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CEUS and Fibroscan in non-alcoholic fatty liver disease and non-alcoholic steatohepatitis

World Journal of Hepatology 2014年第7期6卷 496-503页

作者： sila cocciolillo Giustino Parruti Leonardo Marzio Digestive Physiopathology Unit Gabriele d’Annunzio University Pescara Civic Hospital 65124 Pescara Italy Infectious and Tropical Diseases Unit Pescara Civic Hospital 65124 Pescara Italy

AIM: To determine intra-hepatic blood flow and liver stiffness in patients with non-alcoholic fatty liver disease(NAFLD) and non-alcoholic steatohepatitis (NASH) using contrast-enhanced ultrasound and ***: This prospective study included 15 patients with NAFLD, 17 patients with NASH and 16 healthy *** each patient, real-time ultrasound was used to locate the portal vein (PV) and the right liver lobe, and 5 mL of SonoVue? was then injected intravenous in a peripheral vein of the left arm over a 4-s span. Digital recording was performed for 3 min thereafter. The recording was subsequently retrieved to identify an area of interest in the PV area and in the right liver parenchyma(LP) to assess the blood flow by processing the data using dedicated software (Qontrast?, Bracco, Italy).The following parameters were evaluated: percentage of maximal contrast activity (Peak%), time to peak (TTP, s), regional blood volume (RBV, cm3), regional blood flow (RBF, cm3/s) and mean transit time (MTT, s).At 24-48 h post-injection, liver stiffness was evaluated using Fibroscan and measured in kPa. The statistical evaluation was performed using Student’s t ***: In the PV, the Peak%, RBV and RBF were significantly reduced in the NAFLD and NASH patientscompared with the controls (Peak%: NAFLD 26.3 ± 6.6,NASH 28.1 ± 7.3 vs controls 55.8 ± 9.9, P < 0.001;RBV: NAFLD 4202.3 ± 3519.7, NASH 3929.8 ± 1941.3vs controls 7473 ± 3281, P < 0.01; RBF: NAFLD 32.5± 10.8, NASH 32.7 ± 12.1 vs controls 73.1 ± 13.9, P< 0.001). The TTP in the PV was longer in both patient groups but reached statistical significance only in the NASH patients compared with the controls (NASH 79.5± 37.8 vs controls 43.2 ± 30, P < 0.01). In the LP,the Peak%, RBV and RBF were significantly reduced in the NAFLD and NASH patients compared with the controls (Peak%: NAFLD 43.2 ± 7.3, NASH 41.7 ± 7.7 vs controls 56.6 ± 6.3, P < 0.001; RBV: NAFLD 4851.5± 2009, NASH 5069.4 ± 2292.5 vs controls 6922.9 ±2461.

关键词： Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis Contrast-enhanced ultrasound Fibroscan Hepatic blood flow Liver stiffness

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Endoscopic fluorescent lymphography for gastric cancer

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World Journal of Gastrointestinal Endoscopy 2023年第2期15卷 32-43页

作者： Calcedonio Calcara sila cocciolillo Ylenia Marten Canavesio Vincenzo Adamo Silvia Carenzi Daria Ilenia Lucci Alberto Premoli Digestive Endoscopy Unit S.Andrea HospitalVercelli 13100Italy Digestive Endoscopy Unit Padre Pio HospitalVasto 66054Italy Postgraduate School of Gastroenterology Genova UniversityGenova 16132Italy General Surgery Unit S.Andrea HospitalVercelli 13100Italy

Lymphography by radioisotope or dye is a well-known technique for visualizing the lymphatic drainage pattern in a neoplastic lesion and it is in use in gastric *** green(ICG)more recently has been validated in fluorescent lymphography studies and is under evaluation as a novel tracer agent in gastric *** amount and dilution of ICG injected as well as the site and the time of the injection are not *** our unit,endoscopic submucosal injections of ICG are made as 0.5 mg in 0.5 mL at four peritumoral sites the day before surgery(for a total of 2.0 mg in 2.0 mL).Detection instruments for ICG fluorescence are ***-infrared systems integrated into laparoscopic or robotic instruments(near-infrared fluorescence imaging)have shown the most promising *** fluorescence recognizes the node that receives lymphatic flow directly from a primary *** is defined as the sentinel lymph node,and it has a high predictive negative value at the cT1 stage,able to reduce the extent of gastrectomy and lymph node *** also enhances the number of lymph nodes detected during extended lymphadenectomy for advanced gastric ***,the practical effects of ICG use in a single patient are not yet *** of the technique and further studies are needed before fluorescent lymphography can be used extensively *** then,current guidelines recommend an extensive lymphadenectomy as the standard approach for gastric cancer with suspected metastasis.

