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Detection of KRAS G12D in colorectal cancer stool by droplet digital PCR

World Journal of Gastroenterology 2017年第39期23卷 7087-7097页

作者： susana olmedillas-lópez Dennis César lévano-linares Carmen laura Aúz Alexandre luz Vega-Clemente Edurne león Sánchez Alejandro Villagrasa Jaime Ruíz-Tovar Mariano García-Arranz Damián García-Olmo Foundation Health Research Institute-Fundación Jiménez Díaz University Hospital Department of Surgery School of Medicine Universidad Autónoma de Madrid Department of Surgery Rey Juan Carlos University Hospital Department of Pathology Fundación Jiménez Díaz University Hospital Department of Biomedicine and Biotechnology Universidad de Alcalá Department of Surgery Fundación Jiménez Díaz University Hospital

AIMTo assess KRAS G12D mutation detection by droplet digital PCR (ddPCR) in stool-derived DNA from colorectal cancer (CRC) *** this study, tumor tissue and stool samples were collected from 70 patients with stage I-IV CRC diagnosed by preoperative biopsy. KRAS mutational status was determined by pyrosequencing analysis of DNA obtained from formalin-fixed paraffin-embedded (FFPE) tumor tissues. The KRAS G12D mutation was then analyzed by ddPCR in FFPE tumors and stool-derived DNA from patients with this point mutation. Wild-type (WT) tumors, as determined by pyrosequencing, were included as controls; analysis of FFPE tissue and stool-derived DNA by ddPCR was performed for these patients as *** the total 70 patients included, KRAS mutations were detected by pyrosequencing in 32 (45.71%), whereas 38 (54.29%) had WT tumors. The frequency of KRAS mutations was higher in left-sided tumors (11 located in the right colon, 15 in the left, and 6 in the rectum). The predominant point mutation was KRAS G12D (14.29%, n = 10), which was more frequent in early-stage tumors (I-IIA, n = 7). In agreement with pyrosequencing results, the KRAS G12D mutation was detected by ddPCR in FFPE tumor-derived DNA, and only a residual number of mutated copies was found in WT controls. The KRAS G12D mutation was also detected in stool-derived DNA in 80% of all fecal samples from CRC patients with this point *** is a reliable and sensitive method to analyze KRAS G12D mutation in stool-derived DNA from CRC patients, especially at early stages. This non-invasive approach is potentially applicable to other relevant biomarkers for CRC management.

关键词： Droplet digital PCR KRAS Stool Formalin-fixed paraffin-embedded Pyrosequencing Colorectal cancer

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Secretome of polarized macrophages:potential for targeting inflammatory dynamics in spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3231-3232页

作者： Andreia Monteiro susana Monteiro Nuno A.Silva life and Health Sciences Research Institute(ICVS) School of MedicineUniversity of MinhoBragaPortugal ICVS/3B’s Associate lab PT Government Associated LabBraga/GuimaraesPortugal

Spinal cord injury(SCI)involves an initial traumatic phase,followed by secondary events such as ischemia,increased blood-spinal cord barrier permeability,ionic disruption,glutamate excitotoxicity,and metabolic alterations.A pe rsistent and exagge rated inflammato ry response within the spinal cord accompanies these events(lima et al.,2022).The complexity and interplay of these mechanisms exacerbate the initial injury,leading to a degenerative process at the injury *** the initial trauma is unavoidable,the secondary injury begins within minutes and can last for months,creating an optimal window for therapeutic intervention.

