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Heterogeneously elevated branched-chain/aromatic amino acids among new-onset type-2 diabetes mellitus patients are potentially skewed diabetes predictors

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World Journal of Diabetes 2024年第1期15卷 53-71页

作者： Min Wang Yang Ou Xiang-Lian Yuan Xiu-Fang Zhu Ben Niu Zhuang Kang Bing Zhang Anwar Ahmed Guo-Qiang Xing Heng su School of Chemical Science and technology Yunnan UniversityKunming 650091Yunnan ProvinceChina Department of Endocrinology The First People’s Hospital of Yunnan ProvinceKunming 650032Yunnan ProvinceChina Clinical Laboratory Nanchong Central Hospital&The Second Clinical Medical College of North Sichuan Medical UniversityNanchong 637000Sichuan ProvinceChina Department of Preventive Medicine and Biostatistics Uniformed Services University of the Health SciencesBethesdaMD 20814United States the Affiliated Hospital and Second Clinical Medical College North Sichuan Medical UniversityNanchong 637000Sichuan ProvinceChina Department of Research and Development Lotus *** LLCGaithersburgMD 20878United States

BACKGROUND the lack of specific predictors for type-2 diabetes mellitus(t2DM)severely impacts early intervention/prevention *** branched-chain amino acids(BCAAs:Isoleucine,leucine,valine)and aromatic amino acids(AAAs:tyrosine,tryptophan,phenylalanine)show high sensitivity and specificity in predict.ng diabetes in animals and predict t2DM 10-19 years before t2DM onset in clinical ***,improvement is needed to support its clinical *** to evaluate the effects of body mass index(BMI)and sex on BCAAs/AAAs in new-onset t2DM individuals with varying body *** Ninety-seven new-onset t2DM patients(ng in BMI[normal weight(NW),n=33,BMI=22.23±1.60;overweight,n=42,BMI=25.9±1.07;obesity(OB),n=22,BMI=31.23±2.31]from the First People’s Hospital of Yunnan Province,Kunming,China,were ***-way and 2-way ANOVAs were conducted to determine the effects of BMI and sex on BCAAs/*** Fast.ng serum AAAs,BCAAs,glutamate,and alanine were greater and high-density lipoprotein(HDL)was lower(Pt2DM patients than in NW-t2DM patients,especially in male OB-t2DM ***,histidine,leucine,methionine,and lysine were greater in male patients than in female ***,histidine,alanine,glutamate,lysine,valine,methionine,leucine,isoleucine,tyrosine,phenylalanine,and tryptophan were significantly correlated with abdominal adiposity,body weight and BMI,whereas isoleucine,leucine and phenylalanine were negatively correlated with *** Heterogeneously elevated amino acids,especially BCAAs/AAAs,across new-onset t2DM patients in differing BMI categories revealed a potentially skewed prediction of t2DM *** higher BCAA/AAA levels in obese t2DM patients would support t2DM prediction in obese individuals,whereas the lower levels of BCAAs/AAAs in NW-t2DM individuals may underestimate t2DM risk in NW *** potentially skewed t2DM prediction should be considered when BCAAs/AAAs are to

关键词： Hyperaminoacidemia Branched-chain/aromatic amino acids New-onset type-2 diabetes Predictor Obesity Sex

Study on Characteristics of a High-Precision Cold Gas Micro thruster

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Engineering（科研） 2024年第1期16卷 38-45页

作者： Zhaoli Wang Changbin Guan Xudong Wang Weijie Zheng Longfei su Advanced Space Propulsion Lab Beijing Institute of Control Engineering Beijing China

In order to improve the reliability of the spacecraft micro cold gas propulsion system and realize the precise control of the spacecraft attitude and orbit, a micro-thrust, high-precision cold gas thruster is carried out, at the same time due to the design requirements of the spacecraft, this micro-thrust should be continuous working more than 60 minutes, the traditional solenoid valve used for the thrusts can’t complete the mission, so a long-life micro latching valve is developed as the control valve for this micro thruster, because the micro latching valve can keep its position when it cuts off the outage. Firstly, the authors introduced the design scheme and idea of the thruster. Secondly, the performance of the latching valve and the flow characteristics of the nozzle were simulated. Finally, from the experimental results and compared with the numerical study, it shows that the long-life micro cold gas thruster developed in this paper meets the mission requirements.

