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台湾翼状胬肉患者TNF-α和IL-1β的多态性研究

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世界核心医学期刊文摘（眼科学分册） 2005年第9期1卷 35-36页

作者： tsai y.-y. Lee H. Tseng S.-H. F.-J. tsai 张少娟 Department of Medical Genetics China Medical University Hospital No. 2 Yuh Der Road Taichung Taiwan

Purpose: Pterygium, a complex disease, is associated with ultraviolet radiation, immunoinflammatory process, genetic factors, and virus infection. Ultraviolet radiation induces secretion of proinflammatory cytokines by the ocular surface epithelium, inflammatory cells in the tear fluid, or both. Among these cytokines, tumour necrosis factor (TNF)αand interleukin (IL)-1βactivate pterygium body fibroblasts, resulting in a phenotype capable of expressing various proteinases associated with extracellular matrix remodelling, angiogenesis, and fibroblast proliferation,which are important for pterygiumformation and recurrence. The genetic factor was proposed to play a role in pterygium formation,but therewere fewstudies to clarify this proposition. For investigating genetic factors, the association between pterygium and TNF-αand IL-1βpolymorphisms is evaluated in this study. Methods: A total of 128 pterygium patients and 103 volunteers without pterygium were enrolled in this study. Polymerase chain reaction-based analysis was used to resolve the TNF-α-308 promoter, IL-1β-511 promoter, IL-1βexon 5, and IL-1 receptor antagonist (IL-1 Ra) polymorphisms. Results: There were no significant differences in the frequency of genotypes and alleles of TNF-α-308 promoter, IL-1β-511 promoter, IL-1βexon 5, and IL-1 Ra polymorphisms between both groups. Conclusions: The correlation between pterygium and TNF-α-308 promoter, IL-1β-511 promoter, IL-1βexon 5, and IL-1 Ra polymorphisms does not exist and those polymorphisms are not useful genetic markers for pterygium susceptibility. Further studies on other polymorphisms or haplotypes of TNF-αand IL-1βare necessary.

关键词： IL-1 TNF 多态性研究翼状胬肉成纤维细胞增殖细胞因子肿瘤坏死因子α 细胞外基质聚合酶链反应基因型频率

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清除高剂量离子注入中产生的表面沾污

微处理机 1980年第2期 125-129页

作者： M.y.tsai B.G.streetman 李伟

采用Auger电子分光镜（AES）检测由高剂量离子注入产生的硅上表面沾污层。纵向剖面分析证明当在注入机靶室的真空系统中用普通扩散泵油,Si+或BF2+的高剂量注入导致～100A厚的碳质表面层。注入机剩余真空的碳氢化合物的物理吸附以及辐照... 详细信息

采用Auger电子分光镜（AES）检测由高剂量离子注入产生的硅上表面沾污层。纵向剖面分析证明当在注入机靶室的真空系统中用普通扩散泵油,Si+或BF2+的高剂量注入导致～100A厚的碳质表面层。注入机剩余真空的碳氢化合物的物理吸附以及辐照感应聚合反应是形成这种沾污层的最大原因。在重注入硅中经常观察到这种沾污层可引起非均匀腐蚀特性和非欧姆接触结构。用有机溶剂清洗或过氧化物清洗溶液,在550℃下退火100分钟,并按势垒层剥除工艺估计阳极氧化及HF解吸作用。只发现HF解吸和阳极氧化对完全清除聚合层有效。实质上,用过氟化聚醚油替换靶室真空系统中的普通扩散泵油清除沾污。最近几年,在制造双极晶体管,FET源和漏区以及在其它各种应用中,对用高剂量离子注入工艺的要求日益增长。然而,已知离子束感应吸附的碳氢化合物聚合,导致在离子注入的样品（1.2）上形成碳化表面沾污层。注入机的剩余真空是碳氢分子的主要来源。沾污层的厚度随剂量的增加而增长,所以,重注入Si呈现出可注意到的沾污问题,反冲碳可产生结构上损伤浅P—n结的反相电流,并在注入退火后形成硅碳化物。为了更好的了解这种沾污问题并阐明解决方法,使用Auger电子分光镜（AES）检查在各种注入条件下制备的高剂量注入的硅表面层。估计了各种表面层的剥离技术,也分析了注入机真空系统中的注入温度效应和扩散泵油的种类。最后,估计化学腐蚀和欧姆按触结构对表面沾污的效应。

关键词：沾污厚度扩散泵油离子注入离子掺杂靶室高剂量

FANCA D1359y mutation in a patient with gastric polyposis and cancer susceptibility: A case report and review of literature

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World Journal of Gastroenterology 2018年第38期24卷 4412-4418页

作者： Jeffrey Peng Huang Johnson Lin Chi-yuan Tzen Wen-yu Huang Chia-Chi tsai Chih-Jen Chen yen-Jung Lu Kuei-Fang Chou ying-Wen Su Division of Hematology and Medical Oncology Department of Internal Medicine Mackay Memorial Hospital Department of Pathology Mackay Memorial Hospital Laboratory of Good Clinical Research Center Mackay Memorial Hospital Tamsui Branch Department of General Surgery Mackay Memorial Hospital Division of Gastroenterology Department of Internal Medicine Mackay Memorial Hospital ACT Genomics Co. Ltd.

