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Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity

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Journal of Integrative Agriculture 2015年第8期14卷 1627-1636页

作者： song bing-yan SHI Jin-xiao song song-quan College of Biology Resources and Environmental Sciences Jishou University Institute of Botany Chinese Academy of Sciences

Echinochloa crus-galli, one of the world＇s most serious weeds, influences seriously the yield and quality of cereal crop *** is well known that ***-galli grain is dormant, but its dormant type, as well as its dormancy release and germination in relation to galactomannan-hydrolysing enzyme activity were poorly *** cooperation of endo-β-mannanase（EC 3.***.1.78）, β-mannosidase（EC 3.***.1.25） and α-galactosidase（EC 3.***.1.22） can hydrolyze the cell walls rich in mannan-based *** the present paper, the mature grains are used as experimental materials, we investigated the water uptake of grains, the effect of removing partial endosperm, after-ripening, stratification and phytohormone on grain germination, and the change in endo-β-mannanase, β-mannosidase and α-galactosidase activities of grains during after-ripening and *** results showed that the freshly-collected grains were water-permeable and had only phase I and II of water uptake, while the grains after-ripened for 150 d had an obvious phase III of water *** alternating photoperiod, the germination of grains freshly-collected was zero at 10–35°C, and that of half grains was 11% at 20°C *** grain germination was notably promoted by after-ripening and stratification, but not by gibberellic acid and 6-benzyladenine at 0.0001–1 mmol L–1.β-Mannosidase activity increased during 0 to 300 d of after-ripening and then *** activity of endo-β-mannanase and α-galactosidase of grains decreased with *** grain germination, endo-β-mannanase and β-mannosidase activities obviously increased, while α-galactosidase activity *** data showed that ***-galli grain was a deep physiological dormant, the dormancy release by after-ripening was related to an increasing β-mannosidase activity, and its germination was closely associated with an increasing endo-β-mannanase and β-mannosidase activity; which have provided new knowledge to decrease the harm of ***

关键词： after-ripening deep physiological dormancy Echinochloa crus-galli galactomannan-hydrolysing enzyme phytohormone stratification

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蜡状芽孢杆菌CZ磷酸甘露糖异构酶基因的克隆表达及酶学性质研究

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食品工业科技 2017年第6期38卷 195-200页

作者：张瑶崔堂兵宋妍华南理工大学生物科学与工程学院广东广州510006

将蜡状芽孢杆菌CZ中的磷酸甘露糖异构酶基因(pmi)进行克隆,并在大肠杆菌中进行异源表达。将PCR扩增得到的pmi基因与p ET-22b(+)表达载体进行连接,转入大肠杆菌BL21(DE3)中,构建重组菌株BL21-p ET22b(+)-pmi,并成功表达了重组磷酸甘露糖... 详细信息

将蜡状芽孢杆菌CZ中的磷酸甘露糖异构酶基因(pmi)进行克隆,并在大肠杆菌中进行异源表达。将PCR扩增得到的pmi基因与p ET-22b(+)表达载体进行连接,转入大肠杆菌BL21(DE3)中,构建重组菌株BL21-p ET22b(+)-pmi,并成功表达了重组磷酸甘露糖异构酶。结果显示:克隆得到pmi基因序列全长为948 bp,编码315个氨基酸。通过镍柱His Trap HP亲和层析法纯化得到具有活性的重组酶,其蛋白分子大小约为40.8 ku。酶学性质结果显示:该酶的最适反应温度为35℃,在30~40℃酶活力相对稳定;最适p H为7.0,在弱碱性条件下保存12 h后仍存有50%以上酶活力;不同低浓度的金属离子Ni^(2+)、Ca^(2+)、Zn^(2+)、Cu^(2+)和Mg^(2+)均对该酶表现出不同程度的激活作用,其中Mn^(2+)对该酶激活作用最显著,当其浓度为1 mmol/L时,激活作用最大,而Co^(2+)对其有明显的抑制作用。

关键词：磷酸甘露糖异构酶(PMI) 蜡状芽孢杆菌克隆表达酶学性质

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Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons

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Neural Regeneration Research 2025年第6期20卷 1816-1824页

作者： Jiantao Cui Yueru Shen Zheng song Dinggang Fan bing Hu Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina

