检索结果-南通市图书馆

Acta Oceanologica Sinica 2023年第4期42卷 72-80页

作者： Chenyu song Zhong Tu na song Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Shandong Fisheries Development and Resources Conservation Center Yantai 264003China

Acanthogobius ommaturus, which belongs to the family Gobiidae, is a euryhaline and demersal fish that is widely distributed in the coastal areas, harbors, and estuaries of China, D. P. R. Korea and Japan. In this study, the genetic diversity and genetic structure of five geographical populations of A. ommaturus was assessed using the mitochondrial hypervariable region gene and microsatellite markers. The results of the two genetic markers indicated that the A. ommaturus populations had a high level of genetic diversity. The mitochondrial marker detected weak genetic differentiation among populations, and the Neighbor-Joining tree showed that there was no obvious pedigree branches and geographic structure as well. However, population of Zhoushan showed significant genetic differentiation with other populations by microsatellite markers. The population of *** has not experienced bottleneck effect recently. We speculated that the Pleistocene climate change and juvenile fish dispersal played an important role in the population differentiation of A. ommaturus.

关键词： mitochondrial markers microsatellite markers genetic diversity genetic structure Acanthogobius ommaturus

马铃薯转录因子StFBH3对非生物逆境胁迫的响应分析

在线全文

学校读者我要写书评

暂无评论

作物学报 2025年第1期51卷 247-259页

作者：宋倩娜宋慧洋李京昊段永红梅超冯瑞云山西农业大学农学院山西太谷030801 山西省农业科学院作物科学研究所山西太原030031

bHLH (basic helix-loop-helix)作为植物界第二大类转录因子,在植物应对环境胁迫响应中起着重要的调控作用。探究马铃薯(Solanum tuberosum L.) bHLH家族基因功能将为马铃薯改良和育种提供一定的理论依据。本研究克隆了马铃薯StFBH3基因... 详细信息

bHLH (basic helix-loop-helix)作为植物界第二大类转录因子,在植物应对环境胁迫响应中起着重要的调控作用。探究马铃薯(Solanum tuberosum L.) bHLH家族基因功能将为马铃薯改良和育种提供一定的理论依据。本研究克隆了马铃薯StFBH3基因(Gene ID:102582309),利用qPCR技术分析了不同逆境胁迫下StFBH3基因表达模式,结果表明, StFBH3基因在马铃薯根和叶中的表达量较高,且该基因的表达受渗透、高盐和脱落酸(abscisic acid, ABA)诱导;以过表达StFBH3基因的马铃薯试管苗为材料,在分别含有不同浓度甘露醇(mannitol)、naCl和ABA的MS培养基中,过表达StFBH3马铃薯株系的叶绿素含量显著高于野生型,根长显著长于野生型。在干旱和高盐处理下,土壤栽培的过表达马铃薯株系较野生型表现较强的耐受性,叶片相对含水量、叶绿素含量和过氧化物歧化酶(SOD)活性显著高于野生型。qPCR分析发现,干旱和盐胁迫处理下相关基因(KAT1)的表达量在过表达马铃薯株系中较野生型显著降低。以上结果表明, StFBH3基因可能在马铃薯对渗透、干旱和高盐等胁迫响应中起正向调控作用。本研究也为StFBH3基因在马铃薯中的生物学功能深入理解提供一定的参考依据。

关键词：马铃薯 StFBH3 转录因子逆境胁迫

维普期刊数据库博看期刊

同方期刊数据库

New Exact Solutions for Konopelchenko-Dubrovsky Equation Using an Extended Riccati Equation Rational Expansion Method

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2006年第5期45卷 I0003-I0003,770-776页

作者： song Li-na ZHANG Hong-Qing Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

Taking the Konopelchenko-Dubrovsky system as a simple example, some familles of rational formal hyperbolic function solutions, rational formal triangular periodic solutions, and rational solutions are constructed by using the extended Riccati equation rational expansion method presented by us. The method can also be applied to solve more nonlinear partial differential equation or equations.

