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Leptin Signaling Modulates Expression of Polycomb and Trithorax Complexes in the brain of Fat Tissue Implanted Polycystic Ovarian Sindrome Mice

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American Journal of Molecular biology 2014年第4期4卷 177-192页

作者： Eduardo Henrique da Silva Freitas Samuel Marcos Ribeiro de Noronha Maria Nazareth Gamboa Ritto Carlos Fernandes baptista Ismael Dale Cotrim Guerreiro da Silva silvana Aparecida Alves Correa-Noronha Ivaldo da Silva Gynecology Department Federal University of Sao Paulo Paulista School of Medicine (UNIFESP-EPM) Sao Paulo Brazil General Medicine Department Federal University of Rio de Janeiro State (UNIRIO) Rio de Janeiro Brazil Translacional Surgery Division Surgery Department Federal University of Sao Paulo Paulista School of Medicine (UNIFESP-EPM) Sao Paulo Brazil General and Specialized Surgery Department Federal University of Rio de Janeiro State (UNIRIO) Rio de Janeiro Brazil

The Polycystic Ovary Syndrome (PCOS) is the most common androgenic disorder in women during reproductive life. PCOS may also be accompanied by metabolic syndrome and recent studies point to leptin as playing a role in disrupting infertility and in changing the energy balance in obese mice through its action on the hypothalamus. The aim is to assess the expression of the Polycomb & Trithorax Complexes genes in brain of mice transplanted with fat tissue from normal mice, in order to better understand the neuronal mechanisms underlying the reversion of PCOS. Three b6 V-Lepob/J mouse groups: Normal weight, obese and seven-day-treatment obese had their brain RNA extracted and submitted to an 84 Polycomb & Trithorax Complexes genes PCR Array plate and MetacoreTM pathways localization. Genomic profiles obtained were compared to the ones of the normal-weight-mice group. Differentially expressed genes were 13% and 26% respectively to control and treatment. Major changes were in genes: Snai1/31;Smarca1/?17;Dnmt3b/4.7;Ezh1/ 15. Altered genes were associated to canonical pathways and provided 3 networks related to epigenetics. Underlying neuronal changes caused by leptin in obese mice brain, there is an important role being played by the histone code. Here there is evidence that leptin drives the chromatin packing to a more condensed pattern. Upregulation of methyltransferase genes, like Ezh1, favors this thought. In summary the Polycomb & Trithorax complexes might answer for the silencing of some downregulated genes in the obese mice brain when exposed to leptin.

关键词： Polycistic Ovarian Sindrome Obesity Polycomb and Trithorax Complexes Leptin Fat Mouse

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Assessment of the Effects of Manual Chest Compression Technique on Atelectasis in Infants: A Randomized Clinical Trial

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International Journal of Clinical Medicine 2014年第9期5卷 507-513页

作者： Natania Florentino Diniz1] Evelim Leal de Freitas Dantas Gomes1] Cristiane Aparecida Moran1] silvana Alves Pereira1] Livia Maria de Andrade Martins1] Luciana Carnevalli Pereira1] Universidade Nove de Julho(UNINOVE) Sao PauloBrazil 2Universidade Federal do Rio Grande do Norte(UFRN/FACISA)Santa CruzBrazil

Objectives: To evaluate the effect of manual chest compression (MCC) in the variables of oxygenation, hemodynamic and respiratory effeteness in infants suffering from respiratory diseases with atelectasis. Methods: Controlled clinical trial, in which 38 infants were evaluated,19 ineach study group (group A: atelectasis and group b: control). Data were measured before, immediately after and 10 minutes after the end of the technique’s application. Results: The average age was of 5.05 months. There was an increase of RR in group A immediately after the application of the technique and signs of respiratory distress with a decrease in the oxygen saturation. Conclusion: There was a reduction in SpO2, an increase of RR and a worsening of clinical signs of respiratory distress. Given this, one may consider that there is a controversy about the benefits, mechanism of action, physiological and therapeutic effects of MCC when applied to infants.

关键词： Pulmonary Atelectasis Physiotherapy Modality Respiratory Therapy Infant Infant Care Clinical Trials

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A Comprehensive bioinformatics Analysis of the Lipoxygenases Superfamily in Shewanella Woodyi Strain （Strain ATCC 519081MS32）

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材料科学与工程（中英文b版） 2014年第2期4卷 34-41页

作者： silvana Pompeia Val-Moraes Eliana Gertrudes de Macedo Lemos University of Estadual Paulista UNESP/FCAV Department Technology LBMP Via de Acesso Prof. Paulo Donato Castellane s/n.Jabotieabal Sao Paulo Brazil

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Leptin Causes the Early Inhibition of Glycolysis-and TCA Cycle-Related Genes in the brain of Ob/Ob Mice to Restore Fertility

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American Journal of Molecular biology 2014年第3期4卷 105-113页

