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Expression profiling of transgenes(Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds

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Journal of Integrative Agriculture 2022年第10期21卷 2818-2832页

作者： Kashif NOOR Hafiza Masooma Naseer CHEEMA Asif Ali khan Rao sohail Ahmad khan Department of Plant Breeding and Genetics University of AgricultureFaisalabad 38040Pakistan Institute of Plant Breeding and Biotechnology Muhammad Nawaz Shareef University of AgricultureMultan 60000Pakistan Center of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabad 38040Pakistan

Transgenic cotton carrying the CrylAc gene has revolutionized insect pest control since its adoption,although the development of resistance in insect pests has reduced its *** 10 years of cultivating Bacillus thuringiensis(Bt)cotton with a single Cry1 Ac gene,growers are on the verge of adopting Bt cotton that carries the double gene(Cry1 Ac+Cry2 A)due to its better effectiveness against insect ***,the current study was designed to evaluate the role of each gene in the effectiveness of Bt cotton carrying the double *** expression levels of the Cry1 Ac and Cry2 A genes were evaluated in the leaves of 10 genotypes(2 parents and 8 Fhybrids)at 30 days after sowing(DAS),while samples of leaves,bolls and flowers were taken from the upper and lower canopies at 70 and 110 *** Fhybrids were developed through reciprocal crosses between two Bt(CKC-1,CKC-2)and two non-Bt(MNH-786,FH-942)*** differential expression of transgenes was evaluated through Enzyme Linked Immuno-Sorbent Assay(ELISA).The results showed that the MNH786 xCKC-1 hybrid had the highest concentrations of Cry1 Ac gene at30 DAS(3.08μg g^(-1))and 110 DAS(1.01μg g^(-1))in *** contrast,the CKC-2 xMNH-786 hybrid showed the lowest concentrations of Cry1 Ac gene at 30 DAS(2.30μg g^(-1))and 110 DAS(0.86μg g^(-1)).The Fhybrid FH-942×CKC-2 showed the highest concentrations of Cry2 A gene at 30 DAS(8.39μg g^(-1))and 110 DAS(7.74μg g^(-1))in leaves,while the CKC-1 xMNH-786 hybrid expressed the lowest concentrations of Cry2 A gene at 30 DAS(7.10μg g^(-1))and 110 DAS(8.31μg g^(-1)).A comparison between the two stages of plant growth showed that leaves had the highest concentrations at 30 DAS,whereas the lowest concentrations were observed at 110 DAS for both genes in *** the expression pattern was compared between various plant parts in genotype CKC-2,it was found that leaves had higher concentrations of Cry1 Ac(3.12μg g^(-1))and Cry2 A(8.31μg g^(-1))at 70 DAS,followed by bolls

关键词： transgenic cotton breeding transgene expression double gene insect resistance

Bio-capped and green synthesis of ZnO/g-C_(3)N_(4) nanocomposites and its improved antibiotic and photocatalytic activities: An exceptional approach towards environmental remediation

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Chinese Journal of Chemical Engineering 2023年第4期56卷 215-224页

作者： Iltaf khan Chunjuan Wang Shoaib khan Jinyin Chen Aftab khan Sayyar Ali Shah Aihua Yuan sohail khan Mehwish KButt Humaira Asghar School of Environmental&Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212100China College of Agriculture Shihezi UniversityShihezi 832003China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Northeast Agricultural UniversityHarbin 150030China Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits and Vegetables College of AgronomyJiangxi Agricultural UniversityNanchang 330045China Department of Pharmacy University of SwabiKhyber Pakhtunkhwa 94640Pakistan Department of Physics University of Agriculture FaisalabadFaisalabad 38040Pakistan Department of Chemistry Government College University FaisalabadFaisalabad 38000Pakistan