关键词： Konopelchenko-Dubrovsky equation extended Riccati equation rational expansion method nonlinear partial differential equation or equations

Abundance and Community Composition of Ammonia-Oxidizers in Paddy Soil at Different Nitrogen Fertilizer Rates

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第4期13卷 870-880页

作者： song Ya-na LIN Zhi-min Institute of Biological Technology Fujian Academy of Agricultural Sciences

Ammonia oxidation, the first and rate-limiting step of nitrification, is carried out by both ammonia-oxidizing bacteria （AOB） and ammonia-oxidizing archaea （AOA）. However, the relative importance of AOB and AOA to nitrification in terrestrial ecosystems is not well understood. The aim of this study was to investigate the effect of the nitrogen input amount on abundance and community composition of AOB and AOA in red paddy soil. Soil samples of 10-20 cm （root layer soil） and 0-5 cm （surface soil） depths were taken from a red paddy. Rice in the paddy was fertilized with different rates of N as urea of N1 （75 kg N ha＂ yr-1）, N2 （150 kg N ha~ yrl）, N3 （225 kg N ha1 yrl） and CK （without fertilizers） in 2009, 2010 and 2011. Abundance and community composition of ammonia oxidizers was analyzed by real-time PCR and denaturing gradient gel electrophoresis （DGGE） based on amoA （the unit A of ammonia monooxygenase） gene. Archaeal amoA copies in N3 and N2 were significantly （P〈0.05） higher than those in CK and N1 in root layer soil or in surface soil under tillering and heading stages of rice, while the enhancement in bacterial amoA gene copies with increasing of N fertilizer rates only took on in root layer soil. N availability and soil NO3--N content increased but soil NH4＋-N content didn＇t change with increasing of N fertilizer rates. Otherwise, the copy numbers of archaeal amoA gene were higher （P〈0.05） than those of bacterial amoA gene in root lary soil or in surface soil. Redundancy discriminate analysis based on DGGE bands showed that there were no obvious differs in composition of AOA or AOB communities in the field among different N fertilizer rates. Results of this study suggested that the abundance of ammonia-oxidizers had active response to N fertilizer rates and the response of AOA was more obvious than that of AOB. Similarity in the community composition of AOA or AOB among different N fertilizer rates indicate that the community composition of ammonia-oxidizers w

关键词： ammonia-oxidizing bacteria ammonia-oxidizing archaea nitrogen fertilizer rates paddy soil

A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata

在线全文

学校读者我要写书评

暂无评论

Plant Diversity 2019年第1期41卷 7-12页

作者： Junbin Cheng na song Jinsong Wu Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences

Although patatin was initially identified as a major storage protein in potato tubers, patatin-like proteins(PLPs) have been recently reported to be widely present in many plant species and shown to be involved in plant-pathogen interactions. However, it is not clear whether PLPs are involved in Nicotiana attenuata resistance against the necrotrophic fungal pathogen, Alternaria alternata. In this study we identified a naPLP gene, whose expression was highly elicited by A. alternata inoculation. Silencing naPLP enhanced N. attenuata resistance to A. alternata, which was associated with higher induction levels of JA and ethylene biosynthetic genes, naACS1, naACO1 and naLOX3. The induction of naPLP expression by the fungus was abolished in JA-deficient plants and significantly reduced in ethylene-insensitive plants. In addition, naPLP transcripts were highly induced by exogenous treatment with either methyl jasmonate(MeJA) or ethephon. Co-treatment with MeJA and ethephon led to a much higher induction level of naPLP transcripts, and this synergistic induction was largely dependent on endogenous JA and ethylene signaling pathways. Thus, we conclude that the naPLP gene is elicited by A. alternata via JA and ethylene signaling pathways in a synergistic way; however, unlike other JA-and ethylene-induced defense genes,naPLP negatively affects plant resistance to the fungus likely by suppressing JA and ethylene biosynthetic gene expression.

关键词： Alternaria Ethylene Jasmonate Necrotrophic fungal pathogen Nicotiana Plant resistance

Extended sine-Gordon Equation Method and Its Application to Maccari＇s System

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2005年第5X期44卷 783-788页

作者： song Li-na ZHANG Hong-Qing Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

An extended sine-Gordon equation method is proposed to construct exact travelling wave solutions to Maccari's equation based upon a generalized sine-Gordon equation. It is shown that more new travelling wave solutions can be found by this new method, which include bell-shaped soliton solutions, kink-shaped soliton solutions, periodic wave solution, and new travelling waves.

关键词： Maccari's system exact travelling wave solution extended sine-Gordon equation method

Extended Jacobi Elliptic Function Rational Expansion Method and Its Application to （2＋1）-Dimensional Stochastic Dispersive Long Wave System

在线全文

学校读者我要写书评

暂无评论

Communications in Theoretical Physics 2007年第6期47卷 969-974页

作者： song Li-na ZHANG Hong-Qing Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

In this work, by means of a generalized method and symbolic computation, we extend the Jacobi elliptic function rational expansion method to uniformly construct a series of stochastic wave solutions for stochastic evolution equations. To illustrate the effectiveness of our method, we take the （2＋ 1）-dimensional stochastic dispersive long wave system as an example. We not only have obtained some known solutions, but also have constructed some new rational formal stochastic Jacobi elliptic function solutions.

