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Prion Protein Binds to Aldolase A Produced by Bovine Intestinal M Cells

Open Journal of Veterinary Medicine 2015年第3期5卷 43-60页

作者： Yuya Nagasawa Yu Takahashi Wataru Itani Hitoshi Watanabe Yusuke Hidaka Shotaro Morita Kei suzuki Kouichi Watanabe Shyuichi Ohwada Haruki Kitazawa Morikazu Imamura takashi Yokoyama Motohiro Horiuchi Suehiro Sakaguchi Shirou Mohri Michael T. Rose Tomonori Nochi Hisashi Aso Cellular Biology Laboratory Tohoku University Sendai Japan Food Immunology Group Tohoku University Sendai Japan Prion Disease Research Center National Institute of Animal Health Tsukuba Japan Laboratory of Veterinary Hygiene Hokkaido University Sapporo Japan Institute for Enzyme Research The University of Tokushima Tokushima Japan Institute of Biological Environmental and Rural Sciences Aberystwyth University Aberystwyth UK

Microfold (M) cells are a kind of intestinal epithelial cell in the follicle-associated epithelium (FAE) of Peyer’s patches. They can transport antigens and microorganisms to lymphoid tissues. Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder in cattle. It is linked to variant Creutzfeldt-Jakob disease in humans. Although it is thought that M cells transport the BSE agent, the exact mechanism by which it crosses the intestinal barrier is not clear. We have bovine intestinal epithelial cell line (BIE cells), which can differentiate into the M cell type in vitro after stimulation, and which is able to transport the BSE agent. We show here that M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles, compared with non-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Aldolase A was co-stained with incorporated PrPSc in M-BIE cells. These results suggest that bovine M cells and goblet cells synthesize aldolase A, and that aldolase A may have the ability to bind PrP and associate with PrP in cellular vesicles. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.

关键词： Peyer’s Patch M Cell BIE Cells Aldolase A PrP Binding Protein

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A rare case of hyaline-type Castleman disease in the liver

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World Journal of Hepatology 2013年第7期5卷 404-408页

作者： Hisaaki Miyoshi Shima Mimura Takako Nomura Joji Tani Asahiro Morishita Hideki Kobara Hirohito Mori Hirohito Yoneyama Akihiro Deguchi takashi Himoto Naoki Yamamoto Keiichi Okano Yasuyuki suzuki Tsutomu Masaki Department of Gastroenterology and Neurology Faculty of Medicine Kagawa University Department of Gastroenterological Surgery Faculty of Medicine Kagawa University

Castleman disease often develops in the neck, mediastinum and pulmonary hilum. Its onset in the peritoneal cavity is very rare. The patient, a woman in her 70s, was referred to our department for a detailed examination of an abdominal mass. On abdominal ultrasonography, computed tomography scan, magnetic resonance imaging and positron emission tomography, a mass approximately 15 mm in diameter was noted in the hepatic S6. We attempted radical treatment and conducted a laparoscope-assisted right lobectomy. On the basis of histopathological findings, the patient was diagnosed as having hyaline type Castleman disease in the liver, a very rare condition.

关键词： Castleman disease Hyaline type Liver tumor Hepatectomy Positron emission tomography

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转染及培养条件对外源性钙离子ATP酶SERCA1a表达的影响

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中国组织化学与细胞化学杂志 2014年第3期23卷 223-226页

作者：王国丽大宝贵嗣山崎和生 Stefania Danko 铃木裕中国医科大学生物化学与分子生物学教研室沈阳110001 旭川医科大学

目的评估不同转染及培养条件下COS1细胞过表达钙离子ATP酶SERCA1a的量及活性,探讨影响真核细胞目标蛋白质表达量及活性的因素。方法应用常规的全量或者半量DNA、lipofactamin和plus reagent转染COS1细胞,37℃、34℃或31℃培养2至5d。提... 详细信息

