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The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability

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Protein & Cell 2011年第9期2卷 755-763页

作者： Kentaro Ito Akinori Takahashi Masahiro Morita Toru Suzuki tadashi yamamoto Division of Oncology Department of Cancer BiologyInstitute of Medical ScienceUniversity of TokyoTokyo 108-8639Japan Cell Signal Unit Okinawa Institute of Science and Technology1919-1Okinawa 904-0412Japan

The human CCR4-NOT deadenylase complex consists of at least nine enzymatic and non-enzymatic *** evidence suggests that the non-enzymatic subunits are involved in the regulation of mRNA deadenylation,although their precise roles remain to be *** this study,we addressed the function of the CNOT1 subunit by depleting its expression in HeLa *** cytometric analysis revealed that the sub G1 fraction was increased in CNOT1-depleted ***,the same level of the sub G1 fraction was seen when cells were treated with a mixture of siRNAs targeted against all enzymatic subunits,suggesting that CNOT1 depletion induces apoptosis by destroying the CCR4-NOT-associated deadenylase *** analysis revealed that CNOT1 depletion leads to a reduction in the amount of other CCR4-NOT ***,the specific activity of the CNOT6L immunoprecipitates-associated deadenylase from CNOT1-depleted cells was less than that from control *** formation of P-bodies,where mRNA decay is reported to take place,was largely suppressed in CNOT1-depleted ***,CNOT1 has an important role in exhibiting enzymatic activity of the CCR4-NOT complex,and thus is critical in control of mRNA deadenylation and mRNA *** further showed that CNOT1 depletion enhanced CHOP mRNA levels and activated caspase-4,which is associated with endoplasmic reticulum ER stress-induced *** together,CNOT1 depletion structurally and functionally deteriorates the CCR4-NOTcomplex and induces stabilization of mRNAs,which results in the increment of translation causing ER stress-mediated *** conclude that CNOT1 contributes to cell viability by securing the activity of the CCR4-NOT deadenylase.

关键词： deadenylation CCR4-NOT small interfering RNA P-bodies apoptosis

Time course of expression of intermediate filament protein vimentin,nestin and desmin in rat renal glomerular injury

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Chinese Medical Journal 2007年第13期120卷 1203-1205页

作者： ZOU Jun CHANG Tian-hui CHANG He Eishin Yaoita Yutaka Yoshida Masaaki Nameta tadashi yamamoto JIN Xin Department of Pharmacy School of Medical Sciences Xiamen University Xiamen 361005 China Department of Clinical Pharmacology School of Pharmaceutical Sciences China Medical University Shengyang 110001 China Division of Cardiology Niigata University Graduate School of Medical and Dental Sciences 1-757 Asahimachi-dori Niigata 951-8510 Japan Department of Structural Pathology Institute of NephrologyNiigata University Graduate School of Medical and Dental Sciences 1-757 Asahimachi-dori Niigata 951-8510 Japan

Podocytes in renal glomemlus express unusual intermediate filament proteins （IFs） for visceral epithelial cells. IFs cytoskeleton is mainly composed of vimentin, nestin and desmin. Tissue injury is often accompanied by changes in gene expression of IFs. Enhanced desmin staining in variety of rat experimental including 2 4 puromycin nephrosis. It has not podocytes are observed in a models of podocyte injury aminonucleoside （PAN） been elucidated whether expression of vimentin and nestin is up-regulated in podocyte injury. To further gain insight into expression of IFs in podocytes, we investigated the time course of vimentin, nestin and desmin in PAN nephrosis.

关键词： intermediate filament vimentin nestin desmin kidney

Enhanced aquaporin 8 expression after subtotal colectomy in rat

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Open Journal of Gastroenterology 2013年第5期3卷 253-258页

作者： Masato Nakano Yu Koyama Hitoshi Nogami tadashi yamamoto Toshifumi Wakai Division of Digestive & General Surgery Niigata University Graduate School of Medical & Dental Sciences Niigata Japan Division of Renal Pathology Niigata University Graduate School of Medical & Dental Sciences Niigata Japan

Background: Aquaporins (AQPs), the family of water-selective channels, are localized in various organs and tissues, including the gastrointestinal (GI) tract. However, the roles of AQPs in the GI tract remain unclear. Materials and Methods: Male SD rats were subjected to subtotal colectomy (Group C, n = 22) or a sham operation (Group S, n = 16) and were sacri-ficed on postoperative days 7, 14, and 28. Total RNAs from the distal ileum and rectum were extracted. Quantitative RT-PCR was performed to measure AQP8 mRNA expression. For light-microscopy or immunohistochemistry, paraffin-embedded sections of 4 μm were prepared with H-E staining or anti-AQP8 antibody reaction. Mann-Whitney U-test was performed to compare the AQP8 distributions between the two groups, and the statistical significance was defined as

关键词： Aquaporin 8 Subtotal Colectomy mRNA Immunohistochemistry

Present Status of EUV Interferometer Development at the Research Center for Soft X-ray Microscopy

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光学精密工程 2001年第5期9卷 405-410页

作者： Masaki yamamoto, tadashi Hatano, Minaji Furudate (Research Center for Soft X-ray Microscopy, Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Japan) Tohoku University 日本

A new interferometer for extreme ultraviolet (EUV) radiation with a laser produced plasma (LPP) laboratory source is under construction. The LPP source is operated with a Sn solid rod target on which pulsed YAG laser is focused to produce high temperature plasma emitting EUV radiation. The source is equipped with a newly designed debris stopper protecting a condenser multilayer mirror from the particle debris of the target. The condenser mirror focuses the light onto an EUV beam-splitter to form transmitted and reflected paths for producing interference fringes of a sharing type. The optical configuration is of a common path based on a triangular path type with a focusing at the beam-splitter, which is enabled to produce fringes by a low coherence radiation with a standard optical quality beam-splitter. The fringes are recorded by an imaging plate with pixels as small as 25μm. The dynamic range of linearity in detection of the EUV light was found to be more than 10 4 with sensitivity of 10 4 photons/pixel, enough for the purpose of interferogram recording, possibly with one laser shot.

