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Biophysical mechanism-mediated time-dependent effect on sperm of human and monk.y vas implanted polyelectrolyte contraceptive

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Asian Journal of Andrology 2007年第2期9卷 221-227页

作者： sujoy k. guha School of Medical Science and Technology Indian Institute of Technology and National Institute of Medical Science and Technology Kharagpur 721302 India

Aim： To determine the short and long-term morphological effects on sperm as induced by intra-vas alteration of pH and electrical charge. Methods： Desired biophysical influences were obtained by injection of reversible inhibition of sperm under guidance （RISUG） into the lumen of the vas deferens of human subjects and the monk.y. RISUG is a polyelectrolyte hydrogel complex of styrene maleic anhydride （SMA） and dimethyl sulfoxide （DMSO） which generates an electrostatic charge and also lowers in a near space of pH domain. The morphology of sperm was examined by light microscopy, scanning and transmission electron microscopy. Human study enabled semen collection by masturbation as early as 3 h after injection and studies extended up to 6 months, in the monk.y, on vas excision after RISUG implantation, sperm characteristics were examined in serial sections. Results： Semenology in clinical studies and histological data of the monk.y showed a time-sequenced sperm plasma membrane, tail mitochondria and nuclear decondensation alterations in sperm structural components, which beared mark.d similarity to changes in the sperm head and tail during capacitation and entry into the ovum. Conclusion： The findings provide a means of causing such changes in the sperm that inhibit the fertilizing ability before the nucleus is affected. Therefore achieving non-obstructive vas-based contraception, without genotoxic or teratogenic effects caused by infertile sperm passing into the semen, is feasible.

关键词： reversible inhibition of sperm under guidance vas deferens electrical charge sperm electron microscopy acrosome mitochondria chromatin decondensation

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Non-invasive reversal of intraluminal vas deferens polymer injection-induced azoospermia——technology

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Asian Journal of Andrology 1999年第3期1卷 131-134页

作者： sujoy k.guha Centre for Biomedical Engineering Indian Institute of Technology and All India Institute of Medical Sciences New Delhi-110016 India

Aim: The rationale and technique underlying a novel concept of non-invasive removal of an intravasal vas deferens poly-meric contraceptive drug to reverse drag injection-induced azoospermia are explained. Thus the conventional methods ofsurgical exploration to remove vas deferens plugs and intravasal injection of solvents to flush out contraceptive drugs are tobe replaced by steps which will be readily accepted by subjects. Methods: The approach is based upon the non-invasiveapplication of specific forces to various segments of the vas deferens so that non-sclerosing and non-tissue-adherent com-pounds, in particular styrene maleic anhydride (SMA) can be expelled. Forces are generated by palpation; percuta-neous electrical stimulation; vibration application; and percussion. The forces help to propel the intravasal polymer to-wards the ejaculatory duct for expulsion during ejaculation. All aspects of the total technique are clinically acceptable,simple, atraumatic, unlik.ly to cause pain and discomfort even without tranquilizers, local or general anaesthetics. Theprocedure may be repeated several times in different sittings spaced apart by about one week.to achieve adequate plug ex-pulsion. Results: Model experiments demonstrated the feasibility of the concept. The polymer was nonadherent andcould be moved within the vas deferens by the application of specific forces. Sufficient removal was possible to enablespermatic fluid to be transported along a region previously occupied by the polymer. A corroborating subhuman primatestudy by an independent investigator has shown that the semen profile becomes normal following the reversal. Conclu-sion: Adoption of the new technique may provide a means of non surgical restoration of normal semen profile after a pe-riod of fertility control obtained by intravasal drag injection. (Asian J Androl 1999 Sep ; 1: 131 - 134)

关键词： vas deferens male contraceptive agents maleic anhydrides sterilization reversal palpation electric stimulation

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