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Switches in transcriptome functions during seven skeletal muscle development stages from fetus to kid in Capra hircus

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Journal of Integrative Agriculture 2021年第1期20卷 212-226页

作者： ling Ying-hui ZHENG Qi JING Jing sui Meng-hua ZHU Lu LI Yun-sheng ZHANG Yunhai LIU Ya FANG Fu-gui ZHANG Xiao-rong College of Animal Science and Technology Anhui Agricultural UniversityHefei 230036P.R.China School of Natural and Environmental Sciences Newcastle UniversityNewcastle upon TyneNE17RUUK Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding Anhui Agricultural UniversityHefei 230036P.R.China

Skeletal muscle accounts for about 40% of mammalian body weight, the development of which is a dynamic, complex and precisely regulated process that is critical for meat production. We here described the transcriptome expression profile in 21 goat samples collected at 7 growth stages from fetus to kid, including fetal 45(F45), 65(F65), 90(F90), 120(F120), and 135(F135) days, and birth 1(B1) day and 90(B90) days kids. Paraffin sections combined with RNA-seq data of the 7 stages divided the transcriptomic functions of skeletal muscle into 4 states: before F90, F120, F135 and B1, and B90. And the dynamic expression of all 4 793 differentially expressed genes(DEGs) was identified. Furthermore, DEGs were clustered by weighted gene correlation network analysis into 4 modules(turquoise, grey, blue and brown) that corresponded to these 4 states. Functional and pathway analysis indicated that the active genes in the stages before F90(turquoise) were closely related to skeletal muscle proliferation. The DEGs in the F120-related module(grey) were found to participate in the regulation of skeletal muscle structure and skeletal muscle development by regulating t RNA. The brown module(F135 and B1) regulated fatty acid biological processes to maintain the normal development of muscle cells. The DEGs of B90 high correlation module(blue) were involved the strengthening and power of skeletal muscle through the regulation of actin filaments and tropomyosin. Our current data thus revealed the internal functional conversion of the goat skeletal muscle in the growth from fetus to kid. The results provided a theoretical basis for analyzing the involvement of m RNA in skeletal muscle development.

关键词： mRNA goat(Capra hircus) transcriptome skeletal muscle

Indirect comparison of efficacy and safety of chiglitazar and thiazolidinedione in patients with type 2 diabetes:A meta-analysis

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World Journal of Diabetes 2023年第10期14卷 1573-1584页

作者： Chu Lin Zong-Lin Li Xiao-ling Cai sui-Yuan Hu Fang Lv Wen-Jia Yang Li-Nong Ji Department of Endocrinology and Metabolism Peking University People's HospitalBeijing 100044China

BACKGROUND Chiglitazar is an emerging pan-agonist of all peroxisome proliferator activated receptors(PPAR)-α,δandγ,and has therapeutic potential for type 2 diabetes(T2D).However,to date,no clinical studies or meta-analyses have compared the efficacy and safety of chiglitazar and traditional PPAR-γagonist thiazolidinediones(TZDs).A meta-analysis concerning this topic is therefore *** To compare the efficacy and safety of chiglitazar and TZD in patients with *** PubMed,Medline,Embase,the Cochrane Central Register of Controlled Trials,Reference Citation Analysis and *** websites were searched from August 1994 to March *** controlled trials(RCTs)of chiglitazar or TZD vs placebo in patients with T2D were *** comparisons and sensitivity analyses were implemented to evaluate multiple efficacy and safety endpoints of *** We included 93 RCTs that compared TZD with placebo and one that compared chiglitazar with *** efficacy endpoints,the augmented dose of chiglitazar resulted in greater reductions in hemoglobin(Hb)A1c[weighted mean difference(WMD)=-0.15%,95%confidence interval(CI):-0.27 to-0.04%],triglycerides(WMD=-0.17 mmol/L,95%CI:-0.24 to-0.11 mmol/L)and alanine aminotransferase(WMD=-5.25 U/L,95%CI:-8.50 to-1.99 U/L),and a greater increase in homeostasis model assessment-β(HOMA-β)(WMD=17.75,95%CI:10.73-24.77)when compared with TZD *** safety endpoints,the risks of hypoglycemia,edema,bone fractures,upper respiratory tract infection,urinary tract infection,and weight gain were all comparable between the augmented dose of chiglitazar and *** patients with baseline HbA1c≥8.5%,body mass index≥30 kg/m^(2)or diabetes duration<10 years,the HbA1c reduction and HOMA-βincrease were more conspicuous for the augmented dose of chiglitazar compared with *** Augmented dose of chiglitazar,a pan-activator of PPARs,may serve as an antidiabetic agent with preferable glycemic and lipid control

