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Decoding bovine coronavirus immune targets:an epitope informatics approach

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Animal Diseases 2024年第2期4卷 138-153页

作者： swati Rani Mehnaj Khatoon Jagadish Hiremath Kuralayanapalya Puttahonnappa suresh Jayashree Anandakumar Nagendra Nath Barman sheethal Manjunath Yamini sri s sharanagouda s.Patil spatial Epidemiology Lab ICAR-National Institute of Veterinary Epidemiology and Disease InformaticsYelahankaBengaluruKarnataka 560064India College of Veterinary science Assam Agricultural UniversityGuwahatiAssam 785013India Yuvaraja’s College Mysuru University of MysuruMysuruKarnataka 570005India

Bovine coronavirus(BCoV)poses a significant threat to the global cattle industry,causing both respiratory and gastrointestinal infections in cattle *** necessitates the development of efficacious *** several inactivated and live BCoV vaccines exist,they are predominantly limited to *** immunization of adult cattle is imperative for BCoV infection control,as it curtails viral transmission to calves and ameliorates the impact of enteric and respiratory ailments across all age groups within the *** study presents an in silico methodology for devising a multiepitope vaccine targeting *** spike glycoprotein(s)and nucleocapsid(N)proteins,which are integral elements of the BCoV structure,play pivotal roles in the viral infection cycle and immune *** constructed a remarkably effective multiepitope vaccine candidate specifically designed to combat the BCoV *** immunoinformatics technology,B-cell and T-cell epitopes were predicted and linked together using linkers and adjuvants to efficiently trigger both cellular and humoral immune responses in *** in silico construct was characterized,and assessment of its physicochemical properties revealed the formation of a stable vaccine *** 3D modeling of the vaccine construct,molecular docking revealed a stable interaction with the bovine receptor ***,the viability of the vaccine’s high expression and simple purification was demonstrated by codon optimization and in silico cloning expression into the pET28a(+)*** applying immunoinformatics approaches,researchers aim to better understand the immune response to bovine coronavirus,discover potential targets for intervention,and facilitate the development of diagnostic tools and vaccines to mitigate the impact of this virus on cattle health and the livestock *** anticipate that the design will be useful as a preventive treatment for BCoV sickness in cattle,opening the door for furthe

关键词： Immunoinformatics Bovine coronavirus Multiepitope vaccine Molecular docking In silico cloning

Rice-wheat comparative genomics:Gains and gaps

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The Crop Journal 2024年第3期12卷 656-669页

作者： Akila Wijerathna-Yapa Ruchi Bishnoi Buddhini Ranawaka Manu Maya Magar Hafeez Ur Rehman swati G.Bharad Michal T.Lorenc Vinita Ramtekey sasha Gohar Charu Lata Md.Harun-Or-Rashid Maryam Razzaq Muhammad sajjad Bhoja *** school of Agriculture and Food sustainability The University of QueenslandSt LuciaQLD 4072Australia ARC Centre of Excellence for Plant success in Nature and Agriculture The University of QueenslandSt LuciaQLD 4072Australia Department of Genetics and Plant Breeding College of AgricultureUmmedganj-KotaAgriculture UniversityKotaRajasthan 324001India Centre for Agriculture and the Bioeconomy Queensland University of Technology(QUT)2 George StreetBrisbaneQLD 4000Australia UWA school of Agriculture and Environment The University of Western Australia35 Stirling HighwayCrawleyWA 6009Australia Nepal Agriculture Research Council KhumaltarLalitpurNepal Department of Agronomy University of AgricultureFaisalabad 38040Pakistan Department of Agricultural Botany Dr Panjabrao Deshmukh Krishi VidyapeethAkola 444104MaharashtraIndia ICAR-Indian Institute of seed science MauUttar Pradesh 275103India school of Natural sciences Macquarie UniversityMacquarie ParkNSW 2109Australia ICAR-Indian Institute of Wheat and Barley Research Flowrdale 54642ShimlaIndia International Maize and Wheat Improvement Center(CIMMYT)Bangladesh Office Gulshan-2Dhaka 1212Bangladesh Center for seed science University of AgricultureFaisalabad 38000Pakistan Department of Biosciences COMSATS University IslamabadPark RoadTarlai KalanIslamabad 45550Pakistan CGIAR’s Excellence in Breeding(EiB)Platform/Accelerated Breeding Initiative International Maize and Wheat Improvement Center(CIMMYT)El Batán 56237Mexico

Rice and wheat provide nearly 40%of human calorie and protein *** share a common ancestor and belong to the Poaceae(grass)*** their genetic homology is crucial for developing new cultivars with enhanced *** wheat genes and gene families have been characterized based on their rice ***–wheat orthology can identify genetic regions that regulate similar traits in both ***–wheat comparative genomics can identify candidate wheat genes in a genomic region identified by association or QTL mapping,deduce their putative functions and biochemical pathways,and develop molecular markers for marker-assisted breeding.A knowledge of gene homology facilitates the transfer between crops of genes or genomic regions associated with desirable traits by genetic engineering,gene editing,or wide crossing.

