作品案例,从“去中心化的组织形态、面对危机的可持续系统形态、人机共融的后人类形态”这三个视角,提出复合创新的文化本质在于以“技术理智与人文心智”的双向汇通来保持设计的生命力,文化内核在于以“人的发现与生活世界经验的重建”来激发多元丰富、流动交织而又不断进化的创造力。对于助推设计文化的创新研究具有一定的意义。

关键词：设计文化 DIA大奖设计智造社会技术体系复合创新

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