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Early selection of bread wheat genotypes using morphological and photosynthetic attributes conferring drought tolerance

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Journal of Integrative Agriculture 2019年第11期18卷 2483-2491页

作者： Hafiz Ghulam Muhu-Din Ahmed Abdus Salam khan LI Ming-ju sultan habibullah khan Muhammad Kashif Department of Plant Breeding and Genetics University of Agriculture FaisalabadFaisalabad 38000Pakistan Institute of Agricultural Environment and Resources Yunnan Academy of Agricultural SciencesKunming 650205P.R.China Center of Agricultural Biotechnology and Biochemistry University of Agriculture FaisalabadFaisalabad 38000Pakistan

Genetic diversity is the base of any genetic improvement breeding program aimed at stress *** variability among breeding materials is of primary importance in the achievements of a good crop ***,105 wheat genotypes were screened against drought stress using factorial completely randomized design at seedling stage to determine the genetic diversity and traits association conferring drought *** of variances revealed that all the studied parameters differed significantly among all genotypes,indicating the significance genetic variability existed among all genotypes for studied *** 10 best performance genotypes G1,G6,G11,G16,G21,G26,G39,G44,G51,and G61 were screened as drought tolerant,while five lowest performance genotypes G3,G77,G91,G98,and G105 were screened as drought *** length,chlorophyll a,chlorophyll b,and carotenoid contents were significantly correlated among themselves which exhibited the importance of these indices for rainfed areas in future wheat breeding *** length exhibited non-significant and negative association with other studied traits,and its selection seems not to be a promising criteria for this germplasm for drought *** performance genotypes under drought stress conditions will be useful in future wheat breeding program and early selection will be effective for developing high yielding and drought tolerant wheat varieties.

关键词： drought photosynthesis chlorophyll wheat seedling carotenoid

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Pedigree and SSR Data Analysis Reveal Dominant Prevalence of Few Parents in Pedigrees of Pakistani Wheat Varieties

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American Journal of Molecular Biology 2015年第1期5卷 1-6页

作者： Muhammad Sajjad sultan habibullah khan Rizwana Maqbool Department of Environmental Sciences COMSATS Institute of Information Technology Vehari Pakistan Center for Agricultural Biochemistry and Biotechnology University of Agriculture Faisalabad Pakistan Department of Crop and Soil Sciences Washington State University Pullman USA

The international recognition of the importance of genetic diversity demands continuous estimation of genetic diversity of in hand population as test of its buffering capacity against all putative threats. Randomly selected Pakistani wheat varieties developed during 1965-1999 and 2000-2011 were evaluated on the basis of pedigree and SSR data. At 2nd and 3rd levels of pedigree, average occurrence of a parent per variety was 2.1 times. The dominating parents included BLUEBIRD, KALYANSONA and SIETE-CERROS-66, which were present in the pedigrees of 71.42%, 64.28%, and 58.57% varieties, respectively. The varieties INQLAB-91 and KIRAN-95 had the same pedigree and were genetically identical as revealed by SSR data. Similarly, varieties PAVON-76 and SOGHAT-90 also had the same parents in their pedigrees. This genetic similarity was also confirmed by SSR based cluster. The SSR based PC1 and PC2 showed narrow genetic diversity confirming the presence of few dominating parents. The results emphasize the inclusion of novel and genetically diverse parents in Pakistani wheat breeding programs to maintain broader genetic base of varieties/cultivars for buffering the effects of ever changing virulent pathogens and crop growth environments.

关键词： Genetic Diversity Pedigrees SSR Wheat

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Family and/or Friends? Gene Mapping at Crossroads

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American Journal of Plant Sciences 2014年第1期5卷 112-118页

作者： Muhammad Sajjad sultan habibullah khan Rashid Mehmood Rana Center of Agricultural Biochemistry and Biotechnology (CABB) University of Agriculture Faisalabad Pakistan Department of Plant Breeding and Genetics Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi Pakistan

Mapping gene(s) underlying a specific trait offers an opportunity to plant breeders to apply marker assisted selection. All gene mapping approaches except LD mapping use family based segregation populations developed by crossing two or more parents. These family based gene mapping approaches include simple interval mapping, composite interval mapping, multiple interval mapping and Bayesian mapping etc. Each approach has its own advantages and disadvantages based on type of population and underlying statistical model. Unlike family based approaches, LD mapping uses population of unrelated individuals which are like friends belonging to different family backgrounds. Relative pros and cons of family and friends based approaches make them complementary to each other. Family based approaches identify wide chromosomal region underlying the trait of interest with relatively lower markers density, and therefore, have low mapping resolution. Conversely, friends based LD mapping identifies chromosomal region of interest with higher resolution using higher marker density. The integration of family and friends based approaches addresses their respective pros and cons successfully to enhance mapping resolution for more valid application of marker assisted selection.

