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Identification and characterization of the chalkiness endosperm gene CHALK-H in rice(Oryza sativa L.)

Journal of Integrative Agriculture 2023年第10期22卷 2921-2933页

作者： PIAO Ri-hua CHEN Mo-jun MENG Fan-mei qi Chun-yan KOH Hee-jong GAO Meng-meng song An-qi JIN Yong-mei YAN Yong-feng Rice Research Institute Jilin Academy of Agricultural SciencesGongzhuling 136100P.R.China Research Institute of Agricultural Biotechnology Jilin Academy of Agricultural SciencesChangchun 130033P.R.China Department of Agriculture Forestry and BioresourcesPlant Genomics and Breeding InstituteSeoul National UniversitySeoul 08826Republic of Korea College of Agricultural Sciences Yanbian UniversityYanji 133000P.R.China

Chalkiness is one of the most important agronomic traits in rice breeding,which directly affects the quality of rice *** this study,we identified a chalkiness endosperm mutant,chalk-h,from N-methyl-N-nitrosourea(MNU)-induced japonica rice cultivar Hwacheong(HC).Compared with wild type(WT)-HC,chalk-h showed severe chalkiness in the endosperm,yellowish green leaves,as well as reduced plant *** electron microscopy(SEM)analysis showed that starch grains in the chalk-h mutant were irregular in size and loosely arranged,with large gaps between granules,forming ovoid or orbicular *** analysis revealed that the phenotype of chalk-h is controlled by a single recessive gene LOC_Os11g39670 encoding seryl-tRNA synthetase,which is renamed as CHALK-H.A point mutation occurs in chalk-h on the sixth exon(at nucleotide 791)of CHALK-H,in which adenine(A)is replaced by thymidine(T),resulting in an amino acid codon change from glutamine(Glu)to valine(Val).The chalk-h mutant exhibited a heat-sensitive phenotype from the 3-leaf stage,including yellow-green leaves and reduced pigment *** transcriptional expression of starch synthesis-related genes was down-regulated in the chalk-h mutants compared to WT-HC at different grain-filling *** an increase in temperature,the expression of photosynthesis-related genes was down-regulated in the chalk-h mutant compared to *** of CHALK-H rescued the phenotype of chalk-h,with endosperm and leaf color similar to those of *** findings reveal that CHALK-H is a causative gene controlling chalkiness and leaf color of the chalk-h ***-H is the same gene locus as TSCD11,which was reported to be involved in chloroplast development under high *** suggest that CHALK-H/TSCD11 plays important roles not only in chloroplast development,but also in photosynthesis and starch synthesis during rice growth and development,so it has great application potential in rice breeding for high qu

关键词： CHALK-H Seryl-tRNA synthetase chalkiness rice

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Exosomes derived from circBCRC-3-knockdown mesenchymal stemcells promoted macrophage polarization

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BIOCELL 2020年第4期44卷 623-629页

作者： qi song JUN ZHANG qiANG ZHANG JING LIU KE LV JIALU YAO YAFENG ZHOU Department of Cardiology Suzhou Municipal Hospital Affiliated to Nanjing Medical UniversitySuzhou215000China Department of Cardiology Dushuhu Public Hospital Affiliated to Soochow UniversitySuzhou215000China Department of Cardiology The First Affiliated Hospital of Soochow UniversitySuzhou215000China

Macrophages play an essential role in the myocardial ischemia-reperfusion injury(MIRI),and the macrophage shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of *** has been reported that miR-182 plays an important role in MSC-Exo-associated macrophage *** circBCRC-3 is a newly discovered circle RNA that worked as a sponge of miR-182,this research aimed to find if circBCRC-3 plays a role in MSC-Exo-associated macrophage ***,circBCRC-3 was identified by divergent primers in mesenchymal stem cells(MSCs).Secondly,the exosome of MSCs was isolated and identified by transmission electron microscopy(TEM),nanoparticle-tracking analysis,and western blotting *** expression level of circBCRC-3 in MSCexos was detected by ***,the polarization of the RAW264.7 cell phenotype was analyzed by flow ***,we first identified circBCRC-3 in *** results further confirmed that MSCexo could effectively shift the macrophage polarization state from M1 towards the M2 phenotype,which indicated its role in MIRI cure.