关键词： High-Precision Micro thruster Performance Flow Characteristic Experiment

Cyclic Dinucleotide Self-Assembled Nanoparticles as a Carrier-Free Delivery Platform for St.ng-Mediated Cancer Immunotherapy

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CCS Chemistry 2024年第1期6卷 177-195页

作者： Lang Zhao Shao-Hua Zhuo tian-Yang Wang Jun-Jun Wu Jing-Yun su Wen-Hao Li Bo-Dou Zhang Yan-Mei Li Key Lab of Bioorganic Phosphorus Chemistry&Chemical Biology Department of ChemistryTsinghua UniversityBeijing 100084 Beijing Institute for Brain Disorders Beijing 100069 Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084

Cyclic dinucleotides(CDNs)are natural agonists of the stimulator of interferon genes(St.ng),which is an attractive immunotherapy ***,CDNs and their derivatives are being investigated ***,the poor bioavailability of exogenous CDNs has limited their application in *** nanocarriers are widely used for cytosolic delivery of CDNs,their loading capacity is insufficient,and their complicated composition and purification process raises bio-compatibility ***,we report a super-simplified CDN self-assembly strategy for carrier-free delivery of *** the presence of excess K^(+),CDNs form oligomers which further self-assemble with divalent metal ions(such as Mn^(2+))to form nanoparticles(NPs)in aqueous *** demonstrate that the self-assembled CDN NPs promote cellular uptake of CDNs and enhance tumor immunogenicity by remodeling the tumor microenvironment,inducing immunogenic tumor cell death and increasing tumorinfiltrat.ng lymphocytes,which is conducive to the generation of tumor neoantigen-specific t-cell *** also demonstrate that the use of CDN NPs alone or in combination with immune checkpoint blockades inhibits tumor growth,highlight.ng the fact that CDN NPs are a potent platform for cancer immunotherapy.

关键词： cyclic dinucleotide St.ng carrierfree delivery tumor immunogenicity cancer immunotherapy

N6-methyladenosine methylation regulates the tumor microenvironment of Epstein-Barr virus-associated gastric cancer

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2555-2570页

作者： Yu Zhang Fang Zhou Ming-Yu Zhang Li-Na Feng Jia-Lun Guan Ruo-Nan Dong Yu-Jie Huang su-Hong Xia Jia-Zhi Liao Kai Zhao Department of Gastroenterology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Institute of Liver and Gastrointestinal Diseases Hubei Key Laboratory of Hepato-Pancreato-Biliary DiseasesTongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina Department of Pharmacy Wuhan Fourth HospitalWuhan 430030Hubei ProvinceChina

BACKGROUND N6-methyladenosine(m6A)methylation modification exists in Epstein-Barr virus(EBV)primary infection,latency,and lytic *** also modifies EBV latent genes and lytic ***-associated gastric cancer(EBVaGC)is a distinctive molecular subtype of *** hypothesized EBV and m6A methylation regulators interact with each other in EBVaGC to differentiate it from other types of *** to investigate the mechanisms of m6A methylation regulators in EBVaGC to determine the differentiat.ng factors from other types of *** First,the Cancer Gene Atlas and Gene Expression Omnibus databases were used to analyze the expression pattern of m6A methylation regulators between EBVaGC and EBV-negative GC(EBVngC).Second,we identified Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment of m6A-related differentially expressed *** quantified the relative abundance of immune cells and inflammatory factors in the tumor microenvironment(tME).Finally,cell count.ng kit-8 cell proliferation test,transwell test,and flow cytometry were used to verify the effect of insulin-like growth factor binding protein 1(IGFBP1)in EBVaGC cell *** m6A methylation regulators were involved in the occurrence and development of *** with EBVngC,the expression levels of m6A methylation regulators Wilms tumor 1-associated protein,RNA binding motif protein 15B,CBL proto-oncogene like 1,leucine rich pentatricopeptide repeat containing,heterogeneous nuclear ribonucleoprotein A2B1,IGFBP1,and insulin-like growth factor 2 binding protein 1 were significantly downregulated in EBVaGC(Pthe overall survival rate of EBVaGC patients with a lower expression level of IGFBP1 was significantly higher(P=0.046).GO and KEGG functional enrichment analyses showed that the immunity pathways were significantly activated and rich in immune cell infiltration in *** with EBVngC,the infiltration of activated CD4+t cells,activated CD8+t cel

