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Inertial mixing of acoustically levitated droplets for time-lapse protein crystallography

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Droplet 2024年第3期3卷 91-101页

作者： Soichiro Tsujino Yohei sato Shichao Jia Michal W.Kepa Sofia Trampari takashi Tomizaki Division of Biology and Chemistry Paul Scherrer InstitutVilligenSwitzerland Swiss Nanoscience Institute University of BaselBaselSwitzerland Division Scientific Computing Theory and DataPaul Scherrer InstitutVilligenSwitzerland Biozentrum University of BaselBaselSwitzerland Photon Science Division Paul Scherrer InstitutVilligenSwitzerland

Varying the chemical consistency of acoustically levitated droplets opens up an in situ study of chemical and biochemical reactions in small ***,the opti-mization of the mixing time and the minimization of the positional instability induced by solution dispensing are necessary for practical applications such as the study of the transient state of macromolecules crystallography during the ligand binding *** this purpose,we study the inertial mixing in a configuration compatible with the room-temperature crystallography using the acoustic levitation diffractometer,therein solution drops ejected at high velocity collide and coalesce with droplets dis-pensed on acoustically levitated and rotating polymer thin-film sample *** the proposed method,we are able to achieve the mixing time of∼0.1 s for sub-micro and a few microliter *** observed short mixing time is ascribed to the rapid penetration of the solution into the droplets and confirmed by a computational fluid dynamic *** demonstrated accelerated solution mixing is tested in a pilot time-lapse protein crystallography experiment using the acoustic levitation *** results indicate the detection of transient ligand binding state within 2 s after the solution dispensing,suggesting the feasibility of the proposed method for studying slow biochemical processes.

关键词： mixing droplets transient

Correlation between dose-volume parameters and rectal bleeding after 12 fractions of carbon ion radiotherapy for prostate cancer

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World Journal of Radiology 2024年第7期16卷 256-264页

作者： takashi Ono Hiraku sato Yuya Miyasaka Yasuhito Hagiwara Natsuko Yano Hiroko Akamatsu Mayumi Harada Mayumi Ichikawa Department of Radiation Oncology Faculty of MedicineYamagata UniversityYamagata 990-9585Japan Department of Heavy Particle Medical Science Yamagata University Graduate School of Medical ScienceYamagata 990-9585Japan

BACKGROUND Carbon ion radiotherapy(CIRT)is currently used to treat prostate *** bleeding is a major cause of toxicity even with ***,to date,a correlation between the dose and volume parameters of the 12 fractions of CIRT for prostate cancer and rectal bleeding has not been ***,the clinical risk factors for rectal bleeding were absent after 12 fractions of *** To identify the risk factors for rectal bleeding in 12 fractions of CIRT for prostate *** Among 259 patients who received 51.6 Gy[relative biological effectiveness(RBE)],in 12 fractions of CIRT,15 had grade 1(5.8%)and nine had grade 2 rectal bleeding(3.5%).The dose-volume parameters included the volume(cc)of the rectum irradiated with at least x Gy(RBE)(Vx)and the minimum dose in the most irradiated x cc normal rectal volume(Dx).RESULTS The mean values of D6cc,D2cc,V10 Gy(RBE),V20 Gy(RBE),V30 Gy(RBE),and V40 Gy(RBE)were significantly higher in the patients with rectal bleeding than in those *** cutoff values were D6cc=34.34 Gy(RBE),D2cc=46.46 Gy(RBE),V10 Gy(RBE)=9.85 cc,V20 Gy(RBE)=7.00 cc,V30 Gy(RBE)=6.91 cc,and V40 Gy(RBE)=4.26 *** D2cc,V10 Gy(RBE),and V20 Gy(RBE)cutoff values were significant predictors of grade 2 rectal *** The above dose-volume parameters may serve as guidelines for preventing rectal bleeding after 12 fractions of CIRT for prostate cancer.

