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journal of Forestry Research 2015年第3期26卷 711-718页

作者： Marcelo Luiz Laia Acelino Couto Alfenas Sergio Hermínio Brommonschenkel Shinitiro Oda Eduardo josé de Melo Inaê Mariêde araú jo Silva Janaína Fernandes Gonalves Ariadne Marques departamento de Engenharia Florestal Universidade Federal dos Vales do Jequitinhonha e Mucuri DiamantinaMinas Gerais 39100-000 Brazil departamento de Fitopatologia Universidade Federal de Viosa Viosa Minas Gerais 36570-900 Brazil Suzano Bahia Sul Celulose S/A Suzano So Paulo Brazil

Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resistant analogs （RGAs） from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previ- ously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the con- served domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting ana- lysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 （Ppr1） in a population study.

关键词： Cloning Conserved domains RGAs Rust Eucalyptus

Studying Formability Limits By Combining Conventional and Incremental Sheet Forming Process

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Chinese journal of Mechanical Engineering 2021年第4期34卷 78-89页

作者： Fabio Andre Lora Daniel Fritzen Ricardo Alves de sousa Lirio Schaffer department of Materials Engineering Federal University of Recôncavo da BahiaCampus CETENSFeira de SantanaBahia 44085-132Brazil SATC Uni-versity CriciumaBrazil Center of Mechanical Technology and Automation Department of Mechanical EngineeringUniversity of Aveiro3810-193 AveiroPortugal Federal University of Rio Grande do Sul Porto AlegreBrazil

In this work it is assessed the potential of combining conventional and incremental sheet forming processes in a same sheet of *** so-called hybrid forming approach is performed through the manufacture of a pre-forming by conventional forming,followed by incremental sheet *** main objective is analyzing strain *** pre-forming induced in the conventional forming stage will determine the strain paths,directly influencing the strains produced by the incremental *** conduct the study,in the conventional processes,strains were imposed in three different ways with distinct true *** the incremental stage,the pyramid strategy was adopted with different wall *** the experiments,the true strains and the final geometries were *** simulation was also employed for the sake of comparison and correlation with the measured *** could be observed that single-stretch pre-strain was directly proportional to the maximum incremental strains achieved,whereas samples subjected to biaxial pre-strain influenced the formability according to the degree of pre-strain ***-strain driven by the prior deep-drawing operation did not result,in this particular geometry,in increased formability.

关键词： Hybrid forming process Incremental sheet forming Strain paths Numerical simulation

Dynamics in the emergence of dormant and non-dormant herbaceous species from the soil seed bank from a Brazilian dry forest

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journal of Plant Ecology 2020年第3期13卷 256-265页

作者： josé Djalma de Souza Bruno Ayron de Souza Aguiar Danielle Melo dos Santos Vanessa Kelly Rodrigues de araujo Júlia Arruda Simões Juliana Ramos de Andrade Elcida de Lima araújo departamento de Biologia Universidade Federal Rural de PernambucoRecifePernambucoBrazil2Universidade Federal de Pernambuco-Centro Acadêmico de VitóriaPernambucoBrazil

Aims In dry tropical forests,herbaceous species may have dormancy mechanisms and form persistent and transient seed banks in the *** acquired,these mechanisms are efficient for the establishment and survival of these herbs,especially in forests with unpredictable climates,such as the ***,our objective was to verify whether the studied herbaceous species adopt the physical dormancy mechanism and how these natural barriers are overcome,to understand the temporal dynamics existing in the soil seed bank from a Brazilian dry tropical *** Seeds of five native herbaceous species from the Caatinga forests were selected and submitted to pre-germinative treatments for verifying the presence of physical *** collected soil samples in the rainy and dry seasons for four consecutive years and monitored the emergence of the selected herbaceous in the *** verified the differences in germination and seed bank emergence in the soil by generalized linear *** Findings The presence and absence of physical dormancy were observed in seeds from Caatinga herbaceous *** found intraspecific and interspecific differences in the herbaceous emergence from soil seed banks between years and climatic *** perennial herbs,consecutive lack of emergence between seasons and years was frequent,which suggests a direct relationship with the mechanism of physical dormancy and the environmental conditions necessary to overcome integument *** these species,seed dimorphism and dormancy may confer additional advantages to their ***,presenting intermediate levels of physical dormancy in an annual species may be an evolutionary adjustment to rainfall *** contrast,we found that the annual herb without dormancy is more sensitive to seasonal and interannual climate changes,as evidenced by the increase and significant reduction of its emergence in the soil seed *** differences acquired evoluti

关键词： establishment herbaceous species physical dormancy seed bank seed germination semiarid

