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Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第1期68卷 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu rong Gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University Department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词： neurotransmitters panic disorder magnetic solid phase extraction direct analysis of mass spectrometry in real time

Liposomes as versatile agents for the management of traumatic and nontraumatic central nervous system disorders:drug stability,targeting efficiency,and safety

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Neural Regeneration Research 2025年第7期20卷 1883-1899页

作者： Mingyu Zhang Chunyu Xiang Renrui Niu Xiaodong He Wenqi Luo Wanguo Liu rui Gu Department of Orthopedic Surgery China-Japan Union Hospital of Jilin UniversityChangchunJilin ProvinceChina

Various nanoparticle-based drug delivery systems for the treatment of neurological disorders have been widely ***,their inability to cross the blood–brain barrier hampers the clinical translation of these therapeutic *** are nanoparticles composed of lipid bilayers,which can effectively encapsulate drugs and improve drug delivery across the blood–brain barrier and into brain tissue through their targeting and ***,they can potentially treat traumatic and nontraumatic central nervous system *** this review,we outlined the common properties and preparation methods of liposomes,including thin-film hydration,reverse-phase evaporation,solvent injection techniques,detergent removal methods,and microfluidics ***,we comprehensively discussed the current applications of liposomes in central nervous system diseases,such as Alzheimer's disease,Parkinson's disease,Huntington's disease,amyotrophic lateral sclerosis,traumatic brain injury,spinal cord injury,and brain *** studies related to liposomes are still in the laboratory stage and have not yet entered clinical ***,their application as drug delivery systems in clinical practice faces challenges such as drug stability,targeting efficiency,and ***,we proposed development strategies related to liposomes to further promote their development in neurological disease research.

关键词： Alzheimer's disease amyotrophic lateral sclerosis brain tumors central nervous system Huntington's disease liposome drug delivery neurological disorders Parkinson's disease spinal cord injury traumatic brain injury

基于中药复方适度调节原理与广义免疫组平衡监测的中医证候研究与中药复方药效评价新思路、新方法

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中国中药杂志 2018年第16期43卷 3229-3234页

作者：张翼冠谭蕊蓉任思冲马欣严志祥华桦鄢良春戴瑛赵军宁四川省中医药转化医学中心四川省中医药科学院转化药理研究所国家中医药管理局中药质量生物评价重点研究室四川省道地药材系统开发工程技术研究中心中药品质评价与创新中药研究四川省重点实验室

中医证候客观化与中药复方药效学评价标准一直是中医中药基础研究难以突破的关键环节。现代免疫学认为，机体是一个平衡体，免疫失衡是百病之源。中医诊治疾病的思维方式也是“平衡”，认为生病是因为人体“偏性”，即“阴阳、表里、寒... 详细信息

中医证候客观化与中药复方药效学评价标准一直是中医中药基础研究难以突破的关键环节。现代免疫学认为，机体是一个平衡体，免疫失衡是百病之源。中医诊治疾病的思维方式也是“平衡”，认为生病是因为人体“偏性”，即“阴阳、表里、寒热、虚实”等失衡所致。整合医学催生了广义免疫组学，免疫功能（免疫平衡）系统评价已是整合医学诊疗模式的重要依据，而平衡也是中医整体观核心思想。由此，可以预见，广义免疫组学整体或局部组织平衡原理为中医整体观与现代整合医学桥接理论基础之一，是中医症候客观化研究与中药复方药效评价的重要依据之一。由此，笔者根据动态、整体精准医学模式背景，根据新病因观，提出运用信息学、统计学方法构建基于广义免疫组学整体与局部组织平衡原理的中医证候客观化与中药复方评价体系。该体系将会深入揭示中医整体观理论科学内涵，为中药复方药效评价提供新思路新方法，亦可作为亚健康人群免疫力筛查，为中医分子治未病学科发展提供理论支持等。随着临床样本量与数据量不断积累，该评价体系将更精准、更智能。

关键词：广义免疫组新病因观中西医整体观桥接依据中药复方适度调节原理中医证候中药复方

Combination of fruquintinib with venetoclax for the treatment of colorectal cancer

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Oncology Research 2025年第1期33卷 225-234页

作者： WEI ZHANG WEICHENG WANG rui WANG XIAO HAN LIJUN ZHU WENJIE GUO YANHONG GU Department of Oncology The First Affiliated Hospital of Nanjing Medical UniversityNanjing210029China State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing210023China Department of Oncology The Affiliated Taizhou People’s Hospital of Nanjing Medical UniversityTaizhou225300China

