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Rooting of Stem Cuttings with Different Indole 3 Butyric Acid (IBA) Treatments and Development of Micropropagation Protocol for <i>Piper betle</i>L. Node Culture

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American Journal of Plant Sciences 2017年第12期8卷 3084-3100页

作者： Qusay Abdulhamza Muttaleb Thohirah Lee Abdullah Azmi Abdul Rashid siti aishah hassan Technical Institute of Babel Al-Furat Al-Awsat Technical University Al Hillah Iraq Department of Crop Science Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia Laboratory of Plant Microstructure and Anatomy Department of Crop Science Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia In Vitro Laboratory Department of Agriculture Technology Universiti Putra Malaysia Serdang Malaysia

The present study, conducted during 2016 and 2017 seasons, aimed to investigate the effect of IBA on rooting of Piper betle L. stem cuttings (softwood and semi-hardwood). The experiment was undertaken in misting house field 2 UPM using the sand media to determine the adventitious roots initiation and development using the histological method. The cuttings were treated with different IBA concentrations (0, 500, 1000, 1500 and 2000 mg/L). The nodes explants were used in the development of a protocol for in vitro propagation of P. betle L., with different concentrations of Clorox with different times of immersion (20% Clorox 10 minutes, 30% Clorox 10 minutes, 20% Clorox 20 minutes, and 30% 20 minutes). In multiplication of the plantlets, Murashige and Skoog (MS) medium with different concentrations of BAP (0, 0.5, 1.0, 2.0 mg/L) were used to investigate the rooting of the explants. The results indicated that the types of the cuttings were different in the rooting capacity and the length of the roots. Moreover, it was found that in comparison with the control treatment, by a rise in the concentrations of the IBA, there was a significant upsurge in the rooting percentage, the root diameter, and the number of the roots. The results indicated that the types of cutting with 1000, 1500 and 2000 mg/L IBA perform better in the root percentage (100%) in the semi hardwood cuttings. The best results, however, were 2000 mg/L IBA in the semi hardwood cuttings, with the number of the roots to be 35.05, and the fresh weight of the roots to be 3.94 g, the dry weight of the roots to be 0.33 g, the length of the roots to be 391.88 cm, the roots diameter to be 1.21 mm, the surface area of the roots to be 121.83 cm2, and the root volume to be 2.99 cm3. Nonetheless, the optimal concentration of Clorox with the time immersion was 20% with the 20-minute immersion time, which produced a shoot induction percentage of 30% dead explants and a mean number of 70.00 shoots per explant and the optim

关键词： Piper betle Rooting Stem Cutting Indole-3-Butyric Acid Micropropagation

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Synthesis, Characterization and X-ray Structure of N-(4-bromobutanoyl-N'-(2-3-and 4-methylphenyl)thiourea

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Chinese Journal of Structural Chemistry 2015年第3期34卷 379-385页

作者： Hamza M Abosadiya siti aishah Hasbullah Bohari M Yamin School of Chemical Sciences and Food Technology Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia

N-4-（bromobutanoyl）-N＇-（o-, m- and p-tolyl）thioureas 3a, 3b and 3c respectively, were synthesized by the reaction of 4-bromobutanoylisothiocyanate with p-, m- and o-toludine. The products were characterized by IR, and NMR spectroscopic techniques. The two carbonoylthiourea isomers N-（4-bromobutanoyl）-N＇-（3-methylphenyl）thiourea （3b） and N-（4-bromobutanoyl）-N＇-（4- methylphenyl）thiourea （3c） were obtained in crystalline form by recrystallization from DMSO. X- ray crystallographic studies showed that both compounds 3b and 3c crystallize in triclinic system with space group ofPi. The molecules adopt trans-cis configuration with respect to the positions of 4-bromobutanoyl and tolyl groups respectively, against the thiono C=S bond across their C-N bonds The configuration is attributed by the intrahydrogen bond between the carbonyl oxygen and amide hydrogen atoms. Both crystal structures are stabilized by N-H…S intermolecular hydrogen bonds to form dimers and arranged along the b axis.

