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Liquid biopsy in patients with hepatocellular carcinoma: Circulating tumor cells and cell-free nucleic acids

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World Journal of Gastroenterology 2017年第31期23卷 5650-5668页

作者： wataru okajima shuhei komatsu daisuke ichikawa mahito miyamae takuma ohashi taisuke imamura jun kiuchi keiji nishibeppu tomohiro arita hirotaka konishi atsushi shiozaki ryo morimura hisashi ikoma kazuma okamoto eigo otsuji division of digestive surgery department of surgery kyoto prefectural university of medicine

Hepatocellular carcinoma(HCC), with its high incidence and mortality rate, is one of the most common malignant tumors. Despite recent development of a diagnostic and treatment method, the prognosis of HCC remains poor. Therefore, to provide optimal treatment for each patient with HCC, more precise and effective biomarkers are urgently needed which could facilitate a more detailed individualized decision-making during HCC treatment, including the following; risk assessment, early cancer detection, prediction of treatment or prognostic outcome. In the blood of cancer patients, accumulating evidence about circulating tumor cells and cell-free nucleic acids has suggested their potent clinical utilities as novel biomarker. This concept, so-called "liquid biopsy" is widely known as an alternative approach to cancer tissue biopsy. This method might facilitate a more sensitive diagnosis and better decision-making by obtaining genetic and epigenetic aberrations that are closely associated with cancer initiation and progression. In this article, we review recent developments based on the available literature on both circulating tumor cells and cell-free nucleic acids in cancer patients, especially focusing on Hepatocellular carcinoma.

关键词： Hepatocellular carcinoma Biomarker Liquid biopsy Circulating tumor cells Cell-free nucleic acids

Anoctamin 5 regulates the cell cycle and affects prognosis in gastric cancer

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World Journal of Gastroenterology 2022年第32期28卷 4649-4667页

作者： Tomoyuki Fukami Atsushi Shiozaki Toshiyuki Kosuga Michihiro Kudou Hiroki Shimizu takuma ohashi Tomohiro Arita Hirotaka Konishi Shuhei Komatsu Takeshi Kubota Hitoshi Fujiwara Kazuma Okamoto Mitsuo Kishimoto Yukiko Morinaga Eiichi Konishi Eigo Otsuji Division of Digestive Surgery Department of SurgeryKyoto Prefectural University of MedicineKyoto 602-8566Japan Department of Pathology Kyoto City HospitalKyoto 604-8845Japan Department of Pathology Kyoto Prefectural University of MedicineKyoto 602-8566Japan

BACKGROUND Anoctamin 5(ANO5)/transmembrane protein 16E belongs to the ANO/transmembrane protein 16 anion channel *** comprise a family of plasma membrane proteins that mediate ion transport and phospholipid scrambling and regulate other membrane proteins in numerous cell *** studies have elucidated the roles and mechanisms of ANO5 activation in various cancer ***,it remains unclear whether ANO5 acts as a plasma membrane chloride channel,and its expression and functions in gastric cancer(GC)have not been *** To examine the role of ANO5 in the regulation of tumor progression and clinicopathological significance of its expression in *** Knockdown experiments using ANO5 small interfering RNA were conducted in human GC cell lines,and changes in cell proliferation,cell cycle progression,apoptosis,and cellular movement were *** gene expression profiles of GC cells were investigated following ANO5 silencing by microarray *** staining of ANO5 was performed on 195 primary tumor samples obtained from patients with GC who underwent curative gastrectomy between 2011 and 2013 at our *** Reverse transcription-quantitative polymerase chain reaction(PCR)and western blotting demonstrated high ANO5 mRNA and protein expression,respectively,in NUGC4 and MKN45 *** these cells,ANO5 silencing inhibited cell proliferation and induced *** addition,the knockdown of ANO5 inhibited G1-S phase progression,invasion,and *** results of the microarray analysis revealed changes in the expression levels of several cyclin-associated genes,such as CDKN1A,CDK2/4/6,CCNE2,and E2F1,in ANO5-depleted NUGC4 *** expression of these genes was verified using reverse transcription-quantitative *** staining revealed that high ANO5 expression levels were associated with a poor *** analysis identified high ANO5 expression as an independent prognostic fa

关键词： Anoctamin 5 Gastric cancer Cell cycle G1/S checkpoint Cell proliferation

Retinal ganglion cell protection by hop-flower extract as a novel neuroprotective strategy for glaucoma

