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Storage of Campomanesia adamantium(Cambess.)*** Seeds:Influence of Water Content and Environmental Temperature

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American Journal of Plant Sciences 2014年第17期5卷 2555-2565页

作者： Daiane Mugnol Dresch silvana de paula quintao scalon Tathiana Elisa Masetto Rosilda Mara Mussury Pos-Doutoranda em Agronomia(PNPD/CAPES) Universidade Federal da Grande Dourados(UFGD)DouradosBrasil Faculdade de Ciencias Agrarias Universidade Federal da Grande Dourados(UFGD)DouradosBrasil Faculdade de Ciencias Biologicas e Ambientais Universidade Federal da Grande Dourados(UFGD)DouradosBrasil

The present work evaluated the conservation of Campomanesia adamantium seeds under different conditions. The fruits used in the study were collected from matrices located in the city of Ponta Porã-MS, Brazil. After processing, the seeds were slowly dried to different water content levels and subsequently exposed to various environmental conditions: 25°C ± 2°C, 35% relative humidity (laboratory);16°C ± 1°C, 40% relative humidity (cold and dry);8°C ± 1°C, 35% relative humidity (refrigeration);and -18°C ± 1°C, 42% relative humidity (freezing). Each treatment was conducted for 0 (recently processed seeds, with superficial drying of 40 minutes), 30, 60, 90, 120, 150, and 180 days. Seed potential was physiologically evaluated based on radicle protrusion, percentage of normal seedlings, seedling length (primary root, shoot and total), and dry mass of the seedlings. The experimental design was a completely randomized factorial scheme with split-split plots (4 temperatures/environments × 5 water content × 7 storage periods). C. adamantium seeds tolerated a reduction in the water content to 15.3% but did not tolerate the storage period, confirming the recalcitrant behavior of the seeds.

关键词： Myrtaceae Brazilian Savanna Drying Conservation

Gas Exchanges and Antioxidant Activity in Copaifera langsdorffii *** after Flooding

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American Journal of Plant Sciences 2018年第5期9卷 979-994页

作者： derek Brito Chaim Jardim Rosa silvana de paula quintao scalon Thais Cremon Daiane Mugnol Dresch Faculty of Agricultural Sciences Federal University of Grande DouradosState Mato Grosso do SulBrazil Cnpq Productivity Scholarship Faculty of Agricultural SciencesFederal University of Grande DouradosState Mato Grosso do SulBrazil

The Copaifera langsdorffii desf. seedlings, popularly known as “copaiba” or diesel tree, present a natural occurrence in the riparian forests of the Brazilian Cerrado, however, not much is known about their capacity to recover from flooding, particularly when the focus is on their establishment and use in programs for the recovery of degraded areas near watercourses and subject to flooding. The objective of this study was to evaluate over time the recovery of the efficiency of the photosynthetic apparatus and the antioxidant activity of C. langsdorffii seedlings flooded during different periods. The pots with the seedlings were placed in a plastic tank and submerged with water, and were removed from the flood condition every 21 days (0, 21, 42, 63 and 84 days), and evaluated every 30 days (0, 30, 60, 90 and 120 days). The seedlings tolerated the flooding for up to 42 days, recovering after suspension of this condition. Seedlings flooded for 63 and 84 days showed lower recovery capacity after flooding, independently of the evaluation period. Under these conditions, lower efficiency of the photosynthetic apparatus, gas exchange, photo assimilate production and seedling quality were observed.

关键词： Water Stress Photosynthesis Chlorophyll A Fluorescence Antioxidant Enzymes

Reduced Sensitivity of Campomanesia adamantium(Cambess.)*** Seeds to desiccation:Effects of Polyethylene Glycol and Abscisic Acid

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American Journal of Plant Sciences 2017年第10期8卷 2501-2515页

作者： Daiane Mugnol Dresch Tathiana Elisa Masetto Tatiane Sanches Jeromini silvana de paula quintao scalon Faculty of Agrarian Sciences Federal University of Grande DouradosDouradosBrazil

The Campomanesia adamantium is a threatened species from Brazil Savannah which seeds are desiccation-sensitive and do not withstand storage. This study aimed to reduce the sensitivity of Campomanesia adamantium seeds to desiccation using polyethylene glycol (PEG) and abscisic acid (ABA). Initially, seeds were subjected to PEG (0, -1.48, and -2.04 MPa) with or without ABA (100 μM) during 120 h, followed fast drying (silica gel) or slow drying (laboratory environment), at 20%, 15%, and 10% moisture content. In the second experiment, the seeds were PEG treated (-1.48 MPa) which provided the best results in the first experiment;the seeds were then subjected to different incubation times in PEG (30, 60, 90, or 120 h) and ABA (0, 10ˉ3, 10ˉ4, and 10ˉ5 μM), following the seeds were fast dried at 15% moisture content. The slow drying should be avoided, even in seeds previously subjected to osmotic conditioning with or without ABA. Seeds submitted to PEG treatment (-1.48 MPa/120h) without ABA and PEG (-1.48 MPa) with 10ˉ3 or 10ˉ4 μM of ABA (90 h), followed by fast drying at 15% moisture content showed reduction of desiccation sensitivity and high germination and vigor when compared to the other treatments.

