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Rbfox1 controls alternative splicing of focal adhesion genes in cardiac muscle cells

Journal of Molecular Cell Biology 2024年第1期16卷 54-65页

作者： Peter Zorn Jaime Calvo Sánchez Tala Alakhras Barbara Schreier Michael Gekle stefan h.ttelmaier Marcel Köhn Junior Group‘Non-coding RNAs and RBPs in human Diseases’ Medical FacultyUniversity of Halle–Wittenberg06120 Halle(Saale)Germany Julius Bernstein Institute of Physiology Medical FacultyUniversity of Halle–Wittenberg06112 Halle(Saale)Germany Institute of Molecular Medicine Medical FacultyUniversity of Halle–Wittenberg06120 Halle(Saale)Germany

Alternative splicing is one of the major cellular processes that determine the tissue-specific expression of protein ***,it remains challenging to identify physiologically relevant and tissue-selective proteins that are generated by alternative ***,we investigated the target spectrum of the splicing factor Rbfox1 in the cardiac muscle context in more *** using a combination of in silico target prediction and in-cell validation,we identified several focal adhesion proteins as alternative splicing targets of *** focused on the alternative splicing patterns of vinculin(metavinculin isoform)and paxillin(extended paxillin isoform)and identified both as potential Rbfox1 *** analyses suggested that both isoforms are promoted by Rbfox1 due to binding in the *** adhesions play an important role in the cardiac muscle context,since they mainly influence cell shape,cytoskeletal organization,and cell–matrix *** data confirmed that depletion of Rbfox1 changed cardiomyoblast morphology,cytoskeletal organization,and multinuclearity after differentiation,which might be due to changes in alternative splicing of focal adhesion ***,our results indicate that Rbfox1 promotes alternative splicing of focal adhesion genes in cardiac muscle cells,which might contribute to heart disease progression,where downregulation of Rbfox1 is frequently observed.

关键词： Rbfox1 alternative splicing focal adhesion cardiac muscle cells

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Deformable microlaser force sensing

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Light(Science & Applications) 2024年第6期13卷 1196-1209页

作者： Eleni Dalaka Joseph Shill Jonathan h.h.Booth Anna Popczyk stefan R.Pulver Malte C.Gather Marcel Schubert Centre of Biophotonics SUPASchool of Physics and AstronomyUniversity of St AndrewsNorth HaughSt AndrewsUK humboldt Centre for Nano-and Biophotonics Department of ChemistryUniversity of CologneKölnGermany School of Psychology and Neuroscience University of St AndrewsSt Mary’s QuadSouth StreetSt AndrewsUK Institute for Bioengineering of Catalonia BarcelonaSpain

Mechanical forces are key regulators of cellular behavior and function,affecting many fundamental biological processes such as cell migration,embryogenesis,immunological responses,and pathological *** force sensors and imaging techniques have been developed to quantify these otherwise invisible forces in single cells and in ***,current techniques rely heavily on high-resolution microscopy and do not allow interrogation of optically dense tissue,reducing their application to 2D cell cultures and highly transparent biological ***,we introduce DEFORM,deformable microlaser force sensing,a spectroscopic technique that detects sub-nanonewton forces with unprecedented spatio-temporal *** is based on the spectral analysis of laser emission from dye-doped oil microdroplets and uses the force-induced lifting of laser mode degeneracy in these droplets to detect nanometer *** validation by atomic force microscopy and development of a model that links changes in laser spectrum to applied force,DEFORM is used to measure forces in 3D and at depths of hundreds of microns within tumor spheroids and late-stage Drosophila *** furthermore show continuous force sensing with single-cell spatial and millisecond temporal resolution,thus paving the way for non-invasive studies of biomechanical forces in advanced stages of embryogenesis,tissue remodeling,and tumor invasion.

关键词： invasion otherwise hundreds

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CO_(2)capture costs of chemical looping combustion of biomass:A comparison of natural and synthetic oxygen carrier

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Journal of Energy Chemistry 2024年第5期92卷 296-310页

作者： Benjamin Fleiß Juraj Priscak Martin hammerschmid Josef Fuchs stefan Müller hermann hofbauer Institute of Chemical Environmental and Bioscience EngineeringTU WienVienna1060 ViennaAustria BEST–Bioenergy and Sustainable Technologies Gmbh Graz8010 StyriaAustria

