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Evolutionary study of hepatitis C virus envelope genes during primary infection

Chinese Medical Journal 2007年第24期120卷 2174-2180页

作者： CHEN song WANG yu-ming Department of Infectious Diseases Southwest Hospital Third Military Medical University Chongqing 400038 China

Background Hepatitis C virus （HCV） envelope genes encoding glycoproteins E1 and E2 exhibits a high degree of variability that gives rise to differing phenotypic traits; including alterations in receptor-binding affinity and immune recognition and escape. This study aims to elucidate the relationship of the evolutionary patterns for HCV envelope glycoproteins to viral persistence. Methods HCV quasispecies were characterized in specimens collected every two to six months from a cohort of acutely HCV-infected subjects. We evaluated two individuals who spontaneously cleared viremia and three individuals with persistent viremia by cloning 33 1-kb amplicons that spanned E1 and the 5＇ half of E2; including hypervariable region 1 （HVR1）. To detect representative variants for sequencing thirty-three cloned cDNAs representing each specimen were assessed by a method that combined analysis of a single-stranded conformational polymorphism （SSCP） method and heteroduplex analysis （HDA）. For each patient, the rates of both synonymous and nonsynonymous substitutions for the El, HVR1 and E2 regions outside HVR1 were evaluated. The amino acid sequences and predicted antigenic profiles were analyzed. Results The genetic diversity within HVR1 was consistently higher than that in the E1 and E2 regions outside HVR1 in individuals with persistent viremia, but did not change markedly over time in those with clearance of viremia. For individuals with persistent viremia, the rate of nonsynonymous substitutions within the HVR1 region predominated and gradually increased, compared to that in the E1 and E2 regions outside HVR1. By contrast, the rates of both nonsynonymous and synonymous substitutions for the E1 and E2 regions, including HVR1, were consistently lower in individuals with clearance of viremia. HVR1 had a higher antigenic variable and lower positive charge in subjects with persistent viremia. All cysteine residues and N-linked glycosylation sites, some of which were known to play

关键词： hepatitis C virus quasispecies envelope population dynamics

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Poly(vinylidene fluoride)/Clay Nanocomposites by Melt Compounding

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Chemical Research in Chinese Universities 2008年第1期24卷 116-119页

作者： song yu-ming ZHAO Zhu-di yu Wen-xue ZHENG Wei-tao LI Bo YANG Xiao-wei CHEN Xin-fang School of Materials Science and Engineering Key Lab of Automobile Materials of MOE Jilin University Changchun 130012 P. R. China China-Japan Union Hospital of Jilin University Changchun 130031 P. R. China Solvay（Shanghai） Co. Ltd. Shanghai 200131 P. R. China

The crystalline structures, morphologies, and mechanical properties of poly（vinylidene fluoride）/clay nanocomposites were studied using X-ray diffraction（XRD）, transmission electron microscopy（TEM）, Fourier transform infrared spectroscopy（FTIR）, polarized optical microscopy（POM）, and tensile tests. The results of XRD and TEM show that organoclays are dispersed in the poly（vinylidene fluoride）（PVDF） matrix. A clay-induced crystal transformation from α-phase to β-phase of PVFD was confirmed by XRD and FTIR. Clay layers restricted the growth of spherulite. The tensile tests indicate that the tensile modulus and yield strength as well as the elongation at break decrease when clay is loaded.

关键词： Poly(vinylidene fluoride) Nanocomposite Crystalline structure Morphology Mechanical property

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Research on individual motivation model of software engineering

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通讯和计算机（中英文版） 2009年第11期6卷 12-16页

作者： song yu WANG ming North China Electric Power University Baoding 071003 China

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Inhibition of Ampicillin-resistance in Bacteria by Modified DNAzymes

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Chemical Research in Chinese Universities 2008年第4期24卷 491-495页

作者： CHEN Fei LI Zhe YANG Shuo WANG Rui-jian LIU Bin song yu-ming SUN Yan-hong HAO Dong-yun WANG Xiao-ping Key Lab for Molecular Enzymology and Engineering of the Ministry of Education Jilin University Changchun 130021 P. R. China China-Japan Friendship Hospital Jilin University Changchun 130031 P. R. China

