检索结果-南通市图书馆

Beyond ribosomal function:RPS6 deficiency suppresses cholangiocarcinoma cell growth by disrupting alternative splicing

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第9期14卷 3931-3948页

作者： Wenkang Fu Yanyan Lin Mingzhen Bai Jia Yao Chongfei Huang Long gao Ningning Mi Haidong Ma Liang Tian ping Yue Yong Zhang Jinduo zhang Yanxian Ren Liyun Ding Lunzhi Dai Joseph WLeung Jinqiu Yuan Wenhua Zhang Wenbo Meng The First School of Clinical Medicne Lanzhou UniversityLanzhou 730030China Department of General Surgery the First Hospital of Lanzhou UniversityLanzhou 730030China Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province the First Hospital of Lanzhou UniversityLanzhou 730000China School of Physical Science and Technology Lanzhou UniversityLanzhou 730000China National Clinical Research Center for Geriatrics and Department of General Practice State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Division of Gastroenterology UC Davis Medical Center and Sacramento VA Medical CenterSacramentoCA 95817USA Clinical Research Center Big Data Centerthe Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China School of Life Sciences Lanzhou UniversityLanzhou 730000China

Cholangiocarcinoma(CCA)is a bile duct malignancy with a dismal *** study systematically investigated the role of the ribosomal protein S6(RPS6)gene,which is dependent in *** found that RPS6 upregulation in CCA tissues was correlated with a poor *** investigations have shown that alterations in RPS6 expression,both gain-and loss-of function could affect the proliferation of CCA *** xenograft tumor models,RPS6 overexpression enhances tumorigenicity,whereas RPS6 silencing reduces *** analysis using RNA-seq and proteomics elucidated downstream signaling pathways of RPS6 depletion by affecting the cell cycle,especially DNA *** followed by mass spectrometry has identified numerous spliceosome complex proteins associated with *** profiling revealed that RPS6 affects numerous alternative splicing(AS)events,and combined with RNA immunoprecipitation sequencing,revealed that minichromosome maintenance complex component 7(MCM7)binds to RPS6,which regulates its AS and increases oncogenic activity in *** RPS6 with vivo phosphorodiamidate morpholino oligomer(V-PMO)significantly inhibited the growth of CCA cells,patient-derived organoids,and subcutaneous xenograft *** together,the data demonstrate that RPS6 is an oncogenic regulator in CCA and that RPS6-V-PMO could be repositioned as a promising strategy for treating CCA.

关键词： Cholangiocarcinoma Ribosomal protein S6 Alternative splicing p53 Minichromosome maintenance complex component 7 Vivo morpholino Patient-derived organoids

Re含量对TiCN-WC-HfN陶瓷微观组织和力学性能的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

粉末冶金技术 2024年第1期42卷 53-58页

作者：高姣姣平萍刘家宝宋金鹏太原理工大学航空航天学院太原030024 太原重型机械集团有限公司太原030024 太原理工大学机械与运载工程学院太原030024

采用真空热压烧结技术制备了TiCN–WC–HfN陶瓷,研究了Re含量(摩尔分数)对其微观组织和力学性能的影响。结果表明:TiCN–WC–HfN–Ni–Re陶瓷材料由TiC_(0.41)N_(0.5)、WC、HfN、TiC、Ni和Re组成,其中TiC_(0.41)N_(0.5)0是TiC与TiN在烧... 详细信息

采用真空热压烧结技术制备了TiCN–WC–HfN陶瓷,研究了Re含量(摩尔分数)对其微观组织和力学性能的影响。结果表明:TiCN–WC–HfN–Ni–Re陶瓷材料由TiC_(0.41)N_(0.5)、WC、HfN、TiC、Ni和Re组成,其中TiC_(0.41)N_(0.5)0是TiC与TiN在烧结过程中生成的固溶体。TiCN–WC–HfN–Ni–Re陶瓷材料断口上存在凹坑和解理面,当Re摩尔分数为2.5%时,材料断口上的凹坑较多。当Re摩尔分数由0增到3.0%时,材料的维氏硬度、抗弯强度和断裂韧度均先增大后减小。当Re摩尔分数为2.5%时,材料的力学性能最优,其维氏硬度为(19.25±0.21)GPa、抗弯强度为(1304±23)MPa、断裂韧度为(7.73±0.22)MPa∙m^(1/2)。TiCN–WC–HfN–Ni–Re陶瓷在断裂过程中发生了穿晶断裂和沿晶断裂,其增韧机制为裂纹偏转和裂纹桥连。