关键词： Exosome CircBCRC-3 Mesenchymal stem cell Myocardial ischemia-reperfusion injury

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Low-bias,high-photoresponsivity SnSe2 nanofilm with an Au split-ring array-based THz detector toward 6G communication

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Science China(Information Sciences) 2023年第6期66卷 311-312页

作者： qi song Zhiwen ZHOU Yifei XU Min ZHANG Junbo YANG Huawei LIANG Tianwu WANG Peiguang YAN College of Physics and Optoelectronic Engineering Shenzhen University Shandong Key Laboratory of Optical Communication Science and Technology School of Physics Science and Information TechnologyLiaocheng University College of Liberal Arts and Sciences National University of Defense Technology GBA Research Institute Aerospace Information Research Institute of Chinese Academy of Science

6G technology with the four characteristics of an intelligent endogenous,secure endogenous,multi-domain convergence,and integrated computing network,will help society toward an intelligent era [1]. The low power consumption and high photoresponsivity of 6G core devices,and particularly the improvement of detector performance,

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Upcycling of Poly(butylene adipate-co-terephthalate)into Dual Covalent Adaptable Networks through Chain Breaking-Crosslinking Strategy

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Chinese Journal of Polymer Science 2024年第10期42卷 1505-1513,I0011页

作者： Bin-Bo Wang Rong Huang Xin Wang Tao Jiang Yi Wang Shuai Du Fa-Lin Li Jin Zhu song-qi Ma Key Laboratory of Synthetic and Biological Colloids Ministry of EducationSchool of Chemical and Material EngineeringJiangnan UniversityWuxi214122China Key Laboratory of Bio-based Polymeric Materials Technology and Application of Zhejiang Province Divisions of Polymers and CompositesNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo315201China University of Chinese Academy of Sciences Beijing100049China

Poly(butylene adipate-co-terephthalate)(PBAT),a widely studied biodegradable material,has not effectively addressed the problem of plastic *** into consideration the cost-effectiveness,upcycling PBAT should take precedence over direct composting *** present work adopts a chain breaking-crosslinking strategy,upcycling PBAT into dual covalent adaptable networks(CANs).During the chainbreaking stage,the ammonolysis between PBAT and polyethyleneimine(PEI)established the primary crosslinked ***,styrene maleic anhydride copolymer(SMA)reacted with the hydroxyl group,culminating in the formation of dual covalent adaptable *** contrast to PBAT,the PBAT-dual-CANs exhibited a notable Young's modulus of 239 MPa,alongside an inherent resistance to creep and *** to catalysis from neighboring carboxyl group and excess hydroxyl groups,the PBAT-dual-CANs exhibited fast stress ***,they could be recycled through extrusion and hot-press reprocessing,while retaining their *** straightforward strategy offers a solution for dealing with plastic waste.

关键词： Poly(butylene adipate-co-terephthalate) Dynamic crosslinked networks Chain breaking-crosslinking strategy Upcycle Recycle

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Dynamic Microwave-assisted Extraction of Flavonoids from Radix Scutellariae

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Chemical Research in Chinese Universities 2007年第2期23卷 148-153页

作者： YOU Jing-yan ZHANG Hua-rong DING Lan XIAO Ting-ting ZHANG Han-qi song Da-qian College of Chemistry Jilin University Changchun 130012 P. R. China

Dynamic microwave-assisted extraction（DMAE） of flavonoids from Radix Scutellarie was described in this article. A TM010 microwave resonant cavity was used as the energy coupler. Several parameters of DMAE, including concentration, flow rate, volume of the extraction solvent, and irradiation power, were optimized. The extraction was performed under the optimum conditions for the extraction of total flavonoids. The total flavonoids were determined by UV-Vis spectrophotometry. The main bioactive components, including baicalin, baicalein, and wogonin, were determined by HPLC. The extraction yields of the tlavonoids obtained by DMAE were compared with those obtained by pressurized microwave assisted extraction （ PMAE）, ultrasonic extraction （ UE ）, soxhlet extraction （ SE ）, and reflux extraction（ RE）, and these results indicate that DMAE is a good alternative method for the extraction of flavonoids from Radix Scutellade.

