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Selective Deactivation of Gibberellins below the Shoot Apex is Critical to Flowering but Not to Stem Elongation of Lolium

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Molecular Plant 2008年第2期1卷 295-307页

作者： Rod W. King Lewis N. Mander t.rben asp Colleen R MacMillan Cheryl A. Blundell Lloyd t. Evans CSIRO Plant Industry Canberra ACT 2600 Australia Research School of Chemistry Australian National University Canberra ACT 0200 Australia Department of Genetics and-Biotechnology University of Aarhus 4200 Slagelse Denmark Present Address: ENSIS PO Box E 4008 Kingston ACT 2604 Australia

Gibberellins （GAs） cause dramatic increases in plant height and a genetic block in the synthesis of GA1 explains the dwarfing of Mendel＇s pea. For flowering, it is GAs which is important in the long-day （LD） responsive grass, Loliurn. As we show here, GA1 and GA4 are restricted in their effectiveness for flowering because they are deactivated by C-2 hydroxylation below the shoot apex. In contrast, GAs is effective because of its structural protection at C-2. Excised vegetative shoot tips rapidly degrade [14C]GA1, [14C]GA4, and [14C]GA20 （〉80% in 6 h）, but not [14C]GAs. CoincidentaUy, genes encoding two 2β-oxidases and a putative 16-17-epoxidase were most expressed just below the shoot apex （〈3 mm）. Further down the immature stem （〉4 mm）, expression of these GA deactivation genes is reduced, so allowing GA1 and GA4 to promote sub-apical stem elongation. Subsequently, GA degradation declines in florally induced shoot tips and these GAs can become active for floral development. Structural changes which stabilize GA4 confirm the link between florigenicity and restricted GA 2β-hydroxylation （e.g. 2α-hydroxylation and C-2 di-methylation）. Additionally, a 2-oxidase inhibitor （trinexapac Ethyl） enhanced the activity of applied GA4, as did limiting C-16,17 epoxidation in 16,17-dihydro GAs or after C-13 hydroxylation. Overall, deactivation of GA1 and GA4 just below the shoot apex effectively restricts their florigenicity in Loliurn and, conversely, with GAs, C-2 and C-13 protection against deactivation allows its high florigenicity. Speculatively, such differences in GA access to the shoot apex of grasses may be important for separating floral induction from inflorescence emergence and thus could influence their survival under conditions of herbivore predation.

关键词： flowering shoot apices Shoots Gallium Lolium plant height Deactivation G(A1) ganglioside Apex APEX1 protein, human Gibberellins stem elongation

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Clear Image Auido t4电源线路隔离器——排除噪音对音乐的干扰

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中国电子商情(基础电子) 1997年第4期 67-69页

作者： asp 张玲俐

可怜的斯各特!被音响评论员如此刁难,日子一定难过极了。电话铃响。我拿起话筒道:"喂——" "喂——我是斯各特。我想让你来评论一台机子"。 "啊哈——是前级还是功放"? "都不是,是电源线路调节器"。 "唔,好吧,斯各特,只是我并不热衷这东... 详细信息

可怜的斯各特!被音响评论员如此刁难,日子一定难过极了。电话铃响。我拿起话筒道:"喂——" "喂——我是斯各特。我想让你来评论一台机子"。 "啊哈——是前级还是功放"? "都不是,是电源线路调节器"。 "唔,好吧,斯各特,只是我并不热衷这东西,它的质量算不上特别好,尽管能降低噪音,不过连续使用一段时间后,常常使人抓不住音乐的头绪,到后来我只有把它扔在一边。你应该找那种能欣赏它的人,比如说HP(tAS杂志总编,编者注)他说不定会……"

关键词：噪音噪声语音电源线路 Clear Image Auido t4 立体声交流电电流单声道唱片音响效果舞台效果数模转换器电工仪表变换器隔离器单向器音乐

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Ethyl acetate extract of Smilax glabra Roxb roots and its major active compound astilbin promote osteoblastogenesis in vitro by upregulating bone cell differentiation-associated genes

