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Expression and purification of recombinant human serum albumin from selectively terminable transgenic rice

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第10期14卷 867-874页

作者： Qing zhang Hui YU Feng-zhen zhang Zhi-cheng SHEN State Key Laboratory of Rice Biology and Institute of Insect Sciences Zhejiang University

Human serum albumin(HSA) is widely utilized for medical purposes and biochemical *** rice has proved to be an attractive bioreactor for mass production of recombinant HSA(rHSA).However,transgene spread is a major environmental and food safety concern for transgenic rice expressing proteins of medical *** study aimed to develop a selectively terminable transgenic rice line expressing HSA in rice seeds,and a simple process for recovery and purification of rHSA for economical *** HSA expression cassette was inserted into a t-DNA vector encoding an RNA interference(RNAi) cassette suppressing the CYP81A6 *** gene detoxifies the herbicide bentazon and is linked to the 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS) cassette which confers glyphosate *** Sepharose Fast Flow(ANX FF) anion exchange chromatography coupled with Butyl Sepharose High Performance(Butyl HP) hydrophobic interaction chromatography was used to purify rHSA.A transgenic rice line,HSA-84,was obtained with stable expression of rHSA of up to 0.72% of the total dry weight of the dehusked rice *** line also demonstrated high sensitivity to bentazon,and thus could be killed selectively by a spray of bentazon.A two-step chromatography purification scheme was established to purify the rHSA from rice seeds to a purity of 99% with a recovery of 62.4%.Results from mass spectrometry and N-terminus sequencing suggested that the purified rHSA was identical to natural plasma-derived *** study provides an alternative strategy for large-scale production of HSA with a built-in transgene safety control mechanism.

关键词： Recombinant human serum albumin (rHSA) Selectively terminable transgenic rice Purification

Effect of the sonic hedgehog inhibitor GDC-0449 on an in vitro isogenic cellular model simulating odontogenic keratocysts

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International Journal of Oral Science 2019年第1期11卷 33-41页

作者： Jiemei Zhai Heyu zhang Jianyun zhang Ran zhang Yingying Hong Jiafei Qu Feng Chen tiejun Li Department of Oral Pathology Peking University School and Hospital of Stomatology Department of Oral Pathology School of Stomatology Kunming Medical University Central Laboratory Peking University School and Hospital of Stomatology Peking University Hospital of Stomatology First Clinical Division

Odontogenic keratocysts(OKCs) are common cystic lesions of odontogenic epithelial origin that can occur sporadically or in association with naevoid basal cell carcinoma syndrome(NBCCS). OKCs are locally aggressive, cause marked destruction of the jaw bones and have a propensity to recur. PtCH1 mutations(at ～80%) are frequently detected in the epithelia of both NBCCS-related and sporadic OKCs, suggesting that PtCH1 inactivation might constitutively activate sonic hedgehog(SHH) signalling and play a major role in disease pathogenesis. thus, small molecule inhibitors of SHH signalling might represent a new treatment strategy for OKCs. However, studies on the molecular mechanisms associated with OKCs have been hampered by limited epithelial cell yields during OKC explant culture. Here, we constructed an isogenic PtCH1^(R135 X/+) cellular model of PtCH1 inactivation by introducing a heterozygous mutation, namely, c.403 C>t(p.R135 X), which has been identified in OKC patients, into a human embryonic stem cell line using the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated 9(Cas9) system. this was followed by the induction of epithelial differentiation. Using this in vitro isogenic cellular model, we verified that the PtCH1 R135 X/+heterozygous mutation causes ligand-independent activation of SHH signalling due to PtCH1 haploinsufficiency. this activation was found to be downregulated in a dose-dependent manner by the SHH pathway inhibitor GDC-0449. In addition, through inhibition of activated SHH signalling, the enhanced proliferation observed in these induced cells was suppressed, suggesting that GDC-0449 might represent an effective inhibitor of the SHH pathway for use during OKC treatment.