关键词： alterations injury companies

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The emerging role of mesenchymal stem cell-derived extracellular vesicles to ameliorate hippocampal NlRP3 inflammation induced by binge-like ethanol treatment in adolescence

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Neural Regeneration Research 2025年第4期20卷 1153-1163页

作者： susana Mellado María JoséMorillo-Bargues Carla Perpiñá-Clérigues Francisco García-García Victoria Moreno-Manzano Consuelo Guerri María Pascual Department of Physiology School of Medicine and DentistryUniversity of ValenciaValenciaSpain Bioinformatics and Biostatistics Unit Príncipe Felipe Research CenterValenciaSpain Neuronal and Tissue Regeneration laboratory Príncipe Felipe Research CenterValenciaSpain

Our previous studies have reported that activation of the NlRP3(NOD-,lRR-and pyrin domain-containing protein 3)-inflammasome complex in ethanol-treated astrocytes and chronic alcohol-fed mice could be associated with neuroinflammation and brain *** stem cell-derived extracellular vesicles(MSC-EVs)have been shown to restore the neuroinflammatory response,along with myelin and synaptic structural alterations in the prefrontal cortex,and alleviate cognitive and memory dysfunctions induced by binge-like ethanol treatment in adolescent *** the therapeutic role of the molecules contained in mesenchymal stem cell-derived extracellular vesicles,the present study analyzed whether the administration of mesenchymal stem cell-derived extracellular vesicles isolated from adipose tissue,which inhibited the activation of the NlRP3 inflammasome,was capable of reducing hippocampal neuroinflammation in adolescent mice treated with binge *** demonstrated that the administration of mesenchymal stem cell-derived extracellular vesicles ameliorated the activation of the hippocampal NlRP3 inflammasome complex and other NlRs inflammasomes(e.g.,pyrin domain-containing 1,caspase recruitment domain-containing 4,and absent in melanoma 2,as well as the alterations in inflammatory genes(interleukin-1β,interleukin-18,inducible nitric oxide synthase,nuclear factor-kappa B,monocyte chemoattractant protein-1,and C–X3–C motif chemokine ligand 1)and miRNAs(miR-21a-5p,miR-146a-5p,and miR-141-5p)induced by binge-like ethanol treatment in adolescent *** analysis further revealed the involvement of miR-21a-5p and miR-146a-5p with inflammatory target genes and NOD-like receptor signaling *** together,these findings provide novel evidence of the therapeutic potential of MSC-derived EVs to ameliorate the hippocampal neuroinflammatory response associated with NlRP3 inflammasome activation induced by binge drinking in adolescence.

关键词： adolescence binge-like ethanol treatment extracellular vesicles hippocampus mesenchymal stem cells neuroinflammation NOD-,lRR-and pyrin domain-containing protein 3(NlRP3)

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Efficacy of medium-chain fatty acid salts distilled from coconut oil against two enteric pathogen challenges in weanling piglets

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Journal of Animal Science and Biotechnology 2020年第1期11卷 251-267页

作者： Paola lópez-Colom lorena Castillejos Agustina Rodríguez-Sorrento Mónica Puyalto Juan JoséMallo susana María Martín-Orúe Animal Nutrition and Welfare Service Animal and Food Science DepartmentFacultat de VeterinàriaUniversitat Autònoma de BarcelonaBellaterraSpain Norel S.A MadridSpain.

Background:The search for alternatives to antibiotics in pig production has increased the interest in natural resources with antimicrobial properties,such as medium-chain fatty acids(MCFA)as in-feed *** study evaluated the potential of a novel blend of MCFA salts(DIC)from distilled coconut oil with a lauric acid content to reduce enteropathogens and control intestinal diseases around *** experimental disease models were implemented in early-weaned piglets,consisting of two oral challenges:Salmonella Typhimurium(1.2×10~8 CFU)or enterotoxigenic Escherichia coli(ETEC)F4(1.5×10~9 CFU).The parameters assessed were:animal performance,clinical signs,pathogen excretion,intestinal fermentation,immune-inflammatory response,and intestinal ***:The Salmonella challenge promoted an acute course of diarrhea,with most of the parameters responding to the challenge,whereas the ETEC F4 challenge promoted a mild clinical course.A consistent antipathogenic effect of DIC was observed in both trials in the hindgut,with reductions in Salmonella *** counts in the cecum(P=0.03)on d 8 post-inoculation(PI)(Salmonella trial),and of enterobacteria and total coliform counts in the ileum and colon(Pl).When analyzing the entire colonic microbiota(16 S rRNA gene sequencing),this additive tended(P=0.13)to reduce the Firmicutes/Bacteroidetes ratio and enriched Fibrobacteres after the Salmonella *** the ETEC F4 challenge,DIC prompted structural changes in the ecosystem with increases in Dialister,and a trend(P=0.14)to increase the Veillonellaceae *** parameters such as the intestinal fermentation products or serum pro-inflammatory mediators were not modified by DIC supplementation,nor were the histological *** the intraepithelial lymphocyte(IEl)counts were lowered by DIC in animals challenged with Salmonella(P=0.07).With ETEC F4,the IEl counts were higher with DIC on d 8 PI(P=0.08).Conclusions:This study c

