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Present Status of the Nd:YAG Thomson Scattering System Development for Time Evolution Measurement of Plasma Profile on Heliotron J

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Plasma Science and Technology 2013年第3期15卷 240-243页

作者： Takashi MINAMI Shohei ARAI Naoki KENMOCHI Hiroaki YASHIRO Chihiro takahashi Shinji KOBAYASHI Tohru MIZUUCHI Shinsuke OHSHIMA satoshi YAMAMOTO Hiroyuki OKADA Kazunobu NAGASAKI Yuji NAKAMURA Kiyoshi HANATANI Shigeru KONOSHIMA Fumimichi SANO Institute of Advanced Energy Kyoto University Graduate School of Energy Science Kyoto University Pioneering Research Unit for Next Generation Kyoto University

A new high repetition rate Nd：YAG Thomson scattering system is developed for the Heliotron J helical device. A main purpose of installing the new system is the temporal evolution measurement of a plasma profile for improved confinement physics such as the edge transport barrier （H-mode） or the internal transport barrier of the helical plasma. The system has 25 spatial points with -10 mm resolution. Two high repetition Nd：YAG lasers （〉 550 m J@ 50 Hz） realize the measurement of the time evolution of the plasma profile with ~10 ms time intervals. Scattered light is collected by a large concave mirror （D----800 mm, f/2.25） with a solid angle of -100 mstr and transferred to interference filter polychromators by optical fiber bundles in a staircase form. The signal is amplified by newly designed fast preamplifiers with DC and AC output, which reduces the low frequency background noise. The signals are digitized with a multi-event QDC, fast gated integrators. The data acquisition is performed by a VME-based system operated by the CINOS.

关键词： plasma diagnostic Nd：YAG laser Thomson scattering measurement plasma improved confinement

Intramedullary Spinal Cord Metastases in Breast Cancer: Report a Case

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Journal of Cancer Therapy 2016年第13期7卷 1034-1038页

作者： Masahiro Kitada Nana takahashi Shunsuke Yasuda satoshi Okazaki Kei Ishibashi satoshi Hayashi Department of Breast Disease Center Asahikawa Medical University Asahikawa Japan

Background: Intramedullary spinal cord metastasis (ISCM) from breast cancer is a relatively rare disease. We present this disease. Case presentation: The patient was a 67-year-old woman with lung metastasis appearing 3 years after breast cancer surgery. Complete remission was achieved for the metastatic lesion with chemotherapy, but multiple cerebellar metastases were found 3 months after the completion of chemotherapy. Whole-brain irradiation was administered, resulting in symptomatic improvement. Approximately 6 months later, the patient experienced weakness in the lower extremities and difficulty walking. Magnetic resonance imaging detected a well-defined intraspinal tumor measuring 13 × 13 × 30 mm at the level of Th12-L1. After 20-Gy irradiation to the tumor, oral steroid administration, and rehabilitation, the patient regained the ability to walk. Eight months have passed, to date, since these interventions and the patient is currently receiving treatment for metastases to bones, including the spine, but is still capable of walking without difficulty. We herein report this case with a review of the relevant literature. Conclusion: ISCM in the breast cancer is relatively rare. But, it is the clinical condition which it should always place in the mind.

关键词： Breast Cancer Intramedullary Spinal Cord Metastasis (Iscm) Brain Metastasis

Origin of Candida albicans in Human Oral Cavity

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Open Journal of Stomatology 2022年第4期12卷 137-145页

