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Fstl1 Is a BMP4 Signaling Antagonist in Controlling Mouse Lung Development

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Fstl1 Is a BMP4 Signaling Antagonist in Controlling Mouse Lu...

花青素能减轻阿尔茨海默症模型细胞的氧化损伤(英文)

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Yan Geng~(*+),Yingying Dong~(*+),Ye-Guang Chen~(*),and Wen Ning~(*‖**) +Model Animal Research Center,Nanjing university,Nanjing 210061,china state key laboratory of biomembrane and membrane biotechnology,school of life sciences,tsinghua university,beijing 100084,china ‖College of life sciences,Nankai university,Tianjin 300071,china.

Lung morphogenesis is a well-orchestrated,tightly regulated process through several molecular pathways including TGF/BMP *** of these signaling pathways leads to lung malformation. We herein investigated the role of Follistatin-like 1(Fstl1),a secreted follistatin-module containing glycoprotein,in lung *** of Fstl1 in mice led to postnatal lethality due to respiratory *** of the mutant phenotype showed that Fstl1 is essential for tracheal cartilage formation and alveolar *** of the Fstl1 gene resulted in malformed tracheal rings manifested as discontinued rings and reduced ring ***1 deficient mice displayed septal hypercellularity and end-expiratory atelectasis,which were associated with impaired differentiation of distal alveolar epithelial cells and insufficient production of mature surfactant ***,Fstl1 interacted directly with BMP4,negatively regulated BMP4/Smad 1/5/8 signaling,and inhibited BMP4-induced surfactant gene *** BMP signaling activity by Noggin rescued pulmonary atelectasis of Fstl1 deficient ***,we provide in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation,in part,through BMP4 signaling.

关键词： Fstll respiratory failure tracheal cartilage differentiation of alveolar epithelial cell BMP4

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生物化学与生物物理进展 2010年第7期37卷 779-785页

作者：刘玲玲盛柏杨闫玉芳龚锴马拓赵南明张秀芳公衍道清华大学生命科学学院生物膜与膜生物工程国家重点实验室北京100084

氧化胁迫在阿尔茨海默症(AD)的发病过程中起重要作用.花青素是一种广泛存在于植物中的黄酮类物质.实验结果表明,100μmol/L的花青素可以有效地缓解AD模型细胞氧化胁迫,具体表现为,降低AD模型细胞的H2O2易感性,减少胞内ROS和[NO]i.c-Jun... 详细信息

氧化胁迫在阿尔茨海默症(AD)的发病过程中起重要作用.花青素是一种广泛存在于植物中的黄酮类物质.实验结果表明,100μmol/L的花青素可以有效地缓解AD模型细胞氧化胁迫,具体表现为,降低AD模型细胞的H2O2易感性,减少胞内ROS和[NO]i.c-Jun氨基端激酶(JNK)是氧化胁迫导致细胞损伤的重要信号途径.实验结果显示花青素可以有效地抑制氧化胁迫对JNK的激活.提示花青素的抗氧化胁迫作用与JNK信号途径有关.因此,花青素可以作为一种氧化胁迫因子的清除剂来保护AD模型细胞,有望用于AD的辅助治疗.

关键词：阿尔茨海默症氧化胁迫花青素 c-Jun氨基端激酶(JNK)

淀粉样肽Aβ导致线粒体功能紊乱的体内和体外研究

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生物化学与生物物理进展 2010年第2期37卷 154-160页

作者：刘玲玲盛柏杨龚锴赵南明张秀芳唐佩福公衍道清华大学生命科学学院生物膜与膜生物工程国家重点实验室北京100084 解放军总医院骨科北京100853

为探索阿尔茨海默症(AD)中β淀粉样肽(Aβ)对线粒体功能的影响,比较了稳定表达人野生型淀粉样前体蛋白(APP)的细胞和同时转入人Swedish突变APP及ΔE9突变PS1的双转细胞(swe.Δ9)的线粒体功能.结果发现,swe.Δ9细胞的线粒体膜电位、细胞... 详细信息

