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Molecular Plant 2012年第1期5卷 115-130页

作者： stefanie Ranf Julia Grimmer Yvonne P6schl Pascal Pecher Delphine Chinchilla Dierk Scheel Justin Lee Leibniz Institute of Plant Biochemistry Stress and Developmental Biology Weinberg 3 D-06120 Halle Germanyb Present address： Institute of Biochemistry and Biotechnology Martin Luther University Halle-Wittenberg Weinbergweg 22 D-06120 Halle Germany Institute of Computer Science Martin Luther University Halle-Wittenberg Von-Seckendorff-Platz 1 D-06120 Halle Germany Zurich-Basel Plant Science Center Botanical Institute University of Basel Hebelstrasse 1 4056 Basel Switzerland

Calcium acts as a second messenger for signaling to a variety of stimuli including MAMPs （Microbe-Associated Molecular Patterns）, such as fig22 and elf18 that are derived from bacterial flagellin and elongation factor Tu, respectively. Here, Arabidopsis thaliana mutants with changed calcium elevation （cce） in response to fig22 treatment were isolated and characterized. Besides novel mutant alleles of the fig22 receptor, FLS2 （Flagellin-Sensitive 2）, and the receptor-associated kinase, BAK1 （Brassinosteroid receptor 1-Associated Kinase 1）, the new cce mutants can be categorized into two main groups--those with a reduced or an enhanced calcium elevation. Moreover, cce mutants from both groups show differ- ential phenotypes to different sets of MAMPs. Thus, these mutants will facilitate the discovery of novel components in early MAMP signaling and bridge the gaps in current knowledge of calcium signaling during plant-microbe interactions. Last but not least, the screening method is optimized for speed （covering 384 plants in 3 or 10 h） and can be adapted to genetically dissect any other stimuli that induce a change in calcium levels.

关键词： Calcium signaling/transport defense responses plant-microbe interactions signal transduction Arabidopsis.

TestDaF: Neuer Mündlicher Ausdruck ab November 2005

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德语学习 2005年第3期 50-55页

作者： stefanie Eschenlohr Vertreterin des TestdaF-Instituts in der VR China Dr.

Uberblick Bei der nǎichsten TestDaF-Prüfung in China am 6. November 2005 wird es erstmals ein neues Format des Prüfungsteils Mündlicher Ausdruck geben. Der Prüfungsteil wird nicht mehraus zehn, sondern nur noch aus sieben Aufgaben bestehen. Zum Tell sind die Aufgaben inhaltlich umfangreicher, dafür steht Ihnen aber auch mehr Vorbereitungs- und mehr Sprechzeit zur Verfügung.

关键词： DaF 德福考试出国留学德语应试指导

“Macrobot”:An Automated Segmentation-Based System for Powdery Mildew Disease Quantification

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Plant Phenomics 2020年第1期2卷 334-346页

作者： stefanie Lück Marc Strickert Maximilian Lorbeer Friedrich Melchert Andreas Backhaus David Kilias Udo Seiffert Dimitar Douchkov Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) Correnstr.306466 SeelandGermany Physics Institute II University of GiessenHeinrich-Buff-Ring 1635392 GiessenGermany Julius Kühn Institute for National and International Plant Health Messeweg 11/1238104 BraunschweigGermany Fraunhofer Institute for Factory Operation and Automation(IFF) Sandtorstr.2239106 MagdeburgGermany

Managing plant diseases is increasingly difficult due to reasons such as intensifying the field production,climatic change-driven expansion of pests,redraw and loss of effectiveness of pesticides,rapid breakdown of the disease resistance in the field,and other *** substantial progress in genomics of both plants and pathogens,achieved in the last decades,has the potential to counteract this negative trend,however,only when the genomic data is supported by relevant phenotypic data that allows linking the genomic information to specific *** have developed a set of methods and equipment and combined them into a“Macrophenomics facility.”The pipeline has been optimized for the quantification of powdery mildew infection symptoms on wheat and barley,but it can be adapted to other diseases and host *** Macrophenomics pipeline scores the visible powdery mildew disease symptoms,typically 5-7 days after inoculation(dai),in a highly automated *** system can precisely and reproducibly quantify the percentage of the infected leaf area with a theoretical throughput of up to 10000 individual samples per day,making it appropriate for phenotyping of large germplasm collections and crossing populations.

关键词： linking pesticides typically

Sprachprfungen zum Hochschulzugang: Neues zu TestDaF und DSH

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德语人文研究 2004年第5期 43-48页

作者： stefanie Eschenlohr TestDaF Institut Representative in P.R. China Dr.