关键词： N6-methyladenosine methylation tumor microenvironment Epstein-Barr virus Gastric cancer Insulin-like growth factor binding protein 1

Evaluation of in situ spoilage ability of common facultative anaerobic bacteria and confirmation of volatile spoilage markers in vacuum-packaged lamb

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Food Science and Human Wellness 2024年

作者： Xiangyuan Wen Dequan Zhang su Wang Zhiyuan Xiao Xin Li Debao Wang Wei Yang Chengjun Min Chengli Hou Institute of Food Science and technology， Chinese Academy of Agricultural Sciences， Key Laboratory of Agro-Products Quality and Safety Control in Storage and transport Process， Ministry of Agriculture and Rural Affairs Institute of Agricultural Product Processing and Nutritional Health， Chinese Academy of Agricultural Sciences (Cangzhou) sunrise Material Co.， Ltd Jiangyin Beijing Ershang Meat Food Group Co.， Ltd

Microbial growth causes lamb spoilage. this study explored the spoilage ability of Latilactobacillus sakei (L. sakei)， Serratia proteamaculans (S. proteamaculans) and Hafnia proteus (H. proteus) in vacuum-packed raw lamb， including growth ability， degradation of protein and lipid， and change of volatile organic compounds (VOCs) profile， meanwhile screened the key VOCs produced by the targeted strains with meat background excluding， finally confirmed the volatile spoilage marker of vacuum-packaged lamb by comparing with our previous work. the results showed that L. sakei， S. proteamaculans and H. proteus had excellent growth ability. L. sakei inoculated group significantly reduced the pH value， showed higher tCA-soluble peptides content， and excellently degraded sarcoplasmic and myofibrillar proteins. About free amino acids， L. sakei significantly degraded serine， arginine and aspartic acid， while S. proteamaculans and H. proteus significantly degraded serine and lysine. In addition， L. sakei had a strongest effect on promot.ng free fatty acids production， followed by S. proteamaculans and finally H. proteus. Evaluat.ng from various indicators， the co-culture of the three strains did not have any effect. the key volatiles produced by L. sakei were 1-hexanol， acetic acid and hexanoic aicd， S. proteamaculans were 1-hexanol and acetoin， and H. proteus was 1-hexanol， acetic acid and acetoin. In the end， 1-hexanol， hexanoic acid and acetoin were proven to be spoilage markers for vacuum-packaged and chilled lamb. this study can provide fundamental information for inhibit.ng and rapid identification of spoilage in vacuum-packaged lamb.

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青藏高原及其周边古近纪综合地层、生物群与古地理演化

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中国科学：地球科学 2024年第4期54卷 1308-1342页

作者：刘佳宋艾丁林苏涛周浙昆中国科学院西双版纳热带植物园热带森林生态学重点实验室勐腊666303 中国科学院南京地质古生物研究所现代古生物学和地层学国家重点实验室南京210008 云南大学古生物研究院昆明650500 中国科学院青藏高原研究所地球系统科学与资源环境重点实验室北京100101

古近纪是陆地和海洋生态系统劫后复苏并逐渐接近其现代面貌的关键时期.在青藏高原及其周边区域,古近纪也是青藏高原-喜马拉雅造山带构造活动、亚洲气候重组、生物多样性演化的重要时期.限于研究条件、认识程度等的制约,青藏高原及其周... 详细信息

古近纪是陆地和海洋生态系统劫后复苏并逐渐接近其现代面貌的关键时期.在青藏高原及其周边区域,古近纪也是青藏高原-喜马拉雅造山带构造活动、亚洲气候重组、生物多样性演化的重要时期.限于研究条件、认识程度等的制约,青藏高原及其周边地区在地层划分方面还存在诸多争议.近年来,该区域开展了大量的沉积岩石学、磁性地层学、同位素年代学等工作,发现和报道了大量各种门类的化石,更系统地深化了生物地层学研究,为梳理青藏高原及其周边古近纪综合地层、生物群和古地理演化打下了坚实的基础.本文基于国际古近系划分和对比标准,以岩石地层和生物地层为基础,综合近年来同位素年代学、磁性地层学和地球化学等研究工作,在前人工作基础上梳理青藏高原及其周边不同构造单元的地层划分与对比框架.第二次青藏高原综合科学考察开展以来,古近纪植物群相关研究逐渐丰富.依据这些植物群最新的年代结果,本文讨论了灭绝类群和新出现类群的生物地层意义.对“桉属”、棕榈科等化石类群的新认识将古近纪青藏地区植物区系与冈瓦纳大陆的大洋洲、南美洲生物区系建立联系.雅鲁藏布江缝合带两侧植物群重要类群的演化历史也指示印度板块和欧亚大陆的碰撞时间在约65~54Ma.植物群的古高程重建结果表明,横断山区在早渐新世之前就已经形成现今的地貌格局;古近纪青藏地区主要缝合带附近的温暖湿润低地是动植物类群交流的主要通道;古近纪低矮的喜马拉雅山脉不能对南来的印度洋水汽形成有效的阻隔作用.