关键词： stochastic evolution equations (2+ 1)-dimensional stochastic dispersive long wave system rational formal stochastic Jacobi elliptic function solutions

Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Oceanology and Limnology 2017年第1期35卷 198-202页

作者： song na Fisheries College Ocean University of China Qingdao 266000 China Fisheries College Zhejiang Ocean University Zhoushan 316000 China national Research Institute of Fisheries Science Fisheries Research Agency Yokohama Japan

Sebastiscus marmoratus is an important sedentary ovoviparous fish distributed in near-shore coastal waters from the coast of China to Japan. Candidate S. marmoratus microsatellite markers were developed in the present study using 454 pyrosequencing, and the marker profile was analyzed. A total of 2 000 000 raw sequence reads were assembled to reduce redundancy. Among them, 1 043 dinucleotide, 925 trinucleotide, 692 tetranucleotide, and 315 pentanucleotide repeats were detected. AC repeats were the most frequent motifs among the dinucleotide repeats, and AAT was the most abundant among the trinucleotide repeats. AAAT, ATAG, and ATCC were the three most common tetranucleotide motifs, and AAGAT and AATAT were the most dominant pentanucleotide motifs. The greatest numbers of loci and potentially amplifiable loci were found in dinucleotide repeats, whereas trinucleotide repeats had the fewest. In summary, a wide range of candidate microsatellite markers were identified in the present study using a rapid and efficient 454 pyrosequencing approach.

关键词： marbled rockfish microsatellite 454 FLX pyrosequencing genomic Dna

The mitochondrial genome of Chaeturichthys stigmatias provides novel insight into the interspecific difference with Amblychaeturichthys hexanema

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2021年第9期40卷 74-81页

作者： Jian Zheng Bingjie Chen Tianxiang Gao na song Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China

Chaeturichthys stigmatias and Amblychaeturichthys hexanema belong to the family Gobiidae,which are offshore warm fish species and widely distribute in the western Pacific *** this study,the mitochondrial cytochrome c oxidase subunit I(COI)sequences and 12 S ribosomal Rna(12 S rRna)sequences were used to analyze the interspecific differences between the two *** phylogenetic analysis showed that the interspecific distance was significantly higher than the intraspecific genetic *** Neighbor-Joining tree showed two separate clusters,without sharing *** mitochondrial genome sequence of *** was also *** genome was 17134 bp in size,with a high A+T content of 55.9%.The phylogenetic analysis based on the tandem 13 coding protein genes nucleotide sequences indicated that *** showed a close relationship with *** study can provide the basic genetic data for two species and will help for constructing the phylogeny of the Gobiiade.

关键词： Chaeturichthys stigmatias Amblychaeturichthys hexanema mitochondrial genome phylogenetic analysis

Genetic variation of the small yellow croaker(Larimichthys polyactis)inferred from mitochondrial Dna provides novel insight into the fluctuation of resources

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2022年第11期41卷 88-95页

作者： Jian Zheng Tianxiang Gao Yunrong Yan na song Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China

The small yellow croaker(Larimichthys polyactis)belongs to the family Sciaenidae,which is an offshore warm fish species and widely distributed in the western *** this study,the variation of genetic diversity and genetic differentiation among *** populations was analyzed by mitochondrial Dna control region.A total of 110 polymorphic sites were checked,which defined 134 *** level of haplotype diversity(h=0.993±0.002)was detected in the examined *** genetic structure analyse(analysis of molecular variance,Fst)showed there were high gene flow among *** *** result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations,which can be attributed to diverse habitats,wide distribution range and high mutation rate of control *** phylogenetic methods,coalescent analyses(neutrality tests,mismatch distribution analysis,Bayesian skyline analyses)and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence,we inferred that the genetic make-up of extant populations of *** was shaped by Pleistocene environmental impacts on the historical demography of this ***,relatively constant genetic diversity and larger effective population size were detected in recent *** *** result showed that the fishing policy certainly,such as the summer closed fishing,played a role in protecting resources of *** study can offer a wealth of biological novelties which indicates genetic structure of *** population and provides the foundation for resources protection and policy setting.

关键词： Larimichthys polyactis genetic diversity genetic differentiation mitochondrial Dna control region