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残疾学生教育现状研究

三峡高教研究 2019年第2期37卷 30-34页

作者：孙晓锦罗晓华重庆三峡学院公共管理学院重庆万州404120

教育是一个民族发展的重要基础与保证。目前残疾人教育存在以下问题:特殊教育普及率不高;学前特殊教育与高等特殊教育发展缓慢,制约着残疾人的发展;地区之间特殊教育发展不均衡,拉大了地区间教育公平的距离。通过分析特殊教育现状,在此... 详细信息

教育是一个民族发展的重要基础与保证。目前残疾人教育存在以下问题:特殊教育普及率不高;学前特殊教育与高等特殊教育发展缓慢,制约着残疾人的发展;地区之间特殊教育发展不均衡,拉大了地区间教育公平的距离。通过分析特殊教育现状,在此基础上提出促进我国特殊教育发展的对策建议。

关键词：残疾儿童特殊教育教育公平

TATA-box binding protein-associated factor 2 regulates grain size in rice

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The Crop Journal 2023年第2期11卷 438-446页

作者： Ling Jiang Ning Jiang Zejun Hu Xuejun sun Xian Xiang Yahui Liu Mingwei Wu Chunming Liu xiaojin Luo Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology School of Life SciencesFudan UniversityShanghai 200438China School of Advanced Agricultural Sciences Peking UniversityBeijing 100871China

Grain size,characterized by a combination of grain length,width,and thickness,is one of the major determinants of yield in *** present study identified TATA-box binding protein-associated factor 2(TAF2)as an essential component regulating transcription and determining grain size in ***-based cloning showed that a G/T substitution in TAF2 resulted in a naturally occurring mutant called reduced grain size and plant height 1(rgh1).The mutants,with weak edited rgh1 alleles,exhibited a small grain phenotype with reduced grain length and width,while the severe knockout mutant(rgh1-2s)was dwarf and completely *** test performed between rgh1 and several edited alleles confirmed that the mutation in TAF2 caused the rgh1 *** staining showed that TAF2 was mainly expressed in the vascular bundles of roots,stems,leaves,and *** cytological analysis revealed that reduced cell division in the glumes resulted in the small grain phenotype of *** RNA-sequencing detected altered expression of genes involved in the basic biological processes in rgh1 *** findings provide novel insights into the TAF2-mediated genetic mechanism regulating grain size in rice.

关键词： Grain size TAF2 RGH1 Cell division Rice

siRNA沉默Smad4对奥沙利铂抑制乳腺癌MDA-MB-231细胞侵袭和迁移的影响

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山西医科大学学报 2015年第8期46卷 756-761页

作者：马琳艳宋乐乐黄莹莹孙小锦董淑英蒋志文刘浩蚌埠医学院药学系安徽省生化药物工程技术研究中心蚌埠233030 蚌埠医学院第一附属医院药剂科

目的探讨Smad4基因沉默对奥沙利铂(OXA)抑制乳腺癌细胞MDA-MB-231侵袭迁移能力的影响及其作用机制。方法采用MTT法检测不同浓度OXA(0,0.25,0.5,1,2,4μmol/L)对MDA-MB-231细胞的增殖抑制作用;Smad4 siRNA转染MDA-MB-231细胞株,并用Western blot检测转染后Smad4蛋白表达;Transwell小室法和细胞划痕实验检测MDA-MB-231细胞侵袭迁移活性;Western blot和ELISA检测细胞中TGF-β和MMP-9蛋白表达和活性的变化。结果 1μmol/L OXA作用于MDA-MB-231细胞24,48,72 h细胞存活率分别是(77.42±3.98)%,(60.38±3.65)%和(40.15±4.37)%。siRNA转染后MDA-MB-231细胞中Smad4的表达明显被抑制(P<0.05);Smad4 siRNA转染可增强OXA抑制MDA-MB-231细胞的侵袭和迁移活性(P<0.05);Smad4 siRNA转染使OXA下调细胞中TGF-β和MMP-9蛋白表达和活性明显增强(P<0.05)。结论下调Smad4的表达可以增强OXA抑制乳腺癌MDA-MB-231细胞侵袭迁移的活性,其机制可能与影响TGF-β/Smad4通路并下调MMP-9表达有关。