Chemical looping combustion has the potential to be an efficient and low-cost technology capable of contributing to the reduction of the atmospheric concentration of CO_(2) in order to reach the 1.5/2°C goal and mitigate climate *** this process,a metal oxide is used as oxygen carrier in a dual fluidized bed to generate clean CO_(2) via combustion of *** commonly,natural ores or synthetic materials are used as oxygen carrier whereas both must meet special requirements for the conversion of solid *** oxygen carriers are characterized by higher reactivity at the expense of higher costs versus the lower-cost natural *** determine the viability of both possibilities,a techno-economic comparison of a synthetic material based on manganese,iron,and copper to the natural ore ilmenite was *** synthetic oxygen carrier was characterized and tested in a pilot plant,where high combustion efficiencies up to 98.4%and carbon capture rates up to 98.5%were *** techno-economic assessment resulted in CO_(2) capture costs of 75 and 40€/tCO_(2) for the synthetic and natural ore route respectively,whereas a sensitivity analysis showed the high impact of production costs and attrition rates of the synthetic *** synthetic oxygen carrier could break even with the natural ore in case of lower production costs and attrition rates,which could be reached by adapting the production process and recycling *** comparison to state-of-the-art technologies,it is demonstrated that both routes are viable and the capture cost of CO_(2) could be reduced by implementing the chemical looping combustion technology.

关键词： Chemical looping combustion BECCS Techno-economic assessment CO_(2)capture costs Oxygen carrier development Synthetic materials Ilmenite

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POLIII-derived non-coding RNAs acting as scaffolds and decoys

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Journal of Molecular Cell Biology 2019年第10期11卷 880-885页

作者： hendrikTauber stefan h.ttelmaier Marcel Kohn Institute of Molecular Medicine Section for Molecular Cell BiologyFaculty of MedicineMartin Luther University Halle-WittenbergCharles Tan ford Protein CentreKurt-Mothes-Str.3a06120 HalleGermany Julius Bernstein Institute of Physiology Faculty of MedicineMartin Luther University Halle-WittenbergCharles Tan ford Protein CentreKurt-Mothes-Str.3a06120 HalleGermany

A large variety of eukaryotic small structured POLIII-derived non-coding RNAs(ncRNAs)have been described in the ***,for only few,e.g.7SL and h1/MRP families,cellular functions are well *** the vast majority of these transcripts,cellular functions remain *** findings on the role ofY RNAs and other POLIII-derived ncRNAs suggest an evolutionarily conserved function of these ncRNAs in the assembly and function of ribonucleoprotein complexes(RNPs).These RNPs provide cellular‘machineries’,which are essential for guiding the fate and function of a variety of *** this review,we summarize current knowledge on the role of POLIII-derived ncRNAs in the assembly and function of *** propose that these ncRNAs serve as scaffolding factors that‘chaperone’RNA-binding proteins(RBPs)to form functional *** addition or associated with this role,some small ncRNAs act as molecular decoys impairing the RBP-guided control of RNA fate by competing with other RNA *** suggests that POLIII-derived ncRNAs serve essential and conserved roles in the assembly of larger RNPs and thus the control of gene expression by indirectly guiding the fate of mRNAs and IncRNAs.

关键词： ncRNA POLIII RBP scaffold RBP decoy

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Optical probing of ultrafast laser-induced solid-to-overdense-plasma transitions

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Light(Science & Applications) 2024年第5期13卷 973-984页

作者： Yasmina Azamoum Georg Alexander Becker Sebastian Keppler Guillaume Duchateau stefan Skupin Mickael Grech Fabrice Catoire Sebastian hell Issa Tamer Marco hornung Marco hellwing Alexander Kessler Franck Schorcht Malte Christoph Kaluza GSI helmholtzzentrum für Schwerionenforschung Planckstr.164291 DarmstadtGermany. helmholtz Institute Jena Fröbelstieg 307743 JenaGermany. Institute of Optics and Quantum Electronics Friedrich-SchillerUniversität JenaMax-Wien-Platz 107743 JenaGermany. CEA-CESTA 15 Avenue des SablièresCS6000133116 Le BarpCedexFrance Institut Lumière Matière UMR 5306-CNRSUniversitéde Lyon 169622 VilleurbanneFrance. LULI CNRSCEASorbonne UniversitéInstitut Polytechnique de ParisPalaiseauFrance. Universitéde Bordeaux-CNRS-CEA CELIAUMR 5107 TalenceFrance