关键词： Chiglitazar Thiazolidinedione Glycemic control β-cell function Drug safety

Design and fabrication of button-style beam position monitors for the HEPS synchrotron light facility

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Nuclear Science and Techniques 2022年第11期33卷 61-78页

作者： Jun He Yan-Feng sui Yong Li Xu-Hui Tang Lin Wang Fang Liu Zhi Liu ling-Da Yu Xiao-Yu Liu Tao-Guang Xu Jian-She Cao Jun-Hui Yue Key Laboratory of Particle Acceleration Physics and Technology Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China

At the High Energy Photon Source (HEPS),a high orbital stability of typically 10% of the beam size and angular divergence must be *** beam size at the insertion devices is 10μm horizontally and 1μm vertically,which implies that the beam orbit must be stabilized to the sub-micrometer *** results in stringent tolerance and quality control requirements for the series production of beam position monitor (BPM) *** this study,analytical formulas were used and CST simulations were performed to analyze the effects of the mechanical tolerances of BPM pickups on beam position *** results of electromagnetic?eld simulations revealed how various mechanical errors,such as button size and location accuracy,as well as the related button capacitance,exert different in?uences on the beam position *** performance of an actual BPM pickup was measured,along with an assessment of the error on the beam position ***,a wake?eld analysis,including an investigation of trapped resonant modes and related thermal deformation,was conducted.

关键词： High energy photon source BPM Error CST Tolerances

Shikani Optical Stylet–guided Intubation via the Intubating Laryngeal Airway in Patients With Scar Contracture of the Face and Neck

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Chinese Medical Sciences Journal 2013年第4期28卷 195-200页

作者： Dong Yang Shi-yi Tong Jin-hua Jin Geng-zhi Tang Jing-hu sui ling-xin Wei Xiao-ming Deng Department of Anesthesiology Plastic Surgery HospitalChinese Academy of Medical Sciences & Peking Union Medical College

Objective To evaluate the feasibility of the Shikani Optical Stylet （SOS）-guided inmbation through a new Intubafing Laryngeal Airway （ILA） in anticipated difficult airways caused by scar contracture of the face and neck. Methods Thirty-three adult patients with anticipated difficult airways undergoing selective faciocervical scar plastic surgery under general anesthesia were enrolled in this study. After anesthesia induction, a size 2.5, 3.5 or 4.5 ILA was inserted. Following good lung ventilation being verified, the SOS preloaded with an endotracheal tube was inserted via the ILA. Once the clear vocal cords came into view under the SOS, the endotracheal tube was advanced through glottis into the trachea. Results The ILA provided an effective airway in all patients, lntubation was successful at the first attempt on 22/33 （66.7%） occasions and at the second attempt on 6/33 （18.2%）. Intubation failed in 5 （15.1%） patients who suffered from severe limitation of head extension due to scar contracture of the neck. These patients＇ tracheas were finally intubated using a fibreoptic bronchoscope via the ILA. Conclusions The SOS-guided intubating method via the ILA is a feasible technique in patients with scar contracture of the face and neck. However, in patients with severe limitation of head extension, the use of SOS cannot be recommended. The SOS can be used as an alternative apparatus when the fibreoptic bronchoscope is not available.

关键词： Shikani Optical Stylet laryngeal mask airway difficult airway endotracheal intubation

Spinal CCL2 Promotes Pain Sensitization by Rapid Enhancement of NMDA-Induced Currents Through the ERK-GluN2B Pathway in Mouse Lamina Ⅱ Neurons

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Neuroscience Bulletin 2020年第11期36卷 1344-1354页

