检索结果-南通市图书馆

Genetic ancestry analysis in non-alcoholic fatty liver disease patients from Brazil and Portugal

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Hepatology 2015年第10期7卷 1433-1438页

作者： Lourianne Nascimento Cavalcante Jose Tadeu Stefano Mariana V Machado Daniel F Mazo Fabiola Rabelo Kiyoko Abe Sandes Flair Jose Carrilho Helena Cortez-Pinto Andre castro Lyra Claudia P de oliveira Gastro-Hepatology department Hospital Sao Rafael Division of Gastroenterology and Hepatology Department of GastroenterologyUniversity of Sao Paulo School of Medicine department of Gastroenterology Laboratory of NutritionUniversity Hospital of Santa Maria University Life Sciences department University of Bahia State Faculty of Medicine Federal University of Bahia

AIM:To study the association between genetic ancestry,non-alcoholic fatty liver disease(NAFLD) metabolic characteristics in two cohorts of patients,from Brazil and Portugal. METHODS:We included 131 subjects from Brazil [(n = 45 with simple steatosis(S. Steatosis) and n = 86 with nonalcoholic steatohepatitis(NASH)] and 90 patients from Portugal(n = 66,S. Steatosis; n = 24,NASH). All patients had biopsy-proven NAFLD. In histologic evaluation NAFLD activity score was used to assess histology and more than 5 points defined NASH in this study. Patients were divided into two groups according to histology diagnosis:simple steatosis or non-alcoholic statohepatitis. Genetic ancestry was assessed using real-time polymerase chain reaction. Seven ancestry informative markers(AT3-I/D,LPL,Sb19.3,APO,FYNull,PV92,and CKMM) with the greatest ethnicgeographical differential frequencies(≥ 48%) were used to define genetic ancestry. Data were analyzed using R PROJECTS software. Ancestry allele frequencies between groups were analyzed by GENEPOP onlineand the estimation of genetic ancestry contribution was evaluated by ADMIX-95 software. The 5% alpha-error was considered as significant(P derly patients with diabetes(NASH 56 ± 1.1 years old vs S. Steatosis 51 ± 1.5 years old,P = 3.7 x 10-9),dyslipidemia(NASH 63% vs S. Steatosis 37%,P = 0.009),higher fasting glucose levels(NASH 124 ± 5.2 vs S. Steatosis 106 ± 5.3,P = 0.001) and Homeostatic Model of Assessment index > 2.5 [NASH 5.3(70.8%) vs S. Steatosis 4.6(29.2%) P = 0.04]. In the Portuguese study population,dyslipidemia was present in all patients with NASH(P = 0.03) and hypertension was present in a larger percentage of subjects in the S. Steatosis group(P = 0.003,respectively). The genetic ancestry contribution among Brazilian and Portuguese individuals with NASH was similar to those with S. Steatosis from each cohort(Brazilian cohort:P = 0.75; Po

关键词： Ancestry Nonalcoholic fatty liver disease Simple steatosis Nonalcoholic steatohepatitis Admixed population

Essential role of small bowel capsule endoscopy in reclassification of colonic inflammatory bowel disease type unclassified

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Endoscopy 2017年第1期9卷 34-40页

作者： Sara Monteiro Francisca Dias de castro Pedro Boal Carvalho Bruno Rosa Maria Joao Moreira Rolando Pinho Miguel Mascarenhas Saraiva José Cotter department of Gastroenterology Hospital da Senhora da Oliveira-Guimaraes ManopH-Endo-scopy and Digestive Motility Laboratory Portugal and CUF Institute School of Medicine University of Minho ICVS/3B’s PT Government Associate Laboratory