关键词：乳腺癌 Smad4 小干扰RNA 奥沙利铂侵袭迁移

Physical mechanism of performance adjustment in selective buried oxide n-MOSFETs

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Science China(Information Sciences) 2019年第6期62卷 186-188页

作者： Qin HUANG Renhua LIU Yabin sun xiaojin LI Yanling SHI Changfeng WANG DuANDuan LIAO Ming TIAN Key Laboratory of Multidimensional Information Processing Department of Electrical EngineeringEast China Normal University

Dear editor,FDSOI MOSFETs do not rely on channel doping to control short channel effects; they overcome the barriers of conventional bulk MOSFETs by means of scaling. Owing to the presence of the buried oxide insulato... 详细信息

关键词： MOS SOI Physical mechanism of performance adjustment in selective buried oxide n-MOSFETs

Additive and Over-dominant Effects Resulting from Epistatic Loci Are the Primary Genetic Basis of Heterosis in Rice

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Journal of Integrative Plant Biology 2009年第4期51卷 393-408页

作者： xiaojin Luo Yongcai Fu Peijiang Zhang Shuang Wu Feng Tian Jiayong Liu Zuofeng Zhu Jinshui Yang Chuanqing sun Department of Plant Genetics and Breeding and State Key Laboratory of Plant Physiology and Biochemistry China Agricultural University Beijing 100193 China National Centre for Evaluation of Agricultural Wild Plant (Rice) Beijing 100193 China Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture Beijing 100193 China State Key Laboratory of Genetic Engineenng Morgan-Tan Intemational Center for Life Sciences School of Life Sciences Fudan University Shanghai 200433 China Institute of Rice Research Anhui Academy of Agricultural Sciences Hefei 230031 China

A set of 148 F9 recombinant inbred lines （RILs） was developed from the cross of an indica cultivar 93-11 and japonica cultivar DTI13, showing strong F1 heterosis. Subsequently, two backcross F1 （BCFI） populations were constructed by backcrossing these 148 RILs to two parents, 93-11 and DT713. These three related populations （281BCF1 lines, 148 RILs） were phenotyped for six yield-related traits in two locations. Significant inbreeding depression was detected in the population of RILS and a high level of heterosis was observed in the two BCF1 populations. A total of 42 main-effect quantitative trait loci （M-QTLs） and 109 epistatic effect QTL pairs （E-QTLs） were detected in the three related populations using the mixed model approach. By comparing the genetic effects of these QTLs detected in the RILs, BCF1 performance and mid-parental heterosis （HMp）, we found that, in both BCF1 populations, the QTLs detected could be classified into two predominant types： additive and over-dominant loci, which indicated that the additive and over-dominant effect were more important than complete or partially dominance for M-QTLs and E-QTLs. Further, we found that the E-QTLs detected collectively explained a larger portion of the total phenotypic variation than the M-QTLs in both RILs and BCF1 populations. All of these results suggest that additive and over-dominance resulting from epistatic loci might be the primary genetic basis of heterosis in rice.

关键词： additivity epistasis heterosis over-dominance quantitative trait locus.

Seedling defoliation of cereal crops increases peanut growth and yield in an intercropping system

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The Crop Journal 2022年第2期10卷 418-425页

作者： Bo Huang xiaojin Zou Huasen Xu Jiayi Xu Huiyu Liu Wentao sun Liang Gong Shiwei Niu Liangshan Feng Ning Yang Lizhen Zhang Zhanxiang sun College of Land and Environment Shenyang Agricultural UniversityShenyang 110866LiaoningChina Center of International Cooperation Service Ministry of Agriculture and Rural AffairsBeijing 100125China Institute of Plant Nutrient and Environmental Resources Liaoning Academy of Agricultural SciencesShenyang 110161LiaoningChina Beijing Key Laboratory of Biodiversity and Organic Farming College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China