关键词： 4-bromobutyryl chloride, thiourea derivatives, X-ray structural study

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Latent tuberculosis infection among medical students in Malaysia

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Asian Pacific Journal of Tropical Medicine 2019年第4期12卷 181-184页

作者： Maha Abdullah Ummi Nadira Daut siti aishah Daud Nor Afifi Mohd Romli Marsitah Abdul Jalil Noorelina Muhammad Safarina Mohammad Ismuddin Masriana hassan Department of Pathology Faculty of Medicine and Health Sciences Universiti Putra Malaysia

Objective: This study aimed to determine prevalence of latent tuberculosis infection among medical students and tuberculosis exposure at the health facilities. Methods: A cross-section of study year 1(n=68) and year 5(n=75) medical students in a local university were recruited for latent tuberculosis infection testing using QuantiFERON-TB Gold Plus and a questionnaire analyzed for multivariate risk. Results: The majority of the study were vaccinated with BCG. None of year 1 medical students were positive for latent tuberculosis infection, however, six(8.0%) year 5 students were tested positive for latent tuberculosis infection. A higher incidence of year 5 medical students claimed to be exposed to tuberculosis at health facility(65.3% vs. 4.4%) and a higher percentage reported contact with tuberculosis case over the preceding year compared to year 1 students(30.7% vs. 8.8%). Conclusion: We observed a higher incidence of latent tuberculosis infection and higher exposure to tuberculosis in health facilities among year 5 medical students. Baseline screening and monitoring for progression to tuberculosis infection may benefit tuberculosis management programs.

关键词： Latent tuberculosis infection Medical students Interferon-gamma release assay

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mRNA transcriptome profiling of human hepatocellular carcinoma cells HepG2 treated with Catharanthus roseus-silver nanoparticles

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World Journal of Hepatology 2023年第3期15卷 393-409页

作者： Nur Asna Azhar siti aishah Abu Bakar Marimuthu Citartan Nor Hazwani Ahmad Department of Biomedical Science Advanced Medical and Dental InstituteUniversiti Sains MalaysiaKepala Batas 13200Pulau PinangMalaysia Liver Malignancies Research Program Advanced Medical and Dental InstituteUniversiti Sains MalaysiaKepala Batas 13200Pulau PinangMalaysia Faculty of Bioresources and Food Industry Universiti Sultan Zainal AbidinBesut CampusBesut 22200TerengganuMalaysia

BACKGROUND The demand for the development of cancer nanomedicine has increased due to its great therapeutic value that can overcome the limitations of conventional cancer ***,the presence of various bioactive compounds in crude plant extracts used for the synthesis of silver nanoparticles(AgNPs)makes its precise mechanisms of action *** To assessed the mRNA transcriptome profiling of human HepG2 cells exposed to Catharanthus roseus ***(***)-*** The proliferative activity of hepatocellular carcinoma(HepG2)and normal human liver(THLE3)cells treated with *** were measured using MTT *** RNA samples were extracted and sequenced using BGIseq500 *** is followed by data filtering,mapping,gene expression analysis,differentially expression genes analysis,Gene Ontology analysis,and pathway *** The mean IC 50 values of *** on HepG2 was 4.38±1.59μg/mL while on THLE3 cells was 800±1.55μg/*** profiling revealed an alteration of 296 *** induced the expression of stress-associated genes such as MT,HSP and *** signalling pathways were potentially activated through MAPK,TNF and TGF pathways that are responsible for apoptosis and cell cycle *** alteration of ARF6,EHD2,FGFR3,RhoA,EEA1,VPS28,VPS25,and TSG101 indicated the uptake of ***-AgNPs via both clathrin-dependent and clathrinindependent *** This study provides new insights into gene expression study of biosynthesised AgNPs on cancer *** cytotoxicity effect is mediated by the aberrant gene alteration,and more interestingly the unique selective antiproliferative properties indicate the *** as an ideal anticancer candidate.