In this research study, we have synthesized the bio-capped ZnO/g-C_(3)N_(4) nanocomposites by employing lemon juice(Citrus limon) as a stabilizer and mediator. Fruitfully, lemon juice which contains various acidic functional groups and citric acid has the capability to block the surface of g-C_(3)N_(4) from chemical reactivity and activated the surface of g-C_(3)N_(4) for various reactions. Consequently, the agglomeration behavior and controlled shape of g-C_(3)N_(4) has also been achieved. Our experimental results i.e. XRD,TEM, HRTEM, PL, FS, XPS, and PEC have confirmed that the lemon juice mediated and green g-C_(3)N_(4)(L-CN) have good performances and remarkable visible light photocatalytic activities as compared to the chemically synthesized g-C_(3)N_(4)(CN). Furthermore, the small surface area and low charge separation of g-C_(3)N_(4) is upgraded by coupling with Zn O nanoparticles. It is proved that the coupling of Zn O worked as a facilitator and photoelectron modulator to enhance the charge separation of g-C_(3)N_(4). Compared to pristine lemon-mediated green g-C_(3)N_(4)(L-CN), the most active sample 5Zn O/L-CN showed ~ 5-fold improvement in activities for ciprofloxacin(CIP) and methylene blue(MB) degradation. More specifically,the mineralization process and degradation pathways, and the mineralization process of ciprofloxacin(CIP) and methylene blue(MB) are suggested. Finally, our present novel research work will provide new access to synthesize the eco-friendly and bio-caped green g-C_(3)N_(4)nanomaterials and their employment for pollutants degradation and environmental purification.

关键词： Bio-caped green g-C_(3)N_(4) Lemon juice mediators ZnO/g-C_(3)N_(4)nanocomposite Environmental remediation Degradation pathways

Using Capsule Networks for Android Malware Detection Through Orientation-Based Features

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Computers, Materials & Continua 2022年第3期70卷 5345-5362页

作者： sohail khan Mohammad Nauman Suleiman Ali Alsaif Toqeer Ali Syed Hassan Ahmad Eleraky Deanship of Preparatory Year and Supporting Studies Computer Science DepartmentImam Abdulrahman Bin Faisal UniversityDammamSaudi Arabia National University of Computer and Emerging Sciences Pakistan Department of Computer Science Islamic University of MedinaMedinaSaudi Arabia

Mobile phones are an essential part of modern *** two popular mobile phone platforms,Android and iPhone Operating System(iOS),have an immense impact on the lives of millions of *** these two,Android currently boasts more than 84%market ***,any personal data put on it are at great risk if not properly *** the other hand,more than a million pieces of malware have been reported on Android in just 2021 till *** and mitigating all this malware is extremely difficult for any set of human *** to this reason,machine learning-and specifically deep learning-has been utilized in the recent past to resolve this ***,deep learning models have primarily been designed for image *** this line of research has shown promising results,it has been difficult to really understand what the features extracted by deep learning models are in the domain of ***,due to the translation invariance property of popular models based on ConvolutionalNeural Network(CNN),the true potential of deep learning for malware analysis is yet to be *** resolve this issue,we envision the use of Capsule Networks(CapsNets),a state-of-the-art model in deep *** argue that since CapsNets are orientation-based in terms of images,they can potentially be used to capture spatial relationships between different features at different locations within a sequence of *** design a deep learning-based architecture that efficiently and effectively handles very large scale malware datasets to detect Androidmalware without resorting to very deep *** leads tomuch faster detection as well as increased *** achieve state-of-the-art F1 score of 0.987 with an FPR of just 0.002 for three very large,real-world malware *** code is made available as open source and can be used to further enhance our work with minimal effort.

关键词： Malware security Android deep learning capsule networks

Polymorphic information and genetic diversity in Brassica speciesrevealed by RAPD markers

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BIOCELL 2020年第4期44卷 769-776页

作者： ALI RAZA ABU BAKR UMER FAROOQ WAQAR AHMAD khan AHSAN IQBAL SADETTINÇELİK MAHWISH ALI RAO sohail AHMAD khan Centre of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabad38040Pakistan Department of Environmental Sciences COMSATS Institute of Information TechnologyVehari61170Pakistan Institute of Molecular Biology&Biotechnology Bahauddin Zakariya UniversityMultan66000Pakistan Department of Plant Breeding and Genetics University of AgricultureFaisalabad38040Pakistan Department of Forestry GençVocational SchoolBingol UniversityGenç-Bingol12500Turkey Department of Biological Sciences National University of Medical SciencesRawalpindi46000Pakistan Key Lab of Biology and Genetic Improvement of Oil Crops Oil Crops Research InstituteChinese Academy of Agricultural ScienceWuhan430062China

