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Cortical transcriptome analysis after spinal cord injury reveals the regenerative mechanism of central nervous system in CRMP2 knock-in mice

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Neural Regeneration Research 2021年第7期16卷 1258-1265页

作者： Ayaka Sugeno Wenhui Piao Miki Yamazaki Kiyofumi takahashi koji Arikawa Hiroko Matsunaga Masahito Hosokawa Daisuke Tominaga Yoshio Goshima Haruko Takeyama Toshio Ohshima Laboratory for Molecular Brain Science Department of Life Science and Medical BioscienceGraduate School of Advanced Science and EngineeringWaseda UniversityTokyoJapan Biomolecular Engineering Laboratory Department of Life Science and Medical BioscienceGraduate School of Advanced Science and EngineeringWaseda UniversityTokyoJapan Computational Bio Big-Data Open Innovation Laboratory(CBBD-OIL) National Institute of Advanced Industrial Science and Technology(AIST)TokyoJapan Research Organization for Nano and Life Innovation Waseda UniversityTokyoJapan Institute for Advanced Research of Biosystem Dynamics Waseda Research Institute for Science and EngineeringGraduate School of Advanced Science and EngineeringWaseda UniversityTokyoJapan Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology(AIST)TsukubaJapan Department of Molecular Pharmacology and Neurobiology Graduate School of MedicineYokohama City UniversityYokohamaJapan

Recent studies have shown that mutation at Ser522 causes inhibition of collapsin response mediator protein 2(CRMP2) phosphorylation and induces axon elongation and partial recovery of the lost sensorimotor function after spinal cord injury(SCI).We aimed to reveal the intracellular mechanism in axotomized neurons in the CRMP2 knock-in(CRMP2KI) mouse model by performing transcriptome analysis in mouse sensorimotor cortex using micro-dissection punching system.Prior to that, we analyzed the structural pathophysiology in axotomized or neighboring neurons after SCI and found that somatic atrophy and dendritic spine reduction in sensorimotor cortex were suppressed in CRMP2KI mice.Further analysis of the transcriptome has aided in the identification of four hemoglobin genes Hba-a1, Hba-a2, Hbb-bs, and Hbb-bt that are significantly upregulated in wild-type mice with concomitant upregulation of genes involved in the oxidative phosphorylation and ribosomal pathways after SCI.However, we observed substantial upregulation in channel activity genes and downregulation of genes regulating vesicles, synaptic function, glial cell differentiation in CRMP2KI mice.Moreover, the transcriptome profile of CRMP2KI mice has been discussed wherein energy metabolism and neuronal pathways were found to be differentially regulated.Our results showed that CRMP2KI mice displayed improved SCI pathophysiology not only via microtubule stabilization in neurons, but also possibly via the whole metabolic system in the central nervous system, response changes in glial cells, and synapses.Taken together, we reveal new insights on SCI pathophysiology and the regenerative mechanism of central nervous system by the inhibition of CRMP2 phosphorylation at Ser522.All these experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Waseda University, Japan(2017-A027 approved on March 21, 2017;2018-A003 approved on March 25, 2018;2019-A026 approved on March 25, 2019).

关键词： CNS regeneration cortex CRMP2 hemoglobin metabolic pathway spinal cord injury spine transcriptome

Russell body duodenitis in a patient with retroperitoneal metastasis of ureteral cancer

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World Journal of Gastroenterology 2013年第1期19卷 125-128页

作者： Yoshihisa takahashi Seiichiro Shimizu koji Uraushihara Toshio Fukusato Department of Pathology Teikyo University School of MedicineTokyo 173-8605Japan Department of Pathology Showa General HospitalTokyo 187-8510Japan Department of Gastrointestinal Medicine Showa General HospitalTokyo 187-8510Japan