关键词： Comparative genomics Orthologs Genes synteny Genetic engineering Molecular breeding

The potential role of essential oils in boosting immunity and easing COVID-19 symptoms

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Clinical Traditional Medicine and Pharmacology 2024年第2期5卷 54-62页

作者： Vivek P Chavda Pankti C.Balar Gargi Jogi swati Marwadi Aayushi Patel Avani Doshi Jinal Ajabiya Lalitkumar Vora Department of Pharmaceutics and Pharmaceutical Technology L.M.College of PharmacyAhmedabad 380009GujaratIndia Department of Biotechnology Savitribai Phule University of Pune411041MaharashtraIndia Pharmacy section Long Island UniversityUniversity PlazaBrooklynNY 11201United States Department of Pharmaceutical Chemistry K B Raval College of PharmacyGandhinagar 382423India Department of Pharmaceutics Analysis National Institute of Pharmaceutical Education and Research(NIPER)Ahmedabad 380009GujaratIndia school of Pharmacy Queen’s University Belfast97 Lisburn RoadBT97BLUK

Background:several trials have been conducted since the beginning of the COVID-19 pandemic to establish possible clinical treatments for the infected ***,many these approaches have failed to demonstrate positive results in human clinical *** the middle of this investigation,essential oils attracted interest due to their numerous benefits and possible role in management of *** oils have virucidal effects at nontoxic quantities due to their tiny molecular size,high lipophilicity,and quick commencement of ***,the pulmonary exhalation,lower respiratory stimulus,and deep lung transport properties of essential oils increase their potential medicinal ***:The present review aimd to evaluate the potential efficacy of essential oil in management of ***:Following the Preferred Reporting Items for systematic Review and Meta-Analysis(PRIsMA)guidelines,studies published in English up to January 2024 were searched in the PubMed,scifinder,and scopus database,using relevant keywords,such as“Essential oil and COVID-19”,“Essential Oil and Viral Infection”,*** non-English articles,abstracts,conference papers,book chapters,and reviews were ***:A total of 108 potentially relevant articles were included for full-text review,with the emphasis is on scrutinizing the probable antiviral action of essential oils to inhibit the replication of *** tried to provide an opinion based on different studies performed using the same concept in different corners of the ***:Various essential oils hold a great potential to work as an adjuvant therapy in management and prevention of COVID-19.

关键词： COVID-19 sARs-CoV-2 sunlight UV radiation Essential oil

LRs Bianchi Type-II Inflationary Universe with Massless scalar Field and Time Varying Λ

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Chinese Physics Letters 2012年第8期29卷 42-44页

作者： Raj Bali swati Department of Mathematics University of RajasthanJaipur 302004India

The locally rotationally symmetric(LRs)Bianchi type-II inflationary cosmological model is investigated for massless scalar field with flat potential and time varyingΛ.To obtain the deterministic solution,it is assumed that scale factor is a(t)~e^(Ht) as we considered previously for Bianchi type-I spacetime andΛ~a^(-2) as considered by Chen and Wu,where H is the Hubble constant and effective potential V(φ)=const;φHigg's *** is shown that such a time varyingΛleads to no conflict with existing ***,it does change the predictions of standard cosmology in the matter-dominated phase and alleviates some problems in reconciling observations with the inflationary *** model represents anisotropic spacetime in ***,the model isotropizes for large values of t andβ=3H^(2),whereβis *** physical and geometrical aspects of the model in the context of an inflationary scenario is also discussed.

关键词： scenario field Bianchi

Impact of Cumulative Exposure to Circulating Ovarian sex Hormones on Increasing the Risk of Hormone Receptor-Positive Breast Cancer

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Journal of Bio-X Research 2024年第1期7卷 36-43页

作者： swati sucharita Mohanty sumit subhadarshi Mohanty soumya surath Panda Chita Ranjan sahoo Prafulla Kumar Mohanty Rabindra Nath Padhy siksha O Anusandhan University Institute of Medical sciences and sUM Hospital BhubaneswarOdishaIndia Regional Medical Research Centre Bhubaneswar BhubaneswarOdishaIndia Khallikote Unitary University BrahmapurOdishaIndia