关键词： NBCCS the SHH pathway inhibitor GDC-0449

Late triassic Biotite Monzogranite from the Western Litang Area,Yidun terrane,SW China: Petrogenesis and tectonic Implications

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Acta Geologica Sinica(English Edition) 2019年第2期93卷 307-321页

作者： ZHU Yu LAI Shaocong QIN Jiangfeng zhang Zezhong zhang Fangyi State Key Laboratory of Continental Dynamic Department of Geology Northwest University

the Late triassic igneous rocks in the Yidun terrane can provide vital insights into the evolution of Plaeo-tethys in western China. We present new zircon U-Pb, whole-rock geochemistry, and Sr-Nd-Pb-Hf isotopic data for the Litang biotite monzogranites, Yidun terrane. the biotite monzogranites have a zircon U-Pb age of 206.1±1.0 Ma(MSWD=1.9,n=30), which indicates Late triassic magmatism. the biotite monzogranites display I-type affinity, high Na_2O(3.38-3.60 wt%) contente,medii SiO_2(67.12-69.13 wt%), and low P_2 O_5 contents(0.10～0.12 wt%). they enriched in Rb,and Ba and depleted in Nb and ta, with negative Eu anomalies(Eu/Eu*=0.74—0.81). they have evolved Sr-Nd-Pb-Hf isotopic composition, i.e.,(^(87) Sr/^(86 )Sr)i=0.714225 to 0.714763, negative ?_(Nd(t)) values of -2.0 to-2.6 with two-stage Nd model ages ranging from 1.01 to 1.05 Ga, negative ?_(Ht)(t)) values o f-3.4 to-4.1 with two-stage Hf model ages of 1.85 to1.88 Ga, suggesting a matured crustal sources. their low Al_2O_3/tiO_2 ratios and medium Cao/Na_2O ratios, medium Mg~# and SiO_2 contents, low [molar Al_2O_3/(MgO+FeO^t)] values, and high [molar Cao/(MgO+FeO^t)] values indicate that the Litang biotite monzogranite was formed by partial melting of metabasaltic rocks. Based on the previous studies, we propose that the Litang biotite monzogranite derived from the westward subduction and closure of the Ganzi-Litang ocean during the Late triassic-the mantle wedge-derived mafic melts provided sufficient heat for partial melting of ancient metabasalt protolith within the middle-lower crust.

关键词： Late triassic biotite monzogranite zircon U-Pb dating Sr-Nd-Pb-Hf isotopes Yidun terrane

Sm-Nd dating and rare earth element geochemistry of the hydrothermal calcites from Guling carbonate-hosted talc mineralization in the central Guangxi province,South China

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Chinese Journal Of Geochemistry 2015年第2期34卷 156-166页

作者： Yi Cai Qian zhang Yongbing zhang Dapeng Wang Kaiwen Li State Key Laboratory of Ore Deposit Geochemistry Institute of GeochemistryChinese Academy of Sciences University of Chinese Academy of Sciences Henan Institute of Geological Survey

Many carbonate-hosted talc mineralization,which are widespread in South China,exclusively developed in Carboniferous dolomitic limestone with many siliceous bands and nodules,and *** of those typical deposits is the Guling talc deposit in Mashan County,central Guangxi province,with a talc reserve of1.51 million *** associations in the deposit are sample,mainly including talc and *** this paper,Sm-Nd isotopic system and rare earth elements and yttrium(REE + Y) for the hydrothermal calcite intergrown with talc are used to constrain the age and origin of the talc *** hydrothermal calcite samples from the deposit display Sm and Nd concentrations ranging from 0.18 to 0.85 and 0.85 to 4.56 ppm,respectively,and variable Sm/Nd ratios of *** calcites further yield an Sm-Nd isochron age of 232 ±19 Ma(2a)(MSWD = 0.47) with an concordant initial ^(143)Nd-^(144)Nd ratios of 0.511967 ± 0.000017,which should be interpreted as the mineralization age of the Guling talc *** addition,the calcite samples are enriched in REE with the variable SREE contents ranging from 4.82 to21.50 ppm and display relatively consistent chondritenormalized REE + Y patterns with the LREE enrichment(LREE/HREE=2.00-3.60)and the obvious negative Eu(δEu=0.52-0.68)and Ce(δCe=0.16-0.33)*** Y/Ho ratios of seven calcites varies from 43.30 to59.34,with a mean value of *** available mineral associations and REE parameters(i.e.,REE patterns and Y/Ho ratios) of those calcites indicate that the ore-forming fluids of the talc mineralization be probably derived from the meteoric waters,in particular evolved ones in the Karst areas and the ore-forming materials(e.g.,Si and Mg) are likely to be originated from the ore-bearing dolostone in the Yanguan Formation(C_1y) and underlying siliceous rocks in the Liujiang Formation(D_3l).Furthermore,the talc mineralization could take place within a hydrothermal system with relative oxidizing environment and mi