Randomly amplified polymorphic DNA(RAPD)is a tremendously convenient approach used to discriminate between Brassica species owing to its accuracy and *** primers generate adequate genetic information that can be used in the primer-marker *** this work,twenty RAPD-PCR based markers were executed to generate polymorphic data,like polymorphic information content(PIC),mean resolving power(MRP),resolving power(RP),effective multiplex ratio(EMR),and marker index(MI)for the first time and genetic distance among and between six Brassica species were *** results indicated that 20 primers produced a total of 231 scored band and generated 87%polymorphic *** PIC,MRP,RP,MI,and EMR values were 0.088,0.65,6.7,0.78,and 8.9,*** showed an overall negative correlation with MRP,RP,MI,and EMR,whereas MRP,RP,and EMR,were positively correlated with each *** identities ranged from 41.99%(between Brassica napus and Brassica oleracea)to 68.83%(between Brassica campestris and Brassica oleracea).Dendrogram results showed no clustering between species except between Brassica campestris and Brassica ***,these results will be helpful to acquire useful information about the markers and their use to determine the genomic structures of Brassica ***,based on genetic distance and polymorphic information,new hybrids can be developed for effective oilseed production.

关键词： Brassica Dendrogram DNA markers Genetic distance Gel electrophoresis Polymorphism

Shale Gas Characterization of Sembar Formation, Khipro Area, Pakistan

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International Journal of Geosciences 2016年第8期7卷 1009-1019页

作者： Muhammad sohail khan Zainab Bibi School of Earth and Space Sciences University of Science and Technology of China Hefei China Department of Earth Sciences Quaid-i-Azam University Islamabad Pakistan

This study pertains to the evaluation of shale gas and rock physics properties of this area with respect to its total organic content of Sember Formation, Khiproarea, Pakistan. We use well logs data for this study. The Khipro area is prominent in the Lower Indus Basin for its hydrocarbon (oil and gas) structural traps. In shale gas evaluation, TOC of Sember Formation is estimated. The analysis has been done with the help of the wire line data of the well Bilal North-01. The presence of shale gas in the study area is analyzed with the help of different techniques. Rock physics and petrophysical analysis have been done in order to get the properties of the area related to the shale gas evaluation.

关键词： Shale Gas Reservoir Characterization Well Log Response in Shale Gas Methods to Compute TOC Rock Physics Model for Shale Gas

Moderate Interpretation with Attribute Analysis and 3d Visualization for Deeper Prospects of Balkassar Field, Central Potwar, Upper Indus Basin, Pakistan

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International Journal of Geosciences 2017年第5期8卷 678-692页

作者： Farhana Masood Zulfiqar Ahmad Muhammad sohail khan Quaid-i-Azam University Islamabad Pakistan Director ORIC University of Wah Wah Pakistan School of Earth and Space Sciences University of Science and Technology of China Hefei China

Balkassar is an old field with considerable remaining potential. The 2d, 3d seismic and earlier vintages show that Balkassar is composed of two folds that impart heart shaped geometry. It appears likely the early Eocene age Bhadrar formation may provide commercial production with lower water cuts from the eastern lobe (yet untapped) of the structure that may have at least 30 million barrels of unrecovered oil. Horizontal drilling may have promise as an optimum technique for recovery of oil from Paleogene reservoirs. Encouraging oil indications have also been recorded from the early Permian age tobra formation from Balkassar oxy-1 that was not tested by Oxy. Tobra sandstone reservoir can have a matrix porosity approaching 8%. When fractured recoveries from it can be relatively better than the Paleogene reservoirs. The 2D and 3D seismic acquisition has confirmed that the eastern lobe (yet untapped) of the Balkassar field is structurally higher and steeper than the Western lobe which has thus far produced over 30 million barrels. The Eastern lobe thus offers good potential for recovery of oil from the Bhadrar reservoir. The entire field is likely to have potential for recovery of oil from the early Eocene aged Tobra formation. 2-d and 3-D Seismic data interpretation, attribute analysis and visualization for deeper prospect carried in Balksasar field. Tobra and Khewra formation studied for deeper potential drilling. Time contour and depth contour map shows potential for deeper prospects. Also attribute analysis and 3d visualization show good results for deeper potential of Tobra and Khewar formations. Seismic amplitude, Reflection strength, Apparent polarity attribute are visualized and interpreted to find the potential for Tobra and Khewra formation. 3-D visualization also showed positive results for Tobra and Khewra formations.