Understanding the solid target dynamics resulting from the interaction with an ultrashort laser pulse is a challenging fundamental multi-physics problem involving atomic and solid-state physics,plasma physics,and laser *** of the initial interplay of the underlying processes is essential to many applications ranging from lowpower laser regimes like laser-induced ablation to high-power laser regimes like laser-driven ion *** the properties of the so-called pre-plasma formed as the laser pulse’s rising edge ionizes the target is complicated from the theoretical and experimental point of view,and many aspects of this laser-induced transition from solid to overdense plasma over picosecond timescales are still open *** the one hand,laser-driven ion acceleration requires precise control of the pre-plasma because the efficiency of the acceleration process crucially depends on the target properties at the arrival of the relativistic intensity peak of the *** the other hand,efficient laser ablation requires,for example,preventing the so-called“plasma shielding”.By capturing the dynamics of the initial stage of the interaction,we report on a detailed visualization of the pre-plasma formation and ***-thin diamond-like carbon foils are shown to transition from solid to plasma during the laser rising edge with intensitieshot near-infrared probe transmission measurements evidence sub-picosecond dynamics of an expanding plasma with densities above 10^(23)cm^(−3)(about 100 times the critical plasma density).The complementarity of a solid-state interaction model and kinetic plasma description provides deep insight into the interplay of initial ionization,collisions,and expansion.

关键词： laser acceleration solid

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Discrete Boltzmann model with split collision for nonequilibrium reactive flows

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Communications in Theoretical Physics 2024年第8期76卷 164-186页

作者： Chuandong Lin Kai h Luo huilin Lai Sino-French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China Key Laboratory for Thermal Science and Power Engineering of Ministry of Education Department of Energy and Power EngineeringTsinghua UniversityBeijing 100084China Department of Mechanical Engineering National University of Singapore10 Kent Ridge Crescent119260Singapore Department of Mechanical Engineering University College LondonTorrington PlaceLondon WC1E 7JEUnited Kingdom School of Mathematics and Statistics Key Laboratory of Analytical Mathematics and Applications(Ministry of Education)Fujian Key Laboratory of Analytical Mathematics and Applications(FJKLAMA)Center for Applied Mathematics of Fujian Province(FJNU)Fujian Normal University350117 FuzhouChina

A multi-relaxation-time discrete Boltzmann model(DBM) with split collision is proposed for both subsonic and supersonic compressible reacting flows, where chemical reactions take place among various components. The physical model is based on a unified set of discrete Boltzmann equations that describes the evolution of each chemical species with adjustable acceleration, specific heat ratio, and Prandtl number. On the right-hand side of discrete Boltzmann equations, the collision,force, and reaction terms denote the change rates of distribution functions due to self-and crosscollisions, external forces, and chemical reactions, respectively. The source terms can be calculated in three ways, among which the matrix inversion method possesses the highest physical accuracy and computational efficiency. Through Chapman-Enskog analysis, it is proved that the DBM is consistent with the reactive Navier-Stokes equations, Fick's law and the stefan-Maxwell diffusion equation in the hydrodynamic limit. Compared with the one-step-relaxation model, the split collision model offers a detailed and precise description of hydrodynamic, thermodynamic, and chemical nonequilibrium effects. Finally, the model is validated by six benchmarks, including multicomponent diffusion, mixture in the force field, Kelvin-helmholtz instability, flame at constant pressure, opposing chemical reaction, and steady detonation.

关键词： discrete Boltzmann method reactive flow detonation nonequilibrium effect

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Exploring fs-laser irradiation damage subthreshold behavior of dielectric mirrors via electrical measurements

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high Power Laser Science and Engineering 2024年第2期12卷 24-32页

作者： Petrisor Gabriel Bleotu Radu Udrea Alice Dumitru Olivier Uteza Maria-Diana Mihai Dan Gh Matei Daniel Ursescu stefan Irimiciuc Valentin Craciun Extreme Light Infrastructure-Nuclear Physics(ELI-NP) Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering(IFIN-HH)MagureleRomania Doctoral School of Physics University of BucharestMagureleRomania LULI-CNRS CEAUniversite SorbonneEcole PolytechniqueInstitut Polytechnique de ParisPalaiseau CEDEXFrance Apel Laser IlfovRomania Aix-Marseille University CNRSLP3 UMR 7341MarseilleFrance horia hulubei National Institute for R&D in Physics and Nuclear Engineering(IFIN-hh) MagureleRomania University Politehnica of Bucharest BucharestRomania National Institute for Lasers Plasma and Radiation Physics MagureleRomania

With ultrafast laser systems reaching presently 10 PW peak power or operating at high repetition rates,research towards ensuring the long-term,trouble-free performance of all laser-exposed optical components is *** work is focused on providing insight into the optical material behavior at fluences below the standardized laser-induced damage threshold(LIDT)value by implementing a simultaneous dual analysis of surface emitted particles using a Langmuir probe(LP)and the target current(TC).hfO_(2) and ZrO_(2) thin films deposited on fused silica substrates by pulsed laser deposition at various O_(2) pressures for defect and stoichiometry control were irradiated by Gaussian,ultrashort laser pulses(800 nm,10 hz,70 fs)in a wide range of *** TC and LP collected signals were in good agreement with the existing theoretical description of laser–matter interaction at an ultrashort time *** approach for an in situ LIDT monitoring system provides measurable signals for below-threshold irradiation conditions that indicate the endurance limit of the optical surfaces in the single-shot energy scanning *** LIDT value extracted from the LP-TC system is in line with the multipulse statistical analysis done with ISO 21254-2:2011(E).The implementation of the LP and TC as on-shot diagnostic tools for optical components will have a significant impact on the reliability of next-generation ultrafast and high-power laser systems.

