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Comparative transcriptome analysis provides insights into the mechanism of pear dwarfing

Journal of Integrative Agriculture 2022年第7期21卷 1952-1967页

作者： TANG Zi-kai SUN Man-yi LI Jia-ming song bo-bo LIU Yue-yuan TIAN Yi-ke WANG Cai-hong WU Jun College of Horticulture State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjing 210095P.R.China College of Horticulture Qingdao Agricultural UniversityQingdao 266109P.R.China

Dwarfism is an important trait which is closely related to the efficiency of fruit orchard management and ***,dwarfing cannot be widely applied in the cultivation of pears,especially Asian *** varieties with dwarf characteristics is a goal of paramount importance in pear *** the present study,dwarf phenotype pears(DPPs)and arborescent phenotype pears(APPs)were obtained from the offspring of a cross between‘Aiyuxiang’and‘Cuiguan’pear cultivars,which exhibited dwarfed and arborescent statures,*** compared with APPs,the heights of DPPs showed a 62.8%reduction,and the internode lengths were significantly *** between DPPs and APPs demonstrated that the dwarfed phenotype of DPPs was primarily induced by the aerial portions of the plant,and independent of the root *** of stem tissue sections showed that DPP cells were arranged chaotically with irregular shapes,and the average length was larger than that of the APP cells.A total of 1401 differently expressed genes(DEGs)in shoot apices between DPPs and APPs were identified by RNA-sequencing(RNA-Seq),and these DEGs were mainly enriched in the‘phytohormone-related pathways,cell wall metabolism and cell division’***,101 DEGs were identified as transcription factors(TFs).In DPPs,several brassinosteroids(BR)signaling and cell cycle-related genes were significantly down-regulated,while genes involved in BR and GA degradation were *** analysis of RNA-Seq data and stem tissue sections suggested that the dwarfed phenotype of DPPs could be primarily attributed to deficiencies in cell *** work using simple sequence repeat(SSR)markers narrowed the location of the gene responsible for the dwarf phenotype of‘Le Nain Vert’.Through combined analysis of our transcriptomic data with the SSR results,we identified four genes as promising candidates for the dwarf phenotype,among which,a DELLA gene could be the

关键词： pear dwarf phenotype RNA-Seq cell division phytohormones

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Competitive binding of postsynaptic density 95 and Ca2+-calmodulin dependent protein kinase II to N-methyl-D-aspartate receptor subunit 2B in rat brain

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Acta Pharmacologica Sinica 2004年第2期25卷 50-54页

作者： Fan-jie MENG , Jun GUO, bo song, Xue-bo YAN, Guang-yi ZHANG2 Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China Research Center of Biochemistry and Molecular Biology Xuzhou Medical College Xuzhou 221002

AIM: To investigate the interactions among postsynaptic density 95 (PSD-95), Ca2+-calmodulin dependent protein kinase IIα (CaMKIIα), and N-methyl-D-aspartate receptor subunit 2B (NR2B) during ischemia and reperfusion in hippocampus of rats. METHODS: Brain ischemia was induced by four-vessel occlusion procedure in rats. Immu- noprecipitation and immunoblotting were performed to study the interactions and phosphorylation of proteins. The association-dissociation of PSD-95 and CaMKIIα to and from N-methyl-D-aspartate (NMDA) receptor induced by ischemia and reperfusion and the effects of 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenyl-pip- erazine (KN-62, a selective inhibitor of CaMKII) on these protein interactions were investigated. Coimmuno- precipitation and immunoblotting were performed for the studies of interactions among proteins. RESULTS: The alternations of the binding level of PSD-95 and CaMKIIα to NR2B during ischemia and reperfusion demonstrated the negative correlation to each other. Pre-administration of KN62 through both cerebral ventricles inhibited the 10 min ischemia-induced increase of the binding of PSD-95 to NR2B and, on the contrary, promoted the binding of CaMKIIα to NR2B. CONCLUSION: PSD-95 competes with CaMKII to bind to NR2B during ischemia and reperfusion in rat hippocampus.