关键词： TiCN–WC–HfN陶瓷铼微观组织力学性能

Relationship between Phenotypic Changes of Dendritic Cell Subsets and the Onset of Plateau Phase during Intermittent Interferon Therapy in Patients with CHB

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2024年第3期37卷 303-314页

作者： YANG Liu WANG Shi Yu JIANG Ting Ting DENG Wen CHANG Min WU Shu Ling CAO Wei Hua LU Yao SHEN Ge LIU Ru Yu gao Yuan Jiao XU Meng Jiao HU Lei ping ZHANG Lu XIE Yao LI Ming Hui Department of Hepatology Division 2 Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Center for Infectious Diseases Second Affiliated Hospital of Air Force Medical UniversityXi'an 710038ShaanxiChina Department of Hepatology Division 2 Peking University Ditan Teaching HospitalBeijing 100015China

Objective This study aimed to evaluate whether the onset of the plateau phase of slow hepatitis B surface antigen decline in patients with chronic hepatitis B treated with intermittent interferon therapy is related to the frequency of dendritic cell subsets and expression of the costimulatory molecules CD40,CD80,CD83,and *** This was a cross-sectional study in which patients were divided into a natural history group(namely NH group),a long-term oral nucleoside analogs treatment group(namely NA group),and a plateau-arriving group(namely P group).The percentage of plasmacytoid dendritic cell and myeloid dendritic cell subsets in peripheral blood lymphocytes and monocytes and the mean fluorescence intensity of their surface costimulatory molecules were detected using a flow *** In total,143 patients were enrolled(NH group,n=49;NA group,n=47;P group,n=47).The results demonstrated that CD141/CD1c double negative myeloid dendritic cell(DNmDC)/lymphocytes and monocytes(%)in P group(0.041[0.024,0.069])was significantly lower than that in NH group(0.270[0.135,0.407])and NA group(0.273[0.150,0.443]),and CD86 mean fluorescence intensity of DNmDCs in P group(1832.0[1484.0,2793.0])was significantly lower than that in NH group(4316.0[2958.0,5169.0])and NA group(3299.0[2534.0,4371.0]),Adjusted P all<*** Reduced DNmDCs and impaired maturation may be associated with the onset of the plateau phase during intermittent interferon therapy in patients with chronic hepatitis B.

关键词： CHB Dendritic Cells Intermittent Interferon Therapy Plateau Phase

基于端到端深度神经网络和图搜索的OCT图像视网膜层边界分割方法

在线全文

学校读者我要写书评

暂无评论

软件学报 2024年第6期35卷 3036-3051页

作者：胡凯蒋帅刘冬高协平智能计算与信息处理教育部重点实验室(湘潭大学) 湖南湘潭411105 智能计算与语言信息处理湖南省重点实验室(湖南师范大学) 湖南长沙410081 医学影像人工智能湖南省重点实验室(湘南学院) 湖南郴州423000

视网膜层边界的形态变化是眼部视网膜疾病出现的重要标志,光学相干断层扫描(optical coherence tomography,OCT)图像可以捕捉其细微变化,基于OCT图像的视网膜层边界分割能够辅助相关疾病的临床判断.在OCT图像中,由于视网膜层边界的形态... 详细信息

视网膜层边界的形态变化是眼部视网膜疾病出现的重要标志,光学相干断层扫描(optical coherence tomography,OCT)图像可以捕捉其细微变化,基于OCT图像的视网膜层边界分割能够辅助相关疾病的临床判断.在OCT图像中,由于视网膜层边界的形态变化多样,其中与边界相关的关键信息如上下文信息和显著性边界信息等对层边界的判断和分割至关重要.然而已有分割方法缺乏对以上信息的考虑,导致边界不完整和不连续.针对以上问题,提出一种“由粗到细”的基于端到端深度神经网络和图搜索(graph search,GS)的OCT图像视网膜层边界分割方法,避免了非端到端方法中普遍存在的“断层”现象.在粗分割阶段,提出一种端到端的深度神经网络—注意力全局残差网络(attention global residual network,AGR-Net),以更充分和有效的方式提取上述关键信息.具体地,首先设计一个全局特征模块(global feature module,GFM),通过从图像的4个方向扫描以捕获OCT图像的全局上下文信息;其次,进一步将通道注意力模块(channel attention module,CAM)与全局特征模块串行组合并嵌入到主干网络中,以实现视网膜层及其边界的上下文信息的显著性建模,有效解决OCT图像中由于视网膜层形变和信息提取不充分所导致的误分割问题.在细分割阶段,采用图搜索算法去除AGR-Net粗分割结果中的孤立区域或和孔洞等,保持边界的固定拓扑结构和连续平滑,以实现整体分割结果的进一步优化,为医学临床的诊断提供更完整的参考.最后,在两个公开数据集上从不同的角度对所提出的方法进行性能评估,并与最新方法进行比较.对比实验结果也表明所提方法在分割精度和稳定性方面均优于现有方法.