关键词： Carbon ion radiotherapy Prostate cancer Rectal bleeding Dose volume parameters Prevention

基于转录组测序和网络药理学探究青蒿琥酯干预U87和U251脑胶质瘤细胞的药效差异机制

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药学学报 2023年第6期58卷 1475-1483页

作者：李涛毛霞张彦琼林娜 sato takashi MIZUNO Koji OKUYAMA Katsuki 黄丰云南中医药大学中药学院云南昆明650500 中国中医科学院中药研究所北京100700 东京药科大学生物化学系东京都八王子市192-0392

青蒿琥酯具有干预脑胶质瘤的潜能,但其药理作用机制尚不明确。本研究首先基于U87和U251两种人源脑胶质瘤细胞,探究青蒿琥酯对细胞活性、增殖和凋亡的影响,发现青蒿琥酯对U87细胞活性和增殖能力的抑制作用强于对U251细胞;Hoechst和TUNEL... 详细信息

青蒿琥酯具有干预脑胶质瘤的潜能,但其药理作用机制尚不明确。本研究首先基于U87和U251两种人源脑胶质瘤细胞,探究青蒿琥酯对细胞活性、增殖和凋亡的影响,发现青蒿琥酯对U87细胞活性和增殖能力的抑制作用强于对U251细胞;Hoechst和TUNEL细胞凋亡染色实验也发现,青蒿琥酯可以显著促进U87细胞的凋亡(P 利用青蒿琥酯处理前后的U87和U251细胞裂解液,通过转录组测序检测和差异数据分析,分别获得青蒿琥酯敏感性和非敏感性细胞株的差异基因集以及青蒿琥酯效应相关基因集,旨在挖掘青蒿琥酯对两类细胞敏感性不一的分子机制。通过构建上述各对比组差异基因互作网络和计算网络拓扑特征值,筛选药效相关的关键网络靶标;生物功能富集分析结果表明,上述关键网络靶标显著富集于多条与脑胶质瘤病理环节相关的信号通路,其中,与细胞凋亡相关的转录激活因子4 (ATF4)-DNA损伤诱导转录因子3 (DDIT3)-聚腺苷二磷酸核糖聚合酶1 (PARP1)信号轴的富集显著性最高。分子对接结果也表明,青蒿琥酯与ATF4和DDIT3均具有较好的结合能力。本研究初步揭示了青蒿琥酯干预U87脑胶质瘤细胞的潜能与其抑制肿瘤细胞内异常活化的ATF4-DDIT3-PARP1信号轴,进而诱导细胞凋亡有关;同时也表明了PARP1可能是U251细胞对青蒿琥酯产生耐药的重要靶标。相关结果为青蒿琥酯治疗脑胶质瘤,以及解决脑胶质瘤治疗药物耐药的现状提供了新的实验依据。

关键词：青蒿琥酯脑胶质瘤转录组测序网络药理学药理机制

基于DNA甲基化测序技术研究青蒿琥酯干预脑胶质瘤的分子机制

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中草药 2023年第6期54卷 1814-1824页

作者：闫向英毛霞李涛 Koji Mizuno Katsuki Okuyama 林娜张彦琼 takashi sato 中国中医科学院中药研究所北京100700 东京药科大学生物化学系东京192-0392

目的探究青蒿琥酯干预胶质母细胞瘤(glioblastoma,GBM)的药效及其潜在分子机制。方法采用Cell TiterBlue检测青蒿琥酯对U87和U2512种人源胶质母细胞瘤细胞活性和增殖的影响;流式细胞术检测青蒿琥酯对2种细胞周期的调节作用;采用q RT-PCR... 详细信息