AIM To evaluate the role of small bowel capsule endoscopy(SBCE) on the reclassification of colonic inflammatory bowel disease type unclassified(IBDU).METHODS We performed a multicenter, retrospective study including patients with IBDU undergoing SBCE, between 2002 and 2014. SBCE studies were reviewed and the inflammatory activity was evaluated by determining the Lewis score(LS). Inflammatory activity was considered significant and consistent with Crohn's disease(CD) when the LS ≥ 135. The definitive diagnosis during follow-up(minimum 12 mo following SBCE) was based on the combination of clinical, analytical, imaging, endoscopic and histological *** Thirty-six patients were included, 21 females(58%) with mean age at diagnosis of 33 ± 13(15-64) years. The mean follow-up time after the SBCE was 52 ± 41(12-156) mo. The SBCE revealed findings consistent with significant inflammatory activity in the small bowel(LS ≥ 135) in 9 patients(25%); in all of them the diagnosis of CD was confirmed during follow-up. In 27 patients(75%), the SBCE revealed no significant inflammatory activity(LS < 135); among these patients, the diagnosis of Ulcerative Colitis(UC) was established in 16 cases(59.3%), CD in 1 case(3.7%) and 10 patients(37%) maintained a diagnosis of IBDU during follow-up. A LS ≥ 135 at SBCE had a sensitivity = 90%, specificity = 100%, positive predictive value = 100% and negative predictive value = 94% for the diagnosis of *** SBCE proved to be fundamental in the reclassification of patients with IBDU. Absence of significant inflammatory activity in the small intestine allowed exclusion of CD in 94% of cases.

关键词： Inflammatory bowel disease Inflammatory bowel disease type unclassified Capsule endoscopy Crohn’s disease Lewis score Reclassification

Biquinho pepper(Capsium chinense):Bioactive compounds,in vivo and in vitro antioxidant capacities and anti-cholesterol oxidation kinetics in fish balls during frozen storage

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Bioscience 2022年第3期47卷 512-523页

作者： Bárbara Jardim Mariano Vanessa Sales de oliveira Davy William Hidalgo Chávez Rosane Nora castro Cristiano Jorge Riger Jéssica Sodré Mendes Marcelo da Costa Souza Alexandra Christine Helena Frankland Sawaya Geni Rodrigues Sampaio Elizabeth Aparecida Ferraz da Silva Torres Tatiana Saldanha department of Food Technology Institute of TechnologyUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil department of Chemistry Institute of ChemistryUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil department of Biochemistry Institute of ChemistryUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil department of Botany Institute of Biological Sciences and HealthUniversity Federal Rural of Rio de JaneiroRodovia Br 465SeropédicaRJ23890-000Brazil Faculty of Pharmaceutical Science University of CampinasUNICAMPCampinasSPBrazil department of Nutrition School of Public HealthUniversity of São Paulo (USP)Ave.Dr.Arnaldo715São PauloSP01246-904Brazil

The present study reports bioactive constituents from biquinho pepper,such as carotenoids,capsaicinoids,and capsinoids,identified by *** pepper extract showed an antioxidant potential by DPPH(33.17±0.08%),ORAC(32.35±0.02μM TE/g),β-carotene/linoleic acid(55.80±1.30%),and FRAP(315.88±4.23μmol TE/g)***,in vivo antioxidant analyses demonstrated that the pepper extract(170μg/mL)decreased the damage promoted by H_(2)O_(2) in Saccharomyces cerevisiae *** balls were prepared using different concentrations of pepper(0.0%,0.5%,0.75%,1.0%)to investigate its antioxidant effect during frozen *** pepper effectively reduced the formation of cholesterol oxides and preserved essential fatty acids,mainly in samples containing 1.0%of ***,this treatment was more efficient than sodium erythorbate(pdetermined,showing that both cholesterol degradation and cholesterol oxides formation fit a first-order kinetic model(R^(2)>0.8).