关键词：稀土元素地球化学 Sm-Nd同位素热液方解石滑石矿床中国南方广西中部碳酸盐托管

基于^(11)C-PIB PEt/MR研究APOE对阿尔茨海默病患者皮质下结构Aβ沉积和功能连接改变的影响

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中华核医学与分子影像杂志 2024年第5期44卷 285-290页

作者：常燕张熙琬吴世娜刘家金付华平张锦明刘若卓单保慈王瑞民解放军总医院第一医学中心神经内科北京100853 北京理工大学光电学院北京100081 解放军总医院第一医学中心核医学科北京100853 中国科学院高能物理研究所核技术应用研究中心北京100049

目的探讨阿尔茨海默病(AD)患者载脂蛋白E(APOE)ε4等位基因对皮质下结构的β-淀粉样蛋白(Aβ)沉积、脑区之间的功能连接(FC)的影响。方法前瞻性纳入2023年1月至2023年10月间解放军总医院第一医学中心临床诊断为很可能的轻/中度AD患者43... 详细信息

目的探讨阿尔茨海默病(AD)患者载脂蛋白E(APOE)ε4等位基因对皮质下结构的β-淀粉样蛋白(Aβ)沉积、脑区之间的功能连接(FC)的影响。方法前瞻性纳入2023年1月至2023年10月间解放军总医院第一医学中心临床诊断为很可能的轻/中度AD患者43例,包括APOEε4+23例[男12例、女11例,年龄(74.8±8.4)岁]、APOEε4-20例[男14例、女6例,年龄(77.6±8.9)岁],以及认知功能正常受试者(NC)20名[男15名、女5名,年龄(75.3±6.2)岁]。所有受试者行^(11)C-匹兹堡化合物B(PIB)PEt/MR脑显像。使用单因素方差分析、最小显著差异t检验比较3组受试者间皮质下结构(海马、杏仁核)的灰质体积(GMV)差异;采用两独立样本t检验比较APOEε4+和APOEε4-基因携带者组间Aβ沉积差异;采用Pearson相关分析ROI Aβ沉积与各脑区FC系数的相关性。结果NC组与APOEε4+基因携带者组,APOEε4+与APOEε4-基因携带者组双侧杏仁核GMV差异有统计学意义(F=6.43,P=0.002;P值:0.002、0.003);3组间双侧海马GMV差异有统计学意义(F=5.34,P=0.030)。APOEε4+和APOEε4-基因携带者组间海马和杏仁核均存在PIB异常摄取,APOEε4+组摄取更高(t值:3.14、2.19,P值:0.032、0.009)。以海马为种子点,APOEε4+、APOEε4-基因携带者及NC组的全脑连接图无明显异常。以杏仁核为种子点,APOEε4+基因携带者组全脑连接可见强度明显减弱,其在扣带回、顶叶和颞叶等脑区较NC组明显降低;而APOEε4-基因携带者组杏仁核与全脑连接强度无明显降低脑区。杏仁核Aβ沉积和杏仁核与额叶多个脑区、回直肌、右侧枕中回及左侧颞叶FC系数均呈正相关(r值:0.56~0.70,均P<0.05)。结论APOE基因型影响海马、杏仁核GMV和Aβ沉积以及杏仁核FC,并可能参与了认知功能损害的病理机制。