Diploid species phylogeny and evolutionary reticulation indicate early radiation of Ephedra in the Tethys coast

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Journal of Integrative Plant Biology 2023年第12期65卷 2619-2630页

作者： Qiong Yu Fu-Sheng Yang Ya-Xing Chen Hui Wu stefanie M.Ickert-Bond Xiao-Quan Wang State Key Laboratory of Plant Diversity and Specialty Crops Institute of Botanythe Chinese Academy of SciencesBeijing100093 China China National Botanical Garden Beijing100093 China College of Life Sciences University of Chinese Academy of SciencesBeijing100049 China Department of Biology and Wildlife&UA Museum of the North University of Alaska Fairbanks(UAF)FairbanksAK99775 USA

Reconstructing a robust species phylogeny and disentangling the evolutionary and biogeographic history of the gymnosperm genus Ephedra,which has a large genome and rich polyploids,remain a big *** we reconstructed a transcriptome-based phylogeny of 19 diploid Ephedra species,and explored evolutionary reticulations in this genus represented by 50 diploid and polyploid species,using four low-copy nuclear and nine plastid *** diploid species phylogeny indicates that the Mediterranean species diverged first,and the remaining species split into three clades,including the American species(Clade A),***,and all other Asian species(Clade B).The single-gene trees placed *** sister to Clade A,Clade B,or Clades A+B in similar proportions,suggesting that radiation and gene flow likely occurred in the early evolution of *** addition,reticulate evolution occurred not only among the deep nodes,but also in the recently evolved South American species,which further caused difficulty in phylogenetic ***,we found that allopolyploid speciation was pervasive in *** study also suggests that Ephedra very likely originated in the Tethys coast during the late Cretaceous,and the South American Ephedra species have a single origin by dispersal from Mexico or North America.

关键词： allopolyploidy biogeography Ephedra phylogenomics phylogeny reticulate evolution

Testing experimentally the effect of soil resource mobility on plant competition

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Journal of Plant Ecology 2014年第3期7卷 276-286页

作者： stefanie Wilberts Matthias Suter Nina Walser Peter J.Edwards Harry Olde Venterink Dieter Ramseier Department of Biology/Chemistry Division of EcologyUniversity of OsnabrückBarbarastrasse 13Osnabrück 49076Germany Forage Production and Grassland Agroscope Reckenholz-TänikonReckenholzstrasse 1918046 ZurichSwitzerland Institute of Integrative Biology ETH ZurichUniversitätstrasse 16Zürich 8092Switzerland Plant Biology and Nature Management Vrije Universiteit BrusselPleinlaan 21050 BrusselsBelgium

Aims The volume of soil beyond a plant’s roots from which that plant is able to acquire a particular nutrient depends upon the mobility of that nutrient in the *** this reason it has been hypothesized that the strength of competitive interactions between plants vary with soil nutrient *** aimed to provide an experimental test of this *** We devised two experimental systems to investigate specifically the effect of nutrient transport rates upon intraspecific *** the first,the exchange of rhizosphere water and dissolved nutrients between two connected pots,each containing one plant,was manipulated by alternately raising and lowering the *** the second experiment,the roots systems of two competing plants were separated by partitions of differing porosity,thereby varying the plants’access to water and nutrients in the other plant's *** this second experiment,we also applied varying amounts of nutrients to test whether higher nutrient input would reduce competition when competition for light is avoided,and applied different water levels to affect nutrient concentrations without changing nutrient *** findings In both experiments,lower mobility reduced competitive effects on plant biomass and on relative growth rate(RGR),as *** the second experiment,however,competition was more intense under high nutrient input,suggesting that low nutrient supply rates reduced the strength of the superior *** effects on RGR were only evident under the low water level,suggesting that under lower nutrient concentrations,competitive effects might be less *** together,our results provide the first direct experimental evidence that a reduction in nutrient mobility can reduce the intensity of competition between plants.

关键词： belowground competition nutrient transport soil water relative growth rate

Acupuncture treatment in gastrointestinal diseases: A systematic review

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World Journal of Gastroenterology 2007年第25期13卷 3417-3424页

作者： Antonius Schneider Konrad Streitberger stefanie Joos University Hospital Heidelberg Department of General Practice and Health Services Research Germany University Hospital Heidelberg Department of Anaesthesiology Germany