关键词： Abscisic Acid Osmotic Conditioning Polyethylene Glycol Water Stress

Drying,Storage and Osmotic Conditioning of Psidium guineense Swartz Seeds

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American Journal of Plant Sciences 2014年第17期5卷 2591-2598页

作者： Tathiana Elisa Masetto Eliane Marques da Silva Neves silvana de paula quintao scalon Daiane Mugnol Dresch Federal University of Grande Dourados in Dourados DouradosBrazil

This study aimed to evaluate the tolerance of Psidium guineense Swartz seeds to air-dry storage and priming. desiccation tolerance was analyzed for seed moisture content of 15%, 10% and 5%. The longevity of seeds during storage was analyzed in seeds with 5% and 10% moisture content maintained in cold and dry chamber (16°C ± 1°C/40% RH), laboratory environment (25°C ± 2°C/60% RH), refrigerator (5°C ± 1°C) and freezer (-18°C ± 1°C) during 90 days, and the priming effect in seeds that were imbibed for 5 or 10 days in polyethylene glycol 6000 at osmotic potentials of -0.3 MPa, -0.5 MPa, -0.7 MPa and -1.3 MPa. The quality of seeds was evaluated after desiccation, storage, and priming by the seeds germination, fresh weight of seedlings, seedlings growth, and germination medium time. The experimental design was completely randomized, with four replications of 25 seeds each one. Although a reduction in seed germination and seedling growth was observed in seeds with 10% and 5% moisture content, drying did not cause complete loss of seed germination and seedling development. Sour guava seeds exhibited an orthodox response to desiccation tolerance and storage. Seed germination and seedling growth were reduced with increased osmotic potential of osmoconditioning. However, conditioning of 10 days increased the seeds germination and optimized the average germination time to 20 days. The seeds of Psidium guineense are able to tolerate desiccation and storage for up to 90 days at a temperature of 5°C ± 1°C and priming for 10 days is a promising technique for propagation of P. guineense.

关键词： Myrtaceae Brazilian Savannah Longevity Polyethylene Glycol

Main visual symptoms associated with refractive errors and spectacle need in a Brazilian population

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International Journal of Ophthalmology(English edition) 2016年第11期9卷 1657-1662页

作者： silvana Schellini Fabio Ferraz paula Opromolla Laryssa Oliveira Carlos Padovani Botucatu Medical School University of State of Sao Paulo

AIM： To determine the main visual symptoms in a Brazilian population sample, associated to refractive errors（REs） and spectacle need to suggest priorities in preventive ***： A cross-sectional study was conducted in nine counties of the southeast region of Brazil, using a systematic sampling of households, between March 2004 and July 2005. The population was defined as individuals aged between 1 and 96 y, inhabitants of 3600 residences to be evaluated and 3012 households were included,corresponding to 8010 subjects considered for participation in the survey, of whom 7654 underwent ophthalmic examinations. The individuals were evaluated according their demographic data, eye complaints and eye examination including the RE and the need to prescribe spectacles according to age. Statistical analysis was performed using SPSS software package and descriptive analysis using 95% confidence intervals（P〈0.05）. RESULTS： The main symptom detected was asthenopia, most frequent in the 2ndand 3rddecades of life, with a significant decline after the *** was the RE most associated with asthenopia. Reduced near vision sight was more frequent in those ≥40y with a progressive decline *** were most frequently required in subjects of≥40 years of ***： The main symptom related to the vision was asthenopia and was associated to astigmatism. The greatest need for spectacles prescription occurred after40＇s, mainly to correct near vision. Subjects of ≥40years old were determined to be at high risk of uncorrected REs. These observations can guide intervention programs for the Brazilian population.

