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Phyton-International Journal of Experimental Botany 2023年第9期92卷 2489-2501页

作者： Abraham Lamboro songnan yang Xueying Li Dan Yao Jun Zhang College of Agronomy Jilin Agricultural UniversityChangchun130118China College of Life Science Jilin Agricultural UniversityChangchun130118China Department of Biology College of Natural and Computational ScienceWachemo UniversityHossana667Ethiopia

The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut cultivars.The culture media contained 4 mg/L 6-benzylaminopurine(BAP)as the main growth regulator.Various concentrations(100–600 mg/L)of cefotaxime,carbenicillin,and timentin were applied in the culture media.In all the tested media,there were no significant differences in the shoot induction as compared to the control.However,little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation media.Under shoot elongation medium,shoots turned brownish and partly died at higher concentrations where shooting rates were not affected by the treatments.In cefotaxime,timentin,and carbenicillin-containing media,levels of antibiotics greater than 400,300,and 200 mg/L,respectively resulted in the brown coloration of plantlets.Moreover,the mean shoot number and shoot weight significantly decreased as their dosage increased.The results indicate that maximum levels of antibiotics have an adverse effect on the growth and development of peanuts.Also,cefotaxime(100–300 mg/L)and timentin(100–300 mg/L)will be sufficient in controlling Agrobacterium growth in the culture media with the least phytotoxic effect on the peanut plants.

关键词： Peanut antibiotics shoot cotyledonary node regeneration

Quantitative Trait Loci(QTL)Mapping and Marker Analysis of Fatty Acids in Peanut

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Phyton-International Journal of Experimental Botany 2023年第9期92卷 2577-2589页

作者： Xiao Han songnan yang Xueying Li Qiulin Wu Yongyi Xing Jun Zhang Fenglou Ling College of Agronomy Jilin Agricultural UniversityChangchun130118China

Peanut,with high oil content,has been a major oil and food crop globally.The compositions of the fatty acids are the common factors in determining the oil quality.In the present study,an F2 segregated population with 140 individuals derived from the cross of Weihua8(a cultivar)and 12L49(a line with high oleic acid concentration)was used to construct a genetic map and conduct QTL mapping analysis.A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR loci.Within the 103 SSR loci,a genetic linkage map,covering a total length of 3592.35 cM of the whole peanut genome,was constructed.Based on the genetic map,sixteen QTLs located on nine linkage groups related to peanut fatty acids were finally identified.Among them,four QTLs were detected associated with various traits simultaneously,which showed genetic stability in relation to fatty acids of peanut.Except for the QTLs for oleic acid,linoleic acid,and linolenic acid,three novel QTLs for arachidic acid and behenic acid were also detected.These QTLs might be helpful for further fine mapping analysis and marker-assisted selection of fatty acids in peanut.

关键词： Peanut fatty acids simple sequence repeat genetic linkage map QTL mapping

Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars

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Phyton-International Journal of Experimental Botany 2022年第12期91卷 2619-2631页

作者： Abraham Lamboro Xiao Han songnan yang Xueying Li Dan Yao Baixing Song Jun Zhang Department of Crop Genetics and Breeding College of AgronomyJilin Agricultural UniversityChangchun130118China Department of Biochemistry and Molecular Biology College of Life ScienceJilin Agricultural UniversityChangchun130118China

Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits.A combination of 6-benzylaminopurine(BAP)and thidiazuron(TDZ)plant growth regulators was applied to evaluate shoot regeneration capacity whereasα-naphthalene acetic acid(NAA)combination with 6-benzylaminopurine(BAP),and 2,4-dichlorophenoxyacetic acid(2,4-D)with 6-benzylaminopurine were tested to optimize root induction for two peanut cultivars.The result showed combination(BAP with TDZ)was found to be effective in promoting shoot.The highest shoot regeneration frequency(93%)was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L TDZ while an average regeneration frequency(87%)was achieved in a medium containing combinations of 2 mg/L BAP with 1 mg/L TDZ.The shooting rate increased for both cultivars as the concentrations of BAP increased and TDZ decreased.The highest rooting rate(93%)was obtained on a medium supplemented with 3.5 mg/L NAA with 2.5 mg/L BAP for both cultivars.The rooting rate increased as the concentration of auxin to cytokinin ratio increased.The maximum rooting rate(83%)was obtained on MS medium supplemented with 0.3 mg/L 2,4-D with 0.2 mg/L BAP for the cultivar N3.The result indicated that BAP with NAA was much better than BAP with 2,4-D in rooting rate.Thus,the protocol developed was genotype independent and effective for peanut tissue culture.