Gastric polyposis is a rare disease. Not all polyps progress to cancer. Monoallelic mutation in Fanconi anemia(FA) genes, unlike biallelic gene mutations that causes typical FA phenotype, can increase risks of cancers in a sporadic manner. Aberrations in the FA pathway were reported in all molecular subtypes of gastric cancer. We studied a patient with synchronous gastric cancer from gastric polyposis by conducting a 13-year long-term follow up. Via pathway-driven massive parallel genomic sequencing, a germline mutation at FANCA D1359y was identified. We identified several recurrent mutations in DNA methylation(TET1, V873I), the β-catenin pathway(CTNNB1, S45F) and RHO signaling pathway(PLEKHG5, R203C) by comparing the genetic events between benign and malignant gastric polyps. Furthermore, we revealed gastric polyposis susceptible genes and genetic events promoting malignant transformation using pathway-driven targeted gene sequencing.

关键词： Gastric polyposis Gastric cancer Adenocarcinoma Fanconi’s anemia Malignant transformation

Sex Hormone-binding Globulin,Cardiometabolic Biomarkers,and Gestational Diabetes:A Longitudinal Study and Meta-analysis

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Maternal-Fetal Medicine 2020年第1期2卷 2-9页

作者： Meng-ying Li Shristi Rawal Stefanie N.Hinkle ye-yi Zhu Fasil Tekola-Ayele Michael y.tsai Si-Min Liu Cui-Lin Zhang Epidemiology Branch Division of Intramural Population Health ResearchEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentBethesda MD 20817USA Department of Nutritional Sciences School of Health ProfessionsRutgers UniversityNewark NJ 07107USA Division of Research Kaiser Permanente Northern CaliforniaOakland CA 94612USA Department of Laboratory Medicine and Pathology University of Minnesota Medical SchoolMinneapolis MN 55455USA Department of Epidemiology Brown University School of Public HealthProvidence RI 02912USA Department of Endocrinology Guangdong General Hospital and Guangdong Academy of Medical SciencesGuangdong 510080China

Objective::This study investigated the prospective associations of circulating levels of sex hormone-binding globulin(SHBG)levels with cardiometabolic biomarkers and risk of gestational diabetes(GDM)during *** also examines the longitudinal trajectory of SHBG in women with and without ***::We conducted a nested case-control study of 107 incident GDM cases and 214 matched controls within the Eunice Kennedy Shriver National Institute of Child Health and Human Development Fetal Growth Studies-Singleton *** cohort enrolled non-obese and obese women aged 18-40 years with a singleton pregnancy between 8 and 13 weeks of gestation from 2009 to *** was ascertained via medical records *** samples were drawn four times at gestational weeks 10-14,15-26,23-31,and *** prospective associations between SHBG levels and cardiometabolic biomarkers were examined using the Spearman partial correlation among the *** longitudinal trajectories of SHBG levels were examined among the cases and the ***-analysis of prospective studies were performed to examine the association between SHBG levels and GDM ***::SHBG levels at gestational weeks 10-14 were significantly inversely associated with fasting insulin(r=-0.17,P=0.01)and insulin resistance as measured by HOMA-IR(r=-0.17,P=0.01)at gestational week *** at gestational weeks 10-14 and 15-26 was lower in cases than controls(mean±standard deviation:(204.0±97.6)vs.(220.9±102.5)nmol/L,P=0.16 and(305.6±124.3)vs.(322.7±105.1)nmol/L,P=0.14,respectively),yet the differences were not *** the meta-analysis,SHBG was 41.5 nmol/L(95%confidence interval:23.9,59.1,Py lower among women with GDM than without,and each 50 nmol/L increase in SHBG was significantly associated with an odds ratio of 0.85(95%confidence interval:0.76-0.95,P=0.01)for ***::Lower SHBG levels in early pregnancy were prospectively associated with higher high insulin levels

关键词： Diabetes,gestational Sex binding hormones Cardiometabolic risk markers Cohort analysis Longitudinal measurement Meta-analysis

