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Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

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Chinese Science Bulletin 2008年第19期53卷 2973-2980页

作者： YI ChuanDeng tang shuzhu ZHOU Yong LIANG GuoHua GONG ZhiYun GU MingHong Jiangsu Province Key Laboraty of Crop Genetics and Physiology/Key Laboraty of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China

Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chro- mosome pairing was identified and most of the chromosomes were univalent, which resulted in com- plete male sterility in the hybrid.

关键词：漂白亚麻纤维卷胚芽种间杂交技术

Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311

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The Crop Journal 2023年第3期11卷 887-894页

作者： Zuopeng Xu Yuanyue Du Xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao Qiaoquan Liu shuzhu tang Honggen Zhang Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural CollegeYangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina School of Life Sciences Nantong UniversityNantong 226019JiangsuChina

The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon *** used widely in three-line indica *** identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type *** we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type *** developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting *** analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were ***,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene *** results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines.

关键词： Rice WA-type CMS Restorer of fertility(Rf)gene Gene mapping

Wx^lv、the Ancestral Allele of Rice Waxy Gene

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Molecular Plant 2019年第8期12卷 1157-1166页

作者： Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi shuzhu tang Minghong Gu Qiaoquan Liu Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding College of AgricultureYangzhou UniversityYangzhou 225009China Co-lnnovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou UniversityYangzhou 225009China

In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking *** effects of several Wx alleles on amylose content and the taste of cooked rice have been ***,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain *** we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose *** demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional *** data indicate that the Wx^lv allele played an important role in artificial selection and *** findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice.

关键词： Oryza sativa Waxy eating and cooking quality allelic variation artificial selection

A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency

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Journal of Integrative Plant Biology 2021年第5期63卷 889-901页

作者： Changquan Zhang Yong Yang Shengjie Chen Xueju Liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei Fan shuzhu tang Minghong Gu Qiaoquan Liu Key Laboratory of Crop Genomics and Molecular Breeding of Jiangsu Province State Key Laboratory of Hybrid RiceYangzhou UniversityYangzhou 225009China Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Co‐Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province Yangzhou UniversityYangzhou 225009China State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongHong KongChina

In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs.

关键词： allelic variation amylose content(AC) eating and cooking quality(ECQ) grain appearance Oryza sativa L. Waxy

Development of gene-tagged molecular markers for starch synthesis-related genes in rice

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Chinese Science Bulletin 2010年第33期55卷 3768-3777页

作者： TIAN ZhiXi YAN ChangJie QIAN Qian YAN Song XIE HuiLan WANG Fang XU JieFen LIU GuiFu WANG YongHong LIU QiaoQuan tang shuzhu LI JiaYang GU MingHong Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Key Laboratory of Plant Functional Genomics Ministry of Education of China Jiangsu Key Laboratory of Crop Genetics and Physiology Agricultural College of Yangzhou University Yangzhou 225009 China China National Rice Research Institute Hangzhou 310006 China

Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target *** marker-aided selection(MAS),combined with conventional breeding approaches,enables us to pre-cisely identify the individual genotypes that are associated with different grain quality features,which can dramatically improve the breeding ***,to date,the number of molecular markers used in MAS for grain quality improvement is still somewhat *** this study,based on our previous study that rice grain quality is strongly associated with starch synthesis in the endosperm,we developed 51 gene-tagged molecular markers according to sequence variations in 18 starch synthesis-related genes from 16 typical rice *** markers can discriminate the different alleles among rice *** novel markers will provide effective tools in improving grain quality via the breeding new elite rice varieties.

关键词：分子标记辅助选择基因型鉴定水稻育种淀粉合成粮食质量基因序列变异有效工具育种方法

Preliminary study on the genes related to the fertility of indica/japonica hybrid rice

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Chinese Rice Research Newsletter 2000年第4期8卷 5-6页

作者： YAN Changjie LIANG Guohua tang shuzhu LU Jufei GU Minghong Dept of Agronomy Agri College Yangzhou Univ Yangzhou 225009 China

Studying the sterility of indica/japonica hybrid was a prerequisite for the utilization of heterosis in high yield rice breeding. In 1986, Ikehashi et al found that wide compatibility varieties, which possessed S5-n allele, couldovercome the sterility of indica/japonica hybrids, and thesterilitv was mainly caused by allelic interaction at S-5 lo-cus. Further studies indicated that the sterility could notbe completely overcome just by S5-n. There were must beother loci responsible for the sterility of indica/ japonicahybrids.