关键词： Bioactive compounds Capsicum chinense Cholesterol oxides Frozen storage Natural antioxidants Unsaturated lipids

Risk Analysis of the Economic Benefits of Ochroma pyramidale:A Case Study of Forest Planting in Brazil

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Agricultural Science and Technology(B) 2018年第7期8卷 444-453页

作者： Ricardo Hideaki Miyajima Vitória castro Santos Barreto Paulo Andréde oliveira Gislaine Cristina Batistela Danilo Simoes department of Forestry Science Sao Paulo State University(Unesp)School of AgricultureBotucatuSao Paulo 18610-307Brazil department of Agribusiness State Center for Technological Education Paula SouzaBotucatuSao Paulo 18606-855Brazil department of Production Engineering Sao Paulo State University(Unesp)Campus of ItapevaSao Paulo 18409-010Brazil

The forest investment projects,analyzed through the application of simulation techniques,allow measuring more accurately the benefits or losses,considering the systemic uncertainties related to forest *** this perspective,the objective of this study was to identify the relative importance of sources of uncertainties in determining the net present value(NPV)of planting of Ochroma pyramidale on a forested property located in the state of S?o Paulo,***,it assigns probability distributions to the parameters with uncertainties and applies the Monte Carlo technique to obtain the stochastic behavior of the NPV,of the modified internal rate of return(MIRR)and the profitability index(PI).The probabilistic results of the quantitative methods analyzed allowed us to conclude that for the planting ***,with 5-year cutting age,indicated economic *** addition,there is a probability that 84.6%of the project will add economic value to the invested capital.

关键词： Balsa wood forest plantation forest management net present value Monte Carlo

Isolated Soy Protein-Based Diet Ameliorates Glycemia and Antioxidants Enzyme Activities in Streptozotocin-Induced Diabetes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2014年第21期5卷 2089-2096页

作者： Roberta Hack Mendes Martine Kienzle Hagen Jaqueline Barp Erna Vogt de Jong Jú lia Dubois Moreira lvaro Reischak-oliveira Maria Clá udia Irigoyen Adriane Belló -Klein Physiology departament Federal University of Rio Grande do Sul Porto Alegre Brazil Institute of Nutrition Josué de castro Rio de Janeiro Federal University Rio de Janeiro Brazil department of Internal Medicine Federal University of Rio Grande do Sul Porto Alegre Brazil Institute of Food Science and Technology Federal University of Rio Grande do Sul Porto Alegre Brazil department of Nutrition Health Sciences Centre Federal University of Santa Catarina Florian&#243 polis Brazil School of Physical Education Federal University of Rio Grande do Sul Porto Alegre Brazil Hypertension Unit Heart Institute Federal University of S&#227 o Paulo S&#227 o Paulo Brazil

The objective of this study was to evaluate the changes induced by isolated soy protein (ISP)-based diet on glycemia and oxidative stress biomarkers in diabetic rats. Fifteen male Wistar rats (35 ± 4 g, aged 21 days) were assigned to three groups: Casein (C group), which received casein-based diet during experimental protocol;Diabetic treated with Casein (D + C group) that received casein-based diet before and after diabetes induction;Diabetic treated with ISP (D + S group) that received casein-based diet before diabetes induction and after received ISP-based diet for the experimental protocol. Diabetes was induced by a single dose of streptozotocin (50 mg/kg body weight i.v.). After three weeks of dietary treatment, total nitrates, lipid peroxidation, antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) were measured in heart homogenates. ISP-based diet promoted an improvement in the glycemic levels of diabetic rats compared with casein-based diet (362 ± 25 vs 461 ± 30 mg/dL). CAT activity demonstrated a significant decrease in D + C and D + S groups. D + S group presented a significant increase in SOD and GST activities. Lipid peroxidation was not different among experimental groups. The overall results suggested the potential benefits of ISP-based diet consumption to improve the life quality of diabetic patients.

关键词： Isolated Soy Protein Diabetes Oxidative Stress

SARS-CoV-2 productively infects primary human immune system cells in vitro and in CoviD-19 patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2022年第4期14卷 51-64页