There were 2.3 million new cases of breast cancer registered in 2020,establishing it as the world’s leading *** regular exposure of breast cells to circulating ovarian sex hormones may contribute to cancer growth at all stages.A literature review was performed by downloading research articles from PubMed Central,with keywords such as breast cancer,hormone exposure in breast cancer,estrogen/progesterone exposure in breast cancer,and breast cancer risk *** breast cancer is a hormone-related disease,several factors that influence hormonal conditions or are indicators of alterations in hormonal conditions have been linked with the risk for breast ***,an elevated risk for breast cancer has been related to reproductive history and exogenous estrogen exposure because they may amplify lifetime estrogen *** these factors address the total cumulative exposure to circulating ovarian sex hormones that a female is vulnerable to in her lifetime.A healthy lifestyle,a more extended breastfeeding period,and regular physical activity may help lower one’s *** review summarizes the factors that increase the risk of estrogen/progesterone(hormone receptor)receptor-positive breast cancer due to overall cumulative estrogen exposure,with an understanding of estrogen biosynthesis mechanisms and estrogen receptor signaling.

关键词： alterations elevated exposure

Laboratory investigation of compaction characteristics of fresh and degraded municipal solid waste

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Waste Disposal and sustainable Energy 2020年第4期2卷 305-312页

作者： Mahesh Endait swati Patil Department of Civil Engineering School of Engineering and TechnologySandip UniversityNashikIndia

com-pacted MsW increases the placement efficiency and thus reduces the space requirement for *** composition of MsW,degradation,and compactive efforts are the key factors that control the compaction characteristics of *** paper presents the laboratory investigation of compaction characteristics of fresh and aged MsW at a wide range of compactive *** MsW(s_(1)),Windrows sample(s_(2))of an age of 2 months,5-year-old MsW(s_(3)),10-year-old MsW(s_(4)),and 15-year-old MsW(s_(5))were collected from the working phase of the solid waste management site,Vilholi Nasik,(MH)*** was carried in 1000 mL capacity mold under four different energy levels,i.e.,552(E_(1)),1125(E_(2)),2682(E_(3)),and 5364(E4)kJ/m^(3).The results showed that the maximum dry density(γ_(dmax))was observed less in the s_(1)sample at the standard compactive *** the age of the sample increase from fresh to 5 years,γ_(dmax)was.more with lesser optimum moisture content(w_(opt))because of the completion of the biodegradation *** further change in compaction characteristics was observed once the biodegradation process was *** study shows that the particle size of MsW gets reduced as the age of the sample *** reduces the particle size of MsW is one of the contributing factors to increasingγ_(dmax).When compactive energy is changed from E_(1)to E4 theγ_(dmax)increased and w_(opt)decreased for all the samples.A normalized w_(opt)and compaction energy correlation is proposed in this *** correlation can get compaction characteristics of fresh and aged MsW at any compactive effort.

关键词： Compaction Municipal solid waste Degradation Compaction efforts

Upconversion Nanoparticles-Encoded Hydrogel Microbeads-Based Multiplexed Protein Detection

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Nano-Micro Letters 2018年第2期10卷 135-150页

作者： swati shikha Xiang Zheng Yong Zhang Department of Biomedical Engineering Faculty of Engineering National University of Singapore (NUS) NUs Graduate school for Integrative sciences and Engineering Centre for Life Sciences (CeLS)

Fluorescently encoded microbeads are in demand for multiplexed applications in different *** to organic dye-based commercially available Luminex's x MAP technology, upconversion nanoparticles(UCNPs) are better alternatives due to their large antistokes shift, photostability, nil background, and single wavelength excitation. Here, we developed a new multiplexed detection system using UCNPs for encoding poly(ethylene glycol) diacrylate(PEGDA) microbeads as well as for labeling reporter antibody. However, to prepare UCNPs-encoded microbeads, currently used swellingbased encapsulation leads to non-uniformity, which is undesirable for fluorescence-based multiplexing. Hence,we utilized droplet microfluidics to obtain encoded microbeads of uniform size, shape, and UCNPs distribution inside. Additionally, PEGDA microbeads lack functionality for probe antibodies conjugation on their *** to functionalize the surface of PEGDA microbeads(acrylic acid incorporation, polydopamine coating)reported thus far quench the fluorescence of UCNPs. Here,PEGDA microbeads surface was coated with silica followed by carboxyl modification without compromising the fluorescence intensity of UCNPs. In this study, droplet microfluidics-assisted UCNPs-encoded microbeads of uniform shape, size, and fluorescence were *** color codes were generated by mixing UCNPs emitting red and green colors at different ratios prior to encapsulation. UCNPs emitting blue color were used to label the reporter antibody. Probe antibodies were covalently immobilized on red UCNPs-encoded microbeads for specific capture of human serum albumin(HsA) as a model protein. The system was also demonstrated for multiplexed detection of both human C-reactive protein(hCRP) and HsA protein by immobilizing anti-h CRP antibodies on green UCNPs.

