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MYB34, MYB51, and MYB 122 Distinctly Regulate Indolic Glucosinolate Biosynthesis in Arabidopsis thaliana

Molecular Plant 2014年第5期7卷 814-828页

作者： Henning Frerigmann tamara gigolashvili Botanical Institute and Cluster of Excellence on Plant Sciences (CEPLAS) University of Cologne Cologne Biocenter D-50674 Cologne Germany

The MYB34, MYB51, and MYB122 transcription factors are known to regulate indolic glucosinolate （IG） biosynthesis in Arabidopsis thaliana. To determine the distinct regulatory potential of MYB34, MYB51, and MYB122, the accumulation of IGs in different parts of plants and upon treatment with plant hormones were analyzed in A. thaliana seedlings. It was shown that MYB34, MYB51, and MYB122 act together to control the biosynthesis of 13M in shoots and roots, with MYB34 controlling biosynthesis of IGs mainly in the roots, MYB51 regulating biosynthesis in shoots, and MYB122 having an accessory role in the biosynthesis of IGs. Analysis of glucosinolate levels in seedlings of myb34, myb51, myb122, myb34 myb51 double, and myb34 myb51 myb122 triple knockout mutants grown in the presence of abscisic acid （ABA）, salicylic acid （SA）, jasmonate （JA）, or ethylene （ET） revealed that：（1） MYB51 is the central regulator of IG synthesis upon SA and ET signaling, （2） MYB34 is the key regulator upon ABA and JA signaling, and （3） MYB122 plays only a minor role in JA/ET-induced glucosinolate biosynthesis. The myb34 myb51 myb122 triple mutant is devoid of IGs, indicating that these three MYB factors are indispensable for IG production under standard growth conditions.

关键词： indolic glucosinolates regulation R2R3 MYB the triple myb34 myb51 myb122 mutant.

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Regulation of Pathogen-Triggered Tryptophan Metabolism in Arabidopsis thaliana by MYB Transcription Factors and Indole Glucosinolate Conversion Products

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Molecular Plant 2016年第5期9卷 682-695页

作者： Henning Frerigmann Mariola Pislewska-Bednarek Andrea Sanchez-Vallet Antonio Molina Erich Glawischnig tamara gigolashvili Pawel Bednarek Botanical Institute and Cluster of Excellence on Plant Sciences(CEPLAS) University of Cologne BioCenter Zulpicher Straβe 47b 50674 Cologne Germany Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego12/14 61-704 Pozna(n) Poland Centro de Biotecnologiay Genomica de Plantas Unlversidad Politecnica de Madrid Campus Montegancedo Pozuelo de Alarcon 28223 Madrid Spain Lehrstuhl for Genetik Technische Universitat Munchen Emil-Ramann-Str. 8 85354 Freising Germany ETH Zurich Department of Environmental Systems Science GHN H41 Universitatsstrasse 16 8092 Zurich Switzerland

MYB34, MYB51, and MYB122 transcription factors are known as decisive regulators of indolic glucosinolate （IG） biosynthesis with a strong impact on expression of genes encoding CYP79B2 and CYP79B3 enzymes that redundantly convert tryptophan to indole-3-acetaldoxime （IAOx）. This intermediate represents a branching point for IG biosynthesis, and pathways leading to camalexin and indole-carboxylic acids （ICA）. Here we investigate how these MYBs affect the pathogen-triggered Trp metabolism. Our experiments indicated that these three MYBs affect not only IG production but also constitutive biosynthesis of other IAOx- derived metabolites. Strikingly, the PENETRATION 2 （PEN2）-dependent IG-metabolism products, which are absent in myb34/51/122 and pen2 mutants, were indispensable for full flg22-mediated induction of other IAOx-dedved compounds. However, germ induction and accumulation of ICAs and camalexin upon path- ogen infection was not compromised in myb34/51/122 plants, despite strongly reduced IG levels. Hence, in comparison with cyp79B2/B3, which lacks all IAOx-derived metabolites, we found myb34/51/122 an ideal tool to analyze IG contribution to resistance against the necrotrophic fungal pathogen Plectosphaerella cucumerina. The susceptibility of myb34/51/122 was similar to that of pen2, but much lower than susceptibility of cyp79B2/B3, indicating that MYB34/51/122 contribute to resistance toward P. cucumerina exclu- sively through IG biosynthesis, and that PEN2 is the main leaf myrosinase activating IGs in response to microbial pathogens.

关键词： indolic glucosinate P/ectosphaere//a cucumerina MAMP-triggered immunity Trp metabolism indole-3-carboxylic acids camalexin

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