Localization of the VP2 Protein of Canine Parvovirus Type 2 on the Baculovirus Envelop and Its Immunogenicity in a Mouse Model

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Open Journal of Veterinary Medicine 2012年第4期2卷 178-185页

作者： Chih H. tsai Jing y. Wang Xin G. Xu De W. Tong Hsin y. Lu yi H. Chen Ming T. Chiou Ching D. Chang Hung J. Liu Agricultural Biotechnology Center National Chung Hsing University Taichung Taiwan College of Veterinary Medicine Northwest A&F University Yangling China Department of Veterinary Medicine National Pingtung University of Science and Technology Neipu Taiwan Graduate Institute of Vaccine Biotechnology Pingtung Taiwan Institute of Molecular Biology National Chung Hsing University Taichung Taiwan

In this study, the full-length VP2 gene of canine parvovirus type 2 (CPV-2) was cloned into the pBacSC vector which possesses baculovirus transmembrane domain (gp64 TM) gene, baculovirus cytoplasmic domain (gp64 CTD) gene, and green florescence protein (GFP) gene. Baculovirus gp64 TM and gp64 CTD in the pBacSC vector were designed to display heterologous proteins on the baculovirus envelope. After cloning the VP2 gene of CPV-2 into pBacSC vector, the recombinant plasmid pBacSC-VP2 was transformed into E. coli DH10Bac competent cells to form recombinant bacmid DNA. One recombinant baculovirus BacSC-VP2 that expresses the VP2 protein of CPV-2 was obtained. Confocal microscopy and immunogold electron microscopy were used to verify whether VP2 expressing on baculovirus envelope or cell membrane. Immunization of BALB/c mice with recombinant baculovirus BacSC-VP2, demonstrated that serum from the BacSC-VP2 treated models had higher levels of virus neutralization titers than the control groups. The results show that the recombinant baculovirus BacSC-VP2 can induce a strong immune response in a mouse model, suggesting that the pseudotyped baculovirus BacSC-VP2 can serve as a potential vaccine against CPV infections.

关键词： Canine Parvovirus Type 2 VP2 Protein Baculovirus gp64 TM and CTD Subunit Vaccine

平滑肌瘤女性患者中肿瘤坏死子-α-308启动子和p53密码子72基因的多态性

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世界核心医学期刊文摘（妇产科学分册） 2005年第4期1卷 33-34页

作者： Hsiehy.-y. tsai F.-J. 刘淑娟

Tumor necrosis factor-α(TNF-α), a proinflammatory cytokine, plays an important role in the process of autoimmune diseases. p53 is related to the regulation of cell growth and prevention of carcinogenesis. We propose to investigate whether gene polymorphisms for TNF-α-308 promoter and p53 could be used as markers of susceptibility in leiomyomas. Prospective basic study. Departments of gynecology and genetics in a medical center. Group 1: leiomyoma (n = 159); group 2: nonleiomyoma (n = 131). Genomic DNA was obtained from peripheral leukocyte. The TNF-αand p53 gene polymorphisms were amplified by polymerase chain reaction (PCR), enzyme restriction, and electrophoresis. Two gene polymorphisms were identified: [1] the A (cuttable)/G (uncuttable) polymorphisms of the TNF-αgene on chromosome 6p21.3; [2] A (cuttable)/P (uncuttable) polymorphisms of the p53 gene on chromosome 17p. Genotype and allelic frequencies in both groups were compared. Genotype distribution and allele frequency of TNF-α.gene polymorphism in both groups were significantly different. Proportions of A homozygote/heterozygote/G homozygote for TNF-α.in both groups were: (group 1) 61%/34.6%/4.4%and (group 2) 81.7%/14.5%/3.8%. Proportions of allele A/G for TNF-αin both groups were: (group 1) 78.3%/21.7%and (group 2) 88.9%/11.1%. Distributions of p53 polymorphisms in both groups were not different. The proportions of A homozygotes/heterozygotes/P homozygotes for p53 were (group 1) 32.7%/42.1%/25.2%and (group 2) 28.2%/48.9%/22.9%. G homozygote and G allele for TNF-αpromoter are related to a higher risk of leiomyomas. The p53 codon 72 gene polymorphism is not associated with the susceptibility of leiomyomas.