关键词： Preliminary study on the genes related to the fertility of indica/japonica hybrid rice

Fine mapping and cloning of MT1,a novel allele of D10

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Progress in Natural Science:Materials International 2009年第12期19卷 1683-1689页

作者： Yong Zhou,Jinyan Zhu,Zhengyi Li,Fei Gu,Honggen Zhang,shuzhu tang, Minghong Gu,Guohua Liang~* Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology,Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University,Yangzhou 225009,China Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China

Rice tillering is an important determinant for grain *** investigate the mechanism of tillering,we characterized a multiple tillering mutant(mt1) identified from the japonica variety,Zhonghua 11,treated with *** mutant exhibits advanced tillering development and dwarfed compared with wild-type *** analysis and fine gene mapping indicated that the mt1 mutant was controlled by a recessive gene,residing on a 29-kb window on AP003376 of chromosome *** putative gene in this region,encoding a carotenoid cleavage dioxygenase 8(CCD8),was allelic to *** mt1 mutant phenotype was complemented by introduction of wild-type MT1,and knockdown of MT1 in wild-type rice mimicked the mutant ***-time PCR analysis indicated that the MT1 gene is expressed highly in stems and at a low level in axillary buds,panicles,leaves,and *** addition,MT1 expression is clearly under feedback regulation.

关键词： Rice Tillering Map-based cloning OsCCD8 Feedback regulation

基于改进NSGA2的临近空间通信网络优化设计

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计算机工程与应用 2018年第14期54卷 203-210页

作者：唐树祝游鹏闫大伟雍少为国防科学技术大学电子科学与工程学院长沙410005

面向特定区域部署的临近空间通信网络需要兼顾考虑资源分配、覆盖率及载荷功率等多个因素。考虑到现有方法只采用单目标优化,以及非支配排序遗传算法(NSGA2)在求解实际问题时的缺陷等问题,提出一种基于改进NSGA2的临近空间通信网络多目... 详细信息

面向特定区域部署的临近空间通信网络需要兼顾考虑资源分配、覆盖率及载荷功率等多个因素。考虑到现有方法只采用单目标优化,以及非支配排序遗传算法(NSGA2)在求解实际问题时的缺陷等问题,提出一种基于改进NSGA2的临近空间通信网络多目标优化设计方法。分析下行链路性能,结合目标区域的需求分布,以最大化匹配度和覆盖率及最小化功耗代价为目标建立多目标优化模型。将动态反向学习机制和差分局部变异算子引入NSGA2,测试证明改进的NSGA2在解收敛性和分布性上表现更好。仿真验证了设计方法的有效性,为网络的实际部署提供了参考。

关键词：临近空间通信网络多目标优化非支配排序遗传算法

Molecular marker for screening rice cultivars with intermediate amylose content in indica

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Chinese Rice Research Newsletter 2002年第Z1期 7-9页

作者： CAI Xiuling, LIU Qiaoquan, tang shuzhu, Gu Minghong, and WANG Zongyang, Shanghai Inst of Plant Physiology, the Chinese Academy of Sciences, Shanghai 200032 College of Bioscience and Biotechnology, Yangzhou Univ, Yangzhou 225009, China

The waxy (Wx) gene of rice encodes the granule-bound starch synthase (GBSS), which was required in the synthesis of amylose in endosperm. The amylose content (AC) of rice endosperm played an important role in grain yield, palatability, and processing quality. Our previous researches showed that endosperm AC and GBSS contents were correlated with the ability of excising intron 1 from the leader sequence of the Wx transcript. Cultivars with high endosperm

关键词： GBSS PCR Molecular marker for screening rice cultivars with intermediate amylose content in indica AC