Intercropping cereals and legumes is practiced widely in the world for improving yields and economic benefits. Shorter legume crops in intercropping are shaded by taller cereals, substantially reducing legume growth and yield. Reducing shade in intercropping by shortening the plant height of cereals by seedling defoliation has been proposed as a practical approach to increase crop yields and land productivity. A two-year field experiment was conducted to investigate the effect of defoliation of cereal crops at seedling stage on the growth and yield of peanut(Arachis hypogaea L.) intercropped with corn(Zea mays L.) or millet(Setaria italica L.). In comparison with non-defoliation controls, defoliation reduced final plant height by 29 cm on average for corn and 18 cm for millet. Photosynthetically active radiation on peanut in intercropping systems with corn or millet intercropping was respectively 27.0% and 22.8% higher than those in controls, significantly improving the light environment of intercropped peanut. Net photosynthetic rates of peanut were on average 25.5% higher in corn and peanut intercropping and 19.6% higher in millet and peanut intercropping than those in non-defoliation controls. Total biomass of intercropped peanut increased owing to increased root growth. Across two years, yield of peanut intercropped with corn was 27.7% and with millet 32.8% higher than those of controls. Defoliation of cereal crops did not affect corn yield but significantly decreased millet yield by 24.5%. Our results suggest that applying seedling defoliation in intercropped corn could increase peanut yield without compromising corn yield in an intercropping system.

关键词： Plant height Strip intercropping Corn Growth Root

A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice

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Journal of Integrative Plant Biology 2012年第12期54卷 979-990页

作者： Zejun Hu Haohua He Shiyong Zhang Fan sun Xiaoyun Xin Wenxiang Wang Xi Qian Jingshui Yang xiaojin Luo State Key Laboratory of Genetic Engineering Institute of GeneticsSchool of Life SciencesFudan University Ministry of Education Key Laboratory for Crop Physiology Ecology and Genetic Jiangxi Super Rice Engineering Technology Research Center Jiangxi Agricultural University Institute of Rice Research Shandong Academy of Agricultural Sciences

A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice （Oryza sativa L.） varieties. In this study, we generated an F2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 （PA64）, an elite indica small-grain cultivar. Using QTL analysis, 17 QTLs for five grain traits were detected on four different chromosomes. Eight of the QTLs were newly-identified in this study. In particular, qGL3-1, a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation, was fine-mapped to the 17 kb region of chromosome 3. A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL. A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution （C→A） at position 1092 in exon 10, resulting in replacement of Asp （D） in PA64 with Glu （E） in CW23 for the 364th amino acid. This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat. Genetic analysis of a near-isogenic line （NIL） for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect, and is suitable for use in molecular marker-assisted selection.

关键词： Grain shape QTL mapping QTL fine mapping rice serine/threonine protein phosphatase.

多房棘球蚴感染诱导肝窦内皮细胞发生毛细血管化的动态研究

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中国血吸虫病防治杂志 2024年第1期36卷 34-43页

作者：张仁杰谢俊卫繁娜莫筱瑾宋鹏蔡玉春卢艳孙家辉周魇林琳张颋陈木新中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)、传染病溯源预警与智能决策全国重点实验室、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、WHO热带病合作中心、科技部国家级热带病国际联合研究中心上海200025 海南热带病研究中心(国家热带病研究中心海南分中心) 海南海口571199 内蒙古大学生命科学学院广东省深圳市疾病预防控制中心广东深圳518073

目的观察泡型棘球蚴病发展过程中肝窦内皮细胞(liver sinusoidal endothelial cells,LSEC)毛细血管化现象及与肝纤维化的关系,为探索LSEC在泡型棘球蚴病所致肝损伤和肝纤维化发生和转归中的作用机制奠定基础。方法取40只C57BL/6小鼠(6~... 详细信息