关键词：青藏高原化石生物地层古环境古地理古近纪

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Boost.ng the catalytic activity toward oxygen reduction via a heterostructure of porous iron oxide-decorated 2D NiO/ng nanosheets

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Journal of Energy Chemistry 2024年第6期93卷 669-681,I0016页

作者： Kakali Maiti Matthew t.Curnan Hyung Jun Kim Kyeounghak Kim Jeong Woo Han Department of Chemical Engineering Pohang University of Science and Technology(POSTECH)PohangGyeongbuk 37673Republic of Korea Department of Materials Science and Engineering Research Institute of Advanced MaterialsSeoul National UniversitySeoul 08826Republic of Korea Department of Chemical Engineering Clean-Energy Research InstituteHanyang UniversitySeoul 04763Republic of Korea CSIR-Institute of Minerals and Materials technology RRL CampusAcharya ViharBhubaneswarOdisha 751013India

As a noble metal substitute,two-dimensional(2D)hierarchical nano-frame structures have attracted great interest as candidate catalysts due to their remarkable advantages-high intrinsic activity,high electron mobility,and straightforward surface ***,they may replace Pt-based catalysts in oxygen reduction reaction(ORR)***,a simple method is developed to design hierarchical nano-frame structures assembled via 2D NiO and N-doped graphene(ng)*** procedure can yield nanostructures that satisfy the criteria correlated with improved electrocatalytic performance,such as large surface area,numerous undercoordinated atoms,and high defect ***,porous ng nanosheet architectures,featuring NiO nanosheets densely coordinated with accessible holey Fe_(2)O_(3) moieties,can enhance mesoporosity and balance *** improvements can facilitate charge transport and expose formerly inaccessible reaction sites,maximizing active site density *** functional theory(DFt)calculations reveal favored O_(2) adsorption and dissociation on Fe_(2)O_(3) hybrid structures when supported by 2D NiO and ng nanomaterials,given 2D materials donated charge to Fe_(2)O_(3) active *** systematic studies reveal that synergistic contributions are responsible for enriching the catalytic activity of Fe_(2)O_(3)@NiO/ng in alkaline media-encompassing internal voids and pores,unique hierarchical support structures,and concentrated N-dopant and bimetallic atomic ***,this work expands the toolbox for designing and synthesizing highly efficient 2D/2D shelled functional nanomaterials with transition metals,endeavoring to benefit energy conversion and related ORR applications.

关键词： N-doped graphene Holey Fe_(2)O_(3)nanocrystals NiO nanosheets High catalytic performance ORR

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siRNA沉默DJ-1通过PtEN/Akt通路对人胃癌细胞影响机制

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中华肿瘤防治杂志 2024年第13期31卷 807-817页

作者：汤欢夏红刘芳苏坚苏波苏琦南华大学肿瘤研究所湖南省肿瘤细胞与分子病理学重点实验室永州市中心医院肿瘤科南华大学附属第二医院病理科南华大学药学与药理学研究所湖南省高校药物蛋白质组学重点实验室

目的探讨siRNA沉默DJ-1基因体内外对人胃癌MGC803细胞的影响机制。方法构建沉默DJ-1基因MGC803细胞；四甲基偶氮唑蓝(Mtt)比色法、平板克隆、细胞划痕和侵袭实验检测沉默DJ-1基因对MGC803细胞增殖、克隆形成、迁移与侵袭的影响；实时... 详细信息