The purpose of this work was to assess the evidence for effectiveness of acupuncture （AC） treatment in gastrointestinal diseases. A systematic review of the Medline-cited literature for clinical trials was performed up to May 2006. Controlled trials assessing acupuncture point stimulation for patients with gastrointestinal diseases were considered for inclusion. The search identified 18 relevant trials meeting the inclusion criteria. Two irritable bowel syndrome （IBS） trials, 1 Crohn＇s disease and 1 colitis ulcerosa trial had a robust random controlled trial （RCT） design. In regard to other gastrointestinal disorders, study quality was poor. In all trials, quality of life （QoL） improved significantly independently from the kind of acupuncture, real or sham. Real AC was significantly superior to sham acupuncture with regard to disease activity scores in the Crohn and Colitis trials. Efficacy of acupuncture related to QoL in IBS may be explained by unspecific effects. This is the same for QoL in inflammatory bowel diseases （IBD）, whereas specific acupuncture effects may be found in clinical scores. Further trials for IBDs and in particular for all other gastrointestinal disorders would be necessary to evaluate the efficacy of acupuncture treatment. However, it must be discussed on what terms patients benefit when this harmless and obviously powerful therapy with regard to QoL is demystified by further placebo controlled trials.

关键词： Irritable bowel syndrome Inflammatorybowel disease Gastrointestinal disease Placebo Acupuncture

A comparison of perspectives on costs in emergency care among emergency department patients and residents

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World Journal of Emergency Medicine 2017年第1期8卷 39-42页

作者： stefanie K.Gilbert Leana S.Wen Jesse M.Pines Department of Emergency Medicine The George Washington UniversityWashingtonDC 20037USA

BACKGROUND: Costs of care are increasingly important in healthcare policy and, more recently, in clinical care in the emergency department(ED). We compare ED resident and patient perspectives surrounding costs in emergency ***: We conducted a mixed methods study using surveys and qualitative interviews at a single, academic ED in the United States. The two study populations were a convenience sample of adult ED patients(>17 years of age) and ED residents training at the same institution. Participants answered open- and closed-ended questions on costs, medical decision making, cost-related compliance, and communication about costs. Closed-ended data were tabulated and described using standard statistics while open-ended responses were analyzed using grounded ***: Thirty ED patients and 24 ED residents participated in the study. Both patients and residents generally did not have knowledge of medical costs. Patients were comfortable discussing costs while residents were less comfortable. Residents agreed that doctors should consider costs when making medical decisions whereas patients somewhat disagreed. Additionally, residents generally took costs into consideration during clinical decision-making, yet nearly all residents agreed that they had too little education on ***: There were several notable differences in ED patient and resident perspectives on costs in this U.S. sample. While patients somewhat disagree that cost should factor into decision making, generally they are comfortable discussing costs yet report having insuf? cient knowledge of what care costs. Conversely, ED residents view costs as important and agree that cost should factor into decision making but lack education on what emergency care costs.

关键词： Emergency medicine Clinical decision-making Cost Communication Residency education

Ionomic Profiling of Rice Genotypes and Identification of Varieties with Elemental Covariation Effects

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Rice science 2022年第1期29卷 76-88,I0026-I0030页

作者： ZHANG Chengming Nobuhiro TANAKA Maria stefanie Dw IYANTI Matthew SHENTON Hayato MARUYAMA Takuro SHINANO CHU Qingnan XIE Jun Toshihiro WATANABE Research Faculty of Agriculture Hokkaido UniversitySapporo 060-8589Japan Institute of Crop Science National Agriculture and Food Research OrganizationTsukuba 305-8518Japan Graduate School of Agricultural and Life Sciences The University of TokyoTokyo 113-8657Japan College of Resources and Environment Southwest UniversityChongqing 400715China

Ionomic profiles are primarily influenced by genetic and environmental *** ionomic responses to varietal effects is necessary to understand the ionomic variations among species or subspecies and to potentially understand genetic effects on ionomic *** cultivated 120 rice(Oryza sativa)varieties to seedling stage in identical hydroponic conditions and determined the concentrations of 26 elements(including 3 anions)in the shoots and roots of *** the subspecies effects were limited by the genus Oryza pre-framework and its elemental chemical properties,we found significant differences in ionomic variations in most elements among the aus,indica and japonica *** component analysis of the correlations indicated that variations in the root-to-shoot ionomic transport mechanisms were the main causes of ionomic differences among the ***,the correlations were primarily associated with the screening of varieties for elemental covariation effects that can facilitate breeding biofortified rice varieties with safe concentrations of otherwise toxic *** japonica subspecies exhibited the strongest elemental correlations and elemental covariation effects,therefore,they showed greater advantages for biofortification than the indica and aus subspecies,whereas indica and aus subspecies were likely safer in metal(loid)polluted *** also found that geographical and historical distribution significantly defined the ionomic ***,the results of this study provided a reference for further association studies to improve the nutritional status and minimize toxicity risks in rice production.

关键词： ionomic profile rice genotype elemental covariation correlation principal component analysis