Background:As a novel blocker of vascular endothelial growth factor receptor(VEGFR),fruquintinib has been approved for treating colorectal cancer(CRC).However,its dosage and therapeutic efficacy are limited by its widespread adverse ***,recognized as the initial inhibitor of B-cell lymphoma protein 2(BCL2),has shown potential in boosting the effectiveness of immunotherapy against *** study investigated the efficacy and mechanisms of fruquintinib combined with venetoclax in treating *** and Materials:We developed a colon cancer mouse model with the CT26 colon cell line to demonstrate fruquintinib and venetoclax’s efficacy against *** we employed various techniques to evaluate different aspects of the experimental *** was used to detect cell proliferation and angiogenesis in tumor *** blot analysis was utilized to examine the occurrence of cell apoptosis,and flow cytometry to quantitate immune cells within the tumor ***,immunofluorescence was employed to measure cytokine ***:The strongest inhibition on tumor growth was achieved by the combination of fruquintinib with venetoclax,as opposed to individual drug *** was found to amplify the impact of fruquintinib,leading to decreased cancer cell proliferation,increased cancer cell apoptosis,lowered angiogenesis,better vascular structure normalization,and improved immune cell ***:Our findings indicate that the addition of venetoclax enhances the impact of fruquintinib on vascular normalization and modulation of the tumor immune *** study presents the justification for utilizing the fruquintinib and venetoclax combination in treating *** holds promise in being assimilated into anticancer medications for CRC.

关键词： Colorectal cancer(CRC) B-cell lymphoma protein 2(BCL2) Venetoclax Vascular endothelial growth factor receptor(VEGFR) Fruquintinib Anti-angiogenesis Immunotherapy

Robust interface and excellent as-built mechanical properties of Ti–6Al–4V fabricated through laser-aided additive manufacturing with powder and wire

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 154-168页

作者： Fei Weng Guijun Bi Youxiang Chew Shang Sui Chaolin tan Zhenglin Du Jinlong Su Fern Lan Ng Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of EducationShandong Engineering&Technology Research Center for Superhard MaterialSchool of Materials Science and EngineeringShandong UniversityJinan 250061China Institute of Intelligent Manufacturing Guangdong Academy of SciencesGuangzhou 510095China Singapore Institute of Manufacturing Technology 5 Cleantech Loop636732Singapore

The feasibility of manufacturing Ti-6Al-4V samples through a combination of laser-aided additive manufacturing with powder(LAAM_(p))and wire(LAAM_(w))was explored.A process study was first conducted to successfully circumvent defects in Ti-6Al-4V deposits for LAAM_(p) and LAAM_(w),*** the optimized process parameters,robust interfaces were achieved between powder/wire deposits and the forged substrate,as well as between powder and wire *** characterization results revealed the epitaxial prior β grains in the deposited Ti-6Al-4V,wherein the powder deposit was dominated by a finerα′microstructure and the wire deposit was characterized by lamellar α *** mechanisms of microstructure formation and correlation with mechanical behavior were analyzed and *** mechanical properties of the interfacial samples can meet the requirements of the relevant Aerospace Material Specifications(AMS 6932)even without post heat *** fracture occurred within the interfacial area,further suggesting the robust *** findings of this study highlighted the feasibility of combining LAAM_(p) and LAAM_(w) in the direct manufacturing of Ti-6Al-4V parts in accordance with the required dimensional resolution and deposition rate,together with sound strength and ductility balance in the as-built condition.

关键词： laser-aided additive manufacturing powder deposition wire deposition interfacial characteristic mechanical behavior

伊尹汤液之谜——中药复方非典型药理效应规律发现与评价策略

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中国中药杂志 2022年第16期47卷 4261-4268页

作者：戴瑛张翼冠曾瑾华桦赵军宁李莉鄢良春尹竹君王剑波谭鹏谭蕊蓉曾安琪全云云魏平四川省中医药科学院四川省中医药转化医学中心国家中医药管理局中药质量生物评价重点研究室中医药转化医学四川省重点实验室四川省道地药材形成原理与品质评价工程研究中心四川省道地药材系统开发工程技术研究中心四川成都610041

伊尹制汤液而始有方剂,由此发展出的现代中药复方及其制剂以其在慢性多因素性疾病的良好治疗效果而备受关注,代表了未来新药发现的一个重要方向。中药复方具有特殊的或者被忽视的独特作用原理,这与单一成分作用的“神弹理论”有较大差别... 详细信息