关键词： OCT图像视网膜层边界分割残差神经网络注意力图搜索

Novel compound heterozygous mutations in the hemojuvelin gene in a juvenile hemochromatosis patient:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第19期12卷 3961-3970页

作者： Ling-Ding Xie Xiao-Mu Kong Jing-Xia Shen tai-Ling Wang Jing Ma Yun-Fen Zhang Xiao-ping Chen Department of Endocrinology China-Japan Friendship HospitalBeijing 100029China Department of Tumor Radiotherapy and Chemotherapy Qian’an People’s HospitalQian’an 064400Hebei ProvinceChina Department of Pathology China-Japan Friendship HospitalBeijing 100029China Department of Endocrinology Aksu First People's HospitalAksu Region 843000Xinjiang Uygur Autonomous RegionChina Department of Metabolic Diseases Qian’an Traditional Chinese Medical HospitalQian’an 064400Hebei ProvinceChina

BACKGROUND Juvenile hemochromatosis(JH)is an early-onset,rare autosomal recessive disorder of iron overload observed worldwide that leads to damage in multiple *** mutations in the hemojuvelin(HJV)gene are the major cause of *** SUMMARY A 34-year-old male Chinese patient presented with liver fibrosis,diabetes,hypogonadotropic hypogonadism,hypophysis hypothyroidism,and skin *** test revealed a markedly elevated serum ferritin level of 4329μg/L and a transferrin saturation rate of 95.4%.Targeted exome sequencing and Sanger sequencing revealed that the proband had a novel mutation c.863G>A(p.R288Q)in the HJV gene which was transmitted from his father,and two known mutations,c.18G>C(p.Q6H)and c.962_963delGCinsAA(p.C321*)in cis,which were inherited from his *** p.R288W mutation was previously reported to be pathogenic for hemochromatosis,which strongly supported the pathogenicity of p.R288Q reported for the first time in this *** 72 wk of intensive phlebotomy therapy,the patient achieved a reduction in serum ferritin to 160.5μg/*** patient's clinical symptoms demonstrated a notable *** This study highlights the importance of screening for hemochromatosis in patients with diabetes and hypogonadotropic *** also suggests that long-term active phlebotomy could efficiently improve the prognosis in severe JH.

关键词： Juvenile hemochromatosis Liver cirrhosis Diabetes Iron overload Phlebotomy Case report

离心力作用下轴承滚道润滑剂流失与定量补油数值模拟

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

摩擦学学报 2024年第9期44卷 1184-1191页

作者：高军斌杨萍栗心明高志伟郭峰 Poll Gerhard 青岛理工大学机械与汽车工程学院德国莱布尼茨汉诺威大学机械设计与摩擦学研究所

采用自由表面薄膜流动模型，数值模拟了高速轴承壁面润滑剂在离心力作用下的流动过程，并通过外部定量补油对壁面油层厚度和润滑状态进行了调控.结果表明：高速下离心力的作用可使轴承壁面的润滑剂呈现出非线性衰退，使轴承处于乏油润... 详细信息

采用自由表面薄膜流动模型，数值模拟了高速轴承壁面润滑剂在离心力作用下的流动过程，并通过外部定量补油对壁面油层厚度和润滑状态进行了调控.结果表明：高速下离心力的作用可使轴承壁面的润滑剂呈现出非线性衰退，使轴承处于乏油润滑状态；与离心力相比，由表面张力引起的润滑剂回填几乎可以忽略，需依靠外部供油对轴承壁面油层进行增补；通过调整供油时间间隔，可实现轴承壁面润滑剂流动的供失平衡；采用多次微量供油，可将壁面油层厚度和接触区润滑状态调控在一定范围内，进而使轴承处于最佳的润滑状态.