关键词： Indocyanine green Fluorescence Lymphography Sentinel lymph node Gastric cancer Lymphadenectomy

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Nanosized GdVO_4 powders synthesized by sol-gel method using different carboxylic acids

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Rare Metals 2018年第7期37卷 561-567页

作者： Sulawan Kaowphong Nawapong Chumha Piyarat Nimmanpipug sila Kittiwachana Department of Chemistry Faculty of Science Chiang Mai University

Nanosized GdVO4 powders were synthesized via a sol-gel method using different carboxylic acids as chelating agent, followed by calcination at 600 ℃for 3 h. The effect of different carboxylic acids such as citric acid, malic acid, and tartaric acid on the characteristics of the nanosized GdVO4 powders was investigated. The GdVO4 powder was also synthesized without carboxylic acid for comparison. The thermal decomposition process of the car- boxylate precursors was investigated by thermogravimetric differential thermal analysis （TG-DTA）. X-ray diffraction （XRD）, Fourier transform infrared spectroscopy （FTIR）, field emission scanning electron microscope （FESEM）, transmission electron microscope （TEM）, and surface area measurement data were used to confirm the formation of nanocrystalline GdVO4 powders. It is found that the synthesis using the carboxylic acid with higher heat of combustion results in the powder with larger crystallite size. The difference in the steric effect of the acids used, which was evaluated by a computational method, also affects the degree of agglomeration of the synthesized powders.

关键词： GdV04 Electron microscopy Sol-gel processes X-ray method

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In vitro transdifferentiation of corneal epithelial-like cells from human skin-derived precursor cells

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International Journal of Ophthalmology(English edition) 2012年第2期5卷 158-163页

作者： Sarawut Saichanma Ahnond Bunyaratvej Monnipha sila-asna Department of Pathology Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok 14140 Thailand Cell Engineering and Tissue Growth Institute of Molecular Biosciences Mahidol University Phuttamonthon Salaya Nakornpathom 73170 Thailand PYY Foundation (Foundation for Sustainable Development) PO Box 19 (kratum ban) Samutakorn 74110 Thailand

The damage of human corneal cells encounter with the problem of availability of corneal cells for replacement. Limitation of the source of corneal cells has been realized. An attempt of development of corneal epithelial-like cells from the human skin-derived precursor (hSKPs) has been made in this study. Combination of three essential growth factors: epidermal growth factor (EGF), keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) could demonstrate successfully induction of hSKPs to differentiation into corneal cells.The induced cells expressed the appearance of markers of corneal epithelial cells as shown by the presence of keratin 3 (K3) by antibody label and Western blot assay. The K3 gene expression of induced hSKPs cells as shown by reverse transcription-polymerase chain reaction (RT-PCR) technology was also demonstrated. The presence of these markers at both gene and protein levels could lead to our conclusion that the directional transdifferentiation of hSKPs cells into corneal epithelial cells was successfully done under this cell induction protocol. The finding shows a newly available stem cell source can be obtained from easily available skin. Cells from autologous human skin might be used for corneal disorder treatment in future clinical application.

关键词： corneal epithelial-like cell human skin-derived precursor cell transdifferentiation

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Comparative study of carbonic anhydrase activity in waters among different geological eco-environments of Yangtze River basin and its ecological significance

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Journal of Environmental Sciences 2018年第4期30卷 173-181页

作者： sila Onesmus Nzung'a Weizhi Pan Taiming Shen Wei Li Xiaoqun Qin Chenwei Wang Liankai Zhang Longjiang Yu Institute of Resource Biology and Biotechnology Department of Biotechnology College of Life Science & Technology Huazhong Universityof Science & Technology Wuhan 430074 China Institute of Karst Geology Chinese Academy of Geological Sciences Guilin 541004 China Key Laboratory of Karst Dynamics Ministry of Land and Resources/Guangxi Zhuang Autonomous Region Guilin 541004 China Teachers Searvice Commission-Kenya P.O. Box Private Bag 00100 Nairobi Kenya