关键词： N-methyl-D-aspartate receptors Ca2+-calmodulin dependent protein kinase brain ischemia

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boosting charge extraction and efficiency of inverted perovskite solar cells through coordinating group modification at the buffer layer/cathode interface

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Nano Research 2025年第1期18卷 415-423页

作者： Zhiqing Liang Ziqiu Ren Ziyu Wang Nan Yan Ling Li bo Zhang Yanlin song College of Chemistry Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education Shaanxi Key Laboratory for Advanced Energy DevicesShaanxi Engineering Lab for Advanced Energy Technology School of Materials Science and EngineeringShaanxi Normal UniversityXi’an 710119China Key Laboratory of Green Printing Institute of ChemistryChinese Academy of Sciences(ICCAS)Beijing Engineering Research Center of Nanomaterials for Green Printing TechnologyNational Laboratory for Molecular Sciences(BNLMS)Beijing 100190China

In inverted perovskite solar cells (PSCs),effective modification of the interface between the metalcathode and electron transport layer (ETL) is crucial forachieving high performance and stability. Herein, sulfonatedbathocuproine, commonly known as disodium bathocuproinedisulfonate (BCDS), was employed as a cathode buffer layerto address the interfacial issues at the [6,6]-phenyl-C61-butyricacid methyl ester (PCBM)/Ag interface. BCDS possesses theability to form coordinate bonds with Ag electrodes. Theutilization of the BCDS buffer layer enhanced the chargeextraction capability at the cathode interface whilesimultaneously achieving interfacial defect passivation,improving interfacial contact and increasing the built-in electricfield. Consequently, a power conversion efficiency (PCE) of25.06% was achieved. Furthermore, owing to the excellent filmforminguniformity of BCDS on PCBM, the stability of thedevice was also improved. After storage in dry air for morethan 2000 h, the device maintained 96% of its initial efficiency. This work underscores the remarkable potential of tailoringcoordination groups to enhance charge extraction efficiency at the ETL-cathode interface, unveiling a promising newfrontier in buffer layer development and performance optimization strategies for PSCs.

关键词： perovskite solar cells buffer layer bathocuproine disulfonate coordination group modification electron transport layer(ETL)-cathode interface

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粒径对煤低温氧化阶段表观活化能影响试验研究

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煤炭工程 2020年第10期52卷 143-148页

作者：翟小伟成倬徐启飞余林尚博宋波波西安科技大学安全科学与工程学院陕西西安710054 西部矿井开采及灾害防治教育部重点实验室陕西西安710054 贵州黔西能源开发有限公司青龙煤矿贵州毕节551507

为探究不同粒径对煤自燃表观活化能的影响,对青龙煤矿三个煤层的不同粒径煤样进行程序升温试验,分析其耗氧速率、CO和C2H4产生规律,得到不同煤样的临界温度点和干裂温度点,并据此将煤低温氧化过程分为3个阶段。通过建立的基于耗氧速率... 详细信息

为探究不同粒径对煤自燃表观活化能的影响,对青龙煤矿三个煤层的不同粒径煤样进行程序升温试验,分析其耗氧速率、CO和C2H4产生规律,得到不同煤样的临界温度点和干裂温度点,并据此将煤低温氧化过程分为3个阶段。通过建立的基于耗氧速率的阿伦尼乌斯公式对试验数据进行处理,得出各煤样不同阶段的表观活化能和指前因子。结果表明:处于相同变化阶段的煤样表观活化能随粒径的增大而增大;对相同粒径煤样而言,若S1阶段的指前因子变化较小,其表观活化能随反应的进行而增大,若S1阶段的指前因子变化较大,则其S1阶段表观活化能大于S2阶段。5~7mm粒径作为临界粒径,其S1、S2阶段表观活化能相较大于其他粒径。混合粒径煤样各阶段的表观活化能较小。对比各组煤样的表观活化能可知,在相同的情况下,18#煤层发生自燃的难度相对较大,16#煤层次之,17#煤层难度相对较小。

关键词：煤自燃低温氧化表观活化能指前因子程序升温

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啤酒厂污泥与鸡粪接种菌剂堆肥除臭效果及其作用机制

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农业环境科学学报 2011年第6期30卷 1236-1241页

作者：潘飞蔡映杰俞武宋博博曾庆福武汉纺织大学纺织印染清洁生产教育部工程研究中心武汉430073

通过探讨功能性菌剂对堆肥化进程的影响,为减少啤酒厂污泥与鸡粪堆肥臭气产生提供理论依据,将自制具有去除臭味能力的微生物菌剂DF-1接种至啤酒厂污泥与鸡粪的堆体中,测定堆肥化过程中各参数的变化和微生物群落及菌剂DF-1中优势微生物... 详细信息