Protein profile of leprosy patients with plantar ulcers from the Eastern Amazon region

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Infectious Diseases of Poverty 2017年第1期6卷 947-954页

作者： Marineia Porto de Oliveira jorge Rodrigues de sousa Rafael Silva de araujo Tinara Leila de sousa Aarao Juarez Antonio Simoes Quaresma Nucleo de Medicina Tropical Universidade Federal do ParaAv.Generalissimo Deodoro 92UmarizalBelemPara 66055240Brazil Centro de Ciencias Biologicas e da Saude Universidade do Estado do ParaBelemParaBrazil

Background:Studies investigating the nutritional status of patients with leprosy and plantar ulcers are ***,the objective of this study was to describe the protein profile of leprosy patients with plantar ulcers from the Eastern Amazon ***:A case record form was created for 75 patients with leprosy(31 with plantar ulcers and 44 without plantar ulcers)with the following data:sociodemographic characteristics,clinical form of leprosy,presence or absence of plantar ulcers,and nutritional assessment using anthropometry consisting of the measurement of body mass index,arm circumference,arm muscle circumference,and triceps *** of blood albumin,transferrin,and C-reactive protein(CRP)were also *** regarding protein intake were obtained using a Food Frequency ***:Plantar ulcers occurred more frequently in male patients(67.7%),patients aged 40-49 years(mean±SD:47.3±8.0 years),and patients receiving 300 or 600 USD(71.0%).The mean weight and height of patients were 71.6±11.4 kg and 1.62±0.1 m,*** levels of CRP were detected in 51.6%of leprosy patients with plantar ulcers and only 9.1%of patients without plantar ulcers(Pdepletion of transferrin was observed in 14.3%of patients with paucibacillary leprosy and 44.3%of patients with multibacillary leprosy(P=0.0447).Most patients had normal levels of serum albumin(74.2%with plantar ulcers and 77.3%without plantar ulcers).Conclusions:Most leprosy patients with plantar ulcers have normal levels of serum albumin and transferrin and high CRP levels,which indicates the presence of an inflammatory *** findings suggest the need to monitor patients with leprosy to prevent the occurrence of plantar ulcers and to provide adequate treatment for patients with existing plantar ulcers.

关键词： Leprosy Plantar ulcer Nutritional status Anthropometric assessment Albumin Transferrin C-reactive protein

An Adaptive Load Stepping Algorithm for Path-dependent Problems Based on Estimated Convergence Rates

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Computer Modeling in Engineering & Sciences 2017年第3期113卷 325-342页

作者： M.T.C.araújo Fernandes C.O.Cardoso W.J.Mansur Civil Engineering department/COPPE/Federal University of Rio de Janeiro Ilha do FundaoP.B.6850621945-970 Rio de JaneiroRJBrazil Petrobras/Cenpes Av.Horácio Macedo950Ilha do Fundao21941-915 Rio de JaneiroRJBrazil Civil Engineering department/COPPE/Federal University of Rio de Janeiro Ilha do FundaoP.B.6850621945-970 Rio de JaneiroRJBrazil

A new adaptive(automatic)time stepping algorithm,called RCA(Rate of Convergence Algorithm)is *** new algorithm was applied in nonlinear finite element analysis of path-dependent *** step size is adjusted by monitoring the estimated convergence rate of the nonlinear iterative *** RCA algorithm is relatively simple to implement,robust and its performance is comparable to,and in some cases better than,the automatic load incrementaion algorithm existent in commercial *** about the convergence rate of nonlinear iterative processes,an estimation of the rate and a study of the parameters of the RCA algorithm are *** show the capacity of the algorithm to adjust the increment size,detailed discussions based on results for different limit load analyses are *** results obtained by RCA algorithm are compared with those by ABAQUS?,one of the most powerful nonlinear FEA(Finite Element Analysis)commercial software,in order to verify the capability of RCA algorithm to adjust the increment size along nonlinear analyses.

关键词： Rate of convergence nonlinear automatic load incrementation limit load

Optimizing power oscillations in an ellipsometric system

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Chinese Optics Letters 2018年第3期16卷 48-52页

作者： Manoel P. araújo Stefano de Leo Gabriel G. Maia Institute of Physics Cleb Wataghin State University of Campinas Campinas 13083-872 Brazil department of Applied Mathematics State University of Campinas Campinas 13083-250 Brazil

Ellipsometry is a powerful and well-established optical technique used in the characterization of materials. It works by combining the components of elliptically polarized light in order to draw information about the optical system. We propose an ellipsometric experimental set-up to study polarization interference in the total internal reflection regime for Gaussian laser beams. The relative phase between orthogonal states can be measured as a power oscillation of the optical beam transmitted through a dielectric block, and the orthogonal components are then mixed by a polarizer. We show under which conditions the plane wave analysis is valid, and when the power oscillation can be optimized to reproduce a full pattern of oscillation and to simulate quarter- and half-wave plates.