To overcome ampicillin-resistance of bacteria which is believed to attribute their endogenous B-lactamase, we designed three 10-23 DNAzymes（Dz1, Dz2. Dz3） targeting the coding region of B-lactamase mRNA and examined their inhibitory capabilities of the ampicillin-resistance of TEM-1 and TEM-3 bacteria. Dz1 was a traditional 10-23 DNAzyme, Dz2 was the mutant of Dz1 by addition of the protected nucleotide to each ann of the enzyme, and Dz3 was a mutant of Dz1 at antisense arms of which phosphorothioate modifications were made. Kinetic analysis, bacterial growth, and β-lactamase activity measurement showed that all the three DNAzymes worked efficiently in vitro and in vivo. A 9 hours bacterial growth inhibition test showed that the inhibition rates of TEM-1 bacteria by Dz1, Dz2, and Dz3 were 27%, 50%, and 29%, respectively. In addition, the inhibition rates of TEM-3 bacteria by those three DNAzymes were found io be 49%, 58%, and 45%, respectively. The current findings suggest that DNAzymes may become potential candidates of alternative inhibitors for bacteria drug-resistance.

关键词： DNAzyme Drug-resistance β-Lactamase Ampicillin Antisense drug

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Multilevel analysis of the central-peripheral-target organ pathway:contributing to recovery after peripheral nerve injury

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Neural Regeneration Research 2025年第10期20卷 2807-2822页

作者： Xizi song Ruixin Li Xiaolei Chu Qi Li Ruihua Li Qingwen Li Kai-yu Tong Xiaosong Gu Dong ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Haihe Laboratory of Brain-Machine Interface and Human-Machine Fusion TianjinChina Department of Rehabilitation Tianjin University Tianjin HospitalTianjinChina Department of Hand Microsurgery Tianjin University Tianjin HospitalTianjinChina School of Exercise and Health Tianjin University of SportTianjinChina Department of Biomedical Engineering the Chinese University of Hong KongHong Kong Special Administrative RegionChina

Peripheral nerve injury is a common neurological condition that often leads to severe functional limitations and *** on the pathogenesis of peripheral nerve injury has focused on pathological changes at individual injury sites,neglecting multilevel pathological analysis of the overall nervous system and target *** has led to restrictions on current therapeutic *** this paper,we first summarize the potential mechanisms of peripheral nerve injury from a holistic perspective,covering the central nervous system,peripheral nervous system,and target *** peripheral nerve injury,the cortical plasticity of the brain is altered due to damage to and regeneration of peripheral nerves;changes such as neuronal apoptosis and axonal demyelination occur in the spinal *** nerve will undergo axonal regeneration,activation of Schwann cells,inflammatory response,and vascular system regeneration at the injury *** damage to target organs can occur,including skeletal muscle atrophy and sensory receptor *** then provide a brief review of the research advances in therapeutic approaches to peripheral nerve *** main current treatments are conducted passively and include physical factor rehabilitation,pharmacological treatments,cell-based therapies,and physical ***,most treatments only partially address the problem and cannot complete the systematic recovery of the entire central nervous system-peripheral nervous system-target organ ***,we should further explore multilevel treatment options that produce effective,long-lasting results,perhaps requiring a combination of passive(traditional)and active(novel)treatment methods to stimulate rehabilitation at the central-peripheral-target organ levels to achieve better functional recovery.

关键词： central nervous system central peripheral target organ multilevel pathological analysis nerve regeneration peripheral nerve injury peripheral nervous system target organs therapeutic approach

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Genetic evolution of structural region of hepatitis C virus in primary infection

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World Journal of Gastroenterology 2002年第4期8卷 686-693页

作者： song Chen yu-ming Wang Department of Infectious Diseases,Southwest Hospital,Third Military Medical University,Chongqing 400038,China Department of Infectious Diseases Southwest Hospital Third Military Medical University30 Gaotanyan Zhengjie Shapingba DistrictChongqing 400038 Chin