作者： Hui Zhang sui-Bin Ma Yong-Jing Gao Jun-ling Xing Hang Xian Zhen-Zhen Li Shu-Ning Shen Sheng-Xi Wu Ceng Luo Rou-Gang Xie Department of Neurobiology Fourth Military Medical UniversityXi'an 710032China Department of Health Statistics Fourth Military Medical UniversityXi'an 710032China Pain Research Laboratory Institute of Nautical MedicineJiangsu Key laboratory of NeuroregenerationNantong UniversityNantong 226001China Department of Radiation Biology Faculty of Preventive MedicineFourth Military Medical UniversityXi'an 710032China Department of Orthopedics Xijing HospitalFourth Military Medical UniversityXi'an 710032China Department of Stomatology No.984 Hospital of the People's Liberation ArmyBeijing 100094China

Previous studies have shown that CCL2(C-C motif chemokine ligand 2)induces chronic pain,but the exact mechanisms are still ***,we established models to explore the potential *** experiments revealed that an antagonist of extracellular signal-regulated kinase(ERK)inhibited not only CCL2-induced inflammatory pain,but also pain responses induced by complete Freund’s *** posed the question of the intracellular signaling cascade *** experiments showed that CCL2 up-regulated the expression of phosphorylated ERK(pERK)and N-methyl D-aspartate receptor[NMDAR]subtype 2B(GluN2B);meanwhile,antagonists of CCR2 and ERK effectively reversed these ***-cell patchclamp recordings revealed that CCL2 enhanced the NMDAR-induced currents via activating the pERK pathway,which was blocked by antagonists of GluN2B and *** summary,we demonstrate that CCL2 directly interacts with CCR2 to enhance NMDAR-induced currents,eventually leading to inflammatory pain mainly through the CCL2-CCR2-pERK-GluN2B pathway.

关键词： C-C motif chemokine ligand 2 Monocyte chemoattractant protein 1 Neuron-glial interaction Extracellular signal-regulated kinase

Robust generalized sidelobe canceller based on eigenanalysis and a MaxsinR beamformer

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Frontiers of Information Technology & Electronic Engineering 2019年第7期20卷 975-988页

作者： Quan-dong WANG Liang-hao GUO Wei-yu ZHANG sui-ling REN Chao YAN State Key Laboratory of Acoustics Institute of AcousticsChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100049China

A robust generalized sidelobe canceller is proposed to combat direction of arrival(DOA)*** estimate the interference-plus-noise(IPN)statistics characteristics,conventional signal of interest(SOI)extraction methods usually collect a large number of segments where only the IPN signal is *** avoid that collection procedure,we redesign the blocking matrix structure using an eigenanalysis method to reconstruct the IPN covariance matrix from the ***,a modified eigenanalysis reconstruction method based on the rank-one matrix assumption is proposed to achieve a higher reconstruction *** blocking matrix is obtained by incorporating the effective reconstruction into the maximum signal-to-interferenceplus-noise ratio(MaxsinR)*** can minimize the influence of signal leakage and maximize the IPN power for further noise and interference *** results show that the two proposed methods achieve considerable improvements in terms of the output waveform sinR and correlation coefficients with the desired signal in the presence of a DOA mismatch and a limited number of *** to the first proposed method,the modified one can reduce the signal distortion even further.

关键词： Eigenanalysis Interference-plus-noise covariance matrix reconstruction Maximum signal-to-interference-plus-noise ratio criterion Blocking matrix Generalized sidelobe canceller Direction of arrival mismatch

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) contributes to incorporation of histone variant H2A.Z into nucleosomes

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Protein & Cell 2019年第9期10卷 694-699页

作者： ling-Yao Wang Yun-xiao He Min Li Jian Ding Yi sui Joan W.Conaway Ronald C.Conaway Yong Cai Jingji Jin School of Life Sciences Jilin UniversityChangchun 130012 China Stowers Institute for Medical Research 1000E 50th StreetKansas CityMO 64110USA National Engineering Laboratory for AIDS Vaccine Jilin University Changchun 130012China Key Laboratory for Molecular Enzymology and Engineering The Ministry of EducationJilin UniversityChangchun 130012China

Current research has dem on strated that the dynamic distribution of histone variant H2A.Z at specific loci can be regulated by ATP-dependent chromatin remodelers, histone chaperones and histone post-translational mod... 详细信息

关键词： histone chromatin chaperones

Isolation and characterization of three pairs of verrucosidin epimers from the marine sediment‑derived fungus Penicillium cyclopium and confguration revision of penicyrone A and related analogues