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Asian Pacific Journal of tropical Biomedicine 2021年第12期11卷 553-560页

作者： Huyen t.t.Nguyen Minh t.H.Nguyen thu X.Nguyen Quan M.Pham Ha X.Nguyen Phuong t.M.Nguyen University of Science and technology of Hanoi Vietnam Academy of Science and TechnologyHanoiVietnam Institute of Biotechnology Vietnam Academy of Science and TechnologyHanoiVietnam Institute of Natural Products Chemistry Vietnam Academy of Science and TechnologyHanoiVietnam Graduate University of Science and technology Vietnam Academy of Science and TechnologyHanoiVietnam

Objective:to investigate the osteoblastogenic activity of the ethyl acetate(EtOAc)extract of Smilax glabra Roxb roots and its major active compound ***:Astilbin was isolated from EtOAc extract using silica gel chromatography combined with fraction *** structure of astilbin was determined by analysis of the spectroscopic data in comparison with the *** method was used to detect the *** phosphatase(ALP)activity was determined by the spectrophotometric method at 405 nm using p-nitrophenyl phosphate as a *** deposition was stained with alizarin red-S,distained with cetylpyridium chloride,and quantified at 562 *** silico model for astilbin-ALP interaction was analyzed using AutoDock *** changes in expression of osteoblast differentiation related genes were determined using quantitative real-time ***:Both the EtOAc extract and astilbin had no toxicity toward osteoblast MC3t3-E1 cells at 5.0,10,25,and 50μg/*** 25μg/mL,they enhanced ALP activity and mineralization of osteoblasts up to 30%and 55%for the EtOAc extract and 22%and 41%for astilbin,*** docking analysis of astilbin-ALP interaction revealed Arg167,asp320,His324,and His437 were key residues participating in hydrophobic interaction;meanwhile,His434 and thr436 residues were involved in hydrogen bond formation in the active site of human tissue-nonspecific ***,the expression level of genes opn,col1,osx,and runx2 were up-regulated in astilbin treated samples with the fold changes as 2.2;3.7;4.1;2.3,respectively at 10μg/mL(Pthe EtOAc extract and its major compound astilbin exhibit osteoblastogenic activity by up-regulating important markers for bone cell *** could be a new and promising osteogenic agent with dual actions for therapeutic applications.

关键词： Smilax glabra Roxb Astilbin Cytotoxicity Osteogenesis Osteoblast Ethyl acetate extract

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土耳其电信体育场

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城市建筑 2010年第11期7卷 91-94页

作者：无钱辰伟（译） asp建筑师事务所不详

作为土耳其足球豪门之一的新主场．这座全新的土耳其电信体育场将在伊斯坦布尔市中心西北几公里处落户。该体育场将满足国际足联和欧足联对承办欧洲足球冠军联赛或世界足球锦标赛决赛阶段比赛场地的各项要求。体育场基址位于伊斯坦布尔... 详细信息

作为土耳其足球豪门之一的新主场．这座全新的土耳其电信体育场将在伊斯坦布尔市中心西北几公里处落户。该体育场将满足国际足联和欧足联对承办欧洲足球冠军联赛或世界足球锦标赛决赛阶段比赛场地的各项要求。体育场基址位于伊斯坦布尔市中心西北方向．毗邻连接安卡拉与埃迪尔内的泛欧高速公路（trans—European—Motorway）的一座小山顶上．沿东北至西南向的轴线展开。

关键词：体育场土耳其电信伊斯坦布尔比赛场地高速公路市中心安卡拉

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Muscle amino acid profiles of eleven species of aquacultured animals and their potential value in feed formulation

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Aquaculture and Fisheries 2024年第4期9卷 642-652页