关键词： hfO_(2) in situ detection Langmuir probe laser-induced damage threshold target current ZrO_(2)

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Is Being Funny a Useful Policy?how Local Governments' humorous Crisis Response Strategies and Crisis Responsibilities Influence Trust,Emotions,and Behavioral Intentions

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International Journal of Disaster Risk Science 2022年第5期13卷 676-690页

作者： Janna hämpke stefan Röseler Meinald T.Thielsch Department of Psychology University of Münster48149 MünsterGermany

This study is the first to investigate how a local government’s humorously framed response strategy on social media to a low-severity crisis influences people ’s trust in the local government and their crisis-related behavioral intentions,specific ally when considering the government’s responsibility for the *** on the situational crisis communication theory,we examined the mediating role of experienced positive or negative affect on people’ s responses to a local government’s crisis communication ***,we exploratorily examined the predictive power and moderating role of demographics,sense of humor,disposition to trust,and the respective crisis scenarios.A total of517 people participated in an online experiment in which they were confronted with three randomly presented fictive crisis scenarios where the local government’s crisis responsibility(high versus low) and the framing of their crisis response strategy(in form of humorous versus rational Twitter posts) were systematically varied between ***,the results mostly corroborate earlier findings about the degree of crisis responsibility(that is,when a government’s crisis responsibility is high,people have less trust and behavioral intentions) and about the mediating role of experienced ***,we found that humorously framed strategies negatively influence trust and positive affect(but not behavioral intentions).In contrast to earlier findings,the crisis responsibility × framing interaction was not ***,the results advise against using humor in crisis communications on social media,even in lowseverity *** analyses indicate that further investigations should focus on specific crisis characteristics and potential moderators.

关键词： Afect Crisis communication Crisis responsibility humor Situational crisis communication theory Social media

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Treatment of early gastric cancer in the Western World

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World Journal of Gastroenterology 2014年第19期20卷 5672-5678页

作者： Elfriede Bollschweiler Felix Berlth Christoph Baltin stefan Mnig Arnulf h hlscher Department of General Visceral and Cancer SurgeryUniversity of Cologne50937 CologneGermany

The incidence rate of gastric cancer is much higher in Asia than in the Western industrial *** to the different screening programs in Japan and Korea about fifty percent of treated patients had an early tumor *** contrast,European and American patients with gastric cancer had an advanced tumor ***,the experience for the various therapeutic options for gastric cancer may be different between these *** this review we tried to point out the treatment modalities in Western industrial countries for early gastric cancer.

关键词： Gastric cancer Early cancer Epidemiology Diagnosis Therapy Western World

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Simulation of spray coating in a spouted bed using recurrence CFD

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Particuology 2019年第1期17卷 92-103页

作者： Paul Kieckhefena Thomas Lichtenegger Swantje Pietsch stefan Pirker stefan heinrich Institute of Solids Process Engineering and Particle Technology Hamburg University of Technology. HamburgGermany Department for Particulate Flow Modelling. Johannes Kepler University LinzAustria Linz Institute of Technology (LIT) Johannes Kepler UniversityLinzAustria

Although numerical models such as the computational fluid dynamics–discrete element method (CFD–DEM) have enabled the accurate simulation of laboratory-scale apparatuses, the application of these methods to large-scale apparatuses with many particles and time scales ranging from minutes to hours remains a challenge. The recently developed recurrence CFD (rCFD) method seeks to overcome these issues in pseudo-periodic processes by extrapolating globally recurring patterns in a physically meaningful way and describing the transport and interaction of passive scalars using Lagrangian tracers. Spouted beds represent an interesting target because of the associated variety of flow regimes. They can be effectively described by CFD–DEM on the time scale of tens of seconds, whereas industrially relevant processes typically take hours. In this contribution, we established the validity of applying the Lagrangian rCFD method to spouted beds by demonstrating the accurate reproduction of the particle residence time distribution in a fictitious spray zone. The deposition of spray droplets onto tracer particles was simulated for 1 h, and the particle surface coverage distribution was estimated using a statistical approach for both an unstabilized prismatic spouted bed and one stabilized by draft plates.

关键词： CFD–DEM Recurrence CFD Spray coating Time-scale decoupling Spouted bed Draft plate

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