作者： Akira Fukatsu Osamu Tsuzukibashi Mana Fuchigami Yoshinori Ono satoshi Uchibori Yuji takahashi Chiaki Komine Koji Umezawa Sachiyo Hayashi Takashi Asano Taira Kobayashi Masanobu Wakami Hiroshi Murakami Masahiko Fukumoto Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry Nihon University Graduate School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: Candida albicans is regarded as a part of normal flora in the human oral cavity. However, it remains unclear whether the genus Candida, especially C. albicans, is an oral resident microorganism and causes marital infection or not. The purpose of the present study was to elucidate the origin of oral C. albicans by investigating the colonization and infection route to oral cavities of this organism with arbitrarily primed polymerase chain reaction (AP-PCR). Methods: After C. albicans was isolated from four subjects (average age: 42.2, range: 33 - 56), the isolations of this organism from them were performed six months later again. To investigate whether C. albicans is an oral resident microorganism, the genotype homology of each C. albicans isolates that were isolated twice from the same subjects was compared. Moreover, C. albicans was isolated from five pairs of married couples (average period of cohabitation: 12.4 years, range: 5 - 31). To investigate whether C. albicans causes marital infection, the genotype homology of C. albicans isolates that were isolated from each pair of married couples was compared. Results: AP-PCR patterns of C. albicans that were isolated from each subject at o month and after 6 months showed the identical genotypes among each individual. C. albicans isolates from five pairs of married couples showed the identical genotypes between a husband and wife of each pair on AP-PCR. Conclusion: These results indicated that C. albicans was an oral resident microorganism and caused the marital infection.

关键词： Candida albicans Oral Cavity AP-PCR Genotyping

Clinicopathological Study of Neuroendocrine Carcinoma of the Breast

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Journal of Cancer Therapy 2017年第8期8卷 793-799页

作者： Masahiro Kitada Shunsuke Yasuda Masahiro Abe Nana takahashi satoshi Okazaki Kei Ishibashi satoshi Hayashi Department of Breast Disease Center Asahikawa Medical University Asahikawa Japan

Introduction: Neuroendocrine carcinoma (NEC) occurs mostly in the digestive organs, such as the pancreas and gastrointestinal tract, and the lungs. Primary NEC of the breast is a rare entity. There are not many studies on this disease, and this study was carried out in order to know the clinical pathology of this disease. Methods: Of 2354 patients who underwent surgery for breast cancer in our hospital during the period from January 2000 to July 2015, 8 (0.34%) were pathologically diagnosed with NEC of the breast. This study is retrospectively registered. Result: The histological types were small cell carcinoma in one patient and invasive ductal carcinoma (IDC) with neuroendocrine differentiation in the remaining 7. The mean patient age was slightly higher than that of patients with usual IDC. On pathological examination, the NEC tumors were more hormone dependent and often HER2 negative, as compared to usual IDC, though there was no significant difference in nuclear grade. The patients with NEC of the breast showed positivity for neuroendocrine markers on immunohistological staining, and had favorable outcomes. Conclusion: NEC of the breast is rare, and there is no established consensus regarding the pathological significance, prognosis, and treatment method. Further accumulation of cases is necessary.

关键词： Breast Cancer Neuroendocrine Carcinoma

Surgical Treatment of Idiopathic Multiple Pulmonary Arteriovenous Malformation Identified at the Onset of Cerebral Infarction: A Case Report

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Open Journal of Respiratory Diseases 2017年第2期7卷 62-67页

作者： Masahiro Kitada Shunsuke Yasuda Nana takahashi satoshi Okazaki Kei Ishibashi satoshi Hayashi Yoshinobu Ohsaki Department of Respiratory Center Asahikawa Medical University Hokkaido Japan

Pulmonary arteriovenous malformation (PAVM), which is asymptomatic in most cases, is often identified in patients with central nervous system disorders such as brain abscesses and/or cerebral infarctions. We have reported a patient with idiopathic multiple PAVM identified at the onset of cerebral infarction. A 69-year-old woman visited the Department of Neuropathic Internal Medicine at our hospital with chief complaints of numbness in her left hand and a feeling of weakness. The patient was given a diagnosis of subacute cerebral infarction. Multiple old lacunar infarctions were also observed in the deep white matter of the left frontal lobe. Chest computed tomography showed multiple nodular structures, mainly in the right lower lung field (S8), as well as continuous arteries and veins at the site;thus, the patient was finally diagnosed with multiple PAVM. Right lower thoracoscopic lobectomy was performed, as is typical surgical practice in such cases. The patient had a favorable postoperative course, and had no recurrence of cerebral infarction. Although the patient’s lesions were mainly restricted to S8, the fact that there were multiple lesions deemed a lobectomy as the appropriate course of treatment. This case emphasizes that attention should be paid to cases of multiple PAVM since cerebral infarction may arise from the disease.