Russell bodies are globular and eosinophilic inclusion bodies in the cytoplasm of mature plasma cells.Plasma cells whose cytoplasm is filled with Russell bodies are designated as Mott cells.Russell body duodenitis(RBD) is a unique form of chronic duodenitis that is characterized by infiltration of numerous Mott cells.RBD is very rare;only two cases have been reported to date.In this paper,we report a case of RBD in a patient with retroperitoneal metastasis of ureteral cancer.A 77-year-old man was admitted to our hospital complaining of appetite loss,vomiting,and upper abdominal distension.He had undergone left nephroureterectomy for ureteral cancer 4 years earlier.Upper digestive tract endoscopy revealed edema,stenosis,and punctate redness of the mucosa of the duodenum,and a biopsy was performed.Histological analysis showed that numerous Mott cells had infiltrated the lamina propria mucosae,and the condition was diagnosed as RBD.A mass lesion in the retroperitoneum adjacent to the duodenum was detected by abdominal computed tomography,and was diagnosed as metastatic urothelial carcinoma by biopsy.It is possible that chemokines produced by tumor cells caused RBD in this case.

关键词： Russell body Duodenitis Ureteral cancer Mott cells Plasma cells

Elevated plasma cryofibrinogen in patients with active inflammatory bowel disease is morbigenous

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World Journal of Gastroenterology 2006年第10期12卷 1621-1625页

作者： koji Sawada Ryouki takahashi Abbi R Saniabadi Maiko Ohdo Takashi Shimoyama Department of Gastroenterology Fujimoto Hospital MedicineOsaka and Department of GastroenterologyHyogo College of MedicineNishinomiyaJapan Wakamoto Pharmaceuticals Sagami Laboratory KanagawaJapan Japan Immunoresearch Laboratories.Takasaki Japan Department of Gastroenterology Hyogo College of Medicine NishinomiyaJapan

瞄准：在活跃的煽动性的肠疾病(IBD ) 调查冷沉纤维蛋白(CF ) 的角色。方法：CF 是在 284 个题目的 assayed：61 与活跃并且 63 与不活跃的 ulcerative (UC ) ， 45 有 proctocolectomy， 35 与活跃并且 20 与不活跃的 Crohn 的疾病(CD... 详细信息

瞄准：在活跃的煽动性的肠疾病(IBD ) 调查冷沉纤维蛋白(CF ) 的角色。方法：CF 是在 284 个题目的 assayed：61 与活跃并且 63 与不活跃的 ulcerative (UC ) ， 45 有 proctocolectomy， 35 与活跃并且 20 与不活跃的 Crohn 的疾病(CD ) ， 40 与另外的疾病和 20 健康控制。胰岛素禁止者(TI ) 和 TI 抗体(TI-Ab ) 被 ELISA 在血浆和 CF 建筑群测量。结果：在活跃 UC 的 CF 与所有另外的组(c2<0.001 ) 相比惊人地高。同样， CF 比在不活跃的 CD 或在控制(c2<0.01 ) 在活跃 CD 是显著地更高的。在 UC，高 CF 和 TI-Ab 与对操作的需要被联系。进一步，在血浆的高 CF， CF/fibrinogen 比率，低 TI 和高 TI-Ab 与疾病活动或倔强被联系到药。提高的 CF 没与相似 C 反应的蛋白质和白血房间除了红细胞沉降率数的尖锐反应物被联系，建议那提高的 CF 不是急性炎的后果。结论：在活跃 IBD 的提高的 CF 看起来是致病。CF 经由二主要机制支持 IBD， TI (一种反煽动性的物质) 熄灭并且损害由形成蛋白质总数的微脉管的灌注。CF 可以也用作长期的 IBD 的一个简历标记。另外的研究被保证充分在 IBD 评估 CF 的角色，结果应该贡献 IBD 的致病的更好的理解。

关键词：等离子体冷纤维蛋白原疾病炎症药物抗体

Unfamiliar waveforms spanning from the ST to TP segments only observed in certain limb leads of the standard 12-lead electrocardiogram due to Aslanger’ s sign

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老年心脏病学杂志:英文版 2023年第9期20卷 693-696页