目的评估不同转染及培养条件下COS1细胞过表达钙离子ATP酶SERCA1a的量及活性,探讨影响真核细胞目标蛋白质表达量及活性的因素。方法应用常规的全量或者半量DNA、lipofactamin和plus reagent转染COS1细胞,37℃、34℃或31℃培养2至5d。提取微粒体蛋白后定量并测定SERCA1a的ATP酶活性。结果微粒体蛋白量在细胞培养3至4d达到高峰,改变转染及细胞培养条件,未见明显变化;降低细胞培养温度及提高DNA转染用量,可增加SERCA1a表达量;SERCA1a表达量在8μg DNA转染细胞31℃培养3d达到最高,然而降低细胞培养温度后,SERCA1a的ATP酶活性及EP生成量也随之下降;31℃表达的SERCA1a,其ATP酶活性降低比EP生成量减少的幅度更大,并且ATP酶活性及EP生成量随着细胞培养时间延长而增加。结论应用常规的半量转染试剂、全量DNA瞬时转染COS1细胞,37℃培养3-4d可获得最大量具有正常酶活性的SERCA1a蛋白。降低培养温度虽然可以提高外源性SERCA1a的表达,但却可能影响细胞内蛋白质的准确折叠及正常降解。

关键词：钙离子ATP酶 SERCA1a 转染细胞培养

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Application of appropriate use criteria for percutaneous coronary intervention in Japan

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World Journal of Cardiology 2016年第8期8卷 456-463页

作者： Taku Inohara Shun Kohsaka Ikuko Ueda takashi Yagi Yohei Numasawa Masahiro suzuki Yuichiro Maekawa Keiichi Fukuda Department of Cardiology Hiratsuka City Hos-pital Department of Cardiology Keio University School of Medicine Keio inter--hospital Cardiovascular Studies

The aim of this review was to summarize the concept of appropriate use criteria(AUC) regarding percutaneous coronary intervention(PCI) and document AUC use and impact on clinical practice in Japan, in comparison with its application in the United States. AUC were originally developed to subjectively evaluate the indications and performance of various diagnostic and therapeutic modalities, including revascularization techniques. Over the years, application of AUC has significantly impacted patient selection for PCI in the United States, particularly in non-acute settings. After the broad implementation of AUC in 2009, the rate of inappropriate PCI decreased by half by 2014. The effect was further accentuated by incorporation of financial incentives(e.g., restriction of reimbursement for inappropriate procedures). On the other hand, when the United States-derived AUC were applied to Japanese patients undergoing elective PCI from 2008 to 2013, about one-third were classified as inappropriate, largely due to the perception gap between American and Japanese experts. For example, PCI for low-risk non-left atrial ascending artery lesion was more likely to be classified as appropriate by Japanese standards, and anatomical imaging with coronary computed tomography angiography was used relatively frequently in Japan, but no scenario within the current AUC includes this modality. To extrapolate the current AUC to Japan or any other region outside of the United States, these local discrepancies must be taken into consideration, and scenarios should be revised to reflect contemporary practice. Understanding the concept of AUC as well as its perception gap between different counties will result in the broader implementation of AUC, and lead to the quality improvement of patients' care in the field of coronary intervention.

关键词： Appropriate use criteria Acute coronary syndrome Percutaneous coronary intervention Japan Stable ischemic heart disease

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胆道肿瘤临床实践指南(英文第三版)

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中华消化外科杂志 2021年第4期20卷 359-375页

