Arsenic contamination is a major environmental issue,as it may lead to serious health *** reduced trivalent formof inorganic arsenic,arsenite,is in generalmore toxic to plants comparedwith the fully oxidized pentavale...
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Arsenic contamination is a major environmental issue,as it may lead to serious health *** reduced trivalent formof inorganic arsenic,arsenite,is in generalmore toxic to plants comparedwith the fully oxidized pentavalent *** arsenite inplants hasbeenshown tobemediatedthrough a large subfamily of plant aquaglyceroporins,nodulin 26-like intrinsic proteins(NIPs).However,the efflux mechanisms,as well as themechanismof arsenite-induced root growth inhibition,remain poorly *** physiology,synchrotron imaging,and root transport assay approaches,we show that the cellular transport of trivalent arsenicals inArabidopsis thalianais stronglymodulatedbyPINFORMED2(PIN2)auxin efflux *** transport assay using radioactive arsenite,X-ray fluorescence imaging(XFI)coupled with X-ray absorption spectroscopy(XAS),and inductively coupled plasma mass spectrometry analysis revealed that pin2 plants accumulate higher concentrations of arsenite in roots comparedwith the *** the cellular level,arsenite specifically targets intracellular sorting of PIN2 and thereby alters the cellular auxin ***,loss of PIN2 function results in arsenite hypersensitivity in *** coupled with XAS further revealed that loss of PIN2 function results in specific accumulation of arsenical species,but not the other metals such as iron,zinc,or calcium in the root ***,these results suggest that PIN2 likely functions as an arsenite efflux transporter for the distribution of arsenical species in planta.
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