AIM：To investigate the dynamics of hepatitis Cirus(HCV)variability through putative envelope genes during primary infection and the mechanism of viral genetic evolution in infected ***:Serial serum samples prospectively collected for12to34months from a cohort of acutely HCV-infected individuals were obtained,and a 1-kb fragment spanning E1and the5′half of e2,including Thirty-three cloned cDNAs representing each specimen were assessed by a method that combined a single-stranded conformational polymorphism(SSCP)and heteroduplex analysis(HDA)method to determine the number of clonotypes hypervariable region,was amplified by reverse transcriptase PCRand *** mutations per nonsynonymous site(dn),synonymous mutations per synonymous site(ds),dn/ds ratio and genetic distances within each sample were evaluated for intrahost evolutionary ***:Quasispecies complexity and sequence diversity were lowen in early samples and a further increase after serocon version,although ds value in the envelope genes was higher than dn value during primary *** trend,pronounced in most of samples,toward lower ds values in the E1 than in the 5′portion of *** complexity was higher and E2dn/ds ratio was a trend toward higher value in later asmples during persistent viremia,We also found individual features of HCV genetic evolution in different subjects who were infected with different HCV ***:Mutations of actively replicating virus arise stochastically with certain functional *** quasispecies exerted by a combination of either neutral evolution or selective forces shouws clear differences in individuals,and associated with HCV persistence.

关键词：初次感染丙型肝炎病毒遗传演变病毒结构

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正交试验优化燕麦营养冰淇淋工艺

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粮食与油脂 2018年第9期31卷 47-50页

作者：宋宇鸣青岛酒店管理职业技术学院山东青岛266100

主要研究燕麦冰淇淋的生产工艺及产品配方,探讨燕麦粉、全脂奶粉、明胶、单甘酯添加量对冰淇淋品质的影响。采用单因素试验与正交试验,筛选燕麦冰淇淋的最佳生产配方。结果表明:当燕麦粉添加量为4%,全脂奶粉添加量为10%,明胶添加量为0.... 详细信息

主要研究燕麦冰淇淋的生产工艺及产品配方,探讨燕麦粉、全脂奶粉、明胶、单甘酯添加量对冰淇淋品质的影响。采用单因素试验与正交试验,筛选燕麦冰淇淋的最佳生产配方。结果表明:当燕麦粉添加量为4%,全脂奶粉添加量为10%,明胶添加量为0.4%,单甘酯添加量为0.4%时,得到的产品组织状态良好,口感细腻,具有浓郁的麦香味和较高的营养价值。

关键词：燕麦冰淇淋

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： ming‑yuan yu Jing Wu Guang Yin Fan‑Zhen Jiao Zhong‑Zhen yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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Effects of rare earth element lanthanum on the microstructure of copper matrix diamond tool materials

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Rare Metals 2002年第2期21卷 90-94页

作者： JIAChengchang songyueqing yuming WANGTing ResearchInstituteofPowderMetallurgyuniversityofScienceandTechnologyBeijing Beijing100083China GeneralResearchInstituteofNon-ferrousMetal Beijing100088China

Effects of rare earth element La on the microstructure of Cumatrix diamond tools were researched under the conditions of variousmaterials components and the process parameters in order to improvematerials properties. SEM, XPS and X-ray were used to investigate thefracture section, microstructure and the element valence inmaterials. The Results shown that the combination of rare earthelement La and transition element Ti is advantageous to the bondingstate Between diamond particles and matrix, so it can improve thematerials properties. Suitable sintering temperature is 790 deg. C.

关键词： rare earth element La interface reaction Copper matrix diamond tool titanium

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政务微博发展状况浅析——以南京政务微博为例

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江苏师范大学学报（哲学社会科学版） 2014年第2期40卷 154-159页

作者：于松明南京晓庄学院新闻传播学院江苏南京210017

信息时代,网络媒介获得了空前的发展,以微博为代表的社交网络使得信息发布的成本大大降低,发送方式越发便捷、传播速度愈加迅速。如何借助微博扩大官方声音,防止信息在传播过程中失真,制止谣言肆意传播,提高政府公信力,推动社会主义民... 详细信息

信息时代,网络媒介获得了空前的发展,以微博为代表的社交网络使得信息发布的成本大大降低,发送方式越发便捷、传播速度愈加迅速。如何借助微博扩大官方声音,防止信息在传播过程中失真,制止谣言肆意传播,提高政府公信力,推动社会主义民主政治的建设和发展,已成为政务信息管理工作中不可忽视的一个重要内容。南京地区较早将政务微博用于工作实践当中,通过对其部分政务微博的简要分析,总结出当前政务微博运行中的可取之处以及存在的不足,并在此基础上对未来政务微博的发展趋势进行预测,为政府机关在电子政务建设中提供参考和帮助,推动政府管理方式改革,更好地建设服务型政府。

关键词：政务微博运行状况信息管理发展趋势

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