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Neural Regeneration Research 2022年第6期17卷 1267-1268页

作者： Tomoko Hasegawa Hanako ohashi Ikeda Department of Ophthalmology and Visual Sciences Kyoto University Graduate School of MedicineKyotoJapan Research Fellow of Japan Society for the Promotion of Science TokyoJapan

In glaucoma,a leading cause of blindness,retinal ganglion cells are progressively *** pressure reduction is the only established treatment for glaucoma(Collaborative Normal-Tension Glaucoma Study Group,1998;Vass et al.,2007).However,in some cases,visual field loss progresses despite sufficiently reduced intraocular pressure(Collaborative Normal-Tension Glaucoma Study Group,1998;Killer and Pircher,2018).While intraocular pressure and age are known risk factors for glaucoma(Ernest et al.,2013),the underlying mechanisms of glaucoma progression are not fully understood.

关键词： glaucoma al. protective

Adenosine triphosphate maintenance by branched chain amino acids as a novel neuroprotective strategy for retinal neurodegenerative diseases

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Neural Regeneration Research 2019年第1期14卷 82-84页

作者： Tomoko Hasegawa Hanako ohashi Ikeda Department of Ophthalmology and Visual Sciences Kyoto University Graduate School of Medicine Department of Experimental Therapeutics Institute for Advancement of Clinical and Translational Science Kyoto University Hospital

Retinal neuronal cell death is caused in many incurable eye diseases such as retinitis pigmentosa(RP)and glaucoma,which are leading causes of adult *** RP,progressive loss of photoreceptor cells leads to visual *** established treatments are available to date for this condition,although potential treatments,including regenerative medicine,gene therapy,and neurotropic factor therapy are being *** glaucoma。

关键词：眼睛疾病视网膜氨基酸基因治疗腺苷分叉维护光敏电阻器

Cryopreserved fat: our clinical experience and applications

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Plastic and Aesthetic Research 2020年第5期7卷 47-56页

作者： Masanori ohashi The Clinic Tokyo Minato-kuTokyo 106-0031Japan

Aim:Cryopreservation of fat is an effective method for repeat fat grafting,but there are few reports about the clinical use of cryopreserved *** aim of this study was to determine the effectiveness and safety of cryopreserved fat for clinical ***:Between Aug 2015 and Dec 2018,we investigated 590 patients who underwent fat harvesting at our *** harvested fat was cryopreserved at a temperature of-196°C at a cell processing center and injections were performed in our ***:Of the 590 patients studied,216(312 cases)have undergone fat injections so *** augmentations using harvested fat,such as facial and breast augmentations,were performed on 180 *** 84 patients,harvested fat was utilized only for revitalization/fertilization purposes,such as to improve skin *** were no severe complications in any ***,volume maintenance was rarely *** rejuvenation effects were comparable to that in cases using fresh ***:The clinical use of cryopreserved fat is thought to be safe and effective.

关键词： Cryopreserved fat frozen fat fat grafting skin rejuvenation

Modulation of valosin-containing protein by Kyoto University Substances(KUS) as a novel therapeutic strategy for ischemic neuronal diseases

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Neural Regeneration Research 2017年第8期12卷 1252-1255页

作者： Masayuki Hata Hanako ohashi Ikeda Department of Ophthalmology and Visual Sciences Kyoto University Graduate School of Medicine Neuroprotective Treatment Project for Ocular Diseases Institute for Advancement of Clinical and Translational Science Kyoto University Hospital

Retinal ischemia causes several vision-threatening diseases, including diabetic retinopathy, retinal artery occlusion, and retinal vein occlusion. Intracellular adenosine triphosphate（ATP） depletion and subsequent induced endoplasmic reticulum（ER） stress are proposed to be the underlying mechanisms of ischemic retinal cell death. Recently, we found that a naphthalene derivative can inhibit ATPase activity of valosin-containing protein, universally expressed within various types of cells, including retinal neural cells, with strong cytoprotective activity. Based on the chemical structure, we developed novel valosin-containing protein modulators, Kyoto University Substances（KUSs）, that not only inhibit intracellular ATP depletion, but also ameliorate ER stress. Suppressing ER stress by KUSs is associated with neural cell survival in animal models of several neurodegenerative diseases, such as glaucoma and retinal degeneration. Given that a major pathology of ischemic retinal diseases, other than intracellular ATP depletion, is ER stress-induced cell death, KUSs may provide a novel strategy for cell protection in ischemic conditions. Hence, we investigated the efficacy of KUS121 in a rat model of retinal ischemic injury. Intravitreal injections of KUS121, which is clinically preferable route of drug administration in retinal diseases, significantly suppressed inner retinal thinning and retinal cell death, and maintained visual functions. Valosin-containing protein modulation by KUS is a promising novel therapeutic strategy for ischemic retinal diseases.