This study provides the presence of carbonic anhydrase（CA） activity in waters of the Yangtze River basin, China, as well as the correlation of CA activity with HCO-3 concentration and CO2 sink flux. Different degrees of CA activity could be detected in almost all of the water samples from different geological eco-environments in all four seasons. The CA activity of water samples from karst areas was significantly higher than from non-karst areas（PP3-concentration（r = 0.672, P2 sink flux（r = 0.602, P = 0.076） in karst areas. This suggests that CA in waters might have a promoting effect on carbon sinks for atmospheric CO2 in karst river basins. In conditions of similar geological type, higher CA activity was generally detected in water samples taken from areas that exhibited better eco-environments, implying that the CA activity index of waters could be used as an indicator for monitoring ecological environments and protection of river basins. These findings suggest that the role of CA in waters in the karst carbon sink potential of river basins is worthy of further in-depth studies.

关键词： Yangtze River basin Carbon sink Eco-environments Water body Carbonic anhydrase(CA)

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Development and validation of RP-HPLC method for determination of acetazolamide, furosemide and phenytoin extemporaneous suspensions

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 138-139页

作者： Warisada sila-on Salinee Na-Ranong Sirilak Rakrod Sarote Ornlaor Zongporn Joungmunkong Ubon Ratchathani University Ubon Ratchathani 34190Thailand Division of Pharmacy Sappasit Prasong HospitalUbon RatchathaniThailand

The development of the extemporaneous preparations allows physicians to adjust the dose for pediatric patients and provides for a more convenient dosage vehicle for those patients with difficulty swallowing tablets[1].As such,the production unit of pharmacy division,Sappasit Prasong Hospital,Ubon Ratchathani province,prepared the extemporaneous formulations such as Acetazolamide(AM),Furosemide(FM)and Phenytoin(PT)powder for *** extemporaneous suspensions of 10 mg/mL AM,2 mg/mL FM and 10 mg/mL PT were prepared from 250 mg Diamox?,40 mg Lasix?and 50 mg Dilantin?tablets,respectively and diluted with syrup vehicle.

关键词： Development and validation of RPHPLC method Extemporaneous suspensions

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Sonographic Estimation of Visceral Adipose Tissue in an African Population

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Health 2015年第5期7卷 653-658页

作者： Thomas Adejoh Idigo Felicitas Ugochinyere Sobechukwu Warric Iwene Onwuzu Nkubli Flavious Bobouin Alhamdu sila Moi Radiology Department Nnamdi Azikiwe University Teaching Hospital Nnewi Nigeria Department of Medical Radiography and Radiological Sciences University of Nigeria Enugu Campus Enugu Nigeria Medical Imaging Unit Department of Medical Center University of Nigeria Nsukka Nigeria Department of Medical Radiography College of Medical Sciences University of Maiduguri Maiduguri Nigeria

Purpose: To establish normal values of sonographically-quantified visceral adipose tissue (VAT) in an African population and to correlate results with biomarkers. Patients and Methods: 100 male and 150 female volunteers were scanned for intra-abdominal adipose tissue thickness. Other parameters obtained include BMI, WC, and blood pressure (BP). Pearson correlation coefficient (r) was used to estimate the degree of relationship in VAT, BMI, WC, and blood pressure. Results: The mean VAT thickness was 25.36 ± 16.42 mm. VAT correlated positively and linearly with age (r = 0.651;p < 0.05), BMI (males: r = 0.745, p < 0.05, and females: r = 0.736;p < 0.05), WC (males: r = 0.797, p < 0.05;females: r = 0.779, p < 0.05), and systolic and diastolic blood pressure. (Systolic: r = 0.524, p < 0.05, and diastolic: r = 0.535, p <0.05). Conclusion: Ultrasound is a useful tool in assessing VAT during routine scans to identify patients at risk of increased blood pressure and cardiac diseases associated with obesity.

