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Effect of copper loading on texture,structure and catalytic performance of Cu/SiO_2 catalyst for hydrogenation of dimethyl oxalate to ethylene glycol

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Journal of Natural Gas Chemistry 2012年第5期21卷 563-570页

作者： Bo zhang Shengguo Hui suhua zhang Yang Ji Wei Li Dingye Fang State Key Laboratory of Chemical Engineering East China University of Science and TechnologyShanghai 200237China Pujing Chemical Industry(Sha)Limited Shanghai 200231China

Cu/SiO2 catalysts prepared by a convenient and efficient method using the urea hydrolysis deposition-precipitation （UHDP） technique have been proposed focusing on the effect of copper *** texture,structure and composition are systematically characterized by ICP,FTIR,N 2-physisorption,N2O chemisorption,TPR,XRD and *** formation of copper phyllosilicate is observed in Cu/SiO2 catalyst by adopting UHDP method,and the amount of copper phyllosilicate is related to copper *** is found the structure properties and catalytic performance is profoundly affected by the amount of copper *** excellent catalytic activity is attributed to the synergetic effect between Cu0 and Cu ＋.DMO conversion and EG selectivity are determined by the amount of Cu0 and Cu＋,*** proper copper loading （30 wt%） provides with the highest ratio of Cu ＋ /Cu0,giving rise to the highest EG yield of 95% under the reaction conditions of p=2.0 MPa,T=473 K,H2/DMO=80 and LHSV=1.0h-1.

关键词： Cu/SiO2 copper loading dimethyl oxalate ethylene glycol copper phyllosilicate

Investigation on the genetic-inconsistent paternity cases using the MiSeq FGx system

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Forensic Sciences Research 2022年第4期7卷 702-707页

作者： Anqi Chen Ruiyang Tao Chengtao Li suhua zhang Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina Department of Forensic Medicine School of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghaiChina

Mutations might challenge the paternity index calculation in forensic *** many studies have focussed on the autosomal short tandem repeats(A-STR),the mutation status of sex chromosomes and single nucleotide polymorphism(SNP)remain *** generation sequencing(NGS),known as high throughput and large sequence polymorphism,is a promising tool for forensic *** describe the mutation landscapes in the paternity cases with genetic inconsistencies,a total of 63 parentage confirmed paternity cases contained at least one mismatched locus have been *** mutations were subsequently evaluated using Verogen’s MPSForenSeqTM DNASignature Kit and a microsatellite instability(MSI)detection *** result showed 98.41%(62/63)of the cases had no additional autosomal mutations even when the number of A-STRs increased to *** for the sex chromosomes,about 11.11%(7/63)of the cases exhibited either X-STR or Y-STR mutations.D2S1338,FGAand Penta Ewere the most frequent altered STRs,which suggested they might be the mutation *** addition,a male with sex chromosome abnormality was observed accidently,whose genotype might be 47,XXY,rather than *** 56.90%of the STR loci possessed isoalleles,which might result in higher STR *** Mendelian incompatibility was detected among the SNP markers,which indicated that SNP was a more reliable genetic marker in the genetic-inconsistent paternity cases.

关键词： Forensic sciences forensic genetics paternity testing next generation sequencing(NGS) ForenSeqTM DNASignature Prep Kit

Detecting genetic hypermutability of gastrointestinal tumor by using a forensic STR kit

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Frontiers of Medicine 2020年第1期14卷 101-111页

作者： Anqi Chen suhua zhang Jixi Li Chaoneng Ji Jinzhong Chen Chengtao Li State Key Laboratory of Genetic Engineering Institute of GeneticsSchool of Life SciencesFudan UniversityShanghai 200433China Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformInstitute of Forensic SciencesMinistry of JusticeShanghai 200063China

Growing evidence suggests that somatic hypermutational status and programmed cell death-1 overexpression are potential predictive biomarkers indicating treatment benefits from immunotherapy using immune checkpoint ***,biomarker-matched trials are still limited,and many of the genomic alterations remain difficult to *** isolate the potential somatic hypermutational tumor from microsatellite instability low/microsatellite stability(MSI-L/MSS)cases,we employed two commercial kits to determine MSI and forensic short tandem repeat(STR)alternations in 250 gastrointestinal(GI)*** types of forensic STR alternations,namely,allelic loss,Aadd,and Anew,were identified.62.4%(156/250)of the patients with GI exhibited STR alternation,including 100%(15/15)and 60%(141/235)of the microsatellite high instability and MSI-L/MSS cases,respectively.30%(75/250)of the patients exhibited STR instability with more than 26.32%(26.32%–84.21%)STR *** cutoff with 26.32%of the STR alternations covered all 15 MSI cases and suggested that it might be a potential *** the similar mechanism of the mutations of MSI and forensic STR,the widely used forensic identifier STR kit might provide potential usage for identifying hypermutational status in GI cancers.