关键词： Seismic Interpretation Structure Anticline Fracture Reservoir Attribute Analysis 3d Visualization

Structural Interpretation and Petrophysical Analysis for Reservoir Sand of Lower Goru, Miano Area, Central Indus Basin, Pakistan

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International Journal of Geosciences 2017年第4期8卷 379-392页

作者： Muhammad sohail khan Farhana Masood Qadeer Ahmed Ishtiaq Ahmed khan Jadoon Naveed Akram School of Earth and Space Sciences University of Science and Technology of China Hefei China Quaid-i-Azam University Islamabad Pakistan Oil and Gas Development Company Limited Islamabad Pakistan COMSATS Institute of Information Technology Abbottabad Pakistan

The main purpose of this research is to estimate the structural analysis and hydrocarbon potential of Miano Block by using seismic and well log techniques. Miano area hosts a number of gas fields with structural and stratigraphic traps. The area is located in Central Indus Basin which is a part of an extensional regime exhibiting normal faulting due to the split of the Indian Plate firstly from Africa and then from Madagascar and Seychelles. Miano area recognized as a proven petroleum province which has complex tectonic history of Cretaceous extensional and overprints of Tertiary strike-slip tectonics. The area has prospect with accumulation of hydrocarbons in structural and stratigraphic traps including pinchouts. NW-SE oriented Khairpur and Mari Highs are main structural features with impact on the fault system. The sands of Lower Goru of Lower Cretaceous age are acting as a reservoir in the area. The area has great potential of hydrocarbons for which more exploratory wells are required to be drilled with better insight of structural and stratigraphic traps.

关键词： Seismic Interpretation Time-and-Depth Contour Map 3D View Synthetic Seismograph Reservoir Estimation

Effects of different planting systems on yield of potato crop in Kaghan Valley: A mountainous region of Pakistan

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Agricultural Sciences 2013年第4期4卷 175-179页

作者： Muhammad Qasim Salma Khalid Alia Naz Muhammad Zafarullah khan sohail Ahmad khan Department of Agriculture Extension and Communication Agricultural University Peshawar Peshawar Pakistan Department of Environmental Sciences Abdul Wali Khan University Mardan Mardan Pakistan

Conversion of potato from conventional methods to wide bed planting systems may increase water and nitrogen use efficiency in commercial potato production system by reducing the amount of irrigation water and water applied nitrogen fertilizer bypassing the potato root zone. Potato (Solanum tuberosum L) cv. Desiree was tested against different planting system for yield and yield components at Kaghan, a high mountainous Himalayan region ofPakistan. The experiment was carried out at Himalayan Agricultural Research Station (HARS), Kaghan during the summer season of 2005. The results showed that maximum tuber growth (88.7%), number of stems per plant (3.5), plant expansion (45.5 cm), average number of tubers per plant (10.1) and yield per hectare (12.4 t/ha) were significantly different and higher when potatoes were planted on wide bed and covered with soil from one side. Tallest plants (53.4 cm) were observed when potatoes were sown on the ridges. Maximum number of green potatoes (12.5) and injured potatoes (5.3%) were observed when the tubers were planted following local farmers’ method. Keeping in view the soil type, land slopping, we recommend sowing potatoes on relatively plain wide beds and covering it with soil from one side, for potato cultivation in the area.

关键词： Potato (Solanum tuberosum L) Planting Systems Yield Response Kaghan Valley Pakistan

Comprehensive Probabilistic Seismic Hazard Analysis of Makran Subduction Zone along the Costal Area of Pakistan

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Comprehensive Probabilistic Seismic Hazard Analysis of Makra...