关键词： DMAE Flavonoids UV-Vis spectrophotometry HPLC

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Dual inhibition of glucose uptake and energy supply synergistically restrains the growth and metastasis of breast cancer

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Chinese Chemical Letters 2023年第4期34卷 127-132页

作者： Yuan Xu Liling Huang Yuyang Bi qi song Mengmeng Zhang Lingfeng Zhang Tianjiao Zhou Lei Xing Hulin Jiang State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China Jiangsu Key Laboratory of Druggability of Biopharmaceuticals China Pharmaceutical UniversityNanjing 210009China

Metastatic breast cancer(MBC) is one of the most common and knotty diseases in female population which could place them in a life-threatening condition. For malignant proliferation and migration, cancer cells require a large amount of glucose and energy to meet the demand of rapid metabolism. Hence,efficiently diminishing the utilization of energy substances by cancer cells is emerging as validated therapeutic strategies for cancer therapy. Herein, a nanoplatform with dual-inhibition of glucose uptake and oxidative phosphorylation(OXPHOS) was designed, which consisted of albendazole(ABZ) and atovaquone(ATO) by simple carrier-free self-assembling. The introduction of ABZ could evidently decrease glucose uptake to reduce the main “energy fuel” of cancer cells. Meanwhile, as a blocker of OXPHOS, ATO would reduce adenosine triphosphate(ATP) production and ameliorate hypoxia microenvironment by suppressing mitochondrial respiratory chain. Under such dual inhibition of energy metabolism, AA NPs exerted synergistic energy exhaustion effect and outstanding hypoxia improvement function, efficiently inhibiting tumor growth and metastasis. This research not only illustrates the feasibility of energy metabolism therapy by co-inhibiting glucose uptake and OXPHOS, but also provides an ingenious tactic to diminish metastasis during MBC treatment.

关键词： Metastatic breast cancer Self-assembled nanosystem Energy exhaustion Hypoxia alleviation Synergistic therapy

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Reaction Kinetic Equation for Char Combustion of Underground Coal Gasification

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Journal of China University of Mining and Technology 2006年第2期16卷 141-146页

作者： YU Hong-guan YANG Lan-he FENG Wei-min LIU Shu-qin song Zhen-qi School of Chemical and Environmental Engineering Shandong University of Science and Technology Qingdao Shandong 266510 China School of Mineral Resource and Geoscience China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China Engineering Department Beizao Coal Mine Group Companies of Longkou Mining Industry Longkou Shangdong 265700 China School of Chemical and Environmental Engineering China University of Mining ＆ Technology Beijing 100083 China School of Mineral Resource and Environmental Engineering Shandong University of Science and Technology Qingdao Shandong 266510 China

Based on the quasi-steady-state approximation, the dynamic equation of char combustion in the oxidation zone of underground coal gasification (UCG) was derived. The parameters of the dynamic equation were determined at 900℃ using a thermo-gravimetric (TG) analyzer connected to a flue gas analyzer and this equation. The equation was simplified for specific coals, including high ash content, low ash content, and low ash fusibility ones. The results show that 1) the apparent reaction rate constant increases with an increase in volatile matter value as dry ash-free basis,2) the effective coefficient of diffusion decreases with an increase in ash as dry basis, and 3) the mass transfer coefficient is independent of coal quality on the whole. The apparent reaction rate constant, mass-transfer coefficient and effective coefficient of diffusion of six char samples range from 7.51×104 m/s to 8.98×104 m/s, 3.05×106 m/s to 3.23×106 m/s and 5.36×106 m2/s to 8.23×106 m2/s at 900℃, respectively.

关键词： underground coal gasification char combustion kinetic equation thermo-gravimetric

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Theoretical study on order–disorder phase transition of CH3NH3PbCl3

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Chinese Physics B 2019年第11期28卷 264-267页

作者： Jing Chang Zhen-Yi Jiang qi song Lei Chen Yan-Min Lin Bo Zhou Shaanxi Key Laboratory for Theoretical Physics Frontiers Institute of Modern PhysicsNorthwest UniversityXi’an 710069China Multidisciplinary Materials Research Center Frontier Institute of Science and TechnologyXi’an Jiaotong UniversityXi’an 710054China