作者： Ewen McLean Kelly B.Alfrey Delbert M.Gatlin III t.Gibson Gaylord Frederick t.Barrows Aqua Cognoscenti LLC 479 Henslowe LaneWest ColumbiaSC29170USA Anthropocene Institute 855 El Camino RealSte 13A N399Palo AltoCA94301USA texas A&M University Department of Ecology and Conservation BiologyCollege StationTX77843USA US Fish and Wildlife Service Bozeman Fish Technology Center4050 Bridger Canyon RoadBozemanMT59715USA Aquatic Feed technologies LLC 48 West Plaza del LagoIslamoradaFL33036USA

the complete quantitative essential amino acid(EAA)requirements of aquacultured animals are largely unknown except for a handful of *** is problematic because formulation of least-cost diets for target animals demands precise knowledge of EAA *** way of approximating EAA requirements is to use correlations between whole-body and/or muscle EAA profiles using essential to total EAA ratios(A/E ratios).this method requires a reference,quantified EAA requirement,usually lysine(Lys).to systematically evaluate the A/E ratio method,muscle AA profiles were measured in 10 species of teleost and whiteleg *** fish,Lys represented the dominant muscle EAA measured,ranging between 7.06 and 9.58 g/100 g protein,whereas,in shrimp,arginine(Arg)was quantified as the principal *** non-EAA,glutamate(Glu)and glutamine were consistently the NEAA recorded at highest levels whereas,in shrimp,Glu+Gln was highest followed by aspartate(asp)plus asparagine(Asn).Except for Arg,which was twice that of fish(Pteleg shrimp expressed similar muscle A/E *** of relationship between muscle EAA levels and known requirements were all strongly and positively correlated for non-salmonid *** using Lys as the reference EAA there were few semblances between measured and estimated EAA requirements.

关键词： Aquafeed A/E ratio EAA requirement Ideal protein Muscle Fishmeal replacement

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Homogenization in clay barriers and seals:two case studies

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Journal of Rock Mechanics and Geotechnical Engineering 2013年第3期5卷 191-199页

作者： A.Gens B.Valleján M.t.Zandarín M.Sánchez Department of Geotechnical Engineering and Geosciences Universitat Politècnica de Catalunya Zachry Department of Civil Engineering Texas A&M UniversityCollege Station

the paper presents two case studies that provide information on the process of homogenization of initially heterogeneous clay barriers and seals. the first case is the canister retrieval test performed in the asp0 Hard Rock Laboratory （Sweden）. the heterogeneity arises from the use of a combination of blocks and pellets to construct the engineered barrier. the degree of homogenization achieved by the end of the tests is evaluated from data obtained during the dismantling of the test. to assist in the interpretation of the test, a fully coupled thermo-hydro-mechanical （tHM） analysis has been carried out. the second case involves the shaft sealing test performed in the HADES underground research laboratory （URL） in Mol （Belgium）. Here the seal is made up of a heterogeneous mixture of bentonite pellets and bentonite powders. In addition to the full scale test, the process of homogenization of the mixture has also been observed in the laboratory using X-ray tomography. Both field test and laboratory tests are successfully modelled by a coupled hydro-mechanical （HM） analysis using a double structure constitutive law. the paper concludes with some considerations on the capability of highly expansive materials to provide a significant degree of homogenization upon hydration.

关键词： Nuclear waste disposal Clay barriers Clay seals Heterogeneity Compacted soils Coupled analyses Unsaturated soils

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Quantum Simulation of 2p-πElectronic Hamiltonian in Molecular Ethylene by Using an NMR Quantum Computer

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Journal of Quantum Information Science 2013年第2期3卷 78-84页

作者： Deniz türkpence Azmi Gencten Department of Physics Faculty of Arts and SciencesOndokuz Mayis UniversitySamsunTurkey

Classical simulation of a quantum system is a hard problem. It’s known that these problems can be solved efficiently by using quantum computers. this study demonstrates the simulation of the molecular Hamiltonian of 2p-π electrons of ethylene in order to calculate the ground state energy. the ground state energy is estimated by an iterative phase estimation algorithm. the ground state is prepared by the adiabatic state preparation and the implementation of the procedure is carried out by numerical simulation of two-qubit NMR quantum simulator. the readout scheme of the simulator is performed by extracting binary bits via NMR interferometer.