作者： koji takahashi Nobuhisa Yamamura Mako Yoshino Daijiro Enomoto Hiroe Morioka Shigeki Uemura Takafumi Okura Tomoki Sakaue Shuntaro Ikeda Department of Community Emergency Medicine Ehime University Graduate School of MedicineEhimeJapan Department of Cardiology Yawatahama City General HospitalEhimeJapan Department of Clinical Pathology Yawatahama City General HospitalEhimeJapan

Aslanger’s sign,also known as the arterial pulse tapping artifact or electromechanical association artifact,is an electrocardiographic artifact caused by arterial pulsation at the site where the limb leads of the standard 12-lead electrocardiogram near the radial or posterior tibial arteries are positioned,particularly in hyperdynamic states.[1–8]It occurs in every cardiac cycle with a constant coupling interval between the QRS complex and artifact.This synchronization with the underlying heart rhythm makes it less likely to be recognized as an artifact compared to unsynchronized artifacts,such as those caused by limb movement and inadequate contact between the electrode and skin.[1,2,7,8]Almost all reported cases of Aslanger’s sign exhibit an unusual waveform morphology in all 12 leads except one of the standard 12-lead electrocardiogram.This sign is often confused with an electrocardiographic finding commonly observed during acute coronary events.

关键词： electrocardiogram arterial likely

Fragmented QRS complex with an additional R-wave attenuated by short RR interval in a patient with acute pulmonary embolism and cardiogenic shock

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Journal of Geriatric Cardiology 2022年第12期19卷 995-1000页

作者： koji takahashi Hiromasa Nakahara Eiji Arimitsu Satoshi Imamine Yoshiyasu Obata Kimio Nakanishi Yoshiyasu Taniguchi Maiko Amano Chika Omori Takafumi Okura Department of Community Emergency Medicine Ehime University Graduate School of MedicineEhimeJapan Department of Cardiology Yawatahama City General HospitalEhimeJapan Department of Internal Medicine Oozu City HospitalEhimeJapan Department of Clinical Laboratory Oozu City HospitalEhimeJapan

Registries and hospital discharge datasets of unselected patients with pulmonary embolism show 30-day all-cause mortality rates of approximately 10%.[1]Clinical findings from histories and physical examinations,such as age,systemic blood pressure,and respiratory rate at presentation,classify patients into risk classes of increasing risk of death and other adverse medical outcomes.

关键词： mortality patients Clinical

Photosynthesis-Dependent Extracellular Ca2+ Influx Triggers an Asexual Reproductive Cycle in the Marine Red Macroalga Porphyra yezoensis

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American Journal of Plant Sciences 2010年第1期1卷 1-11页

作者： Megumu takahashi Naotsune Saga koji Mikami Graduate School of Fisheries Sciences Hokkaido University Faculty of Fisheries Sciences Hokkaido University

Asexual propagation to increase the number of gametophytic clones via the growth of asexual haploid spores is a unique survival strategy found in marine multicellular algae. However, the mechanisms regulating the asexual life cycle are largely unknown. Here, factors involved in the regulation of production and discharge of asexual spores, so-called monospores, are identified in the marine red macroalga Porphyra yezoensis. First, enhanced discharge of monospores was found by incubation of gametophytes in ASPMT1, a modified version of the previously established synthetic medium ASP12. Comparison of the compositions of ASPMT1 and our standard medium, ESL, indicated that the Ca2+ concentration in ASPMT1 was three times lower than that in ESL medium. Thus, we modified ASPMT1 by increasing its Ca2+ concentration, resulting in reduction of monospore discharge. These findings demonstrate the role of reduced Ca2+ concentrations in enhancing monospore production and release. Moreover, it was also observed that initiation of asexual life cycle required illumination, was repressed by DCMU, and was induced by a Ca2+ ionophore in the dark. Taken together, these results indicate that photosynthesis-dependent Ca2+ influx triggers the asexual life cycle by promoting the production and discharge of monospores in P. yezoensis.

