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Tuberculosis:an experience from Mycobacterium smears and culture analysis

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Asian Pacific Journal of Tropical Medicine 2009年第2期2卷 47-53页

作者： Zeehaida M siti asma h siti hawa h Zaidah AR Norbanee Th Department of Medical Microbiology and Parasitology School of Medical SciencesUniversiti Sains MalaysiaKubang KerianKelantanMalaysia

Objective:Simple tests like direct smear of the acid fast bacilli(AFB) and Mycobacterium culture could assist the diagnosis of *** study is aimed at reviewing the outcome of smears,culture results and contamination rate among specimens requested for AFB smear and Mycobacterium ***:Retrospective laboratory data analysis requesting for Mycobacterium culture from January 2005 till December 2006 was done in a tertiary teaching hospital of Universiti Sains Malaysia,Kubang Kerian,Kelantan,***: Four hundred and sixty seven(36.6%) isolates grew from 1 277 *** these isolates,314 (67.2%) grew Mycobacterium tuberculosis,23(4.9%) grew Mycobacterium other than tuberculosis and 38 (8.1%) grew *** the *** cultures,165(52.5%) had growth of more than 100 confluent colonies,whereas 39 cultures(12.4%) had growth of less than 19 *** smear for AFB among smear positive cases showed presence of more than 50 bacilli/line in 231(49.5%) cases and smear negative cases accounted for 63(13.5%).Among smear positive cases,291(94.5%) cultures grew Mycobacterium species and another 17(5.5%) cultures grew *** smear negative cases,32(62.7%) cultures grew Mycobacterium species and 19(37.3%) cultures grew ***:The results from data analysis of the Mycobacterium cultures should be critically utilized in order to review the laboratory performance and to improve its services in the *** of the data is also useful to the administrators of the hospital in terms of estimating the risk of occupational hazard faced by the health care workers.

关键词： Tuberculosis Mycobacterium tuberculosis Culture Acid fast bacilli Smear Contamination

In vitro study of natural plant products against oral bacteria

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Asian Pacific Journal of Tropical Medicine 2009年第6期2卷 18-21页

作者： Siddiqui R siti asma'h Tang L Jie CC Rosliza AR Roziawati Y School of Dental Sciences Universiti Sains Malaysia Kubang KerianKelantanMalaysia Department of Medical Microbiology and Parasitology School of Medical SciencesUniversiti Sains MalaysiaKubang KerianKelantanMalaysia

Objective:To analyze the effect of selected plant product against several bacterial which commonly causes oral *** was hope that in future,this product will become the remedy for treatment of oral infection and with the hope that it can substitute ***:A total of 5 species of oral bacteria from American Type Culture Collection(ATCC) were employed in this study(***,***,***,*** and *** ).Three types of natural plants crude extracts were used(garlic,curry leaves and cloves).Bactericidal and bacteriostatic effects of these herbs were ***:It was shown garlic had antibacterial effects on all *** Minimal Inhibitory Concentration(MIC in g/mL) of garlic towards S. aureus,***,***,*** and *** were 0.3,1.8,1.2,0.5 and 1.8,respectively. There was significant difference among the MIC of garlic on tested *** was more potent toward ***. The curry leaf solution on the other hand,did not show any zone of inhibition in all bacteria plates but adversely showed enhanced growth of those *** had shown its antibacterial effects on *** and *** clove was more potent toward *** with the MIC of 0.45 g/*** was more sensitive to clove compared to *** ***,it was more sensitive to garlic compared to clove. Conclusion:The antibacterial activity of garlic and clove crude extracts shown in our study further confirm these natural plants’potential usage in therapeutic use for oral diseases or *** could be the platform for the interested party to do research and development on it and to produce oral health products which are more affordable for lower economic income groups and with fewer side effects as seen in synthetic drug.

关键词： Garlic Clove Curry leaves MIC Antibacterial effect

Unsatisfactory Knowledge, Attitude and Practice on Leptospirosis among Army Personnel’s in Northeastern Malaysia

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health 2020年第3期12卷 281-292页

作者： siti Sara Y. Aziah B. D. Azwany Y. N. Mohd Nazri S. Zahiruddin W. M. Nabilah A. siti asma’ h. Zaliha I. Public health Division Selangor State Health Department Ministry of Health Selangor Malaysia Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Department of Medical Microbiology & Parasitology School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Population health & Preventive Medicine Discipline Faculty of Medicine Universiti Teknologi MARA Selangor Malaysia.