通过探讨功能性菌剂对堆肥化进程的影响,为减少啤酒厂污泥与鸡粪堆肥臭气产生提供理论依据,将自制具有去除臭味能力的微生物菌剂DF-1接种至啤酒厂污泥与鸡粪的堆体中,测定堆肥化过程中各参数的变化和微生物群落及菌剂DF-1中优势微生物变化情况,以不接种堆体为参照。结果显示,菌剂DF-1中优势菌种为乳酸乳球菌、热带假丝酵母及绿色木霉。相对于不接种菌剂的堆体而言,接种菌剂DF-1的啤酒厂污泥与鸡粪堆体在升温期和高温期仅感觉到微弱的臭味,降温期勉强感觉到臭味,堆肥达到稳定状态后则感觉不到臭味。堆肥的升温期,菌剂中优势菌种乳酸乳球菌,热带假丝酵母和绿色木霉均可检测到;堆肥的高温期,菌剂中优势菌种仅检测到乳酸乳球菌;堆肥的降温期和腐熟期,菌剂中优势菌种仅检测到绿色木霉;菌剂DF-1中优势微生物在堆肥过程中存在一个交替演变的过程。

关键词：堆肥啤酒厂污泥鸡粪除臭微生物群落

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基于官能团演化特性的煤氧化动力学研究

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西安科技大学学报 2020年第1期40卷 71-77页

作者：马腾陈晓坤翟小伟白亚娥雷昌奎宋波波西安科技大学安全科学与工程学院陕西西安710054 西安科技大学陕西省煤火灾害防治重点实验室陕西西安710054 神木职业技术学院矿业工程系陕西神木719300

为了深入研究煤自燃微观结构的动力学特征,利用同步热分析和原位傅里叶变换红外光谱技术测试顾北烟煤氧化过程中重量与官能团随温度的变化规律。通过Achar微分法与Coast-Redfern积分法相结合对官能团进行动力学计算,并采用Bagchi法推断... 详细信息

为了深入研究煤自燃微观结构的动力学特征,利用同步热分析和原位傅里叶变换红外光谱技术测试顾北烟煤氧化过程中重量与官能团随温度的变化规律。通过Achar微分法与Coast-Redfern积分法相结合对官能团进行动力学计算,并采用Bagchi法推断了最概然机理函数。结果表明:煤氧升温过程呈阶段性变化规律,分为低温失重阶段、吸氧增重阶段、慢化学反应阶段、燃烧阶段和燃尽阶段。煤结构中主要官能团—OH,C O,—CH 2,C—H与C C键在各阶段体现出不同氧化特性,在低温失重阶段—OH,—CH 2与C—H键参与反应所需要的活化能与遵循动力学模型不同,而C O与C C键结构稳定不易参与反应,从吸氧增重阶段至燃烧阶段,官能团逐渐被激活与氧气发生反应,燃尽阶段官能团基本耗尽。官能团动力学计算结果揭示了煤自燃是各类官能团遵循不同的动力学模型逐步活化的过程。

关键词：安全科学与工程煤自燃动力学官能团阶段性

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A pre-strain strategy of current collectors for suppressing electrode debonding in lithium-ion batteries

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Applied Mathematics and Mechanics(English Edition) 2023年第4期44卷 547-560页

作者： bo RUI bo LU Yicheng song Junqian ZHANG Department of Mechanics Shanghai Institute of Applied Mathematics and MechanicsSchool of Mechanics and Engineering ScienceShanghai UniversityShanghai 200444China Shanghai Key Laboratory of Mechanics in Energy Engineering Shanghai UniversityShanghai 200444China Shanghai Frontier Science Center of Mechanoinformatics Shanghai UniversityShanghai 200444China Zhejiang Laboratory Hangzhou 311100China

The interfacial debonding between the active layer and the current collector has been recognized as a critical mechanism for battery fading,and thus has attracted great efforts focused on the related ***,much still remains to be studied regarding practical methods for suppressing electrode debonding,especially from the perspective of *** this paper,a pre-strain strategy of current collectors to alleviate electrode debonding is *** analytical model for a symmetric electrode with a deformable and limited-thickness current collector is developed to analyze the debonding behavior involving both a pre-strain of the current collector and an eigen-strain of the active *** results reveal that the well-designed pre-strain can significantly delay the debonding onset(by up to 100%)and considerably reduce the debonding *** critical values of the pre-strain are identified,and the pre-strain design principles are also *** on these findings,this work sheds light on the mechanical design to suppress electrode degradation.

