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RNAi knockdown of PIK3CA preferentially inhibits invasion of mutant PIK3CA cells

World Journal of Gastroenterology 2011年第32期17卷 3700-3708页

作者： Xin-Ke Zhou Sheng-song tang Gao Yi Min Hou Jin-Hui Chen Bo Yang Ji-Fang Liu Zhi-Min He Department of Pharmacology University of South China Hengyang 421001 Hunan Province China Institute of Cancer Research Affiliated Tumor Hospital of Guangzhou Medical College Guangzhou 510095 Guangdong Province China Laboratory of Applied Marine Biology Southem China Sea Institute of Oceanology Chinese Academy of Science Guangzhou 510300 Guangdong Province China

AIM: To explore the effects of siRNA silencing of PIK3CA on proliferation, migration and invasion of gastric cancer cells and to investigate the underlying mechanisms. METHODS: The mutation of PIK3CA in exons 9 and 20 of gastric cancer cell lines HGC-27, SGC-7901, BGC-823, MGC-803 and MKN-45 was screened by polymerase chain reaction (PCR) followed by sequencing. BGC-823 cells harboring no mutations in either of the exons, and HGC-27 cells containing PIK3CA mutations were employed in the current study. siRNA targeting PIK3CA was chemically synthesized and was transfect- ed into these two cell lines in vitro . mRNA and protein expression of PIK3CA were detected by real-time PCR and Western blotting, respectively. We also measured phosphorylation of a serine/threonine protein kinase (Akt) using Western blotting. The proliferation, migration and invasion of these cells were examined separately by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet-razolium bromide (MTT), wound healing and Transwell chambers assay. RESULTS: The siRNA directed against PIK3CA effectively led to inhibition of both endogenous mRNA and protein expression of PIK3CA, and thus significantly down-regulated phosphorylation of Akt (P < 0.05). Furthermore, simultaneous silencing of PIK3CA resulted in an obvious reduction in tumor cell proliferation activity, migration and invasion potential (P < 0.01). Intriguing, mutant HGC-27 cells exhibited stronger invasion ability than that shown by wild-type BGC-823 cells. Knockdown of PIK3CA in mutant HGC-27 cells contributed to a reduction in cell invasion to a greater extent than in non-mutant BGC-823 cells. CONCLUSION: siRNA mediated targeting of PIK3CA may specifically knockdown the expression of PIK3CA in gastric cancer cells, providing a potential implication for therapy of gastric cancer.

关键词： Gastric cancer Metastasis PIK3CA PI3K/ Akt pathway RNAi

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Circulating biomarker-and magnetic resonance-based nomogram predicting long-term outcomes in dilated cardiomyopathy

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Chinese Medical Journal 2024年第1期137卷 73-81页

作者： Yupeng Liu Wenyao Wang Jingjing song Jiancheng Wang Yi Fu Yida tang Department of Cardiology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhou 510080China Guangdong Cardiovascular Institute Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Department of Cardiology Institute of Vascular MedicinePeking University Third HospitalBeijing 100191China Department of Cardiology State Key Laboratory of Cardiovascular DiseaseFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems State Key Laboratory of Natural and Biomimetic DrugsSchool of Pharmaceutical SciencesPeking UniversityBeijing 100083China Department of Physiology and Pathophysiology School of Basic Medical SciencesPeking University Health Science CenterBeijing 100083China Key Laboratory of Molecular Cardiovascular Science Ministry of EducationBeijing 100191China

Background:Dilated cardiomyopathy(DCM)has a high mortality rate and is the most common indication for heart *** study sought to develop a multiparametric nomogram to assess individualized all-cause mortality or heart transplantation(ACM/HTx)risk in DCM ***:The present study is a retrospective cohort *** demographic,clinical,blood test,and cardiac magnetic resonance imaging(CMRI)data of DCM patients in the tertiary center(Fuwai Hospital)were *** primary endpoint was ACM/*** least absolute shrinkage and selection operator(LASSO)Cox regression model was applied for variable *** Cox regression was used to develop a *** concordance index(C-index),area under the receiver operating characteristic curve(AUC),calibration curve,and decision curve analysis(DCA)were used to evaluate the performance of the ***:A total of 218 patients were included in the present *** were randomly divided into a training cohort and a validation *** nomogram was established based on eight variables,including mid-wall late gadolinium enhancement,systolic blood pressure,diastolic blood pressure,left ventricular ejection fraction,left ventricular end-diastolic diameter,left ventricular end-diastolic volume index,free triiodothyronine,and N-terminal pro-B type natriuretic *** AUCs regarding 1-year,3-year,and 5-year ACM/HTx events were 0.859,0.831,and 0.840 in the training cohort and 0.770,0.789,and 0.819 in the validation cohort,*** calibration curve and DCA showed good accuracy and clinical utility of the ***:We established and validated a circulating biomarker-and CMRI-based nomogram that could provide a personalized prediction of ACM/HTx for DCM patients,which might help risk stratification and decision-making in clinical practice.