Order–disorder phase transitions for CH3NH3PbCl3 are studied with density functional theory. Our calculations show that the disorder is manifested in two aspects in the cubic phase, namely, the disorder of orientation and rotation of organic groups. Organic groups of [CH3] and [NH3] in cubic crystals can easily rotate around its C3 axis. At the same time,[CH3NH3]^+ organic groups can also orient to different spatial directions due to the weak interactions between organic group and inorganic frame. Our results show that its possible phase transition path starts from the deviation of organic groups from the crystal c-axis. Its structural transition changes from disordered cubic phase to hydrogen-only disordered tetragonal structure in the process of decreasing symmetry. The disordered high temperature cubic phase can be expressed as a statistical average of substructures we rebuilt. The electrostatic repulsive force between adjacent organic groups triggers out the formation of low temperature phase on cooling.

关键词： order–disorder state phase transition rotation barrier dipole interaction

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Improvement of Ga_(2)O_(3)vertical Schottky barrier diode by constructing NiO/Ga_(2)O_(3)heterojunction

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Journal of Semiconductors 2024年第4期45卷 63-68页

作者： Xueqiang Ji Jinjin Wang song qi Yijie Liang Shengrun Hu Haochen Zheng Sai Zhang Jianying Yue Xiaohui qi Shan Li Zeng Liu Lei Shu Weihua Tang Peigang Li School of Integrated Circuits&State Key Laboratory of Information Photonics and Optical Communications Beijing University of Posts and TelecommunicationsBeijing 100876China College of Integrated Circuit Science and Engineering Nanjing University of Posts and TelecommunicationsNanjing 210023China Beijing Microelectronics Technology Institute Beijing 100076China

The high critical electric field strength of Ga_(2)O_(3)enables higher operating voltages and reduced switching losses in power electronic *** Schottky metals and epitaxial films are essential for further enhancing device *** this work,the fabrication of vertical Ga_(2)O_(3)barrier diodes with three different barrier metals was carried out on an n--Ga_(2)O_(3)homogeneous epitaxial film deposited on an n+-β-Ga_(2)O_(3)substrate by metal-organic chemical vapor deposition,excluding the use of edge *** ideal factor,barrier height,specific on-resistance,and breakdown voltage characteristics of all devices were investigated at room *** addition,the vertical Ga_(2)O_(3)barrier diodes achieve a higher breakdown volt-age and exhibit a reverse leakage as low as 4.82×10^(-8)A/cm^(2)by constructing a NiO/Ga_(2)O_(3)***,Ga_(2)O_(3)power detailed investigations into Schottky barrier metal and NiO/Ga_(2)O_(3)heterojunction of Ga_(2)O_(3)homogeneous epitaxial films are of great research potential in high-efficiency,high-power,and high-reliability applications.

关键词： Ga_(2)O_(3) Schottky barrier diode NiO/Ga_(2)O_(3)heterojunction

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Cuticular protein LmTwdll is involved in molt development of the migratory locust

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Insect Science 2016年第4期23卷 520-530页

作者： Tian-qi song Mei-Ling Yang Yan-Li Wang qing Liu Hui-Min Wang Jie Zhang Tao Li Research Institute of Applied Biology Shanxi University Taiyuan and State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China Beijing Institutes of Life Science Chinese Academy of Sciences Beijing China

The cuticle, an essential structure for insects, is produced from cuticular proteins and chitin via a series of biochemical reactions. Tweedle genes are important members of the cuticular protein family and have four conserved motifs binding to chitin. Tweedle family genes have been found to play a profound effect on cuticle development. Here, we report that the cuticular protein gene LmTwdll ofLocusta migratoria belongs to the Tweedle family. In situ hybridization showed that LmTwdll is localized to epidermal cells of the cuticle. The expression patterns of LmTwdll showed low expression in the cuticle during the early and middle stages of the fifth-instar nymphs; in contrast, its expression rapidly increased in the late stages of fifth-instar nymphs. We performed RNA interference to examine the function of LmTwdll in locusts. Silencing of LmTwdll resulted in high mortality during the molting process before the next stage. Also, the epicuticle of nymphs failed to molt, tended to be thinner and the arrangement of chitin in the procuticle appeared to be disordered compare to the control group. These results demonstrate that LmTwdl! plays a critical role in molting, which contributes to a better understanding of the distinct functions of the Tweedle family in locusts.

关键词： cuticular protein Lm Twdll Loeusta migratoria molting RNA interference

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