关键词： pre-strain current collector electrode debonding lithium-ion battery(LIB) mechanically-based design

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Analysis of the anti-T2DM components in dichloromethane fraction of Schisandra sphenanthera and its mechanism

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Traditional Medicine Research 2025年第1期10卷 27-43页

作者： Shi-bo Feng Hai-Hui Jiang Hui-Li Kang Yi Jiang Hua-Wei Zhang Xiao-Mei song Yu-Ze Li Wen-Li Huang Dong-Dong Zhang Chong Deng School of Pharmacy Shaanxi University of Chinese MedicineXianyang 712046China School of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine Shaanxi University of Chinese MedicineXianyang 712046China Key Laboratory for Research and Development of“Qin Medicine”of Shaanxi Administration of Traditional Chinese Medicine Xianyang 712046China Shaanxi Key Laboratory of Research and Application of“Taibai Qi Yao” Xianyang 712046China

Background:The type 2 diabetes mellitus(T2DM)pharmacodynamic study of various parts of Schisandra sphenanthera was conducted in the previous stage,and it was found that dichloromethane extracted part(SDP)had a significant hypoglycemic ***,the components of SDP were analyzed,and the specific mechanism of its anti-T2DM was ***:We used a high-fat,high-sugar diet in combination with streptozotocin to induce a T2DM rat model,and the model rats were divided into two groups according to body weight and blood ***,oral glucose tolerance test,fasting blood glucose,low density lipoprotein cholesterol,superoxide dismutase,insulin,glycated hemoglobin,total cholesterol,nonesterified free fatty acids,alanine aminotransferase,high-density lipoprotein cholesterol,aspartate aminotransferase,malondialdehyde,and glutathione peroxidase were measured,organ indices were calculated,and pathological sections of pancreas and liver were *** 16S rRNA V3–V4 region of intestinal flora was sequenced to explore the effect of SDP on biochemical indicators and intestinal *** on the above indicators,the anti-T2DM mechanism of SDP in Schisandra sphenanthera was ***:After six weeks of administration,the biochemical indices of diabetic rats were diminished compared to the control *** SDP could significantly increase the gut microbialα-diversity index,resulting in significant changes in the flora of T2DM rats,with increased richness and diversity,reduced harmful flora,and significantly back-regulated the levels of acetic acid,propionic acid,and butyric ***:SDP can improve the symptoms associated with elevated blood glucose,dyslipidemia,elevated fasting insulin levels,and damaged glucose tolerance in *** against T2DM may be through the control of intestinal flora to normalize and exert anti-diabetic effect;its main active components may be lignans and terpenoids.

关键词： Schisandra sphenanthera T2DM intestinal flora HPLC

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Quantum essence of particle superfluidity

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Nano Research 2022年第6期15卷 5230-5234页

作者： bo song Lei Jiang School of Optical-Electrical Computer Engineering University of Shanghai for Science and TechnologyShanghai 200093China Key Laboratory of Bio-inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China

Life systems show an ultralow energy consumption in their high-efficiency bio-activities,implying a high-flux transport of ions and molecules with an ultralow resistivity.A collective motion(CM)of these particles is necessary for this kind of behaviors,different from the traditional Newtonian *** CM is an ordered particle state,resulting from the balance between attraction and repulsion of the particles,in which the attraction is a necessary *** ultralow resistivity of electronic or atomic fluid at low temperature is already described phenomenologically by introducing the interparticle ***,we try to establish a phenomenological expression for the quantum state of ion or molecule CM at ambient temperature,by also considering the attraction of *** studies suggest that the bose-Einstein condensate potentially exists widely.

关键词： ultralow resistivity flow collective motion ions and molecules Ginzburg-Landau equation

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A four-dimensional model for the information storage/output of life

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Nano Research 2023年第2期16卷 2630-2634页

作者： bo song Lei Jiang Key Laboratory of Optical Technology and Instrument for Medicine Ministry of EducationShanghai Key Lab of Modern Optical SystemSchool of Optical-Electrical Computer EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China Key Laboratory of Bio-inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China

A large amount of progress has achieved in neuroscience,however,there is still a lack of reasonable model for the storage/output(S/O)of life *** cyclical motion of cardio-and pulmonary-myocyte is a typical process of the life information S/O,while the opening and closing sites of Ca^(2+)ion channels during the motion can form a genetically programmed time-dependent three-dimensional(3D)*** phenomena indicate a strong correlation of the information S/O model of these myocytes with the time-sequence 3D ***,based on the time-dependent Ca^(2+)fluorescence imaging during the motion of cardio-and pulmonary-myocyte,here we suggest a four-dimensional(4D)code of information S/O model in cell and nervous *** from the fact of pulmonary myocyte motion able to be controlled by brain,it is deduced that the 4D code in brain has a role of controlling muscles through a pathway of the central nervous system,peripheral nervous system,neuromuscular junction,and muscle *** addition,we also suggested the 4D code of non-innate skill that can be programmed by the learning/training of a long time(~3 years),such as walking,writing,painting,sports,speech,singing,and ***,this 4D S/O model is reasonable for the ultralow energy consumption of life information transmission.

关键词： four-dimensional(4D)storage/output model life information cell nervous system

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