Rab5 is a GTPase protein that is involved in intracellular membrane trafficking. It functions by binding to various effector proteins and regulating cellular responses, including the formation of transport vesicles and their fusion with the cellular membrane. Rab5 has been reported to play an important role in the development of the zebrafish embryo;however, its role in axonal regeneration in the central nervous system remains unclear. In this study, we established a zebrafish Mauthner cell model of axonal injury using single-cell electroporation and two-photon axotomy techniques. We found that overexpression of Rab5 in single Mauthner cells promoted marked axonal regeneration and increased the number of intra-axonal transport vesicles. In contrast, treatment of zebrafish larvae with the Rab kinase inhibitor CID-1067700markedly inhibited axonal regeneration in Mauthner cells. We also found that Rab5 activated phosphatidylinositol 3-kinase(PI3K) during axonal repair of Mauthner cells and promoted the recovery of zebrafish locomotor function. Additionally, rapamycin, an inhibitor of the mechanistic target of rapamycin downstream of PI3K, markedly hindered axonal regeneration. These findings suggest that Rab5 promotes the axonal regeneration of injured zebrafish Mauthner cells by activating the PI3K signaling pathway.

关键词： axonal regeneration Mauthner cell nerve regeneration Rab5 zebrafish

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“四个自信”视域下会计学原理课程思政的实践探索

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新疆职业教育研究 2023年第1期14卷 19-25页

作者：宋艳冰辽宁省交通高等专科学校马克思主义学院辽宁沈阳110122

分析“四个自信”视域下会计学原理课程思政的现状,创新课程思政建设,通过构建“1+N”模式、探索课程思政融合路径、优化教学设计和搭建全媒体矩阵方式,寻找思政教育与专业课教学结合的新契机,推动“四个自信”融入会计学原理课程教学中... 详细信息

分析“四个自信”视域下会计学原理课程思政的现状,创新课程思政建设,通过构建“1+N”模式、探索课程思政融合路径、优化教学设计和搭建全媒体矩阵方式,寻找思政教育与专业课教学结合的新契机,推动“四个自信”融入会计学原理课程教学中,强化当代大学生对“四个自信”的价值认同,为社会主义建设培养综合性会计人才。

关键词：四个自信会计学原理课程思政思想政治教育

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清水江木商账簿中的记账符号考释

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原生态民族文化学刊 2014年第3期6卷 31-36页

作者：宋冰雁贵州大学法学院贵州贵阳550000

账簿是对生产经营的收支记录,通过对其中记载内容的分析提炼,可以构建出历史时期各种市场的经营状态。理清清水江账簿文书中较难理解的各种记账特殊符号,能为以后进一步研究清水江账簿文书提供帮助。

关键词：清水江文书账簿记账符号

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Neuronal regulated cell death in aging-related neurodegenerative diseases:key pathways and therapeutic potentials

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Neural Regeneration Research 2025年第8期20卷 2245-2263页

作者： Run song Shiyi Yin Jiannan Wu Junqiang yan Department of Neurology The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina Neuromolecular Biology Laboratory The First Affiliated HospitalCollege of Clinical Medicine of Henan University of Science and TechnologyLuoyangHenan ProvinceChina

Regulated cell death(such as apoptosis,necroptosis,pyroptosis,autophagy,cuproptosis,ferroptosis,disulfidptosis)involves complex signaling pathways and molecular effectors,and has been proven to be an important regulatory mechanism for regulating neuronal aging and ***,excessive activation of regulated cell death may lead to the progression of aging-related *** review summarizes recent advances in the understanding of seven forms of regulated cell death in age-related ***,the newly identified ferroptosis and cuproptosis have been implicated in the risk of cognitive impairment and neurodegenerative *** forms of cell death exacerbate disease progression by promoting inflammation,oxidative stress,and pathological protein *** review also provides an overview of key signaling pathways and crosstalk mechanisms among these regulated cell death forms,with a focus on ferroptosis,cuproptosis,and *** instance,FDX1 directly induces cuproptosis by regulating copper ion valency and dihydrolipoamide S-acetyltransferase aggregation,while copper mediates glutathione peroxidase 4 degradation,enhancing ferroptosis ***,inhibiting the Xc-transport system to prevent ferroptosis can increase disulfide formation and shift the NADP^(+)/NADPH ratio,transitioning ferroptosis to *** insights help to uncover the potential connections among these novel regulated cell death forms and differentiate them from traditional regulated cell death *** conclusion,identifying key targets and their crosstalk points among various regulated cell death pathways may aid in developing specific biomarkers to reverse the aging clock and treat age-related neurodegenerative conditions.