关键词： soft X-ray interferometers multilayer mirrors figure error imaging optics

Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity

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Crystal structures of human BTG2 and mouse TIS21 involved in...

Structural basis for inhibition of the Tob-CNOT7 interaction by a fragment screening approach

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Masahiro Morita Toru Suzuki Mark Bartlam tadashi yamamoto Division of Oncology Institute of Medical ScienceUniversity of Tokyo College of Life Sciences & Tianjin State Laboratory of Protein Sciences Nankai University

＜正＞BTG2 is the prototypical member of the TOB family and is known to be involved in cell growth,differentiation,and DNA *** a transcriptional co-regulator,BTG2 interacts

关键词： BTG2 TOB family CAF1 binding deadenylase activity crystal structure

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Protein & Cell 2015年第12期6卷 924-928页

作者： Yuwei Bai (1) (2) Shinya Tashiro (3) Satoru Nagatoishi (3) Toru Suzuki (4) Dongke Yan (1) (2) Ruihua Liu (1) (2) Kouhei Tsumoto (3) Mark Bartlam (1) (2) tadashi yamamoto (4) 1.State Key Laboratory of Medicinal Chemical Biology Nankai University Tianjin 300071 China 2.College of Life Sciences Nankai University Tianjin 300071 China 3.Medical Proteomics Laboratory Institute of Medical Science The University of Tokyo 4-6-1 Shirokanedai Minato-ku Tokyo 108–8639 Japan 4.Cell Signal Unit Okinawa Institute of Science and Technology Graduate University 1919-1 Onna-son Kunigami Okinawa 904-0412 Japan

The anti-proliferative protein Tob belongs to the Tob/BTG family （Matsuda et al., 2001） and plays important roles in cell prolif- eration, embryonic development, cellular differentiation, cancer suppression, and apo... 详细信息

关键词： Surface Plasmon Resonance Surface Plasmon Resonance Response K63A Mutant Tumor Escape Mechanism Mutation W93A

Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity

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生物物理学报 2009年第S1期25卷 369-369页

作者： Masahiro Morita Toru Suzuki Mark Bartlam tadashi yamamoto Division of Oncology Institute of Medical Science University of TokyoMinato-ku Tokyo 108-8639 Japan College of Life Sciences & Tianjin State Laboratory of Protein Sciences Nankai University Tianjin 300071 5 Shenzhen College of International Education

BTG2 is the prototypical member of the TOB family and is known to be involved in cell growth, differentiation, and DNA repair. As a transcriptional co-regulator, BTG2

关键词： BTG2 TOB family CAF1 binding deadenylase activity crystal structure

Aquaporin 8 mRNA expression after intestinal resection in rat

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Open Journal of Gastroenterology 2014年第2期4卷 62-68页

作者： Yu Koyama Hitoshi Kameyama Jun Sakata Takashi Kobayashi Masahiro Minagawa Shin-ichi Kosugi tadashi yamamoto Kohei Akazawa Toshifumi Wakai Department of Medical Informatics Niigata University Graduate School of Medical & Dental Sciences Niigata Japan Department of Renal Pathology Institute of Nephrology Niigata University Graduate School of Medical & Dental Sciences Niigata Japan Division of Digestive & General Surgery Niigata University Graduate School of Medical & Dental Sciences Niigata Japan

Backgrounds: Aquaporins (AQPs), the mammalian water channels, have been localized in various organs, including the gastrointestinal (GI) tract. We examined AQPs expression in rat models of massive intestineal resection to determine the functions of AQPs in the GI tract. Methods: Female Sprague-Dawley rats (n = 15) underwent 90% resection of the small intestine, and Female Wistar-Kyoto rats (n = 10), received subtotal colectomy, and were sacrificed following the operations. RNase protection assay and quantitative reverse transcription-polymerase chain reaction (RT-PCR) were performed to measure the AQPs mRNA expression in the GI tract. Immunohistochemistry was performed to confirm AQP8 protein expression. Results: AQP8 mRNA expression (mean ± standard error), was enhanced in the jejunum of the short bowel rats at days 7 and 14 (37.6% ± 1.4% and 18.5% ± 2.4%, respectively, p < 0.01). Enhancement of AQP8 mRNA was also observed in the remnant rectum of the subtotal colectomized rats at both days 21 and 42 (116.1% ± 4.5% and 143.3% ± 7.4%, respectively, p < 0.01). Immunohistochemistry demonstrated enhanced AQP8 expression in the remnant rectum of the subtotal colectomized rats. No intensive change was observed with other AQPs in both models. Conclusions: Our results suggest a compensatory role of AQP8 in the maintenance of intestinal fluid balance.

关键词： Aquaporin 8 Short Bowel Subtotal Colectomy RNase Protection Assay QuantitativeRT-PCR

The Protocadherin 17 Gene Affects Cognition, Personality, Amygdala Structure and Function, Synapse Development and Risk of Major Mood Disorders

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The Protocadherin 17 Gene Affects Cognition, Personality, Am...