Leptospirosis is a re-emerging zoonotic disease caused by infection of the bacteria belongs to Genus leptospira. It occurs in tropical, subtropical and temperate zones. It is also known as occupational-related disease as certain occupations are associated with the occurrence of leptospirosis. Army is identified as one of the high-risk occupations in contracting leptospirosis. This study was done to determine knowledge, attitude and practice (KAP) among army personnel on leptospirosis. A cross sectional study was conducted among 616 army personnel in four based camps in Northeastern Malaysia, who fulfilled the inclusion and exclusion criteria. A validated KAP questionnaire was used, consisting of knowledge, attitude and practice questions. More than half (52.8%) of the respondents had poor knowledge score. For the attitude score, almost the same proportions had satisfactory attitude. The same finding was seen in practice score, whereby those with good practice (54.9%) and bad practice (45.9%). In conclusion, the finding showed that almost all army personnel had unsatisfactory KAP, thus, it is recommended for the army to have a good health education program on leptospirosis among army personnel to protect the army personnel from risk of getting leptospirosis.

关键词： Army Personnel Leptospirosis Knowledge Attitude Practice

Do Occupational Factors Render Army Service a high-Leptospirosis-Risk Occupation?

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Occupational Diseases and Environmental Medicine 2020年第2期8卷 79-89页

作者： siti Sara Y. Aziah B. D. Azwany Y. N. Mohd Nazri S. Zahiruddin W. M. Nabilah A. siti asma’ h. Zaliha I. Fairuz A. Public health Division Selangor State Health Department Ministry of Health Selangor Malaysia Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Department of Medical Microbiology & Parasitology School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia Population health & Preventive Medicine Discipline Faculty of Medicine Universiti Teknologi MARA Selangor Malaysia Institute for Medical Research (IMR) Kuala Lumpur Malaysia

Objective: Leptospirosis is a known zoonotic disease which is closely related to certain high-risk occupational group. This study aimed to identify the risk factors associated with seropositive leptospirosis among army personnel. Methodology: A cross sectional study was conducted involving 616 army personnel using a self-administered questionnaire. The questionnaire consisted of sociodemographic, occupational and environmental data as well as recreational activities history. Serum sample for leptospirosis was taken for screening with IgM Enzyme-linked Immunosorbent Assay (IgM ELISA) test. Positive tests were confirmed by Microscopic Agglutination Test (MAT). Results: All respondents were male, Malays and married with the mean age of 29.28 (7.15) years old. The mean duration of working was 11.50 (7.75). Majority of the respondents were non-officer and riflemen, smokers (455 (73.9%), stayed inside the camp and engaged in recreational activities. The mean duration of employment was 11.50 (7.75) years. The overall seroprevalence of leptospirosis was 16.2% (95% CI: 13.32, 19.15) and the predominant pathogenic serovar identified was Terengganu (38.2%). Those who had lower educational levels were more protective for positive leptospirosis compared to those who have only primary education (OR: 0.32;95% CI: 0.14, 0.76;p = 0.009). Conclusion: Despite being named as one of the high-risk occupations for leptospirosis, this study found no significant occupational factors related to leptospirosis seropositivity among army personnel.

关键词： Army Leptospirosis Seroprevalence Igm Enzyme-Linked Immunosorbent Assay (ELISA) Microscopic Agglutination Test (MAT)

Arsenic variation in soil layers in Bandung Regency,Indonesia:A statistical approach for identifying potential sources

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Emerging Contaminants 2024年第3期10卷 193-204页

作者： Nurul Fahimah Katharina Oginawati Indah Rachmatiah siti Salami Dasapta Erwin Irawan Diyah Anggraeni hirundini Rustica Absari Utriweni Mukhaiyar Udjianna Sekteria Pasaribu Kurnia Novita Sari Lira Adiyani Environmental Management Technology Research Group Department of Environmental EngineeringFaculty of Civil and Environmental EngineeringInstitut Teknologi BandungJl.Ganesha 10Bandung40132West JavaIndonesia Department of Geological Engineering Faculty of Earth Sciences and TechnologyInstitut Teknologi BandungJl.Ganesha 10Bandung40132West JavaIndonesia Department of Environmental Engineering Faculty of Civil and Environmental EngineeringInstitut Teknologi BandungJl.Ganesha 10Bandung40132West JavaIndonesia Statistics Expertise Group Faculty of Mathematics and Natural SciencesInstitut Teknologi BandungJl.Ganesha 10Bandung40132West JavaIndonesia Technical Implementation Unit for Groundwater Ministry of Public Works and Housing of IndonesiaJl.Ir.H.Juanda No.193Bandung40135Indonesia