关键词： Asexual Life Cycle Bangiophyceae Ca2+ Influx Monospore Photosynthesis Porphyra yezoensis Rhodophyta Synthetic Medium

Predictive factors for body weight loss and its impact on quality of life following gastrectomy

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World Journal of Gastroenterology 2017年第26期23卷 4823-4830页

作者： Kazuaki Tanabe Masazumi takahashi Takashi Urushihara Yoichi Nakamura Makoto Yamada Sang-Woong Lee Shinnosuke Tanaka Akira Miki Masami Ikeda koji Nakada Department of Gastroenterological Surgery Hiroshima University Hospital Division of Gastroenterological Surgery Yokohama Municipal Citizen’s Hospital Department of Gastroenterological Breast and Transplant SurgeryHiroshima Prefectural Hospital Department of Surgery Toho University Ohashi Medical Center Department of Surgery Gifu Municipal Hospital Department of General and Gastroentero-logical Surgery Osaka Medical College Department of Gastroenterological Surgery Fukuoka University School of Medicine Department of Surgery Kobe City Medical Center General Hospital Department of Surgery Asama General Hospital Department of Laboratory Medicine the Jikei University School of Medicine

AIM To determine the predictive factors and impact of body weight loss on postgastrectomy quality of life(QOL). METHODS We applied the newly developed integrated questionnaire postgastrectomy syndrome assessment scale-45, which consists of 45 items including those from the Short Form-8 and Gastrointestinal Symptom Rating Scale instruments, in addition to 22 newly selected items. Between July 2009 and December 2010, completed questionnaires were received from 2520 patients with curative resection at 1 year or more after having undergone one of six types of gastrectomy for Stage Ⅰ gastric cancer at one of 52 participating institutions. Of those, we analyzed 1777 eligible questionnaires from patients who underwent total gastrectomy with Roux-en-Y procedure(TGRY) or distal gastrectomy with Billroth-I(DGBI) or Roux-en-Y(DGRY) procedures. RESULTS A total of 393, 475 and 909 patients underwent TGRY, DGRY, and DGBI, respectively. The mean age of patients was 62.1 ± 9.2 years. The mean time interval between surgery and retrieval of the questionnaires was 37.0 ± 26.8 mo. On multiple regression analysis, higher preoperative body mass index, total gastrectomy, and female sex, in that order, were independent predictors of greater body weight loss after gastrectomy. There was a significant difference in the degree of weight loss(P 25 kg/m2). Multiple linear regression analysis identified lower postoperative body mass index, rather than greater body weight loss postoperatively, as a certain factor for worse QOL(P < 0.0001) after gastrectomy, but the influence of both such factors on QOL was relatively small(R2, 0.028-0.080).CONCLUSION While it is certainly important to maintain adequate body weight after gastrectomy, the impact of body weight loss on QOL is unexpectedly small.

关键词：生活的质量 Gastrectomy 重量损失 Postgastrectomy 症候群评价 scale-45

Background factors influencing postgastrectomy syndromes after various types of gastrectomy

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World Journal of Clinical Cases 2018年第16期6卷 1111-1120页

作者： Shinichi Kinami Masazumi takahashi Takashi Urushihara Masami Ikeda Masashi Yoshida Yoshikazu Uenosono Atsushi Oshio Yoshimi Suzukamo Masanori Terashima Yasuhiro Kodera koji Nakada Department of Surgical Oncology Kanazawa Medical University Division of Gastroenterological Surgery Yokohama Municipal Citizen’s Hospital Department of Gastroenterological Breast and Transplant Surgery Hiroshima Prefectural Hospital Department of Surgery Asama General Hospital Department of Surgery International University of Health and Welfare Hospital Department of Surgery Imamura General Hospital Faculty of Letters Arts and Sciences Waseda University Department of Physical Medicine and Rehabilitation Tohoku University Graduate School of Medicine Division of Gastric Surgery Shizuoka Cancer Center Department of Gastroenterological Surgery Nagoya University Graduate School of Medicine Department of Laboratory Medicine The Jikei University Daisan Hospital