关键词： mismatch repair protein deficiency(MMR-D) microsatellite instability(MSI) short tandem repeats(STR) gastrointestinal tumor hypermutability

Mitochondrial genome sequencing with short overlapping amplicons on MiSeq FGx system

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Forensic Sciences Research 2022年第2期7卷 142-153页

作者： Yang Xin Rulin Jia suhua zhang Fei Guo Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina Forensic Science College Criminal Investigation Police University of ChinaShenyangChina

With the development and maturation of massively parallel sequencing(MPS)technology,the mitochondrial genome(mitogenome)sequencing is increasingly applied in the forensic *** this study,we employed the strategy of short overlapping amplicons for the whole mitogenome,library preparation with tagmentation using the Nextera®XT DNA Library Preparation Kit,sequencing on the MiSeq FGxTM Forensic Genomics System and analyzing data using the mitochondrial(mtDNA)MSR Plug-in and the mtDNA Variant Analyzer.A total of 27 libraries and 56 libraries were sequenced in a run using MiSeq Reagent Kit v2 and v3,*** showed more than 1800×of averaged depth of coverage(DoC)at each *** haplotypes of 9947 A and 2800 M were obtained at 32 ***-reactivity was observed with 1 ng primate DNA and 10 ng non-primate DNA but could be easily *** and accurate variants were obtained from at least 50 pg input DNA and from minor contributors between 19:1 and 1:19 mixed ratios with known reference *** than 86%variants were detected from≥200-bp degraded samples but its haplotype was assigned to more ancestral ***,a total of 3962 variants were observed at 613 nucleotide positions from 103 Xibe mitogenomes with 25:1 ratio of transitions to *** new transversions(C13735A and A14755C)and two tri-alleles at nps 9824 and 16092 were *** were 103 unique mitogenome haplotypes from 103 Chinese Xibe that were assigned to 79 *** D was the preponderant top-level haplogroup in Xibe followed by F,B,M,A,N,G,C,Z,Y,HV and *** match probability(RMP)and haplotype diversity(HD)of the whole mitogenome was calculated as 0.0097 and 1.0000,*** with HVS-I only,RMP decreased 33.56%,while the number of haplotypes and HD increased 15.73%and 0.49%,*** component analysis(PCA)showed that Xibe was clustered to East and Southeast *** a whole,this MPS strategy i

关键词： Forensic sciences massively parallel sequencing MiSeq FGx system mitochondrial genome Xibe degraded samples

Research progress of STC2 in breast cancer

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Biophysics Reports 2021年第3期7卷 185-192页

作者： Xuezhi Niu Yong Zhan suhua zhang Zixin Liu Chang Qu Key Laboratory of Hebei Province for Molecular Biophysics Institute of BiophysicsSchool of ScienceHebei University of Technology

Breast cancer ranks second in the list of most common cancers among women and brings the double burden of economy and health to women. Therefore, it is an urgent and necessary task to study the pathogenic mechanism and the treatment of breast cancer. Glycoprotein hormone is a kind of hormones to promote the growth and the development of cell and stanniocalcin 2(STC2) is one of *** has shown us a various expression of SCT2 in organs and tissues and it can regulate many different pathological and physiological processes. In addition, there are a lot of previous studies that indicated a close correlation between STC2 and the development and metastasis of many cancers, which infers STC2 can serve as biomarker of certain cancers. Until now, the effects of STC2 on breast cancer have been studied widely, but research findings demonstrated two different views, one view is that STC2 plays an oncogenic role and the other is the opposite. In this paper, it will summarize and evaluate the research data and results about mammalian STC2 on breast cancer.

关键词： Breast cancer Proliferation Metastasis Prognosis Invasion Stanniocalcin 2 (STC2)

Forensic parameters of 41 Y-STR loci in Shandong Han individuals and comparison with 42 other populations

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Forensic Sciences Research 2022年第4期7卷 823-825页

作者： Anqi Chen Li Luo Ruiyang Tao suhua zhang Chengtao Li Department of Forensic Medicine School of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghaiChina Shanghai Key Laboratory of Forensic MedicineShanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceMinistry of JusticeShanghaiChina

The Y-chromosomal short tandem repeat polymorphisms(Y-STRs)are the male-specific *** characteristics of paternal lineages make it a valuable tool for tracing familial relationships[1-3].Y-STR analysis has been widely used for identifying genealogical DNA testing and to identify missing persons,assess paternal relationships,and investigate sexual assault cases[4,5].