关键词： Enteric pathogen Gut microbiota Intestinal immunity Medium-chain fatty acids Weaning pig

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Present and future management of viral hepatitis

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World Journal of Gastroenterology 2021年第47期27卷 8081-8102页

作者： Rocío González Grande Inmaculada Santaella leiva susana lópez Ortega Miguel Jiménez Pérez UGC de Aparato Digestivo.Unidad de Hepatología-Trasplante Hepático Hospital Regional Universitario de MálagaMálaga 29010Spain

Viral hepatitis can result in important morbidity and mortality,with its impact on health conditioned by the specific type of hepatitis,the geographical region of presentation and the development and access to new drugs,among other *** acute presentation forms are self-limiting and may even go unnoticed,with just a small percentage of cases leading to acute liver failure that may necessitate transplantation or even cause the death of the ***,when they become chronic,as in the case of hepatitis B virus and C virus,unless they are diagnosed and treated adequately they may have severe consequences,like cirrhosis or *** of the mechanisms of transmission,the pathogenesis,the presence of vaccinations and the development over recent years of new highly-efficient,potent drugs have meant that we are now faced with a new scenario in the management of viral hepatitis,particularly hepatitis B virus and hepatitis C *** spectacular advances in hepatitis C virus treatment have led the World Health Organization to propose the objective of its eradication by *** key aspect to achieving this goal is to ensure that these treatments reach all the more vulnerable population groups,in whom the different types of viral hepatitis have a high prevalence and constitute a niche that may perpetuate infection and hinder its ***,microelimination programs assume special relevance at the present time.

关键词： Hepatitis viral Diagnosis Treatment Trend direct-acting antivirals Inhibiting recycling

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Response of ants to grazing disturbance at the central Monte Desert of Argentina: community descriptors and functional group scheme

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Journal of Arid land 2014年第1期6卷 117-127页

作者： Silvia ClAVER susana l SIlNIK Florencia F CAMPóN Entomology lab IADIZA-CONICET

livestock ranching is one of the main productive activities in arid regions of the *** produces changes in animal as well as plant communities(***,abundance and species dominance relationships).Ants are good biological indicators due to the environmental fidelity of some of their community *** described the functional structure of the ant community in the central Monte of Mendoza,Argentina,and examined the effect of grazing using richness,diversity and the functional group *** used pitfall traps to sample ants at a reserve with 30-year cattle exclusion and at an adjacent *** of the 27 recorded species showed significant differences in their abundance and two species were absent at the *** richness and diversity did not reflect these differences,functional groups *** Climate Specialists were more abundant at the ranch while Cryptic Species and Generalized Myrmicinae increased at the *** study supports the utility of the functional group scheme to study the effects of grazing disturbance in ant communities of arid regions.

关键词： ants cattle grazing functional groups richness diversity arid zones Monte Desert

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Early psychological interventions for psychosis

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World Journal of Psychiatry 2015年第4期5卷 362-365页

作者： susana Ochoa Raquel lópez-Carrilero Research and Development Unit of Parc Sanitari Sant Joan de Déu Fundació Sant Joan de Déu CIBERSAM Sant Boi de Llobregat

The manuscript correspond to an editorial in order to assess the most important and effective interventions for people with psychosis in the early stages.