作者： Carlos Fernandes baptista Samuel Marcos Ribeiro de Noronha Maria de Nazareth Gamboa Ritto Eduardo Henrique da Silva Freitas Melquíades Pereira Júnior Mauro Abi Haidar Ismael Dale Cotrim Guerreiro da Silva silvana Aparecida Alves Corrêa de Noronha Marisa Teresinha Patriarca Gynecology Department Escola Paulista de Medicina/Universidade Federal de Sao Paulo(EPM-UNIFESP)São PauloBrazil Departmento de Cirurgia Geral e Especializada Universidade Federal do Rio de Janeiro(UNIRIO)Rio de JaneiroBrazil Surgery Department Escola Paulista de Medicina/Universidade Federal de Sao Paulo(EPM-UNIFESP)Sao PauloBrazil Departmento de Medicina Geral Universidade Federal do Rio de Janeiro(UNIRIO)Rio de JaneiroBrazil

Introduction: Polycystic ovarian syndrome (PCOS) is undoubtedly the commonest androgen disorder in woman’s fertile period and certainly one of the most prevalent causes of anovulation. The syndrome has an estimated prevalence of 4% - 10% among women of childbearing age. Previously, our group demonstrated the effect of gonadal white adipose tissue transplantation from wild-type lean and fertile female mice to isogenic obese anovulatory ob/ob mice. These complex metabolic interrelationships between obesity and PCOS have yet to be fully understood. The aim of this study was to evaluate the effect of gonadal white adipose tissue (WAT) transplantation from the wild-type lean and fertile female mice to isogenic obese, anovulatory mice (Lep ob/Lep ob) on the expression of glycolysis- and TCA cycle-related genes and obtain a general view of the glucose metabolism in the brain of these animals. Methods: Fifteen ob/ob mice ranging from 2 to 3 months of age were divided into 3 experimental groups: control normal weight (n = 5), obese control (n = 5) and obese 7 days leptin treated (n = 5). The whole brains of the mice were processed for RNA extraction. The samples from each group were used to perform PCR assays using an array plate containing 84 primers to study the glucose metabolism-related genes. Results: The glycolysis- and TCA cycle-related genes were significantly downregulated. The most significantly affected genes were as follows: for glycolysis (fold regulation with p bpgm,Aldob, andEno3 (119, 45, 18, and 28 times less, respectively);and for the TCA cycle (fold regulation with p b, andMdh2 (84, 27, and 37 times less, respectively).Conclusion: The seven-day leptin treated mice show a decrease in the glucose metabolism. These results confirm the ability of the adipose tissue-derived hormone leptin to regulate early crucial genes that are related to glycolysis mechanisms and to the TCA cycle. This hormone seems to revert early the central phys

关键词： PCOS Obesity Leptin Glycolysis TCA Cycle Gene Expression

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In Silico Prediction Structure and Function for Novel Protein Alkane Hydroxylase the Pseudomonas Nitroreducens

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材料科学与工程（中英文A版） 2014年第7期4卷 241-248页

作者： silvana Pomp6ia Val-Moraes Gisele Regina Rodrigues Douglas Antonio Alvaredo Paixào, Eliana Gertrudes Macedo Lemos Department Technology LBMP Via de Acesso Prof. Paulo Donato Castellane s/n Univ. Estadual Paulista UNESP/FCAVJabotieabal S6o Paulo Brazil Laborat6rio Nacional de Ciência e Tecnologia do bioetanol (CTbE) Rua Giuseppe Màximo Scalfaro Sao Paulo Brazil

Currently in brazil is used the b5 fuel composed of 95% of diesel oil and 5% biodiesel. It has a mixture of alkanes, alkenes and aromatic compounds. The high sulfur content in the fuel determines brazilian great cost to the public health system. There is a 1.5% increase in hospitalizations for ischemic disease heart in the elderly and more than 4% for lung diseases in children and the elderly. Polycyclic aromatic hydrocarbons derived refined petroleum products are ubiquitous environmental due contamination of soil arising from spills or leaks most inhibit factors to soil fertility, crop productivity may bio-accumulate in food chains thus causing carcinogenesis of some organs and mutagenesis in the genetic material. In this work, analyzed the novel alkane hydroxylase described for Pseudomonas nitroreducens registered in Genebank as alkb gene (KJ023684.1). These analyses identified the protein described as alkane hydroxylase, superfamily and function, secondary structures, the phosphorylation sites, the protein GRAVY (grand average of hydropathy) and protein isoelectric point. The binding site is di-iron. This suggested that novel alkane hydroxylase deserve special attention to continue our work in situ, will be enzyme characterization and biosurfactants production or bioremediation have potential uses in environmental monitoring.

关键词：假单胞菌二级结构羟化酶蛋白质烷烃公共卫生体系建设生物表面活性剂预测

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