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Potential Antioxidant Activities Improve Salt Tolerance in Ten Varieties of Wheat (<i>Triticum aestivum</i>L.)

American Journal of Plant Sciences 2013年第6期4卷 69-76页

作者： Aurangzeb Rao syed Dilnawaz Ahmad syed mubashar sabir Shahid Iqbal Awan Asad Hussain Shah syed Rizwan Abbas Saima Shafique Fareed Khan Atia Chaudhary Department of Chemistry University of the Poonch Rawalakot Pakistan. Department of Plant Breeding and Molecular Genetics Faculty of Agriculture University of the Poonch Rawalakot Pakistan Department of Plant Breeding and Molecular Genetics Faculty of Agriculture University of the Poonch Rawalakot Pakistan University of Azad Jammu and Kashmir Muzaffarabad Pakistan

The activity of antioxidative enzymes system is affected by salt stress, chlorophyll content (CHL), leaf relative water content (RWC), Na+ and K+ contents, their ratio and some oxidative stress indices were studied in leaves of ten bread wheat cultivarsSehar-06, Lu-26, NARC-09, BARC-09 and Pirsbak-09’(salt-tolerant) and Kaghan-94, Rohtas-90, Soughat-90, Shaheen-94 and Zardana-89’(salt-sensitive), grown under salinity treatments carried out in five levels (1 dS·m-1 as control, 2, 4, 8, 16 dS·m-1) via sodium chloride. Under high salt potency significant increase for activities of antioxidant enzymes such as ascorbate peroxidase (APX) and guiacol peroxidase (GPX), occured in salt tolerant varieties. Meanwhile, under salinity condition the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and (GPX) in sensitive cultivar were lower than control. Regarding (APX) activity there was no significant difference between salinity and control situation. Under salt stress membrane stability index (MSI) of both cultivars were negatively influenced. Hydrogen peroxide (H2O2) content of salinity sensitive cultivars was higher than control. Salt tolerant varieties had more amounts of K+ content, K+ and Na+ ratio, relative water content, yield and chlorophyll under salt conditions, and sensitive ones recorded higher Na+ content at tillering stage. The mechanism of salt stress might be achieved due to low lipid peroxidation, assumingly lower changes in membrane stability index and evasion of Na+ combination and amplified activity of antioxidant enzymes.

关键词： Antioxidant Enzymes Chlorophyll Content+/Na+ Ratio Relative Water Content Salinity Wheat

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2D Carbon Fiber Reinforced High Density Polyethylene Multi-Layered Laminated Composite Panels:Structural,Mechanical,Thermal,and Morphological Profile

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Journal of Materials Science & Technology 2016年第10期32卷 1077-1082页

作者： Shahzad Maqsood Khan Nafisa Gull Muhammad Azeem Munawar Atif Islam Saba Zia Muhammad Shafiq Aneela sabir syed Muhammad Awais Muhammad Arif Butt Muhammad Taqi Zahid Butt Tahir Jamil Department of Polymer Engineering and Technology University of the PunjabLahore 54590Pakistan King Edward Medical University Lahore 54590Pakistan College of Engineering and Emerging Technologies University of the PunjabLahore 54590Pakistan

Carbon fiber reinforced high density polyethylene multi-layered laminated composite panels（HDPE/CF MLCP） with excellent in-plane properties along transverse direction have been formulated. Composite architectures with carbon fiber（CF） designed in 2D layout in conventional composites can alleviate their properties in thickness direction, but all attempts so far developed have achieved restrained success. Here,we have exposed an approach to the high strength composite challenge, without altering the 2D stack design on the basis of concept of fiber reinforced laminated composites that would provide enhanced mechanical and thermal properties along transverse direction. CF sheets allowed the buckling of adjoining plies in 2D MLCP. We fabricated 2D MLCP by stacking the alternative CF and HDPE layers under different loading conditions, which resulted in high strength composites. These plies of CF and HDPE served as unit cells for MLCP, with CF offering much-needed fracture toughness and hardness to these *** 2D HDPE/CF MLCP, we demonstrated noteworthy improvement in physical and chemical interaction between CF and HDPE, in-plane fracture strain, flexural strength（30.684 MPa）, bending modulus（7436.254 MPa）, thermal stability（40.94%）, and surface morphology, upon increasing the CF layers up to twenty, enabling these composites truly for high temperature and high strength applications.

关键词： Multi-layered composite panels Mechanical properties Thermal stability

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Microrna 27a-3p regulates antimicrobial responses of Murine Macrophages infected by Mycobacterium avium subspecies paratuberculosis by Targeting interleukin-10 and TgF-β-activated Protein Kinase 1 Binding Protein 2

Microrna 27a-3p regulates antimicrobial responses of Murine ...

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中国畜牧兽医学会兽医病理学分会第二十四次学术研讨会、中国病理生理学会动物病理生理学专业委员会第二十三次学术研讨会、中国实验动物学会实验病理学专业委员会第三次学术研讨会、中国兽医病理学家第三次学术研讨会

作者： Tariq Hussain Deming Zhao syed Zahid Ali Shah Jie Wang Ruichao Yue Yi Liao Naveed sabir Lifeng Yang Xiangmei Zhou National Animal Transmissible Spongiform Encephalopathy Laboratory College of Veterinary Medicine China Agricultural University

Mycobacterium avium subspecies paratuberculosis(MAP) persistently survive and replicate in macrophages by adopting various strategies to subvert host immune response. Interleukin-10(IL-10) upregulated via MAPK-p38 signaling cascade is a critical step for MAP survival and pathogenesis within the host cell. In the current study, we found that miR-27 a expression is downregulated during MAP infection both in vivo and in vitro. We observed that overexpression of miR-27 a inhibited MAP induced IL-10 production in macrophages and upregulated proinflammatory cytokines, while miR-27 a inhibitor counteracted these effects. Luciferase reporter assay results revealed that IL-10 and TAB2 are potential targets of miR-27 a. Furthermore, overexpression of miR-27 a significantly inhibited the intracellular survival of MAP in infected macrophages. Our data shows that miR-27 a augments antimicrobial activities of macrophages and inhibits the expression of IL-10, demonstrating that miR-27 a regulates protective innate immune responses during MAP infection and can be exploited as a novel therapeutic target in the control of intracellular pathogens, including MAP.

关键词： Mycobacterium avium subspecies paratuberculosis (MAP) MicroRNAs (miRNAs) miR-27a Mitogen activated protein kinase p38 (MAPK-p38) TAK1 binding protein 2 (TAB2) interleukin-10 (IL-10)

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