伊尹制汤液而始有方剂,由此发展出的现代中药复方及其制剂以其在慢性多因素性疾病的良好治疗效果而备受关注,代表了未来新药发现的一个重要方向。中药复方具有特殊的或者被忽视的独特作用原理,这与单一成分作用的“神弹理论”有较大差别,也与现代多靶点药物的内涵不尽相同。“伊尹汤液理论(Yiyin decoction theory)”是基于现代科学技术方法描述和阐释这种具有超前意识的个性化组合用药的特殊药理效应机制,既考虑到疾病/证候的非稳定性,也注意到疾病在受到药理干扰时表现出来的顽固性和自适应性,以及疾病的多因素病因之间相互关联性,作者把其科学内涵概括为“复方配伍,多重效应,适度调治”。复方配伍涉及组成药物君臣佐使药之间复杂性相互作用。多重效应涉及多种常规的以及非常规的作用机制、非线性反馈相互作用的综合药理效应。适度调治则反映其最终可从整体上精准地实现对疾病生物网络的多点嵌套调控。要破解“伊尹汤液”之谜,并非仅仅简单研究已知活性物质(成分)及其在混合物中独立的靶点作用,更需要重新发现和认识中药复方的“暗物质”“暗效应”,即常规药理效应规律之外的、被忽视的非典型药理效应规律以及在生物体内发挥精准调控功能的多点嵌合机制。伊尹汤液理论聚焦中药复方的整体药理效应规律以体现其综合的临床价值,对于发展符合中医药理论的中药复方新药评价与转化应用新模式具有重要意义。

关键词：伊尹汤液中药复方神弹理论多靶点药物非典型药理效应适度调节原理

Federated local causal structure learning

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Science China(Information Sciences) 2025年

作者： Kui YU Chen rong Hao WANG Fuyuan CAO Jiye LIANG School of Computer and Information Hefei University of Technology School of Computer and Information Technology Shanxi University

Local causal structure learning (LCS) efficiently identifies a set of direct neighbors of a specified variable from observational data. Additionally, it distinguishes direct causes and direct effects of this variable without learning the entire causal structure. While many LCS algorithms have been proposed, they do not consider the data privacy-preserving problem,which has attracted extensive attention from academia and industry. To address this issue, we propose a federated local causal structure learning (FedLCS) algorithm to learn local causal structures in privacy-preserving data in a federated ***, FedLCS introduces a layer-wise federated local skeleton learning algorithm to construct the local skeleton. Based on this skeleton, it introduces a federated local skeleton orientation algorithm and an extension-and-backtracking orientation algorithm to orient the edges. Finally, FedLCS uses a federated local extension-and-backtracking orientation algorithm to orient the remaining edges. Extensive experiments on benchmark, synthetic, and real datasets demonstrate that FedLCS can learn the local causal structure of a given variable in a federated setting.

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Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

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Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao Lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing Fang Liangming Zhang Bin Liu Limin rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

Li,Na,and Cs-based metal-organic frameworks for room temperature phosphorescence applications

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Science China Chemistry 2025年

作者： Jiaqi Ma rui Feng Yanyuan Jia Mingshi Zhang Shuo Guo College of Chemistry and Chemical Engineering Inner Mongolia University School of Materials Science and Engineering Smart Sensing Interdisciplinary Science Center Nankai University & TKL of Metal and Molecule Based Material Chemistry Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University Department of Chemistry and Biochemistry University of California San Diego

The conventional design of metal-organic frameworks (MOFs) with room-temperature phosphorescence (RTP) mostly relies on using heavy metals,as the heavy atom effect can effectively increase the efficiency of intersystem crossing (ISC) to get a better phosphorescence *** the first time,we reported the highly efficient RTP of a lithium-based MOF IMU-101 with a lifetime (τ) of 299 ms and a quantum yield (QY) of 4.91%.In addition,MOFs IMU-102 and IMU-103,with sodium and cesium as metal nodes,were successfully *** phosphorescence properties of this series of alkali metal MOFs were systematically studied and *** crystallography and computational calculation show that the mechanism for achieving RTP for this material lies in the ultrashort coordination bond of Li,which enables the reduction of non-radiative ***-101,as a cheap and environmentally friendly room-temperature phosphorescent material,has been further developed into composite polymer inks and bulk *** makes them promising for applications in anti-counterfeiting and information encryption.

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