目的观察泡型棘球蚴病发展过程中肝窦内皮细胞(liver sinusoidal endothelial cells,LSEC)毛细血管化现象及与肝纤维化的关系,为探索LSEC在泡型棘球蚴病所致肝损伤和肝纤维化发生和转归中的作用机制奠定基础。方法取40只C57BL/6小鼠(6~8周龄),随机分为对照组和感染1、2、4周组,每组各10只。感染1、2、4周组每只小鼠经肝脏直视穿刺注射接种2000个多房棘球蚴原头节,对照组用相同方法注射等量磷酸盐缓冲液。在感染后1、2、4周,取小鼠肝脏,经苏木精-伊红(hematoxylin-eosin,HE)染色后观察肝脏病理变化,经Masson三色染色后对染色阳性区域面积进行半定量分析以判断肝纤维化程度,采用免疫组织化学染色法评价α-平滑肌动蛋白(α-smooth muscle actin,α-SMA)和Ⅰ型胶原蛋白α1(collagen typeⅠalpha 1,COL1A1)表达水平以判断肝星状细胞(hepatic stellate cell,HSC)活化和细胞外基质(extracellular matrix,ECM)沉积,用扫描电子显微镜观察LSEC表面窗孔。从小鼠肝脏中分离原代LSEC,用实时荧光定量PCR(real-time fluorescence quantitative PCR,qPCR)法检测LSEC标志基因稳定素(Stabilin)-1、Stabilin-2、Ehd3、CD209b、GATA4、Maf mRNA表达水平。结果多房棘球蚴感染2周后,可见小鼠肝脏局部肝小叶结构被破坏;感染4周后,小鼠肝脏可见多房棘球蚴囊泡,周围有肉芽肿组织包绕。Masson三色染色半定量分析结果显示,4组小鼠肝脏内胶原纤维比例差异有统计学意义(F=26.060,P<0.001),感染4周组小鼠肝脏内胶原纤维比例[(11.29±2.58)%]较对照组增加(P<0.001)。免疫组织化学染色结果显示,感染1、2周组小鼠肝脏内见少量HSC活化和ECM沉积;感染4周组小鼠肝组织中可见大量棕黄色α-SMA和COL1A1聚集沉积在病变区域,并向周围扩散。半定量分析结果显示,4组小鼠肝脏内α-SMA和COL1A1表达水平差异均有统计学意义(F=7.667、6.530,P均<0.05),感染4周组小鼠肝脏内α-SMA[(7.13±3.68)%]和COL1A1[(13.18±7.20)%]表达水平均较对照组增加(P均<0.05)。扫描电子显微镜结果显示,4组小鼠LSEC开窗频率和孔隙率差异均有统计学意义(F=37.730、16.010,P均<0.001),感染1[分别为(1.22±0.48)个/μm^(2)、(3.05±0.91)%]、2周组[分别为(3.47±0.10)个/μm^(2)、(7.57±0.23)%]小鼠LSEC开窗频率和孔隙率均较对照组降低(P均<0.001)。4组小鼠LSEC平均窗孔直径差异有统计学意义(F=15.330,P<0.001),感染1[(180.80±16.42)nm]、2周组[(161.70±3.85)nm]小鼠LSEC平均窗孔直径均较对照组增加(P均<0.05)。4组小鼠LSEC中Stabilin-1、Stabilin-2、Ehd3、CD209b、GA TA4、Maf mRNA表达水平差异均有统计学意义(F=153.100、57.010、31.700、177.400、17.740、72.710,P均<0.001);与对照组相比,感染1、2、4周组小鼠上述基因相对表达量均降低(P均<0.001)。结论多房棘球蚴感染可诱导小鼠LSEC发生毛细血管化,并使LSEC功能和表型标志基因表达水平降低;LSEC毛细血管化早于HSC活化及肝纤维化发生。

关键词：多房棘球蚴泡型棘球蚴病肝窦内皮细胞毛细血管化肝纤维化

一例无腹部辅助切口经阴道取出标本的腹腔镜结肠次全切除手术治疗慢传输型便秘的病例报道(附视频)

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中华结直肠疾病电子杂志 2024年第1期13卷 83-88页

作者：王索张晓进许锋王俊侍新宇孙金兵何宋兵苏州大学附属常熟医院常熟市第一人民医院普外科215500 苏州大学附属第一医院普外科 215006

便秘是患者常见临床就诊原因之一,随着人们生活方式及饮食结构的转变,其发病率逐年提高。对于严格内科治疗无效的顽固性慢传输型便秘,手术是有效的治疗手段。寻求一种安全、有效并且兼顾微创和美容要求的手术方式显得尤为迫切,本文报道... 详细信息

便秘是患者常见临床就诊原因之一,随着人们生活方式及饮食结构的转变,其发病率逐年提高。对于严格内科治疗无效的顽固性慢传输型便秘,手术是有效的治疗手段。寻求一种安全、有效并且兼顾微创和美容要求的手术方式显得尤为迫切,本文报道1例苏州大学附属常熟医院开展的腹腔镜次全切除术结合经自然腔道取标本手术(NOSES)治疗慢传输型便秘的案例,并对该术式的可行性、优劣势以及技术细节进行讨论。

关键词：便秘慢传输型便秘腹腔镜结肠次全切除术经自然腔道取标本手术(NOSES)