This research investigates the arsenic concentrations in 96 soil samples from a regency in Indonesia using a statistical *** samples were collected from three depth layers(topsoil[10e20 cm],subsoil[50e60 cm],and deep soil[90e100 cm]),with each layer consisting of 32 *** analysis using SPSS was employed to analyze potential arsenic sources based on the proximity of study locations to geothermal system areas,land use,distance from industry,and the presence of study locations on fault *** findings indicate that the potential source of arsenic contamination in the soil are suspected to be associated with geothermal system activities and the presence of soil samples on fault *** arsenic concentration in locations near to the geothermal system(5-10 km)was 45 times higher(OR=45,95%CI=45e362.57,phigher in study areas situated on fault zones(OR=3.828,95%CI=1.507e9.719,phe research suggests that arsenic in the soil may also originate from anthropogenic activities,such as agriculture and industry,as the highest concentrations were found in subsoil or topsoil layers compared to deep *** variation in arsenic concentration from highest to lowest in agricultural soil layers was subsoil>topsoil>deep soil,whereas in residential soil,it was subsoil>deep soil>*** arsenic concentration variation at near distances from industrial areas was topsoil>subsoil>deep soil,contrasting with medium to far distances from industry(subsoil>deep soil>topsoil).Therefore,these findings can be utilized to prioritize remediation efforts and provide input for future research on arsenic contamination in the region.

关键词： Anthropogenic sources Arsenic sources Indonesian soil Natural sources

New generation Amberlite XAD resin for the removal of metal ions: A review

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Journal of Environmental Sciences 2015年第5期27卷 104-123页

作者： Akil Ahmad Jamal Akhter Siddique Mohammad Asaduddin Laskar Rajeev Kumar siti hamidah Mohd-Setapar asma Khatoon Rayees Ahmad Shiekh Centre of Lipids Engineering & Applied Research (CLEAR) Faculty of Chemical Engineering Universiti Teknologi Malaysia Department of Materials Engineering and Chemistry Faculty of Civil Engineering Czech Technical University Department of Chemistry Jazan University Department of Environmental Sciences Faculty of Meteorology Environment and Arid Land Agriculture King Abdulaziz University Department of Chemistry Faculty of Science Taibah University

The direct determination of toxic metal ions, in environmental samples, is difficult because of the latter＇s presence in trace concentration in association with complex matrices, thereby leading to insufficient sensitivity and selectivity of the methods used. The simultaneous removal of the matrix and preconcentration of the metal ions, through solid phase extraction,serves as the promising solution. The mechanism involved in solid phase extraction（SPE）depends on the nature of the sorbent and analyte. Thus, SPE is carried out by means of adsorption, ion exchange, chelation, ion pair formation, and so forth. As polymeric supports, the commercially available Amberlite resins have been found very promising for designing chelating matrices due to its good physical and chemical properties such as porosity, high surface area,durability and purity. This review presents an overview of the various works done on the modification of Amberlite XAD resins with the objective of making it an efficient sorbent. The methods of modifications which are generally based on simple impregnation, sorption as chelates and chemical bonding have been discussed. The reported results, including the preconcentration limit, the detection limit, sorption capacity, preconcentration factors etc., have been reproduced.

关键词： Amberlite XAD Toxic metals Chelating resin Preconcentration Sorption capacity

Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats

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Journal of Integrative Medicine 2019年第1期17卷 66-70页

作者： Che Badariah Abd Aziz siti Qusyasyiah Ahmad Suhaimi hidani hasim asma hayati Ahmad Idris Long Rahimah Zakaria Department of Physiology School of Medical Sciences Health Campus Universiti Sains Malaysia School of health Sciences Health Campus Universiti Sains Malaysia