关键词： population study visual symptoms refractive error spectacle need

Role of minimally invasive surgery in complex adnexal tumours and ovarian cancer

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World Journal of Obstetrics and Gynecology 2014年第3期3卷 109-117页

作者： Juan Gilabert-Estelles Cristina Aghababyan paula Garcia Jesus Moscardo Susana Royo silvana Aniorte Juan Gilabert-Aguilar Hospital General Universitario de Valencia department of Pediatrics Obstetrics and Gynecology University of Valencia Hospital Universitario Casa de Salud

Ovarian cancer is one of the most common causes of cancerrelated death in women. Adnexal masses are frequently diagnosed during reproductive age and often require surgical removal. The risk of malignancy when dealing with a complex adnexal mass should be defned prior to surgery and several scoring systems may be useful for this purpose. Laparoscopic management of ovarian tumours allows a minimally invasive approach with respect to several oncological assumptions. In the last decade concerns have been raised regarding the risk of cyst rupture and tumour spillage as a con-sequence of the laparoscopic technique itself both in early and advanced stages of ovarian cancer. Although limited data have been reported in the literature on the use of minimally invasive techniques in ovarian cancer, the clear benefits of this approach must be balanced with the potential hazards in different clinical situations. Laparoscopic staging in borderline tumours and presumed early-stage ovarian cancer performed by a laparoscopic oncologist seems to be safe and effec-tive when compared to laparotomy. The precise role of laparoscopy in patients with more advanced cancer is still to be defned, and the risk of suboptimal surgery should never outweigh the potential benefits of mini-mally invasive surgery. Thus, a tailored prediction of optimal laparoscopic debulking is mandatory in these patients.

关键词： Ovarian cancer Laparoscopy Borderline tumour Adnexal masses

Sugarcane Transcript Profiling Assessed by cDNA-AFLP Analysis during the Interaction with Sugarcane Mosaic Virus

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Advances in Microbiology 2014年第9期4卷 511-520页

作者： Cibele N.F.Medeiros Marcos C.Goncalves Ricardo Harakava silvana Creste paula M.Nóbile Luciana R.Pinto Dilermando Perecin Marcos G.A.Landell Sugarcane Research Center Instituto Agronomico de Campinas(IAC)Ribeirao PretoBrazil Faculdade de Ciencias Agrárias e Veterinárias Universida de Estadual PaulistaJaboticabalBrazil Plant Virology Laboratory CPDSVInstituto BiológicoSao PauloBrazil Plant Pathology Biochemistry Laboratory CPDSVInstituto BiológicoSao PauloBrazil

Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanically inoculated under greenhouse controlled conditions and sampled at 24, 48 and 72 hours after inoculation. A total of 392 transcript-derived fragments (TDFs) were verified in the resistant variety against 380 in the susceptible one. The two sugarcane genotypes showed differential behavior in the number of induced and repressed TDFs along the time-course samplings. Ten out of 23 sequenced TDFs (unique from the resistance variety), showed identity with known plant sequences, mostly related to plant defense mechanisms against pathogens. The cDNA-AFLP technique was effective in revealing changes in the transcription profile within and between contrasting varieties when challenged by SCMV.

关键词： Plant Resistance Plant Viruses Molecular Markers Candidate Genes cDNA-AFLP SCMV

dengue in renal transplant recipients: Clinical course and impact on renal function

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World Journal of Transplantation 2017年第1期7卷 57-63页

作者： paula Frassinetti Castelo Branco Camur?a Fernandes Reed AndréSiqueira Evelyne Santana Gir?o Rainne AndréSiqueira Márcia Uchoa Mota Leyla Castelo Branco Fernandes Marques silvana Cristina Albuquerque Andrade Wilson Mendes Barroso S?nia Leite Silva Bruno Gomes Rodrigues dos Santos Claúdia Maria Costa de Oliveira Kidney Transplant Unit Hospital Universitário Walter Cantídio Universidade Federal do Ceará department of Nephrology of Universidade Estadual do Ceará Universidade Estadual do Ceará

AIM To present clinical characteristics from renal transplant recipients with dengue fever and its impact on graft *** We retrospectively evaluated 11 renal transplant recipients(RTR) with dengue infection confirmed by laboratory test, between January 2007 and July 2012, transplanted in the Renal Transplant Center of Walter Cantídio University Hospital from Federal University of Ceará. RESULTS Positive dengue serology(IgM) was found in all patients. The mean time between transplant and dengue infection was 43 mo. Fever was presented in all patients. Nine patients presented with classical dengue and two(18%) with dengue hemorrhagic fever. All cases had satisfactory evolution with complete recovery of the symptoms. The time for symptom resolution varied from 2 to 20 d, with an average of 9 d. An increase of creatinine after the infection was observed in three(27.2%) patients with no clinically impact on the kidney graft function. CONCLUSION RTR with dengue infection seems to have a clinical presentation and evolution similar to those seen in the general population, with no long-term damage to patient and to the graft.

关键词： Kidney Renal Transplant dengue Clinical Brazil