目的探究青蒿琥酯干预胶质母细胞瘤(glioblastoma,GBM)的药效及其潜在分子机制。方法采用Cell TiterBlue检测青蒿琥酯对U87和U2512种人源胶质母细胞瘤细胞活性和增殖的影响;流式细胞术检测青蒿琥酯对2种细胞周期的调节作用;采用q RT-PCR和Western blotting检测青蒿琥酯对2种细胞自噬水平的影响。采用DNA甲基化联合网络药理学从表观遗传学层面研究青蒿琥酯干预GBM的靶标基因,并建立蛋白质-蛋白质相互作用网络,通过计算网络中各节点的网络拓扑特征值,筛选出青蒿琥酯干预GBM的核心靶点并进行生物功能富集分析;采用AutoDock v4.2.6和Auto Dock Vina v1.2.0软件开展分子对接虚拟计算,考察青蒿琥酯与其候选关键靶点的结合能力,并采用Western blotting验证青蒿琥酯对2种脑胶质瘤细胞中关键靶点表达的影响。结果青蒿琥酯可显著抑制U87细胞的活性和增殖(P利用给药前后的U87与U251细胞进行DNA甲基化测序与网络药理学整合分析,在获得青蒿琥酯敏感型和耐药型脑胶质瘤细胞的差异基因集,以及青蒿琥酯干预2种细胞效应基因集的基础上,发现其干预2类脑胶质瘤细胞的关键网络靶标共同参与GBM恶性进展密切相关的血小板衍生生长因子受体α(platelet derived growth factor receptorα,PDGFRA)-大鼠肉瘤基因(rat sarcoma viral oncogene homolog,RAS)-B-Raf原癌基因(B-Raf proto-oncogene,BRAF)-丝裂原活化蛋白激酶激酶(mitogenactivated protein kinase kinase,MEK)-细胞外信号调节激酶(extracellular signal regulated kinase,ERK)通路,且青蒿琥酯与其直接靶标(PDGFRA、BRAF)具有较强的亲和力。Western blotting实验证实青蒿琥酯可以抑制U87和U251细胞中关键靶点PDGFRA和BRAF蛋白的表达(P<0.05、0.01、0.001)。结论青蒿琥酯可能通过靶向调控PDGFRA-RAS-BRAF-MEKERK信号通路,从而抑制GBM细胞的生长和扩散,为青蒿琥酯治疗GBM提供了体外实验依据,也为解决临床抗肿瘤药物的耐药问题提供了新思路和新方法。

关键词：青蒿琥酯胶质母细胞瘤 DNA甲基化分析耐药 PDGFRA-RAS-BRAF-MEK-ERK信号通路

Sensorineural hearing dysfunction after discharge from critical care in adults:A retrospective observational study

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Journal of Otology 2021年第3期16卷 144-149页

作者： takashi Fujiwara Mizuki sato Shin-ichi sato Toshio Fukuoka Department of Public Health Research Kurashiki Clinical Research InstituteMiwa 1-1-1Kurashiki CityOkayama Prefecture710-8602Japan Department of Otolaryngology Head and Neck Surgery Kurashiki Central HospitalMiwa 1-1-1Kurashiki CityOkayama Prefecture710-8602Japan Department of Critical Care and Emergency Medicine Kurashiki Central HospitalMiwa 1-1-1Kurashiki CityOkayama Prefecture710-8602Japan Emergency and Critical Care Center Kurashiki Central HospitalMiwa 1-1-1Kurashiki CityOkayama Prefecture710-8602Japan

Background Patients undergoing intensive care are exposed to risk factors for hearing *** study assessed the worse changes in pure tone average(PTA)thresholds after intensive care and identified the factors associated with worse hearing *** We conducted a single-centre retrospective study,and included adult patients admitted to the intensive care unit(ICU)of Kurashiki Central Hospital between January 2014 and September 2019,who had regular pure tone audiometry performed before and after ICU *** between changes in PTA threshold and patient characteristics,were *** included ears were classified as those with worse hearing(>10 dB increase in the PTA threshold)and those without worse hearing,and the baseline characteristics were *** During the study period,125 ears of 71 patients(male:female ratio,35:36;mean age,72.5±12.3 years)met the eligibility ***,sex,and the use of furosemide were not correlated with changes in PTA *** analysis showed that ears with worse hearing were associated with a lower serum platelet count than ears without worse hearing(153±85×10^(9)/L vs.206±85×10^(9)/L,respectively;P=0.010),and the rate of planned ICU admission(elective surgery)was higher in the worse hearing group(57.1% vs.28.8%,respectively;p=0.011).Conclusions Age,sex,and the use of furosemide did not have adversely affect hearing *** serum platelet count and planned admission appear to be risk factors for worse hearing.