关键词： Catharanthus roseus HepG2 Silver nanoparticles Transcriptome oxidative stress Apoptosis Cell cycle

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Eco-friendly method for synthesis of zeolitic imidazolate framework 8 decorated graphene oxide for antibacterial activity enhancement

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Particuology 2020年第2期18卷 24-32页

作者： Nazerah Ahmad Nik Abdul Hadi Md Nordin Juhana Jaafar Nik Ahmad Nizam Nik Malek Ahmad Fauzi Ismail Muhammad Nabil Fikri Yahya siti aishah Mohd Hanim Mohd Sohaimi Abdullah Advanced Membrane Technology Research Centre Universiti Teknologi Malaysia81310 SkudaiJohorMalaysia Malaysian Institute of Chemical Engineering Technology-Universiti Kuala Lumpur Lot 1988Bandar VendorTaboh Naning78000 Alor GajahMelakaMalaysia Chemical Engineering Department Universiti Teknologi PETRONAS32610 Seri IskandarPerakMalaysia Department of Biosciences Faculty of ScienceUniversiti Teknologi Malaysia81310 SkudaiJohorMalaysia Centre for Student Innovation and Technology Entrepreneurship Universiti Teknologi Malaysia81310 SkudaiJohorMalaysia

We report a rapid method for synthesis of zeolitic imidazolate framework 8(ZIF-8)-decorated graphene oxide(GO)composites(ZGO)with good antibacterial *** ZGO composites were synthesized at room temperature with low GO to metal salt *** samples were characterized by X-ray diffraction,transmission electron microscopy,Fourier transform infrared spectroscopy,thermal gravimetric analysis,and surface area *** characterization results show that ZIF-8 with a size of approximately 120 nm is successfully decorated on the surface of GO sheets with the host ZIF-8 framework maintained in the synthesized composite,but there is a significant reduction in the Brunauer-Emmett-Teller surface *** antibacterial activities of the samples against Escherichia coli ATCC 11229 and Staphylococcus aureus ATCC 6538 as model strains of gram-negative and-positive bacteria,respectively,were determined by disc diffusion and minimum inhibitory concentration(MIC)***-1.0(1 wt%of ratio of GO to metal salt)shows the highest antibacterial activity with MIC values required to inhibit bacterial growth of *** and *** of 5 times lower than those of pristine *** antibacterial mechanisms are proposed based on field-emission scanning electron microscope images of the two bacteria after contact with the synthesized ***,owing to the simple synthesis,good stability,low chemical usage,and excellent antibacterial activity of the ZGO composites,they show great potential for application in the field of microbial contamination control.

关键词： Graphene oxide Metal-organic framework Hybrid nanocomposite Zeolitic imidazolate framework Antibacterial agent

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Influence of Solvent Polarity and Conditions on Extraction of Antioxidant, Flavonoids and Phenolic Content from A verrhoa bilimbi

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Journal of Food Science and Engineering 2014年第5期4卷 255-260页

作者： Norlia Muhamad siti aishah Muhmed Mashitah M. Yusoff Jolius Gimbun Faculty of Bioresources & Food Industry Universiti Sultan Zainal Abidin Tembila Campus Besut 22200 Terengganu Malaysia Faculty of Chemical and Natural Resources Engineering Univ. Malaysia Pahang Gambang 26300 Malaysia Faculty of Industrial Science and Technology Univ. Malaysia Pahang Gambang 26300 Malaysia Centre of Excellence for Advanced Research in Fluid Flow (CARIFF) Univ. Malaysia Pahang Gambang 26300 Malaysia

This paper presents the influence of solvent polarity and extraction conditions on the extraction of total flavonoid, total phenolic and antioxidants from Averrhoa bilimbi. The experiment was performed using a different solvent at different extraction conditions, including extraction time （15-240 min）, temperature （30-70 ℃） and agitation speed （50-300 rpm）. Results showed that yields of extraction varies with solvent polarity. Extraction using 50% aqueous methanol gives the highest antioxidant activity and flavonoid content. The highest total flavonoid content （193.3 μg quercetin equivalent/g dry weight）, total phenolic content （717.8 μg gallic acid equivalent/g dry weight） and antioxidant activity （77%） was achieved using 50% methanol, at 70 ℃ and agitation speed of 300 rpm. This work may be useful for obtaining higher bioactive compounds during the extraction process of A. bilimbi.