作者： Masato Nagino Satoshi Hirano Hideyuki Yoshitomi Taku Aoki Katsuhiko Uesaka Michiaki Unno Tomoki Ebata Masaru Konishi Keiji Sano Kazuaki Shimada Hiroaki Shimizu Ryota Higuchi Toshifumi Wakai Hiroyuki Isayama Takuji Okusaka Toshio Tsuyuguchi Yoshiki Hirooka Junji Furuse Hiroyuki Maguchi Kojiro suzuki Hideya Yamazaki Hiroshi Kijima Akio Yanagisawa Masahiro Yoshida Yukihiro Yokoyama takashi Mizuno Itaru Endo 杨翼飞(译) 仇毓东(译) 赵梦珂(译) 伏旭(译) 蔡正华(译) 毛凉(审校) 爱知癌症中心日本名古屋北海道大学医学院胃肠外二科日本札幌独协医科大学琦玉医学中心普通外科日本越谷独协医科大学普通外二科日本米布静冈癌症中心肝胆胰外科日本长崎东北大学医学院外科系日本仙台名古屋大学医学院外科系日本名古屋国立癌症中心医院东院区肝胆胰外科日本柏市京都大学医学院外科系日本东京国立癌症中心医院日本东京帝京大学千叶医学中心外科系日本市原东京女子医科大学胃肠病研究所普通外科日本东京新泻大学医学与牙科研究生院消化与普通外科日本新泻均天道大学医学研究生院消化内科日本东京国立癌症中心医院肝胆胰肿瘤科日本东京千叶县锯原医院消化内科日本锯原藤田医科大学消化内科及消化肿瘤科日本丰明杏林大学医学院肿瘤内科日本三鹰泰恩金凯医院教育研究中心日本札幌爱知医科大学放射科日本长谷京都府立医科大学医学研究生院放射科日本京都广崎大学医学研究部病理学与生物科学系日本广崎日本红十字会京都第二医院病理科日本京都国际医疗福利大学肝胆胰胃肠外科日本一川横滨市立大学医学研究生院消化外科日本横滨南京大学医学院附属鼓楼医院肝胆胰中心

日本肝胆胰外科学会(JSHBPS)于2007年出版第一版胆道肿瘤(胆管癌、胆囊癌及壶腹癌)临床实践指南,于2014年更新第二版,2021年英文第三版胆道肿瘤临床实践指南围绕6个主题提出若干临床问题,基于相关循证医学证据并组织专家讨论后,最终确... 详细信息

日本肝胆胰外科学会(JSHBPS)于2007年出版第一版胆道肿瘤(胆管癌、胆囊癌及壶腹癌)临床实践指南,于2014年更新第二版,2021年英文第三版胆道肿瘤临床实践指南围绕6个主题提出若干临床问题,基于相关循证医学证据并组织专家讨论后,最终确定推荐意见、推荐强度以及推荐说明。根据证据推荐分级的评估、制订与评价(GRADE)系统,推荐强度被分为1级(强)或者2级(弱)。英文第三版胆道肿瘤临床实践指南中提出的31个临床问题涵盖:(1)预防性治疗;(2)诊断;(3)胆道引流;(4)外科治疗;(5)化疗;(6)放疗。31个临床问题中,14个问题给予推荐强度为强的推荐意见,14个问题给予推荐强度为弱的推荐意见,剩余的3个问题未给予推荐意见。每条推荐意见都进行推荐强度说明。最新版指南基于循证医学证据,为临床提供了重要建议。未来与癌症登记数据库协作将是评估指南和建立新证据的关键。

关键词：胆道肿瘤临床实践指南预防性治疗循证医学证据肝胆胰外科胆道引流推荐意见壶腹癌

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在膳食诱导的肥胖小鼠中,硝苯地平改善缺血引起的血管重建过程

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中华高血压杂志 2012年第6期20卷 598-598页

作者： Tetsutaro Kito Rei Shibata Megumi Kondo takashi Yamamoto Hirohiko suzuki Masakazu Ishii Toyoaki Murohara 罗冬梅叶鹏

肥胖是心血管疾病的危险因素之一，与血管功能受损相关。硝苯地平作为钙拮抗剂的一种，有许多除了降低血压以外的功能，如改善内皮细胞功能，降低氧化应激。

关键词：肥胖小鼠血管重建硝苯地平膳食诱导缺血内皮细胞功能心血管疾病危险因素

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Repeat endoscopic submucosal dissection for recurrent gastric cancers after endoscopic submucosal dissection

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World Journal of Gastrointestinal Endoscopy 2013年第12期5卷 600-604页

作者： Yuto Shimamura Naoki Ishii Kaoru Nakano takashi Ikeya Kenji Nakamura Koichi Takagi Katsuyuki Fukuda Koyu suzuki Yoshiyuki Fujita Department of Gastroenterology St. Luke’s International Hospital Department of Pathology St. Luke’s International Hospital