BACKGROUND Postgastrectomy syndromes(PGS) after curative gastrectomy for gastric cancer are influenced by not only gastrectomy type but also by background factors. Recently, a nationwide PGS study was performed using the Postgastrectomy Syndrome Assessment Scale-45(PGSAS-45) questionnaire.AIM To determine the influence of each background factor on PGS for each gastrectomy type using PGS assessment study(PGSAS) data as an additional analysis. METHODS The data of 2368 patients were obtained from the PGSAS. This included patients undergoing distal gastrectomy(DG) with Billroth I reconstruction, DG with Roux-en-Y reconstruction, total gastrectomy with Roux-en-Y, proximal gastrectomy, pylorus-preserving gastrectomy(PPG), and local resection. Multiple regression analysis was performed to explore the independent effects of each background factor on the main outcome measures(MOMs) of PGSAS-45 for each gastrectomy type. The background factors included postoperative period, age, sex, surgical approach(laparoscopic or open), and the status of the celiac branch of the vagal nerve.RESULTS The MOMs of DG and PPG were highly affected by background factors, whereas those of total gastrectomy with Roux-en-Y, proximal gastrectomy, and local resection were not. Worse PGS were found in females, whereas a longer postoperative period alleviated some of the MOMs. For DG and PPG, a laparoscopic approach and preservation of the celiac branch improved several MOMs.CONCLUSION Various background factors affected PGS, and their influence varied with the type of gastrectomy performed. Laparoscopic surgery and celiac branch preservation can improve PGS in patients undergoing DG and PPG.

关键词： Postgastrectomy syndrome Gastrectomy Gastric cancer Postgastrectomy Syndrome Assessment Scale-45

Serum cystatin C can be used as a marker of renal function even in patients with intestinal urinary diversion

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Asian Journal of Urology 2015年第3期2卷 167-169页

作者： Masahiro Matsuki Toshiaki Tanaka Takeshi Maehana koji Ichihara Masahiro Yanase Masanori Matsukawa Hideki Adachi Satoshi takahashi Naoya Masumori Department of Urology Sapporo Medical UniversitySchool of MedicineSapporoJapan Sapporo Kidney Disease Treatment Forum SapporoJapan Department of Urology Sunagawa City Medical CenterSunagawaJapan Department of Urology Takikawa Municipal HospitalTakikawaJapan Department of Urology SaiseikaiOtaru HospitalOtaruJapan

Objective:Recently,serum cystatin C(CysC)has been used as a novel marker of renal function.However,there is a lack of data on CysC levels in patients with intestinal urinary diversion(UD).Here we report CysC levels in such patients.Methods:We prospectively observed 38 patients who were diagnosed with bladder cancer and subsequently treated with radical cystectomy and UD at our institution in 2012 and 2013.Serum creatinine(sCr)and CysC were obtained optionally at the same time at least 1 month after radical cystectomy and UD.Results:The median CysC and sCr concentrations were 1.12 mg/L(range 0.75-2.47 mg/L)and 0.99 mg/dL(range 0.61-2.22 mg/dL),respectively.The median estimated concentrations of glomerular filtration rate(GFR)based on CysC(eGFRcys)and GFR based on creatinine(eGFRcreat)were 61.08 mL/min/1.73 m^2(range 22.64e99.89 mL/min/1.73 m^2)and 58.01 mL/min/1.73 m^2(range 23.48e91.82 mL/min/1.73 m2),respectively.CysC had a significant correlation with sCr(r=0.8607,p<0.0001)and eGFRcreat(r=-0.8993,p<0.0001).eGFRcys also had a significant correlation with eGFRcreat(r=0.8104,p<0.0001).Conclusion:The correlation between CysC and sCr was strong and the correlation coefficient was equivalent to that in patients without UD.The results suggest that CysC is not affected by UD and can be used as a marker of renal function similarly to sCr in patients with UD.

关键词： Renal function Cystatin C Urinary diversion