关键词： Cardiomyopathy,dilated Heart transplantation Mortality Nomograms Least absolute shrinkage and selection operator Decision curve analysis

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Health implications of engineered nanoparticles in infants and children

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World Journal of Pediatrics 2015年第3期11卷 197-206页

作者： song tang Mao Wang Kaylyn E Germ Hua-Mao Du Wen-Jie Sun Wei-Min Gao Gregory D Mayer The Institute of Environmental and Human Health Texas Tech UniversityLubbockTexas 79416USA School of Environment and Sustainability University of SaskatchewanSaskatoonSaskatchewan S7N 5B3Canada Department of Preventive Medicine School of Public HealthSun Yat-sen UniversityGuangzhou 510080China College of Biotechnology Southwest UniversityBeibeiChongqing 400715China School of Food Science Guangdong Pharmaceutical UniversityZhongshan 528458China Department of Global Health and Environmental Sciences School of Public Health and Tropical MedicineTulane UniversityNew OrleansLouisiana 70112USA

Background:The nanotechnology boom and the ability to manufacture novel nanomaterials have led to increased production and use of engineered nanoparticles(ENPs).However,the increased use of various ENPs inevitably results in their release in or the contamination of the environment,which poses significant threats to human *** recent years,extraordinary economic and societal benefits of nanoproducts as well as their potential risks have been observed and widely *** estimate whether ENPs are safe from the onset of their manufacturing to their disposal,evaluation of the toxicological effects of ENPs on human exposure,especially on more sensitive and vulnerable sectors of the population(infants and children)is *** sources:Papers were obtained from PubMed,Web of Science,and Google *** search words included:"nanoparticles","infants","children","exposure","toxicity",and all relevant ***:A brief overview was conducted to 1)characterize potential exposure routes of ENPs for infants and children;2)describe the vulnerability and particular needs of infants and children about ENPs exposure;3)investigate the current knowledge about the potential health hazards of ENPs;and 4)provide suggestions for future research and regulations in ENP ***:As the manufacturing and use of ENPs become more widespread,directed and focused studies are necessary to measure actual exposure levels and to determine adverse health consequences in infants and children.

关键词： developmental effects human exposure maternal-fetal transmission nanopediatrics nanotoxicity

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基于 XYZ/ E的混成系统(英文)

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软件学报 2000年第1期11卷 1-7页

作者：阎安唐稚松中国科学院软件研究所计算机科学开放研究实验室北京100080

混成系统是由计算机和物理设备组成的嵌入式实时计算系统 .它允许在交互式实时系统中引入连续变化的单元 .XYZ/ E是基于 Manna- Pnueli的线性时序逻辑的程序设计语言 .它将程序的动态语义与静态语义统一在同一框架中 ,支持从抽象的程序... 详细信息

混成系统是由计算机和物理设备组成的嵌入式实时计算系统 .它允许在交互式实时系统中引入连续变化的单元 .XYZ/ E是基于 Manna- Pnueli的线性时序逻辑的程序设计语言 .它将程序的动态语义与静态语义统一在同一框架中 ,支持从抽象的程序规范到可执行代码的逐步求精的全过程 .该文使用 XYZ/ E语言描述和验证混成系统 .首先介绍了计算模型 ,然后介绍了 XYZ语言对混成系统的形式化描述 ,最后介绍了混成系统的验证 .与同类工作相比 ,XYZ/ E支持状态转换 ,从而可以方便地描述复杂的控制算法 .