关键词： Auxin cotyledonary node cytokinins in vitro peanut

Emerging applications of nanofibers electrospun from carbohydrate polymers

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Journal of Future Foods 2024年第4期4卷 289-299页

作者： Nicole Angel songnan Li Lingyan Kong Deparment of Mechanical Engineering.The University of Alabama TuscaloosaAL 35487USA Joint Intemnarional Research Laboratory of Agriculture and Agni-Product Saferty of the Ministry of Education of China Institutes of Agricultural Science and Techmology DevelopmentYangzhou UniversityYangzhou 225009China Deparment of Human Nutrition and Hospitality Management The University of AlabamaTuscaloosaAI 35487USA

Electrospinning is a simple and versatile technique that uses electrostatic forces to create fbers in the nano o micro range from a variety of materials,both synthetic and natural Due to the high surface area to volume ratio,high porosity,and desirable mechanic characteristics of electrospun fbers,they are of current interest for a wide variety of applications.Some of the most signifcant applications of these fbers being researched include tissue engineering.drug delivery,wound dressings,environmental and energy applications,and protective materials.Notably,electrospun fbers may be specially tailored to better ft their fnal application through the direct 1oading of materials during the spining process as well as by choosing the correct base material for the fber.For example,it is desirable to use a biocompatible and biodegradable material in fibers desired for applications in the biomedical field;this way the fbers are able to safely interact with the human body.This review will explore the applications,as previously listed,with a focus on how fbers are made using carbohydrate polymers(such as alginate,cellulose and its derivatives,chitosan and chitin,starch,pul1ulan,hyaluronic acid,dextran,and 1evan)as their base material,and their applicability and functionality in various applications.

关键词： Electrospinning Tissue engineering Drug delivery Wound dresing Envionment Energy Protective fabric

Natural variation and selection in GmSWEET39 affect soybean seed oil content

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Natural variation and selection in GmSWEET39 affect soybean ...

Oral anti-coagulants use in Chinese hospitalized patients with atrial fibrillation

江苏省遗传学会2019年学术研讨会

作者：苗龙 songnan yang Kai Zhang Jianbo He Chunhua Wu Yanhua Ren Junyi Gai 李艳 Nanjing Agricultural University

Soybean is recognized as the largest oilseed crop that contributes to about 61% of the world oilseed production and almost 28% of the total global vegetable oil consumption in 2018. The seed oil content has been increased during soybean domestication and improvement. However, the genes underlying the selection are largely unknown. In this study, we analyzed the expression patterns of genes in the seed oil quantitative trait loci with strong selective sweep signals, then used association, functional study and population genetics to reveal a sucrose efflux transporter gene, GmSWEET39, controlling soybean seed oil content and under selection. GmSWEET39 is highly expressed in soybean seeds and encodes a plasma membrane-localized protein. The relative expression level of GmSWEET39 is significantly correlated with soybean seed oil content. Among cultivated soybean, the sequence variation in the promoter(Pro) and coding region(CDS) of GmSWEET39 defines three types Pro and two types CDS. The seed oil content of soybean varieties with GmSWEET39 is significantly higher than that of GmSWEET39 or GmSWEET39, and seed oil content of GmSWEET39 is higher than GmSWEET39, suggesting that Pro3 and CDS2 are the potential superior alleles of GmSWEET39 that might improve soybean seed oil content. The frequencies of these potential superior alleles of GmSWEET39 in landraces are much lower than those in released cultivars. GmSWEET39 allelic effects on total oil content were confirmed in transgenic Arabidopsis, transgenic soybean hairy roots and soybean recombinant inbred lines. In comparison to control, the GmSWEET39 overexpressing Arabidopsis had significantly higher total fatty acid content in mature seeds. Also the total fatty acid content in soybean hairy roots of 35 S : GmSWEET39 and GmSWEET39:GmSWEET39 was significantly higher than that in the roots containing empty vector. Among soybean RIL population, lines carrying the Pro3 or CDS2 type of GmSWEET39 had significantly higher seed oil content than those with the Pro1 or CDS1 type