关键词： Pulmonary Arteriovenous Malformation (PAVM) Brain Abscesses Cerebral Infarctions

Study on Distribution of Acinetobacter baumannii Complex in Dental Hospital Using Multiplex PCR

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Open Journal of Stomatology 2023年第7期13卷 212-221页

作者： Akira Fukatsu Osamu Tsuzukibashi Hiroshi Yamamoto Yuji takahashi Keisuke Usuda Mana Fuchigami Chiaki Komine satoshi Uchibori Koji Umezawa Sachiyo Hayashi Takashi Asano Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Department of Laboratory Medicine for Dentistry for the Compromised Patient School of Dentistry Nihon University Matsudo Japan Department of Oral Surgery School of Dentistry Nihon University Matsudo Japan Department of Oral Implantology School of Dentistry Nihon University Matsudo Japan Department of Fixed Prosthodontics School of Dentistry Nihon University Matsudo Japan Department of Special Needs Dentistry School of Dentistry Nihon University Matsudo Japan

Purpose: In recent years, multidrug-resistant Acinetobacter baumannii has appeared and caused outbreaks of hospital infections all over the world. Close monitoring of this pathogen and other A. baumanii complex species is considered of critical importance to public health organizations. The reliable identification method able to distinguish A. baumannii from genetically close Acinetobacter species is needed, because these species are unable to be differentiated by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect A. baumanii complex species, and Acinetobacter lwoffii which is frequently detected from the human specimens, and to investigate the distribution of these organisms in dental hospital using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 16S ribosomal RNA (16S rRNA) gene and DNA gyrase subunit B (gyrB) of each species of A. baumanii complex species. Swab samples were collected from ten dental spittoon units in dental hospital, and the distribution of A. baumanii complex species was investigated using a multiplex PCR. Results: These primers were able to distinguish each species of A. baumanii complex species clearly. A. baumanii and A. calcoaceticus were detected at 20.0% and 10.0% in ten swab samples, respectively. On the other hand, A. nosocomialis, A. lowffii, and A. pittii were detected from no sample. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.

关键词： Acinetobacter baumannii Complex Multiplex PCR Hospital Infections

Investigation of Bacteria Species Most Involved in Peri-Implantitis

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口腔学期刊(英文) 2023年第10期13卷 353-366页

作者： Hiroshi Murakami Osamu Tsuzukibashi Akira Fukatsu Yuji takahashi Keisuke Idei Keisuke Usuda Mana Fuchigami Chiaki Komine satoshi Uchibori Koji Umezawa Sachiyo Hayashi Takashi Asano Masanobu Wakami Taira Kobayashi Masahiko Fukumoto Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: Currently, bacteriological examinations of implant treatments target periodontopathic bacteria such as red complex bacteria, including Porphyromonas gingivalis, and detect them qualitatively or quantitatively. However, it seems that those examinations do not reflect the peri-implant tissue conditions precisely, because periodontopathic bacteria are also frequently detected from healthy peri-implant sites. The purpose of the present study was to investigate bacteria species most involved in peri-implantitis using a PCR method. Methods: Polymerase chain reaction (PCR) primers in this study were designed based on partial sequences of 16S rDNA of bacteria species involved in peri-implantitis that were described in numerous previous studies. Peri-implant sulcus fluid (PISF) samples were collected from thirty periodontally healthy patients with implants (HI) and thirty patients with peri-implantitis (PI). Each detection frequency of bacteria species in PISFs of both groups was investigated using a PCR method, and was compared using Fisher’s exact test. Results: In PI group, detection frequencies of Corynebacterium durum, Fretibacterium fastidiosum and Slackia exigua were significantly higher than those of HI group (p P. gingivalis and Tannerella forsythia belonging to red complex were frequently detected in the PISF samples of HI group (p > 0.05). Conclusion: It was suggested that monitoring C. durum and F. fastidiosum levels in PISF samples was useful as a clinical indicator for the evaluation of peri-implant tissue conditions.