关键词：平滑肌瘤 p53 密码子启动子多态性聚合酶链反应限制性酶切纯合子自身免疫性疾病遗传学科

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铁线莲属研究随记(Ⅳ)

植物分类学报 2001年第1期39卷 1-19页

作者：王文采中国科学院植物研究所系统与进化植物学开放研究实验室北京100093

(1 )Eichler (1 958)在研究马来西亚铁线莲属植物时也深入地研究了中国、越南、泰国、缅甸及喜马拉雅山区的该属标本 ,认为根据我国台湾标本描述的ClematistaiwanianaHayata可能为C .javanaDC .的异名。本文作者在研究了有关的南洋群岛... 详细信息

(1 )Eichler (1 958)在研究马来西亚铁线莲属植物时也深入地研究了中国、越南、泰国、缅甸及喜马拉雅山区的该属标本 ,认为根据我国台湾标本描述的ClematistaiwanianaHayata可能为C .javanaDC .的异名。本文作者在研究了有关的南洋群岛、中国台湾及日本琉球群岛的标本之后同意Eichler的归并意见 ;此外 ,C .***,C .sikkimensis(Hook .f.&Thoms.)Drumm .exBurkill,C .connataDC .*** .y .Fang ,C .kilungensisW .T .Wang&M .y .Fang等拉丁学名也被归并为异名。 (2 )对大叶铁线莲组***做出了新的分类系统。特产日本的ClematistakedanaMakino自发表后一直被认为是C .apiifoliaDC .和C .stansSieb .&Zucc .(大叶铁线莲C .heracleifoliaDC .的近缘种之一 )的杂交种。此种为木质藤本植物 ,具三出复叶 ,腋生花序有多数两性花 ,呈圆锥状 ,而相似于C .apiifoliaDC .(萼片水平开展 ,雄蕊无毛 ,花药狭长圆形 ,药隔顶端不突出 ) ,但其萼片向斜上方开展 (花萼宽钟状 ) ,花丝上部有疏柔毛 ,花药条形 ,药隔顶端有短突起而不同。笔者发现 ,特产我国河北西部和北京山地的羽叶铁线莲C .pinnataMaxim .(叶为具 5小叶的羽状复叶 )具有与C .takedanaMakino相同的花构造。

关键词：铁线莲属大叶铁线莲组分类归新分类新组合新等级毛莨科

Development of the Photon Beam Position Monitors for the TLS

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Development of the Photon Beam Position Monitors for the TLS

The Second Asian Particle Accelerator Conference

作者： G.y.Hsiung Z.D.tsai T.F.Lin K.M.Hsiao S.N.Hsu J.R.Chen Synchrotron Radiation Research Center TaiwanChina Synchrotron Radiation Research Center TaiwanChina Synchrotron Radiation Research Center TaiwanChina Synchrotron Radiation Research Center TaiwanChina Synchrotron Radiation Research Center TaiwanChina Synchrotron Radiation Research Center TaiwanChina

＜正＞ Several types of the non-destructive photon beam position monitors（PBPM）are developedfor the Taiwan Light Source（TLS）in *** PBPM,installed in the front ends,measures the photon beam positions by photoemission current on the *** 2-bladetype of PBPM,measuring the vertical position of photons emitted from both bending magnetand insertion device,reveals a resolution better than 0.5 μ*** PBPM of multi-blade,

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台湾东部耐多药结核病的分子流行病学研究

国际结核病与肺部疾病杂志 2011年第1期6卷 42-45页

作者： A-H.Hsu y-S.Lee y-S.tsai J-J.Lee 黄海荣 Department of Internal Medicine Buddhist Tzu Chi General Hospital and Tzu Chi UniversityHualien Institute of Medical Science Tzu Chi UniversityHualien Sixth Branch Office Centers for Disease Control TaiwanHualien Institute of Public Health Tzu Chi UniversityHualienTaiwan Department of Laboratory Medicine Buddhist Tzu Chi General Hospital and Tzu Chi UniversityHualien

台湾从2007年5月启动了耐多药结核病项目。在台湾东部,自2007年5月到2009年2月共纳入了77例耐多药结核病(MDR-TB)患者。获取了其中的73例(93%)患者的分离株以应用间隔区寡核苷酸基因分型(spoligotyping)和MIRU-VNTR(分枝杆菌分散重复单... 详细信息

台湾从2007年5月启动了耐多药结核病项目。在台湾东部,自2007年5月到2009年2月共纳入了77例耐多药结核病(MDR-TB)患者。获取了其中的73例(93%)患者的分离株以应用间隔区寡核苷酸基因分型(spoligotyping)和MIRU-VNTR(分枝杆菌分散重复单位-可变数目的串联重复序列)进行基因分型。spoligotyping的分析结果表明,北京基因型是主要的流行基因型(n=48,66%)。在73株菌株中,28(38.4%)株有独特的基因型,45(61.6%)株菌株成簇。在45位基因型成簇的患者中,有21例患者发现存在流行病学联系。在台湾东部MDR结核病患者的菌株成簇比例高。

关键词：耐多药结核病间隔区寡核苷酸基因分型传播 MIRU-VNTR