Objective: This study was done to determine whether Tualang honey could prevent the altered nociceptive behaviour, with its associated changes of oxidative stress markers and morphology of the spinal cord,among the offspring of prenatally stressed ***: Pregnant rats were divided into three groups: control, stress, and stress treated with Tualang honey. The stress and stress treated with Tualang honey groups were subjected to restraint stress from day 11 of pregnancy until delivery. Ten week old male offspring(n = 9 from each group) were given formalin injection and their nociceptive behaviours were recorded. After 2 h, the rats were sacrificed, and their spinal cords were removed to assess oxidative stress activity and morphology. Nociceptive behaviour was analysed using repeated measures analysis of variance(ANOVA), while the levels of oxidative stress parameters and number of Nissl-stained neurons were analysed using a one-way ***: This study demonstrated that prenatal stress was associated with increased nociceptive behaviour, changes in the oxidative stress parameters and morphology of the spinal cord of offspring exposed to prenatal stress; administration of Tualang honey reduced the alteration of these ***: This study provides a preliminary understanding of the beneficial effects of Tualang honey against the changes in oxidative stress and neuronal damage in the spinal cord of the offspring of prenatally stressed rats.

关键词： Prenatal stress Nociceptive responses Tualang honey Spinal cord

Text Extraction with Optimal Bi-LSTM

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Computers, Materials & Continua 2023年第9期76卷 3549-3567页

作者： Bahera h.Nayef siti Norul huda Sheikh Abdullah Rossilawati Sulaiman Ashwaq Mukred Saeed Computer Techniques Engineering Department Ibn Khaldun University CollegeBaghdad10011Iraq Faculty of Information Science and Technology Universiti Kebangsaan MalaysiaBangiSelangor43600Malaysia School of Electrical Engineering and Artificial Intelligence Xiamen University MalaysiaSepang43900Malaysia

Text extraction from images using the traditional techniques of image collecting,and pattern recognition using machine learning consume time due to the amount of extracted features from the *** Neural Networks introduce effective solutions to extract text features from images using a few techniques and the ability to train large datasets of images with significant *** study proposes using Dual Maxpooling and concatenating convolution Neural Networks(CNN)layers with the activation functions Relu and the Optimized Leaky Relu(OLRelu).The proposed method works by dividing the word image into slices that contain *** pass them to deep learning layers to extract feature maps and reform the predicted *** Short Memory(BiLSTM)layers extractmore compelling features and link the time sequence fromforward and backward directions during the training *** Connectionist Temporal Classification(CTC)function calcifies the training and validation loss *** addition to decoding the extracted feature to reform characters again and linking them according to their time *** proposed model performance is evaluated using training and validation loss errors on the Mjsynth and Integrated Argument Mining Tasks(IAM)*** result of IAM was 2.09%for the average loss errors with the proposed dualMaxpooling and *** the Mjsynth dataset,the best validation loss rate shrunk to 2.2%by applying concatenating CNN layers,and Relu.

关键词： Deep neural network text features dual max-pooling concatenating convolution neural networks bidirectional long short memory text connector characteristics

Surface hydrophobicity: effect of alkyl chain length and network homogeneity

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Frontiers of Chemical Science and Engineering 2021年第1期15卷 90-98页

作者： Wenqian Chen Vikram Karde Thomas N.h.Cheng siti S.Ramli Jerry Y.Y.heng Department of Chemical Engineering Imperial College LondonLondonSW72AZUK Department of Food Technology Faculty of Applied SciencesUniversiti Teknologi MARA(UiTM)Shah AlamSelangorMalaysia

Understanding the nature of hydrophobicity has fundamental importance in environmental *** spherical silica nanoparticles(diameter=369±7 nm)as the model material,the current study investigates the relationship between the alkyl chain network and *** alkyl silanes with different chain length(triethoxymethylsilane(C1)***(octyl)silane(C8))were utilised separately for the functionalisation of the *** contact angle and inverse gas chromatography results show that the alkyl chain length is essential for controlling hydrophobicity,as the octyl-functionalised nanoparticles were highly hydrophobic(water contact angle=150.6°±6.6°),whereas the methyl-functionalised nanoparticles were hydrophilic(i.e.,water contact angle=0°,similar to the pristine nanoparticles).The homogeneity of the octyl-chain network also has a significant effect on hydrophobicity,as the water contact angle was reduced significantly from 148.4°±3.5°to 30.5°±1.0°with a methyl-/octyl-silane mixture(ratio=160:40µL·g^(–1) nanoparticles).

关键词： hydrophobicity surface energy wettability alkyl chain network silica nanoparticle