关键词：混成系统时序逻辑语言程序规范 XYZ/E语言

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数字电流控制单电感双输出开关变换器研究

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制造业自动化 2024年第8期46卷 39-48页

作者：唐嵩峰周述晗贺明智陈茂林林润泽四川大学电气工程学院四川成都610065

为解决单电感双输出(Single Inductor Dual Output,SIDO)开关变换器中电流型模拟控制技术存在的稳定性问题,提出了一种瞬态性能优异、稳定性强的数字电流控制技术。以工作于电感电流连续导电模式(Continuous Conduction Mode,CCM)的SIDO... 详细信息

为解决单电感双输出(Single Inductor Dual Output,SIDO)开关变换器中电流型模拟控制技术存在的稳定性问题,提出了一种瞬态性能优异、稳定性强的数字电流控制技术。以工作于电感电流连续导电模式(Continuous Conduction Mode,CCM)的SIDO Buck变换器为研究对象,根据不同的脉宽调制模式,分别分析了数字电流控制技术前缘调制和后缘调制的工作原理,推导出两种调制模式下的控制算法。其次,建立采样数据模型,运用分岔图分析了输入、输出电压对数字电流控制SIDO Buck变换器稳定性的影响。研究结果表明:数字电流控制SIDO Buck变换器的负载瞬态响应速度快、输出支路交叉影响小;不同于电流型模拟控制技术,数字电流控制技术的两种调制模式中,前缘调制的稳定性不受输入、输出电压的影响,而后缘调制的稳定性受输入、输出电压的影响。最后通过实验结果验证了理论分析的正确性。

关键词：数字电流控制连续导电模式单电感双输出脉宽调制模式稳定性

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在 XYZ/ E中实现混成实时系统——蒸气锅炉控制问题的解决(英文)

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软件学报 2000年第6期11卷 711-719页

作者：闫安唐稚松中国科学院软件所计算机科学开放研究实验室北京100080

XYZ/E is a temporal logic system as well as a programming language. The temporal logic language XYZ/E can represent both high level and low level specifications in the same framework, so that the specification and implementation of software systems are very convenient. A specification and an implementation of the Steam Boiler Control Specification Problem in XYZ/E is presented in this paper. A visual user interface is also implemented.

关键词：时序逻辑语言混成实时系统描述 XYZ/E

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A narrative review on the role of temperature and humidity in COVID-19:Transmission,persistence,and epidemiological evidence

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Eco-Environment & Health 2022年第2期1卷 73-85页

作者： Yuan Wei Zhaomin Dong Wenhong Fan Kaiqiang Xu song tang Ying Wang Fengchang Wu State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China School of Space and Environment Beihang UniversityBeijing 102206China Beijing Advanced Innovation Center for Big Data-Based Precision Medicine Beihang UniversityBeijing 100083China China CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 211166China

Since December 2019,the 2019 coronavirus disease(COVID-19)outbreak has become a global *** the role of environmental conditions is important in impeding the spread of *** that airborne spread and contact transmission are considered the main pathways for the spread of COVID-19,this narrative review first summarized the role of temperature and humidity in the airborne trajectory of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Meanwhile,we reviewed the persistence of the virus in aerosols and on inert surfaces and summarized how the persistence of SARS-CoV-2 is affected by temperature and *** also examined the existing epidemiological evidence and addressed the limitations of these epidemiological *** uncertainty remains,more evidence may support the idea that high temperature is slightly and negatively associated with COVID-19 growth,while the conclusion for humidity is still ***,the spread of COVID-19 appears to have been controlled primarily by government interventions rather than environmental factors.

关键词： SARS-CoV-2 Temperature Humidity Aerosol Transmission

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Energy consumption mechanism simulation and experimental study of reciprocating vibration for Chinese wolfberry picking

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International Journal of Agricultural and Biological Engineering 2024年第4期17卷 146-155页

作者： song Mei Dunbing tang Zhigang Shi Zhiyu song Cheng Shen College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China Nanjing Institute of Agricultural Mechanization Ministry of Agriculture and Rural AffairsNanjing 210014China Institute of Chinese Wolfberry Science Ningxia Academy of Agriculture and Forestry SciencesYinchuan 750002China

In order to find out the matching principle of excitation force and energy consumption of reciprocating vibrating Chinese wolfberry picking device,the energy consumption mechanism of reciprocating vibrating Chinese wolfberry picking device is *** to the structural characteristics and operating principle of the picking device,the no-load and load movement and force characteristics of the crank bearing and vibrating component are analyzed,and the theoretical model of energy consumption of the reciprocating vibration picking device is jointly constructed,and the simulation analysis is carried *** results show that the vibrating component and load mass have a significant influence on torque,the load air resistance phase has a significant effect on torque,and the load air resistance and friction coefficient have no significant influence on ***,by building an AC servo motor torque detection system and a torque sensing detection system,verification experiments are carried out,the maximum torque of the preset system is 1.3 N∙m,the rated power is 400 W,the motor frequency is 20 Hz,the amplitude is 15 mm,and the total mass of the vibrating component is 0.143 *** results show that,the no-load operation,the change trend of detected torque is consistent with simulation,the torque model is verified to be *** maximum torque of simulation and detection are 0.52 N∙m and 0.57 N∙m respectively,and the error between test and simulation is 9.6%.For load operation,the maximum torque of five groups of branch loads of 20 g,60 g,100 g,140 g,180 g and 220 g are detected to be 0.73 N∙m,0.74 N∙m,0.75 N∙m,0.82 N∙m and 0.83 N∙m,respectively,and the relationship model between load and torque is obtained by *** research results can provide a theoretical basis which can configure a suitable motor in the reciprocating vibration Chinese wolfberry picking device with a certain load limit.

