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Anomeric Proton and Carbon (H1-C1) NMR Chemical Shifts of Antigenic Mannans Obtained from Pathogenic Yeast Candida tropicalis

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Advances in Microbiology 2021年第5期11卷 296-301页

作者： takuya kuraoka Takayoshi Yamada Yuki Takatsutsumi Yukiko Ogawa Hidemitsu Kobayashi Divisions of Microbiology Department of Pharmaceutical Science Nagasaki International University Nagasaki Japan Divisions of Infection Control and Prevention Department of Pharmaceutical Science Nagasaki International University Nagasaki Japan

On two dimensional maps of 1H-13C correlation spectroscopy (H-C COSY) analysis for the mannan of Candida tropicalis, nine cross peaks of anomeric proton and carbon were useful for the purpose of obtaining information on the chemical structure of this molecule. Namely, the mannans was comb-like structure constructed with the linear α-1,6-linked polymannnosyl backbone and several oligomannnosyl side chains composed of α-1,2-, α-1,3-, and β-1,2-linkages. Therefore, in the structural investigation of comb-like mannan, two-dimensional H-C COSY analysis is as useful as two-dimensional nuclear Hartmann-Hahn (HOHAHA) analysis.

关键词： Candida tropicalis Cell Wall Mannan Comb-Like Structure 1H-13C Correlation Spectroscopy Anomeric Carbon Chemical Shift α-1 3-Linked Mannose

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Determination of α-1,3-Linked Mannose Residue in the Cell Wall Mannan of Candida tropicalisNBRC 1400 Strain

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Advances in Microbiology 2020年第1期10卷 14-26页

作者： takuya kuraoka Takayoshi Yamada Akito Ishiyama Hiroko Oyamada Yukiko Ogawa Hidemitsu Kobayashi Divisions of Microbiology Nagasaki International University Huis Ten Bosch Sasebo Nagasaki Japan Sanko Medical Tomato Pharmacy Co. Ltd. Shimogumigo Kawatanacho Nagasaki Japan Sasebo City General Hospital Hirasecho Sasebo Nagasaki Japan Divisions of Infection Control and Prevention Department of Pharmaceutical Science Nagasaki International University Huis Ten Bosch Sasebo Nagasaki Japan

To investigate the chemical structure of cell wall mannan obtained from pathogenic yeast, Candida tropicalis NBRC 1400 (former antigenic standard strain, IFO 1400). As a result of two-dimensional NMR analysis, it was shown that the mannan of this strain is composed of α-1,6-, α-1,3-, α-1,2- and β-1,2-linked mannose residues. In this research, the mannan was subjected to three degradation procedures, acid-treatment, α-mannosidase, and acetolysis under two conditions in order to determine the chemical structure of the antigenic oligomannosyl side chains in this molecule. The 1H-nuclear magnetic resonance spectra of resultant oligosaccharides, pentaose and hexaose, demonstrated the existence of the oligomannosyl side chains corresponding to Manα1-3Manα1-2Manα1-2Manα1-2Man and Manα1-3Manα1-2Manα1-2Manα1-2Manα1-2Man, respectively, which have previously also been found in Candida albicans serotype A strain mannans. These findings indicate that C. tropicalis and C. albicans serotype A have no significant difference in the chemical structure of these cell wall mannans. Therefore, it can be interpreted that it is extremely difficult to distinguish both species by targeting the antigenic group in these mannans.

关键词： Candida tropicalis Pathogenic Yeast Cell Wall Mannan Antigenic Oligomannosyl Side Chain Acetolysis α-1 3-Linked Mannose Residue

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