关键词： Adipose Tissue Blood Pressure Viscera Ultrasound

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长江流域不同地质生态环境土壤碳酸酐酶活性、有机碳含量及其相关性

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中国岩溶 2016年第2期35卷 173-178页

作者：潘伟志 sila Onesmus Nzung’a 申泰铭李为王晨玮余龙江华中科技大学生命科学与技术学院生物技术系/资源生物学与生物技术研究所湖北武汉430074

为了探讨流域岩溶生态系统土壤碳酸酐酶（CA）活性、有机碳含量及CA固碳之间的潜在关系,选取长江流域干流及支流沿岸不同地质生态环境下的10个样地,比较长江流域不同地质生态环境下表层土壤（0--20cm）中的碳酸酐酶（CA）活性与土壤有... 详细信息

为了探讨流域岩溶生态系统土壤碳酸酐酶（CA）活性、有机碳含量及CA固碳之间的潜在关系,选取长江流域干流及支流沿岸不同地质生态环境下的10个样地,比较长江流域不同地质生态环境下表层土壤（0--20cm）中的碳酸酐酶（CA）活性与土壤有机碳（SOC）含量,并分析二者之间的关系。结果表明：（1）长江流域岩溶区的表层土壤CA活性要高于非岩溶区（P〈0.01）,土壤CA活性的差异与样地的地质类型及植被类型不同有关;（2）位于岩溶区高场（GC）样地的年平均SOC含量最高（1.09%）,而位于非岩溶区外洲（WZ）样地的年平均SOC含量最低（0.29%）,而且总体比较而言,长江流域冬季表层土壤的平均SOC含量显著高于夏季（P〈0.01）;（3）相关性分析结果显示,不同季节长江流域岩溶区表层土壤CA活性与SOC含量呈一定的正相关。研究结果为进一步研究流域岩溶生态系统土壤CA与土壤固碳能力之间的关系奠定了基础。

关键词：长江流域不同地质生态环境碳酸酐酶(CA) 土壤有机碳(SOC) 岩溶区

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The Ecological Importance and Influence of Land Use on Carbonic Anhydrase Activity of Waters in the Upstream Section of the Wujiang River Basin

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Journal of Resources and Ecology 2015年第4期6卷 230-236页

作者：潘伟志 sila Onesmus Nzung'a 李为黄奇波申泰铭王晨玮覃小群余龙江华中科技大学生命科学与技术学院生物技术系资源生物学与生物技术研究所武汉430074 Teachers Service Commission-Kenya 中国地质科学院岩溶地质研究所桂林541004 国土资源部/广西壮族自治区岩溶动力学重点实验室桂林541004

Thirty-four sampling sites for surface water and twenty-seven sampling sites for ground water in areas of different land use were selected in the Wujiang River Basin, upstream section in Guizhou Province, China. Carbonic anhydrase （CA） activity in surface and ground water was analyzed and compared. The results show that CA activity was detected in all surface and ground water samples. In general, average CA activity in surface and ground water was highest from samples in arbor lands, followed by shrub lands, shrub and grass lands, agricultural lands, and residential areas. The average CA activity in ground water from arbor lands, shrub lands, and shrub and grass lands was higher than that in surface water. Water CA activity was positively correlated with HCO3- concentration in surface water and ground water. These results imply that the contribution of CA in water to the karst carbon sink potential of river basins warrants further extensive and in-depth research.

关键词： Wujiang River basin land use type carbon sink carbonic anhydrase (CA) ground water surface water

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校园文化建设对国防生的教育培养探究

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内蒙古师范大学学报（教育科学版） 2010年第7期23卷 43-45页

作者：陈斯拉华南师范大学文学院广东广州510006

国防生作为校园中新生的群体,"军味"校园文化活动对国防生的成才有着重要的作用。通过开展有针对性的校园文化活动,可以促进国防生的全面成才,不断坚定他们的思想信念和成才意志,在校园文化活动的实践中增强对国防生的教育引导作用,切... 详细信息

国防生作为校园中新生的群体,"军味"校园文化活动对国防生的成才有着重要的作用。通过开展有针对性的校园文化活动,可以促进国防生的全面成才,不断坚定他们的思想信念和成才意志,在校园文化活动的实践中增强对国防生的教育引导作用,切实提高思想政治教育工作的效能。

关键词：校园文化建设国防生军味教育培养

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