关键词：油层衰退离心力润滑剂补充滚动轴承

来源：博看期刊

同方期刊数据库

在线全文

学校读者我要写书评

暂无评论

编辑思想:本质、研究困境及优化路径

河南大学学报（社会科学版） 2024年第5期64卷 129-136,156页

作者：高兆强吴平武汉大学信息管理学院湖北武汉430072

编辑思想是编辑工作的灵魂,编辑思想研究是编辑学研究的重要组成部分。编辑思想本质上是由编辑观念、编辑理想、编辑道德和编辑方法构成的具有主观性和客观性、历史性和即时性、个性和社会性、理论性和实践性等性质的思想系统,其间蕴含... 详细信息

编辑思想是编辑工作的灵魂,编辑思想研究是编辑学研究的重要组成部分。编辑思想本质上是由编辑观念、编辑理想、编辑道德和编辑方法构成的具有主观性和客观性、历史性和即时性、个性和社会性、理论性和实践性等性质的思想系统,其间蕴含着理性与非理性、成熟态与非成熟态、个性化彰显与社会性规制、认识本质和实践取向之间的矛盾。目前,对编辑思想的研究存在材料获取难、理解难、言说难、成果验证难等难题。树立明确的问题意识、正确地认识编辑思想、重视编辑思想产生和应用的社会环境、规范编辑思想研究范式,有助于提高编辑思想研究的科学性,帮助破解这些难题。

关键词：编辑思想编辑价值编辑学研究研究范式

饲粮纤维结构及其发酵产物对单胃动物肠道健康的调控机制和应用现状

在线全文

学校读者我要写书评

暂无评论

动物营养学报 2024年第4期36卷 2057-2066页

作者：李敬高歌李平李习龙中国农业科学院饲料研究所农业部饲料生物技术重点开放实验室北京100081 广东省农业科学院动物科学研究所广州510640 张家口市畜牧技术推广站张家口075000

饲粮纤维作为动物后段肠道微生物群最重要的食物来源,维护着微生物群的平衡和稳态,其代谢产物短链脂肪酸(SCFAs)对改善动物能量代谢、肠道屏障功能、免疫系统、炎症反应和肠激素分泌等都具有非常重要的调控作用。然而,饲粮中纤维的结构... 详细信息

饲粮纤维作为动物后段肠道微生物群最重要的食物来源,维护着微生物群的平衡和稳态,其代谢产物短链脂肪酸(SCFAs)对改善动物能量代谢、肠道屏障功能、免疫系统、炎症反应和肠激素分泌等都具有非常重要的调控作用。然而,饲粮中纤维的结构特点以及不同饲料原料中纤维的组成和发酵特性差异较大,也导致纤维性饲粮原料在动物生产中的应用存在巨大的变异性。因此,本文对饲粮纤维结构及其发酵特性进行了深入探讨,并综述了饲料纤维组分在动物肠道的消化过程,发酵产物对动物肠道健康的调控机制,以及饲粮纤维在单胃动物中的应用现状,以期为重新审视纤维结构及其转运机制、探索饲粮纤维的潜在营养价值和制定单胃动物纤维营养的应用措施提供参考。

关键词：饲粮纤维微生物群纤维结构短链脂肪酸代谢机制

Apple SINA11-JAZ2 module is involved in jasmonate signaling response

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2024年第7期66卷 1270-1273页

作者： Di Ai Lei Zhao Chun-Xiang You Yuepeng Han Jian-ping An State Key Laboratory of Plant Diversity and Specialty Crops CAS Key Laboratory of Plant Germplasm Enhancement and Specialty AgricultureWuhan Botanical Garden of Chinese Academy of SciencesWuhan 430074China Hubei Hongshan Laboratory Wuhan 430070China University of Chinese Academy of Sciences Beijing 100049China Apple Technology Innovation Center of Shandong Province College of Horticulture Science and EngineeringShandong Agricultural UniversityTai-An 271018China

Jasmonic acid(JA)is an important phytolipid hormone involved in regulating many processes of plant growth and development,and plays a key role in plant resistance to biotic and abiotic *** the first discovery of methy... 详细信息

关键词： nat Apple involv