AIM:To clarify the safety and efficacy of repeat endoscopic submucosal dissection(re-ESD)for locally recurrent gastric cancers after ***:A retrospective evaluation was performed of the therapeutic efficacy,complications and followup results from ESD treatment for early gastric cancers in 521 consecutive patients with 616 lesions at ***`s International Hospital between April 2004 and November *** addition,tumor size,the size of resected specimens and the operation time were compared between re-ESD and initial ESD procedures.A flex knife was used as the primary surgical device and a hook knife was used in cases with severe fibrosis in the submucosal *** variables were analyzed using the non-parametric Mann-Whitney U test and are expressed as medians(range).Categorical variables were analyzed using a Fisher’s exact test and are reported as *** significance was defined as a P-value less than ***:The number of cases in the re-ESD group and the initial ESD group were 5 and 611,*** median time interval from the initial ESD to re-ESD was 14(range,4-44 mo).En bloc resection with free lateral and vertical margins was successfully performed in all re-ESD cases without any *** local or distant recurrence was observed during the median follow-up period of 48(range,11-56 mo).Tumor size was not significantly different between the re-ESD group and the initial ESD group(median 22 mm vs 11mm,P=0.09),although the size of resected specimens was significantly larger in the re-ESD group(median 47 mm vs 34 mm,P<0.05).There was a nonsignificant increase observed in re-ESD operation time compared to initial ESD(median 202 min vs 67 min,respectively,P=0.06).CONCLUSION:Despite the low patient number and short follow-up,the results suggest that re-ESD is a safe and effective endoscopic treatment for recurrent gastric cancer after ESD.

关键词： Endoscopic submucosal dissection Recurrent gastric cancer Gastric cancer Endoscopic mucosal resection Therapeutic endoscopy

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Systemic hemodynamics in advanced cirrhosis: Concerns during perioperative period of liver transplantation

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World Journal of Hepatology 2016年第25期8卷 1047-1060页

作者： Tomohide Hori Yasuhiro Ogura Yasuharu Onishi Hideya Kamei Nobuhiko Kurata Motoshi Kainuma Hideo Takahashi Shogo suzuki takashi Ichikawa Shoko Mizuno Tadashi Aoyama Yuki Ishida Takahiro Hirai Tomoko Hayashi Kazuko Hasegawa Hiromu Takeichi Atsunobu Ota Yasuhiro Kodera Hiroyuki Sugimoto Taku Iida Shintaro Yagi Kentaro Taniguchi Shinji Uemoto Department of Transplant Surgery Nagoya University Hospital Department of Intensive Care Unit Nagoya University Hospital Second Department of Surgery Nagoya University Hospital Department of Transplant Surgery Kyoto University Hospital First Department of Surgery Mie University Hospital

Advanced liver cirrhosis is usually accompanied by portal hypertension. Long-term portal hypertension results in various vascular alterations. The systemic hemodynamic state in patients with cirrhosis is termed a hyperdynamic state. This peculiar hemodynamic state is characterized by an expanded blood volume, high cardiac output, and low total peripheral resistance. Vascular alterations do not disappear even long after liver transplantation(LT), and recipients with cirrhosis exhibit a persistent systemic hyperdynamic state even after LT. Stability of optimal systemic hemodynamics is indispensable for adequate portal venous flow(PVF) and successful LT, and reliable parameters for optimal systemic hemodynamics and adequate PVF are required. Even a subtle disorder in systemic hemodynamics is precisely indicated by the balance between cardiac output and blood volume. The indocyanine green(ICG) kinetics reflect the patient's functional hepatocytes and effective PVF, and PVF is a major determinant of the ICG elimination constant(kICG) in the well-preserved allograft. The kICG value is useful to set the optimal PVF during living-donor LT and to evaluate adequate PVF after LT. Perioperative management has a large influence on the postoperative course and outcome; therefore, key points and unexpected pitfalls for intensive management are herein summarized. Transplant physicians should fully understand the peculiar systemic hemodynamic behavior in LT recipients with cirrhosis and recognize the critical importance of PVF after LT.