关键词：阿尔茨海默病淀粉样β肽类载脂蛋白E类噻唑类正电子发射断层显像术磁共振成像

基于网络药理学和分子对接探讨党参防治免疫性血小板减少症的分子机制

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护理研究 2024年第9期38卷 1505-1515页

作者：朱瑞芳陈雨露王倩张珺张淑文吕亚茹韩世范王宏伟山西医科大学第一医院山西030001 山西医科大学山西医科大学第二医院

目的:采用网络药理学、差异基因分析、分子对接方法初步阐明党参治疗免疫性血小板减少症(ItP)的分子机制。方法:在传统中药系统药理学数据库(tCMSP)、传统中药综合数据库(tCMID)和中医药百科全书数据库(EtCM)中检索党参的主要成分和相... 详细信息

目的:采用网络药理学、差异基因分析、分子对接方法初步阐明党参治疗免疫性血小板减少症(ItP)的分子机制。方法:在传统中药系统药理学数据库(tCMSP)、传统中药综合数据库(tCMID)和中医药百科全书数据库(EtCM)中检索党参的主要成分和相应的蛋白质靶标。在GeneCards、OMIM、DisGeNEt数据库收集ItP的靶点,构建韦恩图获得复合靶标和疾病的交集靶点,并将靶标导入StRING数据库,使用Cytoscape3.9.1构建PPI网络。接着对交集靶点进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析,以探索ItP与党参的相关信号通路,最后对关键靶点和活性化合物进行分子对接研究。结果:共获得党参的84种潜在活性化合物、2354个疾病靶点与257个药物靶点,并得到86个交集靶点。通过蛋白互相作用网络图(PPI)分析确定了15个关键靶点,Degree值排名前5位的靶点包括血管内皮生长因子A(VEGFA)、Src原癌基因(SRC)、白细胞介素2(IL2)、过氧化物酶体增殖激活受体γ(PPARG)、表皮生长因子受体(EGFR)。GO和KEGG分析表明,党参治疗ItP主要涉及ERK1和ERK2级联的正向调控、信号转导、蛋白质磷酸化等生物学过程,信号通路主要包括PI3K/Akt、癌症通路、t细胞信号通路。分子对接结果表明,SRC、PPARG与11-hydroxyrankinidine具有较高的结合活性。肉豆蔻酸、乙基α-d果糖呋喃糖苷、黄豆黄素是重要的活性化合物并通过分子对接模拟进行验证。结论:本研究从多个角度阐明党参可能通过调节多个靶点和多条途径对ItP产生治疗作用,可为进一步深入研究党参对ItP的作用提供科学依据。

关键词：免疫性血小板减少症党参网络药理学分子对接分子机制

Role of Extratropical Cyclones in the Recently Observed Increase in Poleward Moisture transport into the Arctic Ocean

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Advances in Atmospheric Sciences 2018年第1期35卷 85-94页

作者： Gian A.VILLAMIL-OtERO Jing zhang ,Juanxiong HE Xiangdong zhang Department of Physics North Carolina A&T State UniversityGreensboroNorth Carolina 27401USA Department of Energy and Environmental Systems North Carolina A&T State UniversityGreensboroNorth Carolina 27401USA 1nstitute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China Department of Atmospheric Science University of Alaska FairbanksFairbanksAlaska 99775USA

Poleward atmospheric moisture transport(AMt) into the Arctic Ocean can change atmospheric moisture or water vapor content and cause cloud formation and redistribution, which may change downward longwave radiation and, in turn, surface energy budgets, air temperatures, and sea-ice production and melt. In this study, we found a consistently enhanced poleward AMt across 60?N since 1959 based on the NCAR–NCEP reanalysis. Regional analysis demonstrates that the poleward AMt predominantly occurs over the North Atlantic and North Pacific regions, contributing about 57% and 32%, respectively, to the total transport. to improve our understanding of the driving force for this enhanced poleward AMt, we explored the role that extratropical cyclone activity may play. Climatologically, about 207 extratropical cyclones move across 60?N into the Arctic Ocean each year, among which about 66(32% of the total) and 47(23%) originate from the North Atlantic and North Pacific Ocean, respectively. When analyzing the linear trends of the time series constructed by using a 20-year running window, we found a positive correlation of 0.70 between poleward yearly AMt and the integrated cyclone activity index(measurement of cyclone intensity, number, and duration). this shows the consistent multidecadal changes between these two parameters and may suggest cyclone activity plays a driving role in the enhanced poleward AMt. Furthermore, a composite analysis indicates that intensification and poleward extension of the Icelandic low and accompanying strengthened cyclone activity play an important role in enhancing poleward AMt over the North Atlantic region.