为探索阿尔茨海默症(AD)中β淀粉样肽(Aβ)对线粒体功能的影响,比较了稳定表达人野生型淀粉样前体蛋白(APP)的细胞和同时转入人Swedish突变APP及ΔE9突变PS1的双转细胞(swe.Δ9)的线粒体功能.结果发现,swe.Δ9细胞的线粒体膜电位、细胞色素c氧化酶活性、线粒体膜流动性、ATP含量均明显低于APP细胞,而APP细胞又明显低于对照的转入空质粒的细胞.在转基因小鼠上也得到类似结果:同时转入人Swedish突变APP和人PS1 M146V敲入的双转小鼠的细胞色素c氧化酶活性和ATP含量比只转入Swedish突变APP的Tg2576小鼠更低.结果证明了AD模型中线粒体功能损害程度与Aβ产量的正相关关系.

关键词：阿尔茨海默症线粒体 β淀粉样蛋白线粒体膜电位细胞色素c氧化酶活性线粒体膜流动性 ATP

Negative regulation of TGF-β signaling in development

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Cell Research 2004年第6期14卷 441-449页

作者： Ye Guang CHEN, An Ming MENG The state key laboratory of biomembrane and membrane biotechnology, Department of Biological sciences and biotechnology, tsinghua university, beijing 100084, china Department of Biological sciences and biotechnology The State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing 100084 China

The TGF-β superfamily members have important roles in controlling patterning and tissue formation in both invertebrates and vertebrates. Two types of signal transducers, receptors and Smads, mediate the signaling to regulate expression of their target genes. Despite of the relatively simple signal transduction pathway, many modulators have been found to contribute to a tight regulation of this pathway in a variety of mechanisms. This article reviews the negative regulation of TGF-β signaling with focus on its roles in vertebrate development.

关键词： TGF-β BMP Nodal Activin antagonist development negative regulation.

Electrophysiological research of synapse formation from beginning to maturity

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Chinese Science Bulletin 1998年第1期43卷 55-59页

作者： XIE Zuoping and ZHENG Yufang state key laboratory of biomembrane and membrane Biotechnique Department of Biological sciences and biotechnology, tsinghua university, beijing 100084, china state key laboratory of biomembrane and membrane Biotechnique Tsinghua University Beijing China Department of Biological sciences and biotechnology Tsinghua University Beijing China

The development of synapses is an important question in neurobiology. We employ patchclamp technique to record MEPCs and EPCs of myoballs in Xenopus cell culture. The rate of EPC/MEPC is related with synapses maturation. Accordingly, we define the mature synapses as those whose rate of presynaptic membrane’s EPC to MEPC is more than 8. The process of maturation needs about 2-5 h after neurites contact with myoballs. We divide the development of synapses into three stages: young, developing and mature. The neurotransmitters stack gradually and increase by hundreds of times along with young synapses growing into maturity. So the process of synapses maturation should include presynaptic membrane specialization, postsynaptic membrane specialization and neurotransmitters stack.

关键词： definition of mature synapse α latrotoxin (α LTX) neurotransmitter stack.

A measurement error model for microarray data analysis

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Progress in Natural Science:Materials International 2005年第7期15卷 614-620页

作者： ZHOU Yiming and CHENG Jing ( Department of Biological sciences and biotechnology, tsinghua university, beijing 100084, china state key laboratory of biomembrane and membrane biotechnology, tsinghua university, beijing 100084, china National Engineering Research Center for beijing Biochip Technology, 18 life Science Parkway, beijing 102206, china) Department of Biological sciences and biotechnology Tsinghua University Beijing 100084 China. state key laboratory of biomembrane and membrane biotechnology Tsinghua University Beijing 100084 China. National Engineering Research Center for beijing Biochip Technology 18 Life Science Parkway Beijing 102206 China