关键词： adenosine triphosphatase C/EBP homologous protein central retinal artery occlusion endoplasmicreticulum stress neuroprotective therapy retinal ganglion cell

Gorenstein Q-homology projective planes

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Science China Mathematics 2015年第3期58卷 501-512页

作者： HWANG DongSeon KEUM JongHae ohashi Hisanori Department of Mathematics Ajou University School of Mathematics Korea Institute For Advanced Study Department of Mathematics Faculty of Science and Technology Tokyo University of Science

We present the complete list of all singularity types on Gorenstein Q-homology projective planes,i.e.,normal projective surfaces of second Betti number one with at worst rational double *** list consists of 58 possibl... 详细信息

关键词： Q-homology projective plane Enriques surface rational double point

Local oxynitriding of AZ31 magnesium alloy by atmospheric-pressure plasma treatment at room temperature

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Journal of Magnesium and Alloys 2022年第7期10卷 1878-1886页

作者： Kenta Nakazawa Tatsuki ohashi Shotaro Saiki Shoichi Kikuchi Department of Mechanical Engineering Faculty of EngineeringShizuoka University3-5-1JohokuNaka-kuHamamatsu432-8561Japan Graduate School of Integrated Science and Technology Shizuoka University3-5-1 JohokuNaka-kuHamamatsu432-8561Japan

A surface-hardening treatment for AZ31 magnesium alloy using an atmospheric-pressure plasma jet(APPJ)at room temperature was *** is a potential engineering material because it is lightweight;however,magnesium alloys are difficult to heat-treat because of their low flaming *** alloy specimens were irradiated with a localized atmospheric-pressure plasma jet generated by dielectric-barrier discharge for 180 s in *** APPJ excited oxygen and nitrogen molecules in the ambient air,resulting in the formation of an oxynitrided layer;oxygen and nitrogen diffusion layer,on the surface of the magnesium *** hardness and elemental distribution for the treated surface were *** top surface of the APPJ-treated magnesium alloy achieved a maximum hardness of 108 HV,which was~1.7 times greater than that of the untreated *** analysis using an electron-probe microanalyzer revealed strong oxygen and nitrogen signals corresponding to the hardened region of the magnesium alloy,meaning that the hardness increased as a result of the formation of the oxynitrided *** proposed APPJ treatment is a promising approach for locally hardening magnesium alloys without using a heat treatment.

关键词： Magnesium alloy Oxynitriding Atmospheric-pressure plasma jet Hardness Surface modification Diffusion

Development of Bio-cultured Artificial Muscles with High Design Flexibility

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Journal of Bionic Engineering 2023年第4期20卷 1635-1645页

作者： Hirono ohashi Shunsuke Shigaki Ryo Teramae Masahiro Shimizu Koh Hosoda Department of Systems Innovation Osaka University1-2MachikaneyamaToyonakaOsaka560-0043Japan

Recent advances in bionics have made it possible to create various tissue and *** this cell culture technology,engineers have developed a robot driven by three-dimensional cultured muscle cells(bioactuator)—a muscle cell *** more applications,researchers have been developed various tissues and organs with bio3D ***,three-dimensional cultured muscle cells printed by bio3D printer have been not used for muscle cell robot *** aim of our study is to develop easy fabrication method of bioactuator having high design flexibility like as bio3D *** fabricated three-dimensional cultured muscle cells using mold and dish having pin which can contribute to shape and cell *** this study,we observed that our method maintained the shape of three-dimensional cultured muscle cells and caused cell alignment which is important for bioactuator *** named three-dimensional cultured muscle cells developed in this study“bio-cultured artificial muscle(BiCAM)”.Finally,we observed that BiCAM contracted in response to electrical *** these data,we concluded our proposed method is easy fabrication method of bioactuator having high design flexibility.

关键词： Three-dimensional cultured muscle cells Bio-cultured artificial muscle Skeletal muscle cell Tissue engineering