关键词： apoptosis autophagy cuproptosis disulfidptosis ferroptosis necroptosis neurodegenerative disease neurological aging diseases PANoptosis pyroptosis

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怀玉山产三叶青叶绿体基因组特征及其系统进化关系

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中草药 2023年第16期54卷 5358-5371页

作者：洪森荣刘雯莉宋冰雁颜玉情上饶师范学院生命科学学院江西上饶334001 上饶农业技术创新研究院江西上饶334001 上饶市药食同源植物资源保护与利用重点实验室江西上饶334001 上饶市三叶青保育与利用技术创新中心江西上饶334001

目的解析怀玉山产三叶青Tetrastigmahemsleyanum叶绿体基因组信息序列特征和确定其在崖爬藤属的系统位置。方法用Illumina高通量测序平台Nova Seq6000进行测序获得怀玉山产三叶青叶绿体基因组序列,借助Ge Seq、t RNAscan-SE、MISA、VIST... 详细信息

目的解析怀玉山产三叶青Tetrastigmahemsleyanum叶绿体基因组信息序列特征和确定其在崖爬藤属的系统位置。方法用Illumina高通量测序平台Nova Seq6000进行测序获得怀玉山产三叶青叶绿体基因组序列,借助Ge Seq、t RNAscan-SE、MISA、VISTA tools、DNADna SP6.0、JSHYCloud、CodonW1.4.2、Pasteur Galaxy、mafft 7.0、fasttree 2.1.10等生物信息学工具进行序列分析、密码子偏好分析、崖爬藤属基因组比较分析和系统发育研究。结果怀玉山产三叶青Tetrastigma hemsleyanum叶绿体基因组为共价闭合双链环状分子,长160165 bp,包含1个大单拷贝区(large single copy region,LSC)、1个反向重复区a(inverted repeat region a,IRa)、1个反向重复区b(inverted repeat region b,IRb)和1个小单拷贝区(small single copy region,SSC);怀玉山产三叶青叶绿体基因组注释到光合作用基因、自我复制基因、其他基因和未知功能基因4类共133个基因,包括88个CDS基因、37个t RNA基因和8个r RNA基因;怀玉山产三叶青叶绿体基因组检测到63个SSR位点,其中单碱基重复56个,双碱基重复7个;怀玉山产三叶青叶绿体基因组核苷酸多样性的变化范围为0~0.23418,基因间隔区trn S-GCU-trn G-UCC、rps16-trnG-UCC以及基因ycf1变异率最高。怀玉山产三叶青叶绿体基因组密码子偏好以A或U结尾,具有较强的偏好性。突变对密码子偏好性的影响较强,而其他因素如自然选择对密码子的偏好性影响较小。怀玉山产三叶青叶绿体基因组有31个高频密码子,16个以U结尾,13个以A结尾,2个以G结尾,其中13个为最优密码子。怀玉山产三叶青与福建产三叶青(MW375708)和广西产三叶青(MW375709)亲缘关系较近,与浙江产三叶青(MT827073)、浙江产三叶青(MW375707)、浙江产三叶青KT033563(NC_029339)、四川产三叶青(MW375710)和浙江产三叶青(MW375711)亲缘关系较远。结论三叶青之间的亲缘关系与地理位置相关性不大。首次采用生物信息学分析方法对怀玉山产三叶青叶绿体基因组进行了全面、深度解析,结果将为崖爬藤属药用植物的遗传多样性分析以及新品系的遗传育种研究等提供理论依据。

关键词：三叶青叶绿体基因组特征系统进化关系大单拷贝区小单拷贝区

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Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang bing Zhang Lanfang Li Xiaomei Tang Jinyu Zeng Yige song Chao Xu Kai Zhao Guoqiang Liu Youming Lu Xinyan Li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

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