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Almond diversity and homozygosity define structure, kinship, inbreeding, and linkage disequilibrium in cultivated germplasm, and reveal genomic associations with nut and seed weight

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Horticulture Research 2021年第1期8卷 223-234页

作者： stefano pavan Chiara Delvento Rosa Mazzeo Francesca Ricciardi Pasquale Losciale Liliana Gaeta Nunzio D’Agostino Francesca Taranto Raquel Sanchez-Perez Luigi Ricciardi Concetta Lotti Department of Soil Plant and Food ScienceUniversity of Bari Aldo MoroVia Amendola 165/ABari 70126Italy Institute of Biomedical Technologies National Research Council(CNR)Via Amendola 122/DBari 70126Italy Department of the Sciences of Agriculture Food and EnvironmentUniversity of FoggiaVia Napoli 25Foggia 71100Italy Council for Agricultural Research and Economics-Research Centre for Agriculture and Environment(CREA-AA) Bari 70125Italy Department of Agricultural Sciences University of Naples Federico IIPortici 80055Italy Institute of Biosciences and Bioresources National Research Council of ItalyPortici 80055Italy CEBAS-CSIC.Campus Universitario de Espinardo 30100 EspinardoSpain

Almond[Prunus dulcis Miller(D.A.Webb)]is the main tree nut species worldwide.Here,genotyping-by-sequencing(GBS)was applied to 149 almond cultivars from the ex situ collections of the Italian Council for Agricultural Research(CREA)and the Spanish National Research Council(CSIC),leading to the detection of 93,119 single-nucleotide polymorphisms(SNPs).The study of population structure outlined four distinct genetic groups and highlighted diversification between the Mediterranean and Californian gene pools.Data on SNP diversity and runs of homozygosity(ROHs)allowed the definition of kinship,inbreeding,and linkage disequilibrium(LD)decay in almond cultivated germplasm.Four-year phenotypic observations,gathered on 98 cultivars of the CREA collection,were used to perform a genome-wide association study(GWAS)and,for the first time in a crop species,homozygosity mapping(HM),resulting in the identification of genomic associations with nut,shell,and seed weight.Both GWAS and HM suggested that loci controlling nut and seed weight are mostly independent.Overall,this study provides insights on the almond cultivation history and delivers information of major interest for almond genetics and breeding.In a broader perspective,our results encourage the use of ROHs in crop science to estimate inbreeding,choose parental combinations minimizing the risk of inbreeding depression,and identify genomic footprints of selection for specific traits.

关键词： linkage structure Italian

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Fertility preservation after bilateral severe testicular trauma

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Asian Journal of Andrology 2014年第4期16卷 650-651页

作者： Giovanni Liguori Nicola pavan Gianluca d'Aloia stefano Bucci Bernardino de Concilio Giorgio Mazzon Giangiacomo Ollandini Carlo Trombetta Department of Urology University of Trieste Cattinara Hospital Trieste Italy.

Dear Editor, Testicular trauma is a frequent acquired cause o fin fertility; being accidents, work injuries and sport activities that are the most common causes of testicular traumas.1＇2 Strangely, the issue of management of serious testicular trauma and fertility preservation has not been studied extensively before, existing only sporadic reports in international literature. Besides, no guidelines exist on fertility preservation in cases of important scrotal trauma when injury may result in irreparable damage to all testicular tissue （torsion of a solitary testis, bilateral synchronous testicular torsion,

关键词： CANCER SPERM

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