关键词： Chinese wolfberry branches vibration component load torque power

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Mapping Changes of Whole Brain Blood Flow in Rats with Myocardial Ischemia/Reperfusion Injury Assessed by Positron Emission Tomography

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Current Medical Science 2019年第4期39卷 653-657页

作者： Xu-chu PAN Zhi-xiao LI Duo-zhi WU Shun-yuan LI Hong-bing XIANG Yong-tang song Medical Association of Hubei Province Wuhan 430071China Department of Anesthesiology and Pain Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Anesthesiology Hainan General HospitalHaikou 570311China Department of Anesthesiology the First Affiliated Quanzhou Hospital of Fujian Medical UniversityQuanzhou 362000China

l8F-labeled fluorodeoxyglucose positron emission tomography (l8F-FDG PET) is the most sensitive tool for studying brain metabolism in *** investigated the image patterns of 18F-FDG PET during reperfusion injury and correlated changes of whole brain blood flow utilizing a rat myocardial ischemia/reperfiision injury (MIRI) *** results assessed by echocardiography indicated resultant cardiac dysfunction after ischemia-reperfusion in the rat *** was found that the average standardized uptake value (SUVaverage) of the whole brain was significantly decreased in model rats,and the glucose uptake of different brain regions including accumbens core/shell (Acb),left caudate putamen (LCPu),hippocampus (HIP),left hypothalamus (LHYP),olfactory (OLF),superior colliculus (SC),right midbrain (RMID),ventral tegmental area (VTA),inferior colliculus (IC) and left thalamus whole (LTHA) was significantly decreased in MIRI rats whereas no significant difference was found in the SUVaverage of amygdala (AMY),right Cpu,RHYP,right HYP,left MID,right THA,pons and medulla oblongata (MO).These l8F-FDG PET data provide a reliable identification method for brain metabolic changes in rats with MIRI.

关键词： cardiac ischemia-reperfusion injury brain metabolism 18-fluorodeoxyglucose positron emission tomography

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Green carbonization of waste coffee grounds into porous C/Fe hybrids for broadband and high-efficiency microwave absorption

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Journal of Materials Science & Technology 2024年第3期170卷 1-10页

作者： Xin Wen Cui Li Hui Liu Guohua Fan Yanan tang Chuncheng Hao Lili Ma Pingan song Institute of Advanced Electrical Materials College of Materials Science and EngineeringQingdao University of Science and TechnologyQingdao 266042China Center for Future Materials University of Southern QueenslandSpringfieldQLD 4300Australia School of Agriculture and Environmental Science University of Southern QueenslandSpringfieldQLD 4300Australia

Recently great effort s have been focused on designing high-performance microwave absorbers using sustainable biomass resources,but there remains a lack of green and efficient fabrication ***,inspired by natural porous character of biomass waste,we demonstrated a green one-step route to convert waste coffee grounds into porous C/Fe hybrids,and further explored their potential applications for broadband and high-efficiency microwave *** this design,the WCG-20-750(incorporated 20 wt%Fe(C_(5)H_(7)O_(2))_(3)catalyst and carbonized at 750℃)exhibited porous microstructure with the highest char yield of 55.45 wt%.Furthermore,the as-prepared C/Fe hybrids from WCG-20-750 displayed excellent microwave absorption ***,the minimum reflection loss(RL_(min))reached to-52.86 dB and the widest effective absorption bandwidth(EAB)was 6.40 GHz at the thickness of 3.0 *** work provides an economically viable and environmentally friendly strategy to convert biomass wastes into value-added microwave absorbers,ultimately making contributions to the upcycling of renewable biomass resources and the fostering of sustainable environment.

关键词： Waste recycling Microwave absorption Green carbonization Porous carbon Biomass

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