关键词： Peri-Implantitis PCR Method Bacteria Flora in Peri-Implant Sulcus Red Complex

One Step Multiplex PCR for Identification of Candida haemulonii complex and Candia auris

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Advances in Microbiology 2023年第12期13卷 602-613页

作者： Mana Fuchigami Osamu Tsuzukibashi Akira Fukatsu Koji Umezawa Sachiyo Hayashi Yuji takahashi Hiroshi Yamamoto Chiaki Komine Mio Hagiwara-Hamano Yukiko Iizuka satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Department of Laboratory Medicine for Dentistry for the Compromised Patient School of Dentistry at Matsudo Nihon University Chiba Japan Department of Special Needs Dentistry School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Implantology School of Dentistry at Matsudo Nihon University Chiba Japan Department of Oral Surgery School of Dentistry at Matsudo Nihon University Chiba Japan Department of Fixed Prosthodontics School of Dentistry at Matsudo Nihon University Chiba Japan

Purpose: Recently, Candida haemulonii complex (Candida haemulonii, Candida duobushaemulonii and Candida haemulonii var. vulnera) and two genetically close species (Candida pseudohaemulonii and Candida auris) have emerged as an opportunistic fungal pathogen associated with various infectious diseases of humans, and most of those isolates have displayed antifungal resistance. Because it is difficult to differentiate these microorganisms by a current technique, unfortunately, it is important to establish a method for identifying them accurately. The purpose of the present study was to design species-specific primers in order to identify and detect C. auris, C. pseudohaemulonii, and C. haemulonii complex, i.e. , C. haemulonii, C. duobushaemulonii and C. haemulonii var. vulnera using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the 26S rRNA, 18S rRNA, and RPB1 genes and ITS region of five Candida species. Results: The multiplex PCR method developed in this study was able to distinguish five Candida species clearly. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction.

关键词： Genus Candida Candida haemulonii complex Multiplex PCR Candida auris

Isolation and Identification Methods for Oral Klebsiella pneumoniae Involved in Onset of Inflammatory Bowel Disease

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Open Journal of Stomatology 2023年第7期13卷 197-211页

作者： Osamu Tsuzukibashi Akira Fukatsu Hiroshi Yamamoto Yuji takahashi Keisuke Usuda Mana Fuchigami Chiaki Komine satoshi Uchibori Koji Umezawa Sachiyo Hayashi Takashi Asano Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Department of Laboratory Medicine for Dentistry for the Compromised Patient School of Dentistry Nihon University Matsudo Japan Department of Oral Surgery School of Dentistry Nihon University Matsudo Japan Department of Oral Implantology School of Dentistry Nihon University Matsudo Japan Department of Fixed Prosthodontics School of Dentistry Nihon University Matsudo Japan Department of Special Needs Dentistry School of Dentistry Nihon University Matsudo Japan

Purpose: Recently, it was reported that Klebsiella pneumoniae is related to the onset of inflammatory bowel disease including the Crohn disease. It was frequently reported that K. pneumoniae was detected in human oral cavities. Regrettably, it currently remains unclear whether K. pneumoniae is part of the normal oral flora. The aim of this study was to establish the isolation and identification methods for K. pneumoniae from human oral cavities, and investigate its transmission pattern. Methods: A selective medium, OKPSM, for the isolation of K. pneumoniae from oral cavities was developed in this study. Also, PCR primer for the identification and detection at subspecies level of K. pneumoniae was designed. Results: OKPSM and PCR method using the primers designed in this study were useful for the isolation and identification of K. pneumoniae from human oral cavities. K. pneumoniae subsp. pneumoniae was detected at 10.0% in 30 saliva samples. On the other hand, K. pneumoniae subsp. ozaenae and K. pneumoniae subsp. rhinoscleromatis were detected from no sample. Moreover, K. pneumoniae subsp. pneumoniae isolates from same subject at 0 month and after 3 months showed same genotypes on AP-PCR using OPA-07 primer. Conclusion: These results indicated that human oral cavities were not suitable for the habitat of K. pneumoniae.

关键词： Klebsiella pneumoniae Selective Medium Oral Cavity Multiplex PCR