关键词： Forensic Shandong comparison

Validation of the Investigator 24plex QS Kit:a 6-dye multiplex PCR assay for forensic application in the Chinese Han population

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Forensic Sciences Research 2022年第2期7卷 172-180页

作者： Ruiyang Tao Chong Chen Xiang Sheng Ruocheng Xia Xiaochun zhang Jingyi zhang Zihao Yang suhua zhang Chengtao Li Institute of Forensic Medicine West China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengduChina Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic SciencesMinistry of JusticeShanghaiChina College of Medicine and Forensics Xi’an Jiaotong University Health Science CenterXi’anChina QIAGEN HildenGermany Department of Forensic Medicine School of Basic Medical ScienceWenzhou Medical UniversityWenzhouChina Department of Forensic Science Medical School of Soochow UniversitySuzhouChina

The Investigator 24plex QS Kit(QIAGEN,Hilden,Germany)is a 6-dye fluorescent chemistry short tandem repeat(STR)polymerase chain reaction(PCR)amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System(CODIS)core STR loci,SE33,DYS391,and the standard sex-determining locus,amelogenin,as well as two special internal performance quality sensor controls(QS1 and QS2),which are included in the primer mix to check the PCR *** study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions,sensitivity,precision,accuracy,repeatability,reproducibility,and concordance;tolerance to PCR inhibitors;applicability to real“forensic-type”samples;species specificity;mixture,balance and stutter analyses,and utility in a population *** exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate,sensitive and robust for STR *** addition,these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.

关键词： Forensic sciences forensic genetics short tandem repeat(STR)typing Investigator 24plex QS Kit validation study

Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups

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Forensic Sciences Research 2018年第2期3卷 138-144页

作者： Xiang Sheng Yali Wang Jiashuo zhang Liqin Chen Yuan Lin Zhenmin Zhao Chengtao Li suhua zhang Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformAcademy of Forensic ScienceShanghaiChina Department of Forensic Medicine Medical College of Soochow UniversitySuzhouChina Department of Forensic Medicine Inner Mongolia Medical UniversityHohhotChina

A forensic validation study of the Early Access HuaxiaTM Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and *** genotyping of DNA samples was consistent across a large range of template DNA concentrations,with complete profiles obtained at 0.125 ng;however,no more than 2 mm× 1.2 mm punches of samples would be recommended for direct *** size precision and accuracy test revealed the genotyping ability;while consistent results were obtained when comparing the kit with other commercially available *** addition,the whole PCR amplification can finish within approximately 45 min,making the system suitable for ***,only partial profiles may be obtained with challenging samples,including DNA stored on Foam-Tipped Applicators (FTA) cards or some case *** the forensic application in ethnic groups,a total of 282 and 229 alleles were obtained in Han and Mongolia,*** the 23 short tandem repeats were independent from each other,the cumulative power of exclusion in duos was 0.999999157188 and the cumulative power of exclusion in trios was 0.999999999859 in the Han group while the cumulative power of exclusion in duos (CPEduo) was 0.999 998 848 26 and cumulative power of exclusion in trios (CPEtrio) was 0.999 999 999 79 in the Mongolia *** good internal consistency was found between the two investigated groups and the Sichuan Han,Hui,Tibetan and Uygur according to available reference data.

关键词： Forensic genetics short tandem repeats(STRs) Early Access HuaxiaTM Platinum PCR kit

Massively parallel sequencing of 231 autosomal SNPs with a custom panel:a SNP typing assay developed for human identification with Ion Torrent PGM

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Forensic Sciences Research 2017年第1期2卷 26-33页

作者： suhua zhang Yingnan Bian Anqi Chen Hancheng Zheng Yuzhen Gao Yiping Hou Chengtao Li Shanghai Key Laboratory of Forensic Medicine Shanghai Forensic Service PlatformInstitute of Forensic SciencesMinistry of JusticePRCShanghaiChina State Key Laboratory of Genetic Engineering Institute of GeneticsSchool of Life SciencesFudan UniversityShanghaiChina Department of Forensic Medicine Medical College of Soochow UniversitySuzhouChina Department of Forensic Genetics West China School of Preclinical and Forensic MedicineSichuan UniversityChengduChina

The custom-designed single nucleotide polymorphism(SNP)panel amplified 231 autosomal SNPs in one PCR reaction and subsequently sequenced with massively parallel sequencing(MPS)technology and Ion Torrent personal genome machine(PGM).SNPs were chosen from SNPforID,IISNP,HapMap,dbSNP,and related published *** concordance was obtained between available MPS calling and Sanger sequencing with 9947A and 9948 *** SNPs(rs4606077,rs334355,rs430046,rs2920816,rs4530059,rs1478829,rs1498553,rs7141285,rs12714757 and rs2189011)with low coverage or heterozygote imbalance should be optimized or excluded from the *** data had sufficiently high coverage and gave reliable SNP calling for the remaining 221 loci with the custom MPS-SNP panel.A default DNA input amount of 10 ng per reaction was recommended by Ampliseq technology but sensitivity testing revealed positive results from as little as 1 ng input *** testing with this panel is possible through analysis of the F MAR(frequency of major allele reads)values at most loci with enough high coverage depth and low level of sequencing *** results indicate the potential advantage of the custom MPS-SNP assays and Ion Torrent PGM platform for forensic study.

关键词： Forensic science forensic genetics single nucleotide polymorphism(SNP) massively parallel sequencing(MPS) Ion Torrent personal genome machine(Ion Torrent PGM)