作者： Marjorie C.Pontelli italo A.castro Ronaldo B.Martins Leonardo La Serra Flavio P.Veras Daniele C.Nascimento Camila M.Silva Ricardo S.Cardoso Roberta Rosales Rogério Gomes Thais M.Lima Juliano P.Souza Brenda C.Vitti Diego B.Caetite Mikhael H.F.de Lima Spencer D.Stumpf Cassandra E.Thompson Louis-Marie Bloyet Juliana E Toller-Kawahisa Marcela C.Giannini Leticia P.Bonjorno Marial.F.Lopes Sabrina S.Batah Li Siyuan Rodrigo Luppino-Assad Sergio C.L.Almeida Fabiola R.oliveira Maira N.Benatti Lorena L.F.Pontes Rodrigo C.Santana Fernando C.Vilar Maria Auxiliadora-Martins Pei-Yong Shi Thiago M.Cunha Rodrigo T.Calado JoséC.Alves-Filho Dario S.Zamboni Alexandre T.Fabro Paulo Louzada-Junior Rene D.R.oliveira Sean P.J.Whelan Fernando Q.Cunha Eurico Arruda Virology Research Center Ribeirao Preto Medical SchoolUniversity of Sao Pauloibeirao PretoSao Paulo 14049-900.Brazil Center of Research in Inflammatory Diseases Ribeirao Preto Medical SchoolUniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil department of Celland Molecular Biology Ribeirao Preto Medical SchoolUniversityof Sao PauloRibeirao PretoSao Paulo 14049-900 Brazil Regional Blood Centerof Ribeirao Preto Ribeirao Preto Medical ScholUniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil department of Biochemistry&Molecular Biology the University of Texas Medical BranchGalvestonTX7755USA Divisions of Clinical lmmunology Infectious Diseases and Intensive Care UnitRibeirao Preto Medical Schooniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil department of Pathology Ribeirao Preto Medical Scholniversity of Sao PauloRibeirao PretoSao Paulo 14049-900Brazil department of Molecular Microbiology.Washington University in St.Louis Saint LouisMO 63110USA

The severe acute respiratory syndrome coronavirus 2(SARS-CovV-2)infection is associated with a hyperinflammatory state and lymphocytopenia,a hallmark that appears as both signature and prognosis of disease severity *** cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion,it is still unclear whether direct SARS-Cov-2 infection of immune cells could also play a role in this scenario by harboring viral *** found that monocytes,as well as both B and T lymphocytes,were susceptible to SARS-Cov-2 infection in vitro,accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell *** addition,flow cytometry and immunofluorescence analysis revealed that SARS-Cov-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019(CoVID-19)*** rates of SARS-Cov-2-infected monocytes in peripheral blood mononuclear cells from CoVID-19 patients increased over time from symptom onset,with SARS-CoV-2-positive monocytes,B cells,and CD4+T lymphocytes also detected in postmortem lung *** results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in covID-19 patients might have important implications for disease pathogenesis and progression,immune dysfunction,and virus spread within the host.

关键词： SARS-CoV-2 CoVID-19 lymphocytopenia peripheral blood mononuclear cell(PBMC) lymphocytes monocytes apoptosis

Risk factors for osteoporosis in inflammatory bowel disease patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Pathophysiology 2015年第4期6卷 210-218页

作者： Carla Andrade Lima Andre castro Lyra Raquel Rocha Genoile oliveira Santana Pos-graduation program in medicine and health Federal University of Bahia department of Gastroenterology Federal University of Bahia department of Sciences of Nutrition School of NutritionFederal University of Bahia

Inflammatory bowel disease(IBD) patients exhibit higher risk for bone loss than the general population. The chronic inflammation causes a reduction in bone mineral density(BMD), which leads to osteopenia and osteoporosis. This article reviewed each risk factor for osteoporosis in IBD patients. Inflammation is one of the factors that contribute to osteoporosis in IBD patients, and the main system that is involved in bone loss is likely RANK/RANKL/osteoprotegerin. Smoking is a risk factor for bone loss and fractures, and many mechanisms have been proposed to explain this loss. Body composition also interferes in bone metabolism and increasing muscle mass may positively affect BMD. IBD patients frequently use corticosteroids, which stimulates osteoclastogenesis. IBD patients are also associated with vitamin D deficiency, which contributes to bone loss. However, infliximab therapy is associated with improvements in bone metabolism, but it is not clear whether the effects are because of inflammation improvement or infliximab use. Ulcerative colitis patients with proctocolectomy and ileal pouches and Crohn's disease patients with ostomy are also at risk for bone loss, and these patients should be closely monitored.