关键词： Upconversion nanoparticles PEGDA microbeads Encoding Multiplexed bio-detection single wavelength excitation

simultaneous quantification of prodrug oseltamivir and its metabolite oseltamivir carboxylate in human plasma by LC-Ms/Ms to support a bioequivalence study

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Journal of Pharmaceutical Analysis 2013年第3期3卷 149-160页

作者： Ajay Gupta swati Guttikar Pranav s. shrivastav Mallika sanyal Chemistry Department Kadi Sarva Vishwavidyalaya Sarva Vidyalaya Campus Sector 15/23Gandhinagar 382015 India Bioanalytical Research Department Veeda Clinical ResearchAmbawadiAhmedabad 380015 India Department of Chemistry School of Sciences Gujarat UniversityAhmedabad 380009 India Chemistry Department St. Xavier's CollegeNavrangpuraAhmedabad 380009 India

A simple, precise and rapid liquid chromatography-tandem mass spectrometry （LC-Ms/Ms） method has been developed and validated for the simultaneous determination of oseltamivir and oseltamivir carboxylate, a neuraminidase inhibitor, using their deuterated analogs as internal standards （Iss）. The method involved solid phase extraction of the analytes and Iss from 200 μL human plasma with no reconstitution and drying steps. The chromatographic separation was achieved on a symmetry C18 （100 mm × 4.6 mm, 5 μm） column using 10 mM ammonium formate and acetonitrile （30：70, v/v） as the mobile phase in a run time of 2.0 min. Quantitation of analytes and Iss were done by multiple reaction monitoring on a triple quadrupole mass spectrometer in the positive ionization mode. The linearity of the method was established in the concentration range of 0.5-200 ng/mL and 2.0-800 ng/mL for oseltamivir and oseltamivir carboxylate respectively. The mean extraction recovery for oseltamivir （94.4%） and oseltamivir carboxylate （92.7%） from spiked plasma samples was consistent and reproducible. The application of this method was demonstratedby a bioequivalence study in 42 healthy Indian subjects with 75 mg oseltamivir phosphate capsules. The assay reproducibility was established by reanalysis of 151 incurred subject samples.

关键词： Oseltamivir Oseltamivir carboxylate LC-Ms/Ms Human plasma Bioequivalence study

Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants

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Journal of Animal science and Biotechnology 2014年第2期5卷 222-229页

作者： shailendra Raghuwanshi swati Misra Rajendra Kumar saxena Department of Microbiology University of Delhi South CampusBenito Juarez Road New Delhi 110 021India

Background： Current research to enrich cattle feed has primarily focused on treatment using white rot fungi, while there are scarce reports using the enzyme tannase, which is discussed only in reviews or in the form of a hypothesis. In this context, the aim of the present study was to evaluate the effect of tannase on wheat straw （Ws） and also the effect of lyophilized tannase at concentrations of 0.1%, 0.2%, and 0.3% （w/w） on Ws followed by fermentation with Ganodermo sp. for 10 d and compared in relation to biochemical parameters, crude protein （CP） content, and nutritional value by calculating the C/N ratio in order to improve the nutritional value of cattle feed. Results： Penicillium charlesii, a tannase-producing microorganism, produced 61.4 IU/mL of tannase in 54 h when 2% （w/v） tannic acid （TA） was initially used as a substrate in medium containing （% w/v） sucrose （1.0）, NaNO3 （1.0）, and MgsO4 （0.08 pH, 5.0） in a 300-L fermentor （working volume 220 L）, and concomitantly fed with 1.0% （w/v） TA after 24 h. The yield of partially purified and lyophilized tannase was 5.8 IU/mg. The tannin-free myco-straw at 0.1% （w/w） tannase showed 37.8% （w/w） lignin degradation with only a 20.4% （w/w） decrease in cellulose content and the in vitro feed digestibility was 32.2%. An increase in CP content （up to 1.28-fold） along with a lower C/N ratio of 25.0%, as compared to myco-straw, was obtained. Conclusions： The use of tannin-free myco-straw has potential to improve the nutritional content of cattle feed. This biological treatment process was safe, eco-friendly, easy to perform, and was less expensive as compared to other treatment methods.

关键词： Ganoderma sp High energy cattle feed Tannase Tannin free myco-straw