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Marine Life Science & Technology 2023年第2期5卷 223-231页

作者： Yan-He Li Attila Mándi Hong-Lei Li Xiao-Ming Li Xin Li ling-Hong Meng sui-Qun Yang Xiao-Shan Shi Tibor Kurtán Bin-Gui Wang CAS and Shandong Province Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciencesand Laboratory of Marine Biology and Biotechnology at the Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China School of Marine Science University of Chinese Academy of SciencesBeijing 100049China Department of Organic Chemistry University of DebrecenEgyetem Tér 1Debrecen 4032Hungary Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Verrucosidins,a methylatedα-pyrone class of polyketides rarely reported upon,have been implicated in one or more neurological *** the signifcance of verrucosidins as neurotoxins,the absolute confgurations of most of the derivatives have not been accurately characterized *** this study,three pairs of C-9 epimeric verrucosidin derivatives,including the known compounds penicyrones A and B(1a/1b)and 9-O-methylpenicyrones A and B(2a/2b),the new compounds 9-O-ethylpenicyrones A and B(3a/3b),together with the related known derivative verrucosidin(4),were isolated and identifed from the culture extract of Penicillium cyclopium SD-413,which was obtained from the marine sediment collected from the East China *** structures were established based on an in-depth analysis of nuclear magnetic resonances(NMR)and mass spectroscopic *** of the absolute confgurations of these compounds was accomplished by Mosher’s method and time-dependent density functional theory(TDDFT)calculations of electronic circular dichroism(ECD)and optical rotation(OR).The confgurational assignment of penicyrone A demonstrated that the previously reported C-6 absolute confguration of verrucosidin derivatives needs to be revised from(6S)to(6R).The 9R/9S epimers of compounds 1–3 were found to exhibit growth inhibition against some pathogenic bacteria,indicating that they have potential as lead compounds for the creation of antimicrobial agents.

关键词： Marine-derived fungus Penicillium cyclopium Verrucosidin derivatives TDDFT-ECD calculations Mosher’s method Antimicrobial activity

Hydroxychavicol,a polyphenol from Piper betle leaf extract,induces cell cycle arrest and apoptosis in TP53-resistant HT-29 colon cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第2期22卷 112-122页

作者： Aiysvariyah RAJEDADRAM Kar Yong PIN sui Kiong ling See Wan YAN Mee Lee LOOI School of Biosciences Taylor’s UniversityLakeside Campus47500Subang JayaMalaysia Forest Research Institute Malaysia 52109KepongMalaysia Department of Biomedical Science Faculty of MedicineUniversity of Malaya50603Kuala LumpurMalaysia

This study aims to elucidate the antiproliferative mechanism of hydroxychavicol(HC).Its effects on cell cycle,apoptosis,and the expression of c-Jun N-terminal kinase(JNK)and P38 mitogen-activated protein kinase(MAPK)in HT-29 colon cancer cells were *** was isolated from Piper betle leaf(PBL)and verified by high-performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR),and gas chromatography-mass spectrometry(GC-MS).The cytotoxic effects of the standard drug 5-fluorouracil(5-FU),PBL water extract,and HC on HT-29 cells were measured after 24,48,and 72 h of *** cycle and apoptosis modulation by 5-FU and HC treatments were investigated up to 30 *** in phosphorylated JNK(pJNK)and P38(pP38)MAPK expression were observed up to 18 *** half maximal inhibitory concentration(IC_(50))values of HC(30μg/mL)and PBL water extract(380μg/mL)were achieved at 24 h,whereas the IC_(50)of 5-FU(50μmol/L)was obtained at 72 *** cycle arrest at the G0/G1 phase in HC-treated cells was observed from12 h *** apoptotic cell death in HC-treated cells compared to 5-FU-treated cells(P<0.05)was *** expression of pJNK and pP38 MAPK was observed at 12 h in HC-treated cells,but not in 5-FU-treated HT-29 cells(P<0.05).It is concluded that HC induces cell cycle arrest and apoptosis of HT-29 cells,with these actions possibly mediated by JNK and P38 MAPK.

关键词： Piper betle Hydroxychavicol(HC) Cell cycle Apoptosis c-Jun N-terminal kinase(JNK) P38 mitogen-activated protein kinase(MAPK)