关键词： Family Based Mapping LD Mapping NAM

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Genome-wide association analysis for stripe rust resistance in spring wheat(Triticum aestivum L.) germplasm

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Journal of Integrative Agriculture 2020年第8期19卷 2035-2043页

作者： Sher MUHAMMAD Muhammad SAJJAD sultan habibullah khan Muhammad SHAHID Muhammad ZUBAIR Faisal Saeed AWAN Azeem iqbal khan Muhammad Salman MUBARAK Ayesha TAHIR Muhammad UMER Rumana KEYANI Muhammad InamAFZAL Irfan MANZOOR Javed Iqbal WATTOO Aziz-ur REHMAN Department of Bioinformatics and Biotechnology GC UniversityFaisalabad 38090Pakistan Department of Biosciences COMSATS University Islamabad(CUI)Islamabad 45550Pakistan Centre of Agricultural Biochemistry and Biotechnology(CABB) University of AgricultureFaisalabad 38000Pakistan Department of Plant Breeding and Genetics University of AgricultureFaisalabad 38000Pakistan Department of Biotechnology Faculty of Life SciencesUniversity of Central PunjabLahore 54000Pakistan Wheat Research Institute Ayub Agricultural Research Institute(AARI)Faisalabad 38950Pakistan

Stripe rust is a continuous threat to wheat crop all over the *** causes considerable yield losses in wheat crop every *** deployment of adult plant resistance(APR)genes in newly developing wheat cultivars is the most judicious strategy to combat this ***,we dissected the genetics underpinning stripe rust resistance in Pakistani wheat *** association panel of 94 spring wheat genotypes was phenotyped for two years to score the infestation of stripe rust on each accession and was scanned with 203 polymorphic *** on D’measure,linkage disequilibrium(LD)exhibited between loci distant up to 45 c ***-trait associations(MTAs)were determined using mixed linear model(MLM).Total 31 quantitative trait loci(QTLs)were observed on all 21 wheat *** QTLs were newly discovered as well as 19 QTLs and 35 previously reported Yr genes were validated in Pakistani wheat *** major QTLs were ***.2 AL and ***.3 BS(PVE,11.9%).Dissection of genes from the newly observed QTLs can provide new APR genes to improve genetic resources for APR resistance in wheat crop.

关键词： wheat Puccinia striiformis LD GWAS MTA PCoA

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Genome Editing Tools: Need of the Current Era

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American Journal of Molecular Biology 2019年第3期9卷 85-109页

作者： Sabin Aslam sultan habibullah khan Aftab Ahmed Abhaya M. Dandekar Center of Agricultural Biochemistry and Biotechnology University of Agriculture Faisalabad Pakistan Center of Advanced Studies in Agriculture and Food Security University of Agriculture Faisalabad Pakistan Department of Plant Sciences University of California Davis CA USA

Genome editing is considered as the most widely used approach of the present era. It had become a basic need of the current micro and molecular biological experiments. Gene engineering finds its widespread applications in medical, industry and agricultural sector. Unlike previous genetic engineering practices to insert or delete a part of genetic material at random place, genome editing allows the precise manipulation of DNA at a specific location. Zinc Finger Nucleases (ZFNs), Transcription Activator like Effector Nucleases (TALENs), Clustered Regularly Interspresed Short Palindromic repeats (CRISPR/Cas system) and meganucleases (recombinases) are the prime tools for editing an organism’s genome. Genome editing tools have an advantage to selectively delete or to integrate specific genes at specific loci. Use of recombinases for specifying site has further reduced time to integrate genes site specifically. Site specific gene stacking by the use of recombinases coupled with ZFNs, TALENs, or CRISPR/Cas genes have paved new pathways to target genes site specifically and to improve germplasm in lesser time than conventional breeding approaches.