关键词： genetic improvement GmSWEET39 natural variation seed oil content soybean

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Chinese Medical Journal 2024年第2期137卷 172-180页

作者： Jing Lin Deyong Long Chenxi Jiang Caihua Sang Ribo Tang songnan Li Wei Wang Xueyuan Guo Man Ning Zhaoqing Sun Na yang Yongchen Hao Jun Liu Jing Liu Xin Du Louise Morgan Gregg C.Fonarow Sidney C.Smith Jr Gregory Y.H.Lip Dong Zhao Jianzeng Dong Changsheng Ma On behalf of CCC-AF investigators Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China National Clinical Research Center for Cardiovascular Diseases Beijing 100029 China Department of Epidemiology Beijing Anzhen HospitalCapital Medical UniversityBeijing Institute of HeartLung and Blood Vessel DiseasesBeijing 100029China International Quality Improvement Department American Heart AssociationDallasTX 07076USA Division of Cardiology Geffen School of Medicine at University of CaliforniaLos Angeles CA 90024USA Division of Cardiology University of North CarolinaChapel HillNC 27599USA Department of Cardiology Liverpool Centre for Cardiovascular ScienceUniversity of Liverpool and Liverpool Heart&Chest HospitalLiverpool L143PEUK Department of Clinical Medicine Aalborg UniversityAalborg 9000Denmark 不详

Background:Oral anti-coagulants(OAC)are the intervention for the prevention of stroke,which consistently improve clinical outcomes and survival among patients with atrial fibrillation(AF).The main purpose of this study is to identify problems in OAC utilization among hospitalized patients with AF in China.Methods:Using data from the Improving Care for Cardiovascular Disease in China-Atrial Fibrillation(CCC-AF)registry,guideline-recommended OAC use in eligible patients was assessed.Results:A total of 52,530 patients with non-valvular AF were enrolled from February 2015 to December 2019,of whom 38,203 were at a high risk of stroke,9717 were at a moderate risk,and 4610 were at a low risk.On admission,only 20.0%(6075/30,420)of patients with a diagnosed AF and a high risk of stroke were taking OAC.The use of pre-hospital OAC on admission was associated with a lower risk of new-onset ischemic stroke/transient ischemic attack among the diagnosed AF population(adjusted odds ratio:0.54,95%confidence interval:0.43-0.68;P70%of AF patients were at a high risk of stroke.Although poor performance on guideline-recommended OAC use was found in this study,over time the CCC-AF project has made progress in stroke prevention in the Chinese AF population.Registration:ClinicalTrials.gov,NCT02309398.

关键词： Atrial fibrillation Oral anti-coagulants OAC Stroke prevention Contraindication

基于系统动力学模型的深圳市龙华新区水资源供需平衡预测及优化

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应用基础与工程科学学报 2017年第5期25卷 917-931页

作者：李天宏杨松楠北京大学环境科学与工程学院水沙科学教育部重点实验室北京100871

为解决深圳市龙华新区水资源供需矛盾,运用系统动力学模型(SD模型),结合该区"来-蓄-用-排"的水资源结构链,将人口、经济、产业发展与水资源供需相结合,建立水资源SD模型,模拟了2010~2030年水资源供需比的变化.结果显示,未来在限定境外... 详细信息

为解决深圳市龙华新区水资源供需矛盾,运用系统动力学模型(SD模型),结合该区"来-蓄-用-排"的水资源结构链,将人口、经济、产业发展与水资源供需相结合,建立水资源SD模型,模拟了2010~2030年水资源供需比的变化.结果显示,未来在限定境外调水总量的前提下龙华新区水资源匮乏,供需矛盾突出.通过参数敏感性分析识别出6个对水资源供需比影响较大的参数,分别是污水处理回用率、雨水利用率、人均生活用水定额、万元工业产值用水量、第二产业增长率、第三产业增长率.将以上参数作为主要调控变量,依据龙华新区发展规划及国家发展战略设计了情景优化方案,最终给出短期规划与长期规划综合优化方案.短期规划到2020年水资源供需比可由0.93提高到1.05,长期规划到2030年水资源供需比可由0.84提高到1.04.未来龙华新区需进一步加快产业结构调整,建设节水型城市,提高非常规水资源利用率,才能有效解决水资源供需矛盾,保持经济稳增与水资源可持续发展的协调统一.