关键词： Bone mineral density Crohn’s disease Osteoporosis

Larvicidal Evaluation of Aedes aegypti and Antioxidant,Cytotoxic and Antimicrobial Potential of the Aqueous Acid Extract of Pseudoxandra cuspidata(ANNOACEAE).

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmacy and Pharmacology 2021年第2期9卷 53-61页

作者： Ranggel Carvalho Simões Rosany Lopes Martins Erica de Menezes Rabelo Alex Bruno Lobato Rodrigues Ana Luzia Ferreira Farias Patrick de castro Cantuária Marlon de oliveira do Nascimento Sheylla Susan Moreira da Silva de Almeida Laboratory of Pharmacognosy and Phytochemistry of the Federal University of Amapá.Rodovia Juscelino Kubistchek KM-02.Jardim Marco Zero-Macapá-APBrazil Plant Taxonomy Laboratory Institute of Scientific and Technological Research of the State of AmapáHighway Jucelino KubistichekKm 1068903419MacapáBrazil

The Anonnaceae family has 2,500 species and 135 genera, which has about 14 representatives of species in Brazil,including Pseudoxandra cuspidata. The objective of this research was to study the aqueous-acid extract of P. cuspidata, evaluatingits antioxidant, larvicidal, antimicrobial and cytotoxic potential. In the preliminary phytochemical analysis, there were positivity toorganicacids and alkaloids. Inhibitory Activity of EAA was 65% against DPPH. The AAE presented to A. Salina nauplii at LC50%of 600.79 μg/mL for a periodof 24 hours. In relation to larvicidal activity to Aedes aegypti intermediates, AAE presented the LC50%in 24 Hours of 475.91 ppm and 290.73 ppm in 48 Hours. Regarding the antimicrobial action, the EAA presented 50 mg/mL MIC forP. Aeruginosa and MBC at 100 mg/mL, and for S. aureus it presented MIC of 50 mg/mL and did not present MBC. This researchcharacterized the presence of alkaloids and organicacids present in the aqueousacid extract of P. cuspidata. The extract presentedlowtoxicity in relation to the microcrustacean A. salina. It also had a relevant antioxidant potential (65% of Inhibition),larvicidalaction considered effective, antimicrobialaction for both P. Aeruginosa and S. Aureus even did not present bactericidalaction.

关键词： Pseudoxandra DPPH Artemia salina Aedes aegypti.

Total Phenolic Compounds and Antioxidant Activity of Organic Vegetables Consumed in Brazil

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2015年第9期6卷 798-804页

作者： Verona Borges Ferreira Thadia Turon Costa da Silva Silvia Regina Magalhaes Couto Armando Ubirajara oliveira Sabaa Srur Centro de Ciencias da Saude Instituto de Nutricao Josue de CastroUniversidade Federal do Rio de JaneiroRio de JaneiroBrasil

Organic olericulture differs from conventional agriculture due to the absence of pesticide, synthetic fertilizers, genetically modified organisms, some food additives and ionizing radiation, producing vegetables with a different chemical composition and with different amounts of bioactive compounds. The objective of this study was to quantify, in organic vegetables consumed in Brazil, the amount of total phenolic compounds using Folin-Ciocalteu reagent and the antioxidant activity through DPPH method. Among the analyzed vegetables, the effectiveness in the antioxidant capacity was obtained in decreasing order, by the broccoli (69.35), kale (63.20), cabbage (35.72) and carrot (20.39), expressed in % of DPPH consumption after 30 minutes of reaction. The amount of total phenolic compounds (mg GAE/ml of fluid extract-FE) present in the kale (0.606), broccoli (0.511), cabbage (0.214) and carrots (0.124) demonstrates a positive correlation between phenolic amounts and the antioxidant activity of the vegetables analyzed.

关键词： Organic Food Bioactive Compounds Vegetables Antioxidant DPPH