关键词： CRISPR/Cas Gene Stacking Recombinases TALENs ZFNs

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The Tale of Cotton Plant: From Wild Type to Domestication, Leading to Its Improvement by Genetic Transformation

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American Journal of Molecular Biology 2020年第2期10卷 91-127页

作者： Sabin Aslam sultan habibullah khan Aftab Ahmed Abhaya M. Dandekar Center of Agricultural Biochemistry and Biotechnology University of Agriculture Faisalabad Pakistan Department of Plant Sciences University of California Davis CA USA Center of Advanced Studies in Agriculture and Food Security University of Agriculture Faisalabad Pakistan

Cotton is considered as a major cash crop of the world. It earns huge foreign exchange by its valuable products;fiber, lint, cotton seed oil, hull and a lot more. Being an important fiber crop, it earns huge foreign exchange by contributing to textile and seed oil industry. This review summarizes cotton biology, its diversity and domestication, genome assembly, constraints in its production and methods to improve cotton plant to fulfill the need of textile and oil industry. But cotton is facing enormous biotic and abiotic stresses with insect pests being most prominent. Massive destruction caused by insects needs to be controlled for maintaining fruitful cotton crop production. Conventional breeding approaches are limited to improving single trait and integrate stable genes within plant genome in approximately 7 - 8 years. Improved biotechnological procedures have paved new pathways to target genes specifically and improve cotton germplasm in lesser time than conventional breeding.

关键词： Cotton Colored Cotton Cotton Domestication Genetic Diversity Transformation

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Enhancing cotton resilience to challenging climates through genetic modifications

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Journal of Cotton Research 2024年第2期7卷 196-206页

作者： AHMED Ali Ijaz khan Azeem Iqbal NEGM Mohamed A.M. IQBAL Rida AZHAR Muhammad Tehseen khan sultan habibullah RANA Iqrar Ahmad Department of Plant Breeding and Genetics University of AgricultureFaisalabadFaisalabad 38000Pakistan Cotton Research Institute-Giza GizaEgypt Centre for Advanced Studies in Agriculture and Food Security University of AgricultureFaisalabadFaisalabad 38000Pakistan Centre of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabadFaisalabad 38000Pakistan School of Agriculture Sciences Zhengzhou UniversityZhengzhou 450000China

Cotton is one of the most important fiber crops that plays a vital role in the textile *** production has been unstable over the years due to climate change induced biotic stresses such as insects,diseases,and weeds,as well as abiotic stresses including drought,salinity,heat,and *** breeding methods have been used to breed climate resilient cotton,but it requires a considerable amount of time to enhance crop tolerance to insect pests and changing climatic conditions.A promising strategy for improving tolerance against these stresses is genetic *** review article discusses the role of genetic engineering in cotton *** essential concepts and techniques include genome editing via clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(CRISPR-Cas9),overexpression of target genes,downregulation using RNA interference(RNAi),and virus-induced gene silencing(VIGS).Notably,the Agrobacterium-mediated transformation has made significant contributions to using these techniques for obtaining stable transgenic plants.

关键词： Cotton Genome editing Drought Salinity Heat

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Kinetics of Acid Blue 1 Adsorption from Aqueous Solution by Carbonaceous Substrate Produced from Biotic Precursor

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Chinese Journal of Chemistry 2007年第5期25卷 596-604页

作者： FAZLULLAH, khan Bangash sultan, Alam IRSHAD, Ahmad Institute of Chemical Sciences University of Peshawar Peshawar 25120 Pakistan National Center of Excellence in Geology University of Peshawar Peshawar 25120 Pakistan

Adsorption of acid blue 1 from aqueous solution onto carbonaceous substrate produced from the wood of Paulownia tomentosa was investigated. The samples characterized by FTIR, SEM, EDS and XRD techniques, indicated that the surface functional groups like carboxyl, lactones or phenols and ethers have disappeared at high activation temperature （800 ℃） and as a result porous structure was developed that has a positive effect on the adsorption capacity. Bangham and parabolic diffusion models were applied to the kinetic adsorption data, which show that the adsorption of acid blue 1 was a diffusion controlled process. The reaction rate increased with the increase in temperatures of both the adsorption and activation. Thermodynamic parameters like △E^≠, △H^≠, △S^≠ and △G^≠ were calculated from the kinetic data. The negative values of △S^≠ reflected the decrease in the disorder of the system at the solid-solution interface during adsorption. Gibbs free energy （△G^≠）, representing the driving force for the affinity of dye for the carbon surface, increased with the increase in sample activation and the adsorption temperatures.