目的探讨siRNA沉默DJ-1基因体内外对人胃癌MGC803细胞的影响机制。方法构建沉默DJ-1基因MGC803细胞；四甲基偶氮唑蓝(Mtt)比色法、平板克隆、细胞划痕和侵袭实验检测沉默DJ-1基因对MGC803细胞增殖、克隆形成、迁移与侵袭的影响；实时荧光定量PCR(qRt-PCR)和蛋白质印迹法检测DJ-1、PtEN、Akt、p-Akt、Snail、Vimentin、E-cadherin、基质金属蛋白酶9(MMP-9)与基质金属蛋白酶组织抑制因子3(tIMP3)的表达；相差显微镜观察DJ-1沉默对MGC803细胞形态学的影响；裸鼠实验检测DJ-1沉默对MGC803细胞移植瘤的影响。结果分别用4组si-DJ-1及si-NC质粒转染MGC803细胞，其中si-DJ-1A组DJ-1 mRNA与蛋白表达下调较为显著(Ptt法检测结果显示，24、48和72 h后，si-DJ-1组增殖活性分别较MGC803对照组和si-NC组降低，均Ptin mRNA(F=44.379,PtIMP3 mRNA(F=16.480,P=0.004)上调；si-DJ-1组DJ-1(F=6.393,P=0.004)、p-Akt (F=12.980,P=0.007)、Snail(F=381.000,Ptin(F=99.750,PtEN (F=36.880,PtIMP3(F=217.800,Pt=5.273,Ptin和CD-34阳性表达降低，而PtEN和E-cadherin表达增强。结论 DJ-1沉默可通过PtEN/Akt通路体内外抑制MGC803细胞增殖、迁移、侵袭与上皮细胞-间充质转化。

关键词：人胃癌MGC803细胞 DJ-1沉默 PtEN/Akt通路增殖迁移侵袭上皮-间质转化

target.ng a disintegrin and metalloprotease(ADAM)17-CD122 axis enhances CD8^(+)t cell effector differentiation and anti-tumor immunity

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Signal transduction and targeted therapy 2024年第7期9卷 3097-3111页

作者： Lina sun Anjun Jiao Haiyan Liu Renyi Ding Ning Yuan Biao Yang Cangang Zhang Xiaoxuan Jia Gang Wang Yanhong su Dan Zhang Lin Shi Chenming sun Aijun Zhang Lianjun Zhang Baojun Zhang Department of Pathogenic Microbiology and Immunology School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anShaanxi 710061China Institute of Infection and Immunity Translational Medicine InstituteXi’an Jiaotong University Health Science CenterXi’anShaanxi 710061China Key Laboratory of Environment and Genes Related to Diseases Ministry of EducationXi’anShaanxi 710061China Xi’an Key Laboratory of Immune Related Diseases Xi’anShannxi 710061China Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy Cancer InstituteXuzhou Medical UniversityXuzhouJiangsuChina Department of Pediatrics Qilu Hospital of Shandong UniversityJinanChina National Key Laboratory of Immunity and Inflammation Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina Key Laboratory of Synthetic Biology Regulatory Elements Suzhou Institute of Systems MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeSuzhou 215123 JiangsuChina

CD8^(+)t cell immune responses are regulated by multi-layer networks,while the post-translational regulation remains largely *** ectodomain shedding is an important post-translational process orchestrat.ng receptor expression and signal transduction through proteolytic cleavage of membrane ***,by target.ng the sheddase A Disintegrin and Metalloprotease(ADAM)17,we defined a post-translational regulatory mechanism mediated by the ectodomain shedding in CD8^(+)t *** and proteomic analysis revealed the involvement of post-translational regulation in CD8^(+)t cells.t cellspecific deletion of ADAM17 led to a dramatic increase in effector CD8^(+)t cell differentiation and enhanced cytolytic effects to eliminate pathogens and ***,ADAM17 regulated CD8^(+)t cells through cleavage of membrane ***17 inhibition led to elevated CD122 expression and enhanced response to IL-2 and IL-15 stimulation in both mouse and human CD8^(+)t ***,inhibition of ADAM17 in CD8^(+)t cells improved the efficacy of chimeric antigen receptor(CAR)t cells in solid *** findings reveal a critical post-translational regulation in CD8^(+)t cells,providing a potential therapeutic strategy of target.ng ADAM17 for effective anti-tumor immunity.

关键词： immunity stimulation involvement