关键词： NMR Quantum Computer Quantum Simulation Molecular Ethylene asp IPEA

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Evaluation of Antioxidants, total Phenolics and Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

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Journal of Renewable Materials 2019年第7期7卷 667-682页

作者： Bahaa t.Shawky Mohammed Nagah Mosad A.Ghareeb Gamal M.El-Sherbiny Saad A.M.Moghannem Mohamed S.Abdel-Aziz Microbial Chemistry Department National Research CentreDokki-12622CairoEgypt Medicinal Chemistry Department Theodor Bilharz Research InstituteKornaish El-NileWarrak El-HadarImbaba(P.O.30)Giza 12411Egypt Botany and Microbiology Department Faculty of ScienceAzhar UniversityNasr CityCairoEgypt

Rice straw(RS)collected from Egyptian agricultural environment(Al-Masied village-Alsharqia Governorate-Egypt)was used as substrate for three fungal isolates namely:4b,1asp,Pleurotus ostreatus NRRL 3501 and mixed culture of(4b,1asp).Isolates number 4b and 1asp were identified using the molecular technique(18S rRNA).Isolate 4b and 1asp,were identified as trichoderma saturnisporum MN1-EGY and aspergillus niger MN2-EGY,*** saturnisporum MN1-EGY extract exhibited the highest antimicrobial activity compared to the other fungal *** phenolic,antimicrobial and antioxidant activities were also determined from the ethyl acetate extract of rice straw inoculated by these *** antioxidant scavenging concentration(SC50)values of the tested samples were 88.84,91.45,94.65 and 100.0μg/ml,respectively for Pleurotus sp.,aspergiluus niger,aspergiluus niger+trichoderma sp.,and trichoderma sp.,after 15 days incubation,compared to ascorbic acid as positive control with SC50 value equal to 8.0μg/*** phenolic contents(tPCs)were maximum in Pleurotus *** and found to be the most polyphenolic enriched sample(380.64 mg GAE/g dry extract)compared to extracts of aspergiluusniger,aspergiluus niger+trichoderma sp.,and trichoderma sp.(261.89,198.52,and 119.80 mg GAE/g dry extract),*** GC-MS analysis has been also performed for the promising extracts.

关键词： Rice straw fungi molecular identification antimicrobial antioxidant total phenolics

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Visual Interdev 6 Bible

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1998年

作者： Richard t Mansfield Debbie Revette

ISBN: (纸本)9780764531354

From the Publisher: take complete control of Visual InterDev's new team-based RAD development tools with support for the latest Web technologies - including DHtML, design-time controls, asp, themes, Style Sheets, and graphics. Using in-depth tutorials and hands-on examples, Visual InterDev 6 Bible walks you through all the stages of site creation from concept to scripting to deployment. Brimming with new ideas, tips, and shortcuts, this resource brings you all the information you need to build Web sites on the cutting edge of technology.

关键词： Web sites

来源：畅想之星图书光盘版（南... 评论

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3-Dimensional Structure Observation of Mammalian D-aspartyl Endopeptidase by High-Resolution Atomic Force Microscopy

3-Dimensional Structure Observation of Mammalian D-Aspartyl ...

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作者： t.Kinouchi N.Fujii Research Reactor Institute Kyoto University

INtRODUCtION It is suggested that D-isomers of aspartic acid (D-asp) containing protein are spontaneously generated by oxidative stress and would cause many aging -related protein-misfolding diseases such as Alzheimer’s *** have identified a D-asp-containing protein-specific protease, D-aspartyl endopeptidase(DAEP) from mammalian mitochondria,therefore DAEP would serve as a defensive system against the noxious D-asp-containing

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