关键词： activated carbon chemical treatment thermal activation adsorption functional group XRD SEM EDS FTIR

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Experimental investigation of evaporative cooling systems for agricultural storage and livestock air-conditioning in Pakistan

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Building Simulation 2021年第3期14卷 617-631页

作者： Hafiz M.U.Raza Muhammad sultan Majid Bahrami Alamgir A.khan Department of Agricultural Engineering Bahauddin Zakariya UniversityBosan RoadMultan 60800Pakistan Laboratory for Alternative Energy Conversion(LAEC) School of Mechatronic Systems EngineeringSimon Fraser UniversitySurreyBCCanada Department of Agricultural Engineering Muhammad Nawaz Shareef University ofAgricultureMultan 60000Pakistan

Evaporative cooling(EC)is an ancient technique that is usually suitable for hot and dry climatic conditions due to the potential of water vapor *** this study,three kinds of evaporative cooling systems such as direct EC(DEC),indirect EC(IEC),and Maisotsenko cycle EC(MEC)were locally developed at *** performance of the systems was evaluated and compared for agricultural storage and livestock air-conditioning application in *** experiments were performed for climatic conditions of Multan city(Pakistan)and the data were collected for hourly and daily *** to the results,it was observed that the DEC system has the ability to reduce the temperature of ambient air to an average of 8.5℃.Whilst IEC and MEC systems were able to drop the temperature of ambient air to an average of 6.8℃and 8.9℃,*** per the results,the DEC system remained behind to provide desired conditions for livestock and agricultural product storage applications due to excessive *** the other hand,the IEC and MEC systems can achieve the desired conditions for livestock application,but could not provide feasible conditions for various fruits and vegetable *** study concludes that hybrid EC systems can be developed to provide desired conditions for a wide range of applications under varying climatic conditions.

关键词： evaporative cooling air-conditioning dew-point wet-bulb storage livestock

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Mathematical modeling of mixed convective MHD Falkner-Skan squeezed Sutterby multiphase flow with non-Fourier heat flux theory and porosity

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Applied Mathematics and Mechanics(English Edition) 2023年第11期44卷 2005-2018页

作者： Shuguang LI M.I.khan F.ALI S.S.ABDULLAEV S.SAADAOUI habibullah School of Computer Science and Technology Shandong Technology and Business UniversityYantai 264005Shandong ProvinceChina Department of Mechanics and Engineering Science Peking UniversityBeijing 100871China Department of Mechanical Engineering Lebanese American UniversityBeirut 11020Lebanon Department of Mathematical Sciences Federal Urdu University of ArtsSciences&TechnologyGulshan-e-IqbalKarachi 75300Pakistan Faculty of Chemical Engineering New Uzbekistan UniversityTashkent 100070Uzbekistan Department of Science and Innovation Tashkent State Pedagogical University named after NizamiTashkent 100070Uzbekistan Department of Physics Faculty of Science and ArtsMohayel AserKing Khalid UniversityAbha 61421Saudi Arabia State Key Laboratory of Turbulence and Complex System Collaborative Innovative Center for Advanced Aero-EnginesPeking UniversityBeijing 100871China

In a wide variety of mechanical and industrial applications,e.g.,space cooling,nuclear reactor cooling,medicinal utilizations(magnetic drug targeting),energy generation,and heat conduction in tissues,the heat transfer phenomenon is ***’s law of heat conduction has been used as the foundation for predicting the heat transfer behavior in a variety of real-world *** model’s production of a parabolic energy expression,which means that an initial disturbance would immediately affect the system under investigation,is one of its main ***,numerous researchers worked on such problem to resolve this *** last,this problem was resolved by Cattaneo by adding relaxation time for heat flux in Fourier’s law,which was defined as the time required to establish steady heat conduction once a temperature gradient is *** offered a material invariant version of Cattaneo’s model by taking into account the upper-connected derivative of the Oldroyd ***,both models are combinedly known as the Cattaneo-Christov(CC)*** this attempt,the mixed convective MHD Falkner-Skan Sutterby nanofluid flow is addressed towards a wedge surface in the presence of the variable external magnetic *** CC model is incorporated instead of Fourier’s law for the examination of heat transfer features in the energy expression.A two-phase nanofluid model is utilized for the implementation of *** nonlinear system of equations is tackled through the bvp4c technique in the MATLAB software *** influence of pertinent flow parameters is discussed and displayed through different *** and important results are summarized in the conclusion ***,in both cases of wall-through flow(i.e.,suction and injection effects),the porosity parameters increase the flow speed,and decrease the heat transport and the influence of drag forces.

关键词： sporosity Cattaneo-Christov(CC)heat flux model Falkner-Skan Sutterby nanofluid mixed convection stagnation point

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