关键词： Schizophrenia Psychological interventions Psychosis Early psychosis Cognitive behavioral therapy

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Experimental modelling of flocculation processes-the case of Paraiba do Sul Estuary

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International Journal of Sediment Research 2014年第3期29卷 378-390页

作者： Alfredo TRENTO susana VINZON Department of Hydraulics National University of LitoralSanta FeArgentina Programa de Engenharia Oceanica(PEnO)/COPPE Federal University of Rio de JaneiroRio de JaneiroBrazil

The aggregation dynamics of fine sediments was analysed through laboratory tests using Couette and disk *** was shown that floc sizes tend to increase as concentrations grow both in fresh and salt water,in agreement with the aggregation theory,and that equilibrium diameters are slightly greater in salt environments for flocs developed either under shear stress or by differential *** transport and the aggregation processes were preliminarily studied in the estuary of the Paraiba do Sul River using a particle tracking model and field *** floc breakup process by shear stress was included in the *** stresses,which were determined by fractal dimensions and differential density,were accounted *** the calibration of the collision efficiency coefficients,the numerical model was able to predict floc sizes comparable with those measured at the Paraiba do Sul estuary,which,in turn,were similar to those obtained during the laboratory experiments in the Couette flocculator.

关键词： Flocs Flocculators Transport proceses Paraiba do Sul

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Indentation Size Effect inβCuAlBe and Cu-2Be Alloys

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Acta Metallurgica Sinica(English letters) 2021年第12期34卷 1669-1678页

作者： susana Montecinos Sebastian Tognana Walter Salgueiro IFIMAT Facultad de Ciencias ExactasUniversidad Nacional del Centro de La Provincia de Buenos AiresPinto 3997000 TandilArgentina Consejo Nacional de Investigaciones Científicas y Técnicas CONICET Av.Rivadavia 1917C1033AAJ Buenos AiresArgentina CIFICEN(UNCPBA-CICPBA-CONICET) Pinto 3997000 TandilArgentina Comisión de Investigaciones Científicas de la Provincia de Bs.As Calle 526 entre 10 y 111900 La PlataArgentina

The indentation size effect(ISE)was studied by instrumented nanoindentation and Vickers hardness measurements on polycrystalline copper,βCuAlBe pseudoelastic and age-hardenable CuBe *** in the load-depth curves at different loads would suggest a change in the behavior of the materials as the load *** was estimated at different loads taking into account the pile-up in each case,which was estimated from the images of the topography obtained by atomic force microscopy(AFM).The effect of the microstructure of Cu-2Be(wt%)samples on the ISE was also *** each copper alloy,the Nix and Gao model was applied to the hardness-depth curves,and significant differences were found for the parameters *** is in the constant nanohardness regime for depths below 500 nm,while microhardness exhibits the ISE *** behavior of the nanohardness and the plastic strain with the depth is almost *** presents the lowest values of plastic strains due to the pseudoelastic effect,while Cu presents an almost constant value around 8%,which corresponds to the highest plastic strain obtained.

关键词： Hardness Pseudoelasticity Copper alloys Plasticity Indentation size effect

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A STOPPING CRITERION FOR HIGHER-ORDER SWEEPING SCHEMES FOR STATIC HAMIlTON-JACOBI EQUATIONS

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Journal of Computational Mathematics 2010年第4期28卷 552-568页

作者： susana Serna Jianliang Qian Department of Mathematics University of California Los Angeles CA 90095 USA Department de Matematiques Universitat Autonoma de Barcelona 8193 Bellaterra Spain Department of Mathematics Michigan State University East Lansing MI 48824 USA

We propose an effective stopping criterion for higher-order fast sweeping schemes for static Hamilton-Jacobi equations based on ratios of three consecutive iterations. To design the new stopping criterion we analyze the convergence of the first-order lax-Friedrichs sweeping scheme by using the theory of nonlinear iteration. In addition, we propose a fifth-order Weighted PowerENO sweeping scheme for static Hamilton-Jacobi equations with convex Hamiltonians and present numerical examples that validate the effectiveness of the new stopping criterion.

关键词： Fast sweeping methods Gauss-Seidel iteration High order accuracy Static Hamilton-Jacobi equations Eikonal equations.

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