关键词： Hearing loss Critical care Intensive care Adverse effects Observational study

Pseudogap,Fermi arc,and Peierls-insulating phase induced by 3D-2D crossover in monolayer VSe2

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Nano Research 2019年第1期12卷 165-169页

作者： Yuki Umemoto Katsuaki Sugawara Yuki Nakata takashi Takahashi Takafumi sato Department of Physics Tohoku UniversitySendai 980-8578Japan WPI-Advanced Institute for Materials Research Tohoku UniversitySendai 980-8577Japan Center for Spintronics Research Network Tohoku UniversitySendai 980-8577Japan

One of important challenges in condensed-matter physics is to realize new quantum states of matter by manipulating the dimensionality of materials,as represented by the discovery of high-temperature superconductivity in atomic-layer pnictides and room-temperature quantum Hall effect in *** sition-metal dichalcogenides(TMDs)provide a fertile platform for exploring novel quantum phenomena accompanied by the dimensionality change,since they exhibit a variety of electronic/magnetic states owing to quantum *** we report an anomalous metal-i nsulator transition in duced by three-dimensional(3D)-two-dimensional(2D)crossover in mono layer 1T-VSe2 grown on bilayer *** observed a complete insulating state with a finite energy gap on the entire Fermi surface in monolayer 1T-VSe2 at low temperatures,in sharp contrast to metallic nature of *** surprisingly,monolayer 1T-VSe2 exhibits a pseudogap with Fermi arc at temperatures above the charge-density-wave temperature,showing a close resemblanee to high-temperature *** similarity suggests a common underlying physics between two apparently different systems,pointing to the importance of charge/spin fluctuations to create the novel electronic states,such as pseudogap and Fermi arc,in these materials.

关键词： transition-metal dichalchogenides 1T-VSe2 charge density wave electronic states pseudogap Fermi arc

离子排斥色谱用于厌氧消化处理的水中脂肪族羧酸的分析(英文)

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色谱 2012年第4期30卷 369-373页

作者： Kazuaki ITO Jun SAKAMOTO Kazuya NAGAOKA Yohichi TAKAYAMA takashi KANAHORI Hiroshi SUNAHARA Tsuneo HAYASHI Shinji sato Takeshi HIROKAWA Kazuhiko TANAKA Department of Biotechnology and Chemistry Faculty of EngineeringKinki University Hokoku Ind.Co.Ltd. Tosoh Co.Ltd. Department of Applied Chemistry Graduate School of EngineeringHiroshima University Department of Development Science Graduate School for International Development and CooperationHiroshima University

The analysis of seven aliphatic carboxylic acids(formic,acetic,propionic,iso-butyric,n-butyric,iso-valeric and n-valeric acid) in anaerobic digestion process waters for biogas production was examined by ion-exclusion chromatography with dilute acidic eluents(benzoic acid,perfluorobutyric acid(PFBA) and sulfuric acid) and non-suppressed conductivity/ultraviolet(UV) *** columns used were a styrene/divinylbenzene-based strongly acidic cation-exchange resin column(TSKgel SCX) and a polymethacrylate-based weakly acidic cation-exchange resin column(TSKgel Super IC-A/C).Good separation was performed on the TSKgel SCX in shorter retention *** the TSKgel Super IC-A/C,peak shape of the acids was sharp and symmetrical in spite of longer retention *** addition,the mutual separation of the acids was good except for iso-and n-butyric *** better separation and good detection was achieved by using the two columns(TSKgel SCX and TSKgel Super IC-A/C connected in series),lower concentrations of PFBA and sulfuric acid as eluents,non-suppressed conductivity detection and UV detection at 210 *** analysis was applied to anaerobic digestion process *** chromatograms with conductivity detection were relatively simpler compared with those of UV *** use of two columns with different selectivities for the aliphatic carboxylic acids and the two detection modes was effective for the determination and identification of the analytes in anaerobic digestion process waters containing complex matrices.