关键词： Liver cirrhosis Portal hypertension Liver transplantation Indocyanine green Hyperdynamic

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Usefulness of the Guglielmi detachable coil for embolization of a systemic venous collateral after Fontan operation:A case report

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World Journal of Radiology 2012年第9期4卷 418-420页

作者： Tetsuo Sonomura Akira Ikoma Nobuyuki Kawai Tomohiro Suenaga takashi Takeuchi Hiroki Minamiguchi Shunji Uchita Motoki Nakai Hiroyuki suzuki Kazushi Kishi Morio Sato Department of Radiology Wakayama Medical University811-1 KimiideraWakayama 641-8510Japan Department of Pediatrics Wakayama Medical University811-1 KimiideraWakayama 641-8510Japan Department of Thoracic and Cardiovascular Surgery Wakayama Medical University811-1 KimiideraWakayama 641-8510Japan

Embolization of collateral veins is often treated with rigid coils(Gianturco and interlocking detachable coils type).However,when dealing with tortuous and dilated collateral veins,there is a high risk for technical failure and coil migration due to inflexibility of the *** safely and successfully solve this problem,Guglielmi detachable coils(GDC) can be used for *** flexibility allows for easy navigation in tortuous veins,low risk of unintended coil release or coil migration,and safe deployment.A 12-year-old girl with a single ventricle had severe cyanosis and a low exercise tolerance 5 years after Fontan *** symp-toms were caused by a tortuous and dilated collateral from the left phrenic vein into the left pulmonary vein,forming a right-to-left *** collateral,which had a large diameter and high flow,and therefore a high risk of coil migration,was successfully embolized with 8 *** were no complications such as coil migration or cerebral *** embolization increased her systemic oxygen saturation from 81%-84% to 94%-95%,and increased her ability to *** embolization procedure using flexible GDC was low risk compared with other rigid coil embolization techniques when performing embolization of tortuous and dilated collateral veins.

关键词： Pediatric intervention Embolization Systemic venous collateral Fontan operation Guglielmi detachable coil

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Bone markers predict survival in castration-resistant prostate cancer patients treated with docetaxel

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World Journal of Clinical Urology 2014年第2期3卷 139-143页

作者： Takumi Endo Naoto Kamiya Hiroyoshi suzuki Ryo Oka Fang-Ching Lee Takanobu Utsumi Masashi Yano Shuichi Kamijima Koji Kawamura takashi Imamoto Tomohiko Ichikawa Department of Urology Toho University Sakura Medical CenterSakura-shiChiba 285-8741Japan Department of Urology Graduate School of MedicineChiba UniversityChiba-shiChiba 260-8670Japan

AIM: To investigate the relationship between clinicopathological features and bone turnover markers in castration-resistant prostate cancer(CRPC) patients treated with ***: Thirty-three patients were enrolled in this study. Serum levels of carboxyterminal cross-linked telopeptide of type 1 collagen generated by metalloproteinases(1CTP) and alkaline phosphatase(ALP) were measured at the start of docetaxel chemotherapy. We examined the relationship between clinicopathological features and serum levels of 1CTP and ALP levels in CRPC patients treated with ***: For the total patient group, the mean ± standard deviation(SD) values for docetaxel chemotherapy dose, dose intensity, dosage interval, and num-ber of cycles were 59.3 ± 10.6 mg/m2, 13.9 ± 5.2 mg/m2 per week, 4.7 ± 1.2 wk, and 11.2 ± 7.4, respectively. Fourteen patients died from prostate cancer. Patients were divided into two groups according to mean + SD of serum 1CTP(8.2 ng/m L) and ALP(538.2 IU/L) levels at the start of docetaxel chemotherapy. Patients with lower levels of serum 1CTP and ALP had significantly better survivals than those with higher serum levels(P < 0.05).CONCLUSION: Serum levels of 1CTP and ALP are predictors of survival in patients with CRPC who are treated with docetaxel.

关键词： Prostate cancer Docetaxel chemotherapy Carboxy-terminal pyridinoline cross-linked telopeptide parts of type-1 collagen Alkaline phosphatase Prognostic factor

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