关键词：深圳市龙华新区水资源系统动力学水资源供需平衡

预应力简支梁桥在移动荷载作用下的非线性动力特性分析

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土木工程学报 2009年第1期42卷 49-54页

作者：罗松南杨敬林湖南大学湖南长沙410082

考虑预应力梁的剪切变形和几何非线性,建立梁的动力学基本方程式,应用增量谐波平衡法,分析计算梁在预应力作用下的横向振动问题。通过实例计算,得到梁在移动车辆荷载作用下的幅频响应曲线和力与位移的非线性响应关系,并给出了外激励频... 详细信息

考虑预应力梁的剪切变形和几何非线性,建立梁的动力学基本方程式,应用增量谐波平衡法,分析计算梁在预应力作用下的横向振动问题。通过实例计算,得到梁在移动车辆荷载作用下的幅频响应曲线和力与位移的非线性响应关系,并给出了外激励频率不同且初始预加力变化时动挠度的变化规律。计算结果表明,梁非线性位移明显大于线性位移,且荷载与位移呈明显非线性关系,非线性因素的影响不可忽略;施加预应力可以提高梁的动抗弯刚度,减少梁的动挠度值。为预应力混凝土桥梁的设计和动力特性分析提供一定的理论依据。

关键词：预应力桥移动荷载非线性分析动力响应

基于差分Causal LSTM模型的气象图像短时预测研究

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西安理工大学学报 2023年第4期39卷 529-535页

作者：张晓晖白文奇杨松楠王晓娟西安理工大学自动化与信息工程学院陕西西安710048

为解决气象图像序列在短时预测时预测精度低的问题,利用一种具有级联记忆单元的Causal LSTM,将图像梯度差分惩罚因子引入训练过程,来提高预测模型对短时序列动态和突变的建模能力,提出了差分Causal LSTM模型。研究首先通过循环神经网络... 详细信息

为解决气象图像序列在短时预测时预测精度低的问题,利用一种具有级联记忆单元的Causal LSTM,将图像梯度差分惩罚因子引入训练过程,来提高预测模型对短时序列动态和突变的建模能力,提出了差分Causal LSTM模型。研究首先通过循环神经网络建立气象图像短时预测模型,然后分析了ConvLSTM模型对气象雷达回波图与卫星云图序列的预测效果,对于ConvLSTM模型预测气象图像存在严重模糊的问题,使用差分Causal LSTM模型进行优化,结果表明改进的模型能够有效改善模糊,提升预测结果的准确性。改进后的差分Causal LSTM模型在HKO-7数据集的测试样本中,关键成功指数(CSI)提高了0.019,在气象云图数据集中提高了0.078,模糊程度有所减弱。

关键词： ConvLSTM Causal LSTM 端到端模型图像梯度差分损失

不同叶面肥在不同时期喷施对安徽沿江地区小麦产量及其构成因素的影响

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现代农业研究 2023年第9期29卷 49-53页

作者：江忠周文杨松楠陈远方天宇安徽省华阳河农场农科所安徽安庆246531

为探索掌握安徽沿江地区华阳河农场农科所新引进不同叶面肥在小麦不同时期喷施的效果,进一步鉴定不同叶面肥对小麦生长及主要性状构成因素和产量的影响变化规律,同时鉴定不同叶面肥在安徽沿江地区小麦生产上是否有利用价值,为安徽沿江... 详细信息

为探索掌握安徽沿江地区华阳河农场农科所新引进不同叶面肥在小麦不同时期喷施的效果,进一步鉴定不同叶面肥对小麦生长及主要性状构成因素和产量的影响变化规律,同时鉴定不同叶面肥在安徽沿江地区小麦生产上是否有利用价值,为安徽沿江地区小麦生产上推广应用提供科学依据。通过此项试验研究结果表明:叶面肥“新美洲星”和“津平1号”在安徽沿江地区小麦上喷施效果明显,能增加小麦单穗粒数和千粒重,且增产幅度较大,适应安徽沿江地区小麦生产推广应用。

关键词：不同叶面肥不同时期喷施安徽沿江地区小麦产量构成因素影响