关键词： Optimizing power oscillations in an ellipsometric system

Phenological study of congeneric Myrcia species and Clethra scabra in wetland and drained habitats in a Montane Forest

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journal of Forestry Research 2021年第4期32卷 1419-1427页

作者： Vinícius Londe Jaqueline Alves Pereira Hildeberto Caldas de sousa Lisbon Portugal Ecology of Tropical Biomes Graduate Course Federal University of Ouro PretoOuro PretoMinas Gerais 35400-000Brazil department of Biodiversity Evolution and EnvironmentFederal University of Ouro PretoOuro PretoMinas Gerais 35400-000Brazil

Although important,phenological studies comparing congeneric species or the same species growing in different habitats are still scarce for the ***,we integrate phylogeny,ecology and biometeorology to verify whether the phenophases of congeneric species Myrcia laruotteana and Myrcia amazonica or Clethra scabra differ when their populations inhabit wetland and drained habitats and to determine what abiotic factors affect the vegetative and reproductive phenophases of these species in distinct habitat *** collected data on phenological events of 80 trees for 1 year in Itacolomi State Park,Brazil,and related them to abiotic local *** to our expectation,the phenophases of the congeneric species did not differ between habitats,but the reproductive phenophases of *** did and was greater in drained *** of *** were affected by the depth of the water table and maximum temperature in the wetland ***,precipitation,maximum temperature and relative humidity influenced Myrcia and Clethra in the drained *** differences between *** populations suggest that this species is phenotypically plastic and can present distinct phenophases depending on the habitat it *** the other hand,the congeneric Myrcia species may have similar phenophases in distinct habitats because of their shared similarities during their *** study provides a better understanding of the ecology of these species and their adaptations to different abiotic *** of this nature are important in a changing world and can inform strategies for adaptive management.

关键词： Abiotic factors Forest ecology Phenophases Soil types

A Theoretical Study of Binary and Ternary Hydride-Bonded Complexes N(Beh2)...X with N = 1 or 2 and X = K+or Ca+2

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Open journal of Physical Chemistry 2011年第3期1卷 131-140页

作者： Boaz Galdino de Oliveira Regiane araújo 不详

A theoretical study of hydride bonds formed between beryllium hydride and alkaline earth metal cations is presented. B3LYP/6-311++G(d,p) calculations were used for determining the optimized geometries of the BeH2...K+, BeH2...Ca+2, BeH2...K+...BeH2, and BeH2...Ca+2...BeH2 hydride-bonded complexes, where among them the first are binaries, whereas the last ones are ternaries with the calcium (Ca+2) and potassium (K+) ions mediating the interactions with the beryllium hydride (BeH2). A detailed structural analysis were performed, by which the yielded profiles are in good agreement with results of the infrared vibrational spectrum, mainly in regards to the existence of red-shifted modes followed by enlarged absorption intensity ratios of the B-H bonds of the binary complexes. The capability of either donating or accepting of protons among BeH2, K+, and Ca+2, is currently treated in conformity with Lewis’s acid/base theory, but is also interpreted through the application of the Quantum Theory of Atoms in Molecules (QTAIM), whose formalism consents in the molecular modeling of concentrations and depletions of charge density ruled by the Laplacian shapes, charge transference fluxes, as well as by the local virial theorem of the electronic density with quantification of the kinetic and potential energies along the bonds and interactions.

关键词： Hydride Bonds Vibrational Infrared Spectrum B3LYP QTAIM

Antibacterial activity and inhibition against Staphylococcus aureus NorA efflux pump by ferulic acid and its esterified derivatives

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Asian Pacific journal of Tropical Biomedicine 2021年第9期11卷 405-413页

作者： Patrícia Gonçalves Pinheiro Gilvandete Maria Pinheiro Santiago Francisco Erivaldo Freitas da Silva Ana Carolina Justino de araújo Cícera Rejane Tavares de Oliveira Priscilla Ramos Freitas Janaína Esmeraldo Rocha joséBezerra de araújo Neto Maria Milene Costa da Silva Saulo Relison Tintino Irwin Rose Alencar de Menezes Henrique Douglas Melo Coutinho joséGalberto Martins da Costa departamento de Química Biológica Programa de Pos-Graduação em Química BiológicaUniversidade Regional do Cariri–URCACrato–CEBrazil Programa de Pós-Graduação em Química Centro de CiênciasUniversidade Federal do Ceará–UFCFortaleza–CEBrazil