关键词： Extraction flavonoid belimbing buluh phenolic antioxidant.

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Descriptive assessment on diabetic retinopathy screening in an awareness programme in Malaysia

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International Journal of Ophthalmology(English edition) 2020年第11期13卷 1808-1813页

作者： Nor Fariza Ngah Nor Asiah Muhamad Zalifa Zakiah Asnir Roslin Azni Abdul Aziz Zaidah Mhad Kassim siti aishah Sahar Nor Azita Ahmad Tarmidzi Chan Li Yen Rosmawati Uthman Narjis Satar Noor Zahirah Husain Azian Adnan John Mathen Kayalakakathu Bariyah Kadas Noor Suriani Mohamad Shaiful Azlan Kassirn Department of Ophthalmology Shah Alam HospitalSelangor 40000Malaysia Sector for Evidence Based Healthcare National Institutes of HealthMinistry of HealthSelangor 40170Malaysia Institute for Public Health National Institutes of HealthMinistry of HealthSelangor 40170Malaysia Department of Ophthalmology Ampang HospitalSelangor 68000Malaysia Department of Ophthalmology Serdang HospitalSelangor 43000Malaysia Department of Ophthalmology Putrajaya HospitalFederal Territory 62250Malaysia Department of Ophthalmology Kuala Lumpur HospitalFederal Territory 50586Malaysia Department of Ophthalmology Tengku Ampuan Rahimah HospitalSelangor 41200Malaysia Department of Ophthalmology Selayang HospitalSelangor 68100Malaysia Department of Ophthalmology Sungai Buloh HospitalSelangor 47000Malaysia Selangor State Health Department Ministry of HealthSelangor 40100Malaysia

AIM:To determine the prevalence of diabetic retinopathy(DR)among diabetic patients at the primary health clinics in Selangor,***:All diabetic patients were screened in Retinal Disease Awareness Programme(RDAP)and those who had significant DR changes were referred to the hospital for further *** analyses were done to determine the prevalence of DR and sociodemographic characteristics among patients with *** and multivariable analysis using Logistic regression were performed to find association and predictor factors in this ***:A total of 3305 patients aged 40y and above were screened for *** patients screened,9%patients were found to have DR and other visual complication such as maculopathy(0.9%),cataract(4.8%)and glaucoma(0.4%).The mean age of patients without retinopathy was 57.82±8.470y and the mean age of patients with DR was 63.93±*** 61.5%of the patients screened were aged below 60y and 38.5%were aged 60y and *** of the patients screened were women 58.5%and Malay in the age group of 50-59y,while 27%were aged *** association were found between age,sex,race,visual loss and ***:Although the prevalence of DR among patients is not alarming,effective interventions need to be implemented soon to avert a large burden of visual loss from DR.

关键词： diabetic retinopathy retinal disease awareness programme eye screening Selangor

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Finite Difference Method for Solving Material Balance Equation in Lithium-Ion Cell

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材料科学与工程（中英文版） 2010年第11期4卷 94-99页

作者： Yusof Norzihani Hashim Ali siti aishah Arof Abdul Kariem Faculty of Information and Communication Technology Universiti Teknikal Malaysia Melaka Hang Tuah Jaya Durian Tunggal76109 Melaka Malaysia Institute of Mathematical Sciences Faculty of Science University of Malaya Kuala Lumpur 50603 Malaysia Department of Physics Faculty of Science University of Malaya Kuala Lumpur 50603 Malaysia

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