关键词： ion-exclusion chromatography(IEC) strongly and weakly acidic cation-exchange resin columns perfluorobutyric and sulfuric acids eluents non-suppressed conductivity and ultraviolet(UV) detections aliphatic carboxylic acids anaerobic digestion process waters

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噪声暴露对全身振动感知阈值的影响(英文)

华南理工大学学报（自然科学版） 2007年第B10期35卷 108-111页

作者：佐藤哲身前田節雄矢野隆 Hokkai Gakuen University Sapporo 064-0926Japan Japan NIOSH Kawasaki 214-8585Japan Kumamoto University Kumamoto 860-8555Japan

尽管对全身振动感知阈值已有人做过一些试验研究,但是对于暴露于噪声条件下的感知阈值尚无相关试验.但此项研究是很重要的,因为许多振动源往往同时也激发产生噪声.文中通过一项试验来探讨噪声对身体振动感知阈值的影响.共有10位受试者... 详细信息

尽管对全身振动感知阈值已有人做过一些试验研究,但是对于暴露于噪声条件下的感知阈值尚无相关试验.但此项研究是很重要的,因为许多振动源往往同时也激发产生噪声.文中通过一项试验来探讨噪声对身体振动感知阈值的影响.共有10位受试者坐在固定于垂直振动台的椅子上,被要求自行调节振动级来确定阈值.采用4种正弦波形的振动以及随机振动作为刺激,同时采用以10dB作为调节量的随机噪声作为刺激.试验发现阈值随着噪声级的增加而增加.这意味着随噪声级的增加,人体对振动的敏感度下降了.尽管此中的原图尚不明晰,但文中对同一频率下听觉与振动感觉之间的相互影响提出了建议.

关键词：全身振动感知阈值噪声与振动联合作用心理试验

卫星合成孔径雷达数据在堆石坝正常和震后工况变形监测中应用

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大坝与安全 2019年第4期33卷 70-74页

作者： Hiroyuki sato takashi Sasaki Masafumi Kondo 邱祥兴日本国家土地和基础设施管理研究院河流所

为了开发高效、先进的堆石坝正常运行工况下和遭遇地震后的变形监测方法,对基于卫星合成孔径雷达(SAR)数据的监测方法进行研究。卫星SAR不需要在坝面上安装任何传感器,可以对堆石坝的整个表面进行观测,且无论天气状况如何都能够获得数据... 详细信息

为了开发高效、先进的堆石坝正常运行工况下和遭遇地震后的变形监测方法,对基于卫星合成孔径雷达(SAR)数据的监测方法进行研究。卫星SAR不需要在坝面上安装任何传感器,可以对堆石坝的整个表面进行观测,且无论天气状况如何都能够获得数据,可用于堆石坝变形监测。利用卫星SAR数据对某堆石坝近4年来的外部变形进行了评价。该坝坝顶有3个观测点,对使用卫星SAR数据得到的外部变形结果和各测点观测成果进行对比,结果表明使用卫星SAR数据得到的外部变形结果与实际调查结果吻合,外部变形平均误差约为几毫米。此外,利用卫星SAR数据研究了2016年熊本地震对堆石坝的损伤,表明卫星SAR数据可以探测地震作用下堆石坝的小变形。

关键词：卫星合成孔径雷达数据堆石坝变形监测地震