Objective:To evaluate the inhibitory activity of ferulic acid and four of its esterified derivatives(methyl,ethyl,propyl,and butyl)against resistance mechanisms in Staphylococcus aureus ***:Ferulic acid derivatives were obtained by esterification with methanol,ethanol,propanol,and butanol,and then characterized by hydrogen and carbon-13 nuclear magnetic resonance *** minimum inhibitory concentrations(MIC)of ferulic acid and its esterified derivatives,ethidium bromide,and norfloxacin were obtained using the microdilution test,while the efflux pump inhibition test was conducted by examining reduction in the MICs of norfloxacin and ethidium *** docking was also carried out using the Schrodinger Suite 2015 molecular modeling software.A three-dimensional model of NorA efflux pump was generated using *** best scoring model was used as a receptor for ligand-receptor ***:The methyl and butyl ester derivatives did not demonstrate significant antimicrobial ***,a significant synergic effect was evidenced when norfloxacin was combined with the ethyl and propyl esterified *** docking study demonstrated favorable energy of interaction between ferulate derivatives and NorA,and amino acid residues TYR57,TYR58,and LEU255 were present commonly in stabilizing all *** PCA analysis corroborated the docking hypothesis that the lipophilic character and hydrogen bond interactions were the most relevant characteristics involved with NorA *** pharmacokinetic parameters of ferulic acid derivatives showed good ADMET properties,demonstrating that they can be easily absorbed and have no effect or inhibit the cytochrome P450 enzyme complex,revealing their potential as drug ***:This study provides strong evidence that the molecular basis for this activity is potentially due to the NorA efflux pump.

关键词： Ferulic acid Esterified derivatives Efflux pump Staphylococcus aureus Resistance mechanisms

How does the brown mussel Perna perna respond to environmental pollution?A review on pollution biomarkers

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journal of Environmental Sciences 2022年第1期34卷 412-428页

作者： Fernanda Silva dos Santos Raquel A.F.Neves Mirian araújo Carlos Crapez Valéria Laneuville Teixeira Natascha Krepsky Fluminense Federal University(UFF) Institute of BiologyGraduate Program in Science and BiotechnologyMario Santos Braga Streets/n.CentroNiteróiRJ CEP 24.020-141Brazil Federal University of the State of Rio de Janeiro(UNIRIO) Institute of Biosciences(IBIO)Graduate Program in Neotropical Biodiversity(PPGBIO)Pasteur Avenue458.UrcaRio de JaneiroRJ CEP 22.290-255Brazil Fluminense Federal University(UFF) Institute of BiologyGraduate Program in Marine Biology and Coastal EnvironmentsMario Santos Braga Streets/n.CentroNiteróiRJ CEP 24.020-141Brazil Federal University of the State of Rio de Janeiro(UNIRIO) Institute of Biosciences(IBIO)Graduate Program in Ecotourism and ConservationPasteur Avenue458.UrcaRio de JaneiroRJ CEP 22.290-255Brazil

The brown mussel Perna perna(Linnaeus,1758)is a valuable resource for aquaculture in tropical and subtropical coastal *** presents desirable characteristics for biomonitoring,including being sessile,widely distributed and abundant,and is a filter-feeder able to accumulate several classes of pollutants(e.g.,metals,hydrocarbons,among others).Mussels’biological responses to pollution exposure can be measured as biomarkers,which include alterations ranging from molecular to physiological levels,to estimate the degree of environmental contamination and its effects on *** full review compiles two decades(2000–2020)of literature concerning biological effects on *** mussel caused by environmental pollutants(i.e.,metals,hydrocarbons,and emerging pollutants),considering environmental and farm-based *** markers related to mussels’oxidative status were efficient for the biomonitoring of metals(i.e.,antioxidant enzymes associated with oxidative damage in biomolecules).Genotoxicity and cytotoxicity indicators(i.e.,comet,micronucleus,and neutral red assays)provided a depiction of hydrocarbon *** neutral red assay gave a time-concentration cytotoxic response to a wide range of pollutants,including emerging pollutants(e.g.,pharmaceuticals and biocides)and *** perna hemocyte parameters provided a useful approach for biocide *** paper summarizes useful biomarkers from molecular to physiological levels in this mussel species used to identify and quantify the degree of coastal *** integrated biomarker analysis may provide a way to overcome possible biomarker variations and assess multi-polluted ***,it is necessary to investigate biomarker variations according to natural factors(e.g.,season and gonad maturation stage)to standardize them for trustworthy biomonitoring.

关键词： Biological indexes Biomonitoring Emerging pollutants Hydrocarbons Metals Sentinel species