关键词： atmospheric moisture transport cyclone activity atmospheric circulation Arctic climate change

Speech Intelligibility Enhancement Algorithm Based on Multi-Resolution Power-Normalized Cepstral Coefficients(MRPNCC)for Digital Hearing Aids

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Computer Modeling in Engineering & Sciences 2021年第2期126卷 693-710页

作者： Xia Wang Xing Deng Hongming Shen Guodong zhang Shibing zhang School of Information Science and technology Nantong UniversityNantong226019China School of Automation Key Laboratory of Measurement and Control for CSEMinistry of EducationSoutheast UniversityNanjing210096China School of Computer Science Jiangsu University of Science and TechnologyZhenjiang212003China

Speech intelligibility enhancement in noisy environments is still one of the major challenges for hearing impaired in everyday ***,Machine-learning based approaches to speech enhancement have shown great promise for improving speech *** key issues of these approaches are acoustic features extracted from noisy signals and classifiers used for supervised *** this paper,features are ***-resolution power-normalized cepstral coefficients(MRPNCC)are proposed as a new feature to enhance the speech intelligibility for hearing *** new feature is constructed by combining four cepstrum at different time–frequency(t–F)resolutions in order to capture both the local and contextual *** vectors and binary masking labels calculated by signals passed through gammatone filterbank are used to train support vector machine(SVM)classifier,which aim to identify the binary masking values of the t–F units in the enhancement *** enhanced speech is synthesized by using the estimated masking values and wiener filtered t–F *** experimental results demonstrate that the proposed feature is superior to other comparing features in terms of HIt-FA,StOI,HASPI and PESQ,and that the proposed algorithm not only improves speech intelligibility but also improves speech quality *** tests validate the effectiveness of the proposed algorithm for hearing impaired.

关键词： Speech intelligibility enhancement multi-resolution power-normalized cepstral coefficients binary masking value hearing impaired

H-bonding template-directed synthesis of a complete m-PDA-bridged ladder polyhydrosiloxane (OLPHS)

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Chinese Chemical Letters 2007年第7期18卷 779-782页

作者： Kui Lin Deng tao Yi zhang Xiao Jing zhang Ping Xie Rong Ben zhang State Key Laboratory on Polymer Physics ＆ Chemistry Joint Laboratory of Polymer Science ＆ MaterialsInstitute of Chemistry Chinese Academy of Sciences Beijing 100080 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China College of Chemistry ＆ Environmental Science Hebei University Baoding 071002 China

A highly ordered m-phenylenediimino-bridged ladder polyhydrosiloxane （abbr. OLPHS） with Mn = 1.24 × 10^4 was synthesized stoichiometric hydrolysis and dehydrochlorination condensation reaction between Si-Cl and Si-OH bonds. the complete ladder structure of OLPHS has been confirmed by the following three data. two characteristic Bragg＇s peaks representing the ladder width （w = 0.94 nm） and ladder thickness （t = 0.42 nm） were observed in XRD analysis, which are consistent with those calculated by molecular simulation. the very sharp absorption with a small half-peak width （w1/2 = 0.5 ppm） for [（-HN）HSiO2/2]n moiety of OLPHS in ^29Si NMR spectrum indicated presence of the complete ladder structure. As collateral evidence, a higher glass transition temperature （tg = 105 ℃） is also recorded in the DSC measurement, implying the high stiffness of ladder chain of OLPHS.

关键词： H-bonding template Ladder polysiloxane Self-assembly Stepwise coupling/polymerization