Microarray technology has been widely used to analyze the gene expression levels by detecting fluorescence intensity in a high throughput fashion. However, since the measurement error produced from various sources in microarray experiments is heterogeneous and too large to be ignored, we propose here a measurement error model for microarray data processing, by which the standard deviation of the measurement error is demonstrated to be linearly increased with fluorescence intensity. A robust algorithm, which estimates the parameters of the measurement error model from a single microarray without replicated spots, is provided. The model and algorithm for estimating of the parameters from a given data set are tested on both the real data set and the simulated data set, and the result has been proven satisfactory. And, combining the measurement error model with traditional Z-test method, a full statistical model has been developed It can significantly improve the statistical inference for identifying differentially expressed genes.

关键词： microarray measurement error model parameter estimation differentially expressed genes.

Structure and regulatory function of plant transcription factors

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Chinese Science Bulletin 2001年第4期46卷 271-278页

作者： LIU Qiang, ZHANG Guiyou & CHEN ShouyiDepartment of Biological Science and biotechnology, tsinghua Univorsity, beijing 100084, china Department of Biological Science and biotechnology, state laboratory of biomembrane and membrane biotechnology, tsinghua university, beijing 100084, china Institute of Genetics, Chinese Academy of sciences, beijing 100101, china Department of Biological Science and biotechnology Tsinghua University Beijing China Department of Biological Science and biotechnology State Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

关键词： transcription factor DNA-binding domain cis-acting element.

Analysis micro-mechanism of burrs formation in the case of nanometric cutting process using numerical simulation method

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Science china(Technological sciences) 2007年第2期50卷 129-137页

作者： HAN XueSong & HU YuanZhong state key laboratory of Tribology, tsinghua university, beijing 100084, china 1. state key laboratory of Tribology Tsinghua University Beijing 100084 China

Burrs (exit failure), being one of the important factors influencing the final precision of workpiece, have been widely studied. Today, with the development of manufacturing technology, the depth of cut falls into the range of nanometer or subnanometer, there may be some different disciplines dominating the burrs generation process. Molecular dynamics (MD) emthod, which is different from continuous mechanics, plays an important role in describing microscopic world. Take the example of single crystal copper, this paper carries out MD analysis micro-mechanism of burrs generation process. The results show that the burrs geometry depends on the type of workpiece (ductile or brittle). The depth of cut is decreased in the case of positive burrs generation process while the depth of cut is increased in case of negative burrs generation process. keywords burr - molecular dynamics - micro-mechanism Supported by china Postdoctor Science Foundation (Grant No. 20060390458)

关键词： burr molecular dynamics micro-mechanism

Plant protein kinase genes induced by drought, high salt and cold stresses

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Chinese Science Bulletin 2000年第13期45卷 1153-1157页

作者： LIU Qiang , ZHANG Yong & Chen Shouyi1. Deparlmenl of Biological Science and biotechnology, tsinghua university, beijing 100084, china 2. Department of Biological Science and biotechnology, Slate key laboratory of biomembrane and membrane biotechnology, tsinghua university, beijing 100084, china 3. Institute of Genetics, Chinese Academy of sciences, beijing 100101, china Department of Biological Science and biotechnology Tsinghua University Beijing China Department of Biological Science and biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

Drought, high salt and cold are three different kinds of environment stresses that severely influence the growth, development and productivity of crops. They all decrease the water state of plant cells, and consequently result in the harm of plant from water deficit. Several genes encoding protein kinases and induced by drought, high salt and low temperature have been isolated from Arabidopsis. These protein kinases include receptor protein kinase (RPK), MAP kinases, ribosomal-protein kinases and transcription-regulation protein kinase. The expression features of these genes and the regulatory roles of these protein kinases in stress response and signal transduction are discussed.

关键词： protein kinase environment stress signal transduction gene expression.